RESUMO
To determine the importance of Norwalk-like viruses (NLVs) as pediatric pathogens in a developing country, the seroprevalence and seroincidence of this group of viruses in a cohort of children less than 4 years of age in an urban shantytown in northeastern Brazil was examined. Serum samples were collected approximately every 6 months from 135 children who were surveyed three times each week for diarrhea and vomiting. NLV IgG was measured by an enzyme immunosorbent assay (EIA) with recombinant Norwalk virus capsid protein. Overall NLV seroprevalence was 71%, and the overall NLV seroconversion rate was 0.7 seroconversions per child-year. The highest age-specific NLV seroconversion rate (0.8 seroconversions per child-year) was observed in the 13-24-month age group. For all study children, the incidence of diarrhea and vomiting was significantly greater (P < 0.01) during time periods spanned by serum pairs that indicated NLV seroconversion compared with time periods without NLV seroconversion. However, NLV seroconversion was not associated with gastrointestinal symptoms during the first year of life.
Assuntos
Infecções por Caliciviridae/epidemiologia , Proteínas do Capsídeo , Capsídeo/imunologia , Brasil/epidemiologia , Infecções por Caliciviridae/imunologia , Infecções por Caliciviridae/patologia , Pré-Escolar , Diarreia/epidemiologia , Diarreia/virologia , Feminino , Gastroenterite/epidemiologia , Gastroenterite/virologia , Humanos , Técnicas Imunoenzimáticas , Incidência , Lactente , Masculino , Estudos Prospectivos , Estudos Soroepidemiológicos , Testes Sorológicos , Vômito/epidemiologia , Vômito/virologiaRESUMO
Rotavirus nonstructural protein NSP4 has recently been suggested to function as a viral enterotoxin and play a role in the pathophysiological mechanism whereby rotaviruses induce diarrhea. The ability of rotavirus NSP4 to stimulate a humoral immune response was examined in naturally infected children and adults, rotavirus vaccinated children, as well as a cellular immune response in adults. In this study, 10 of 10 naturally infected children and 9 of 10 rotavirus-vaccinated children showed a weak humoral IgG immune response to recombinant NSP4 (rNSP4) and/or a synthetic peptide corresponding to residues 114-134 of NSP4. Modest serum IgG antibody responses were detected in 20 of 20 adults. A cellular immune response to rNSP4 and/or NSP4(114-134) were detected in 8 of 10 adults measured either as a T-cell proliferative response (7 of 10), an increased production of IL-2 (6 of 10), or increased production of interferon-gamma (8 of 10). These results indicate that NSP4 induces a humoral immune response in humans and show for the first time that NSP4 stimulates a cellular immune response, possibly including cytolytic T-cells.
Assuntos
RNA Polimerases Dirigidas por DNA , Infecções por Rotavirus/imunologia , Rotavirus/química , Proteínas não Estruturais Virais/imunologia , Adulto , Anticorpos Antivirais/sangue , Finlândia , Humanos , Imunidade Celular/imunologia , Imunoglobulina A/sangue , Imunoglobulina G/sangue , Lactente , Nicarágua , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/imunologia , Rotavirus/imunologia , Infecções por Rotavirus/virologia , Suécia , Linfócitos T/metabolismo , Proteínas não Estruturais Virais/biossínteseRESUMO
A cDNA obtained from Grimsby virus (GRV), a Norwalk-like virus, purified from a stool sample of a symptomatic adult associated with a gastroenteritis outbreak in the United Kingdom, was used to obtain the complete nucleotide sequence of the second open reading frame (ORF2). The ORF2 sequence of GRV predicts a capsid of 539 amino acids (aa) which exhibits aa identities of 96% to Lordsdale virus, 67% to Mexico virus (MXV), and 43% to Norwalk virus (NV). The GRV capsid protein was expressed in insects cells by using a recombinant baculovirus, and the resulting virus-like particles (VLPs) possessed a protein with an apparent molecular weight of 58,000. Hyperimmune antisera raised against purified GRV, MXV, and NV VLPs were tested in an indirect enzyme-linked immunosorbent assay (ELISA) against GRV, NV, and MXV VLPs, revealing that GRV is antigenically distinct from both NV and MXV. The antigenic specificity of the GRV-hyperimmune antiserum was confirmed in an antigen capture ELISA using GRV-, NV-, or MXV-containing fecal specimens. The expression of the GRV capsid protein has, for the first time, allowed the antigenic comparison of three distinct recombinant Norwalk-like viruses.
