RESUMO
1. The aim of the present study was to evaluate the antimicrobial resistance of Campylobacter strains (C. jejuni, C. coli and C. lari) isolated from broiler carcasses processed in the State of Paraná, Brazil. 2. Rates of microbial resistance and susceptibility were assessed by both Disk Diffusion (DD) and Etest (Minimum Inhibitory Concentration) techniques. Antibiotics were tested using DD (12 antibiotics) and/or MIC (7 antibiotics) methods. 3. A total of 95.8% of the strains were resistant to at least two agents. In terms of multidrug resistance, 75% of strains were resistant to three or more groups of antibiotics. The highest rates of resistance were detected for cefalotin, ciprofloxacin, tetracycline and nalidixic acid. A high rate of susceptibility of the strains to erythromycin (95.8%) was found confirming that this is considered the agent of choice for treating campylobacteriosis. Comparison of the microbial resistance and susceptibility, as determined simultaneously by the two methods, found the techniques to be statistically equivalent for 5 out of the 6 antibiotics tested. 4. The results of this study suggest the need for adopting measures to control the use of antibiotics in broiler production to prevent multidrug resistance of Campylobacter strains and reduce the risk of serious human diseases caused by the consumption of contaminated chicken meat.
Assuntos
Antibacterianos/farmacologia , Infecções por Campylobacter/veterinária , Campylobacter/efeitos dos fármacos , Galinhas , Farmacorresistência Bacteriana Múltipla , Doenças das Aves Domésticas/microbiologia , Animais , Infecções por Campylobacter/epidemiologia , Infecções por Campylobacter/microbiologia , Campylobacter coli/efeitos dos fármacos , Campylobacter jejuni/efeitos dos fármacos , Campylobacter lari/efeitos dos fármacos , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão , Testes de Sensibilidade Microbiana , Doenças das Aves Domésticas/epidemiologiaRESUMO
The aim of this study was the detection of Campylobacter sp. in raw chicken sausages using the methods ISO 10272-1 and ISO 10272-2. The overall prevalence of Campylobacter sp. in the samples tested was 16.67%, representing a serious risk to the health of consumers, particularly if measures guaranteeing proper cooking of foods and prevention of cross-contamination are not adopted. Furthermore, the majority of campylobacteriosis cases in humans are caused by consumption or improper handling of contaminated raw or undercooked poultry meat, which constitute the main vehicle of this infection.
Assuntos
Animais , Humanos , Campylobacter/isolamento & purificação , Produtos da Carne/microbiologia , Brasil/epidemiologia , Galinhas , Doenças Transmitidas por Alimentos/epidemiologia , Técnicas Microbiológicas/métodos , Prevalência , Medição de RiscoRESUMO
The aim of this study was the detection of Campylobacter sp. in raw chicken sausages using the methods ISO 10272-1 and ISO 10272-2. The overall prevalence of Campylobacter sp. in the samples tested was 16.67%, representing a serious risk to the health of consumers, particularly if measures guaranteeing proper cooking of foods and prevention of cross-contamination are not adopted. Furthermore, the majority of campylobacteriosis cases in humans are caused by consumption or improper handling of contaminated raw or undercooked poultry meat, which constitute the main vehicle of this infection.
Assuntos
Campylobacter/isolamento & purificação , Produtos da Carne/microbiologia , Animais , Brasil/epidemiologia , Galinhas , Doenças Transmitidas por Alimentos/epidemiologia , Humanos , Técnicas Microbiológicas/métodos , Prevalência , Medição de RiscoRESUMO
This study was carried out comparing the conventional methods (ISO 11290-1 and BAM method, 2008) and system mini-Vidas® (Biomerieux), for detection of Listeria sp. and Salmonella sp. in cooled sausage. The immunoenzymatic method has shown to be effective for the detection of target pathogens, it has presented itself as an excellent screening method.
Assuntos
Microbiologia de Alimentos/métodos , Listeria/isolamento & purificação , Salmonella/isolamento & purificação , Ensaio de Imunoadsorção Enzimática/métodos , Programas de Rastreamento/métodos , Sensibilidade e EspecificidadeRESUMO
This study was carried out comparing the conventional methods (ISO 11290-1 and BAM method, 2008) and system mini-Vidas® (Biomerieux), for detection of Listeria sp. and Salmonella sp. in cooled sausage. The immunoenzymatic method has shown to be effective for the detection of target pathogens, it has presented itself as an excellent screening method.
