Métodos de conservación de un preparado antianémico a base sangre

Se estudian lso metodos empleados para la conservacion de un prteparado antianemico a base de sangre. Se analizaron las caracteristicas fisicoquimicas, microbiologicas y sensoriales del producto. Debido a los obstaculos usados para su conservacion, el producto posee una alta calidad higienica, pero esta puede ser mejorada mediante el uso de la energia ionizante. Se aislo e identifico la microflora presente. Se realizo un estudio radiobiologico de las cepas aisladas y se determino la dosis de reduccion decimal del microorganismo mas resistente. Se aplicaron dosis de 1 kGy para eliminar 10 3 cel/g de la cepa. No se produjeron cambios en las caracteristicas fisicoquimicas de las muestras despues de su irradiacion. Se detectaron cambios en las propiedades sensoriales del producto irradiado, pero este no es rechazable. Las radiaciones gamma mejoran la calidad higienica del producto (AU)

Identification of bacteria in water for pharmaceutical use.

Different systems for the obtention of water used in Biopharmaceutical Industry were characterized from the bacteriological point of view. Determination of aerobic mesophilic microorganisms was performed; as well as the isolation of contaminant microorganisms for what the techniques of membrane filtration was used. For the identification of the more representative species there were made conventional biochemical tests and quick systems: API. The results show that water serving as tap water for purification systems fulfill with the microbiological requirements to this kind of water. All the isolated microorganisms were gram-negative bacteria characteristics of this environment: Pseudomonas putida, Xanthomonas maltophilia, Aeromonas salmonicida ssp. salmonicida, Flavimonas coryzihabitans and Acinetobacter iwoffi. The ultrafiltration and distillation tested systems fulfill with the established microbiological limits, except for deionization and distilled water storing systems. The isolation showed that approximately the 76.9% were of Gram-negative bacteria, the 14.6% of Gram-positive cocci and the 8.5% Gram-positive sporulated bacilli. The most representative genus of purified water were: Pseudomonas, with the higher percent of incidence, Staphylococcus, Bacillus, Flavobacterium, Sphingomonas, Aeromonas and Agrobacterium.