Assuntos
Antígenos Virais/genética , Caliciviridae/genética , Caliciviridae/imunologia , Vírus Norwalk/genética , Vírus Norwalk/imunologia , Adulto , Sequência de Aminoácidos , Anticorpos Antivirais , Antígenos Virais/química , Caliciviridae/crescimento & desenvolvimento , Infecções por Caliciviridae/virologia , Capsídeo/química , Capsídeo/genética , Capsídeo/imunologia , DNA Complementar/genética , DNA Complementar/isolamento & purificação , DNA Viral/genética , DNA Viral/isolamento & purificação , Gastroenterite/virologia , Expressão Gênica , Genes Virais , Humanos , Microscopia Eletrônica , Dados de Sequência Molecular , Peso Molecular , Vírus Norwalk/crescimento & desenvolvimento , Fases de Leitura Aberta , Homologia de Sequência de Aminoácidos , Especificidade da EspécieRESUMO
Norwalk virus (NV) and Mexico (MX) virus represent distinct genetic clusters within the same genus of human caliciviruses (CVs), a major cause of diarrhea in adults. The magnitude and potential risk factors of human CV infection in populations from Santiago and Punta Arenas, Chile, were assessed. Individuals (n = 1,864) gave a blood sample and answered a questionnaire during a household survey. Sera were tested for antibody to NV and MX virus with use of recombinant capsid antigens. Overall, NV and MX virus seroprevalence rates were 83% and 91% in Santiago vs. 67% and 90% in Punta Arenas, respectively (P < .001 for NV virus). Lower socioeconomic status and increasing age were risk factors for infection with both viruses (P < .001). Consumption of seafood, consumption of vegetables, and child care center attendance were population risk factors for infection, but the association of a factor with a virus depended on the city. Prevention of human CV infections will require individual assessment in different communities.
Assuntos
Infecções por Caliciviridae/epidemiologia , Vírus Norwalk , Adolescente , Adulto , Fatores Etários , Anticorpos Antivirais/sangue , Caliciviridae , Infecções por Caliciviridae/sangue , Infecções por Caliciviridae/imunologia , Criança , Pré-Escolar , Chile/epidemiologia , Cidades/epidemiologia , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Vírus Norwalk/imunologia , Fatores de Risco , Estudos Soroepidemiológicos , Classe SocialRESUMO
The presence of antibodies against Norwalk virus (NV) was studied in sera from different Venezuelan populations, using an enzyme immuno-assay (EIA) based on recombinant NV protein. Antibodies to NV were found in 47%-53% of urban subjects from Caracas, 83% of rural subjects from the west of the country, and 73%-93% of Amerindian subjects. The prevalences found in the rural and Amerindian groups were significantly higher than that in the urban group. Although about 50% of the children studied were seropositive for NV by the age of 5 years, only four (0.4%) of 1120 faecal samples from children with diarrhoea which were tested for the presence of NV antigen by sandwich EIA were found positive. An increase of at least 4-fold in the titre of anti-NV IgA was found in three (5%) of 61 pairs of sera taken during and 1 month after an acute episode of diarrhoea not due to rotavirus. NV was therefore not a predominant aetiological cause of gastro-enteritis in young children in Venezuela between 1993 and 1995, although it can be the cause of diarrhoea in infants.
Assuntos
Infecções por Caliciviridae/epidemiologia , Gastroenterite/virologia , Vírus Norwalk/imunologia , Adolescente , Adulto , Anticorpos Antivirais/análise , Criança , Pré-Escolar , Fezes/virologia , Feminino , Humanos , Técnicas Imunoenzimáticas , Indígenas Sul-Americanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Vírus Norwalk/isolamento & purificação , Gravidez , Prevalência , Venezuela/epidemiologiaRESUMO
Small round-structured viruses (SRSVs) or Norwalk-like viruses have been implicated as an important causative agent of gastroenteritis outbreaks. We used the relatively newly developed recombinant enzyme immunoassays (EIAs) to determine the seroprevalence of Norwalk virus (NV) and Mexico virus (MxV) in a family-based cohort and an antenatal clinic cohort at Ga-Rankuwa Hospital, Ga-Rankuwa, South Africa. High prevalences (96 to 99%) of anti-NV and anti-MxV antibodies were detected in both cohorts. We also investigated the pattern of antibody acquisition in a cohort of infants and young children without gastroenteritis and found that by 48 months of age all children had acquired adult antibody levels to both these viruses. Lastly, we tested 276 stool specimens collected from infants and young children with gastroenteritis for the presence of NV or MxV antigen by recombinant EIAs to each virus, by electron microscopy (EM), and by reverse transcription (RT)-PCR. NV and MxV antigens were present in 1.8 and 4.3% of the stool specimens, respectively, by the recombinant EIAs; 9.2% were positive for SRSVs by EM, and 25% of these SRSVs gave a positive result by RT-PCR for primer pair 35-36 directed to a region of the RNA-dependent RNA polymerase gene. The seroprevalence studies indicate a high level of exposure to these viruses in both children and adults. Although the viral antigens are not highly prevalent in diarrheal stools, it was determined by the two assays for NV and MxV that children are, nevertheless, infected early in life.
Assuntos
Anticorpos Antivirais/análise , Infecções por Caliciviridae/epidemiologia , Caliciviridae/imunologia , Gastroenterite/virologia , Vírus Norwalk/imunologia , Adolescente , Adulto , Idoso , Sequência de Aminoácidos , Antígenos Virais/isolamento & purificação , Caliciviridae/genética , Caliciviridae/isolamento & purificação , Infecções por Caliciviridae/imunologia , Capsídeo/genética , Capsídeo/imunologia , Criança , Pré-Escolar , Surtos de Doenças , Fezes/virologia , Feminino , Gastroenterite/epidemiologia , Humanos , Técnicas Imunoenzimáticas , Masculino , Microscopia Eletrônica , Pessoa de Meia-Idade , Dados de Sequência Molecular , Vírus Norwalk/genética , Vírus Norwalk/isolamento & purificação , Filogenia , Reação em Cadeia da Polimerase , Prevalência , RNA Polimerase Dependente de RNA/genética , Proteínas Recombinantes/imunologia , Sensibilidade e Especificidade , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Estudos Soroepidemiológicos , África do Sul/epidemiologiaRESUMO
BACKGROUND: Rotavirus is the leading cause of severe diarrhea in infants. To provide a base line for assessing the efficacy of rotavirus vaccines, we evaluated the protection that is conferred by natural rotavirus infection. METHODS: We monitored 200 Mexican infants from birth to two years of age by weekly home visits and stool collections. A physician assessed the severity of any episodes of diarrhea and collected additional stool specimens for testing by enzyme immunoassay and typing of strains. Serum collected during the first week of life and every four months thereafter was tested for antirotavirus IgA and IgG. RESULTS: A total of 316 rotavirus infections were detected on the basis of the fecal excretion of virus (56 percent) or a serologic response (77 percent), of which 52 percent were first and 48 percent repeated infections. Children with one, two, or three previous infections had progressively lower risks of both subsequent rotavirus infection (adjusted relative risk, 0.62, 0.40, and 0.34, respectively) and diarrhea (adjusted relative risk, 0.23, 0.17, and 0.08) than children who had no previous infections. No child had moderate-to-severe diarrhea after two infections, whether symptomatic or asymptomatic. Subsequent infections were significantly less severe than first infections (P=0.024), and second infections were more likely to be caused by another G type (P=0.054). CONCLUSION: In infants, natural rotavirus infection confers protection against subsequent infection. This protection increases with each new infection and reduces the severity of the diarrhea.
Assuntos
Diarreia Infantil/microbiologia , Infecções por Rotavirus/imunologia , Anticorpos Antibacterianos/sangue , Fatores de Confusão Epidemiológicos , Diarreia Infantil/classificação , Diarreia Infantil/imunologia , Fezes/virologia , Humanos , Imunidade Inata , Lactente , Recém-Nascido , Estudos Longitudinais , Recidiva , Risco , Rotavirus/classificação , Rotavirus/imunologia , Rotavirus/isolamento & purificação , Infecções por Rotavirus/microbiologiaRESUMO
The seroepidemiology of Norwalk virus infections was examined among Amerindians belonging to eight relatively isolated communities in the Amazon region by means of a new enzyme immunoassay using recombinant Norwalk virus antigen. The seroprevalence of antibodies to Norwalk virus ranged from 39% in the Maiogong to 100% in the Kubenkrankrein. The distribution of antibody levels varied greatly among groups; five of the eight communities had an antibody prevalence greater than 90% with many high values (> 100 units), while three had both a low seroprevalence and a preponderance of low values (< 100 units). While few children less than 5 years of age were sampled, no significant differences in antibody prevalence were noted among age groups, and the prevalence of antibody among children 5-10 years of age approached that of the older age groups. The low prevalence of titers of antibodies to Norwalk virus in several tribes living in these isolated Indian communities suggests that Norwalk virus may have been only recently introduced.
Assuntos
Anticorpos Antivirais/sangue , Infecções por Caliciviridae/epidemiologia , Gastroenterite/epidemiologia , Indígenas Sul-Americanos , Vírus Norwalk/imunologia , Adolescente , Adulto , Brasil/epidemiologia , Criança , Pré-Escolar , Humanos , Técnicas Imunoenzimáticas , Prevalência , Estudos Soroepidemiológicos , Venezuela/epidemiologiaRESUMO
Bovine rotavirus T449 was isolated from feces of a calf with diarrhea. Serological characterization by serotype-specific monoclonal antibodies showed that the T449 virus belonged to serotype 1. This is the first report of a bovine rotavirus that does not belong to serotype 6, 8, or 10. The serotype 1 designation was confirmed by using an immunoperoxidase focus neutralization assay. The gene encoding the major neutralization antigen (VP7) was cloned and its nucleotide sequence was determined. The sequence obtained was 1062 bp in length and contained an open reading frame corresponding to 326 amino acid residues. Comparative analysis of the deduced amino acid sequence with the corresponding sequence of the human serotype 1 rotavirus strain, Wa, revealed a 90% identity. When compared to the predicted amino acid sequence of VP7 protein of the other serotypes an overall divergence of 20 to 25% was detected. These data show that the serological typing agrees with the result of the genetic analysis.
Assuntos
Antígenos Virais , Proteínas do Capsídeo , Capsídeo/genética , Capsídeo/imunologia , Genes Virais , Rotavirus/imunologia , Proteínas Estruturais Virais/genética , Sequência de Aminoácidos , Animais , Anticorpos Monoclonais/imunologia , Bovinos , Doenças dos Bovinos/microbiologia , Diarreia/veterinária , Dados de Sequência Molecular , Rotavirus/genética , Alinhamento de Sequência , SorotipagemRESUMO
Some rotavirus strains, including vaccine candidates, have been demonstrated to cause hepatitis in immunodeficient and malnourished mice and to grow in human liver cells. To determine whether rotavirus spreads outside the intestine in naturally infected children, we examined tissues from four immunodeficient children affected with severe combined immunodeficiency disease, acquired immunodeficiency disease syndrome, or DiGeorge syndrome. Chronic rotavirus-related diarrhea, which persisted until death, had also developed in each child. Using indirect immunoperoxidase techniques, we identified rotavirus antigen in the liver and kidney with a hyperimmune guinea pig antiserum prepared to double-shelled rotavirus particles. Similar immunostaining with an antiserum to a rotavirus nonstructural protein (NS26) provided evidence of active virus replication. The observed reactivity was eliminated specifically when serial sections were immunostained with the same antiserum that had been absorbed with either double-shelled rotavirus particles or NS26. Immunostaining was not observed in the liver of children with other diseases (alpha 1-antitrypsin deficiency, inspissated bile syndrome, and acute rejection of a transplanted liver). These findings demonstrate that rotavirus infections in children can extend beyond the intestinal tract. Further studies are warranted to determine whether extraintestinal rotavirus replication occurs in children without severe immunodeficiency, such as malnourished children.
Assuntos
Síndrome da Imunodeficiência Adquirida/complicações , Síndrome de DiGeorge/complicações , Hepatite Viral Humana/microbiologia , Nefropatias/microbiologia , Infecções por Rotavirus/complicações , Imunodeficiência Combinada Severa/complicações , Diarreia Infantil/microbiologia , Feminino , Humanos , Técnicas Imunoenzimáticas , Lactente , Masculino , Rotavirus/isolamento & purificação , Rotavirus/fisiologia , Replicação ViralRESUMO
Two porcine rotavirus strains (CN86 and CC86) isolated during an epidemiological survey of diarrhoea in swine in Argentina were studied because of several unique characteristics. Both these strains were isolated and cloned from the same faecal sample and the electrophoretic migration of 10 of their 11 genomic dsRNA genomic segments in polyacrylamide gels was identical, but strain CC86 had a supershort electropherotype. We analysed biochemical, serological and biological properties of both viruses. In vitro translation of genome segment 11 RNAs showed that both viruses produced a polypeptide with an apparent Mr of 26K. No differences in any of the other virus-induced proteins made in infected MA104 cells were found on one- and two-dimensional gels for either strain. In addition, the serotype and the subgroup specificities of both viruses were identical (group A, subgroup I, serotype 5). These results suggest that the rearranged strain was probably generated from the standard one and that the coding capacity of the rearranged segment was conserved. Consistent with this hypothesis, primer extension analysis revealed that the supershort strain had a rearrangement involving partial duplication of genomic segment 11. Biological studies showed differences between these viruses. The rearranged strain (CC86) produced larger plaques in monolayers of MA104 cells and outgrew the standard strain (CN86) when cells were coinfected with both viruses at different relative concentrations and different m.o.i. The possibility that large plaque formation and efficient virus replication can be influenced by the products of genomic segment 11, in addition to segment 4, is discussed.
Assuntos
Rearranjo Gênico , RNA de Cadeia Dupla/análise , RNA Viral/análise , Rotavirus/genética , Animais , Eletroforese em Gel de Poliacrilamida , Fezes/microbiologia , Genes Virais , Fenótipo , Biossíntese de Proteínas , Rotavirus/crescimento & desenvolvimento , Suínos , Ensaio de Placa ViralRESUMO
Human rotaviruses are the major, recognized cause of infantile diarrhea worldwide. Characterization of naturally occurring human isolates indicates that there are six human rotavirus serotypes, four of which (serotype 1 to 4) are widespread. We utilized monoclonal antibodies specific for the VP of serotypes 1, 2, 3, and 4 as capture antibody in a sandwich enzyme-linked immunosorbent to serotype rotaviruses directly in stool samples. The stool samples were collected from 1983 through 1986, from two epidemiologic studies in the area of Buenos Aires, Argentina. All four serotypes assayed were found Serotype 2 and 3 viruses, which were detected most frequently in 1983 and 1984, were virtually undetected in 1985 and 1986 (chi 2 = 23, P = less than 0.001 for this difference). No significant difference was noted among the three collection sites for serotype prevalence. These results indicate that the changing predominance of rotavirus serotype in a given region can involve multiple serotypes at the same time. Analysis of an outbreak of diarrhea in two neighboring families which occurred during a prospective study of community diarrhea documented inter- and intra-family spread of one serotype of virus.
Assuntos
Anticorpos Monoclonais/imunologia , Anticorpos Antivirais/imunologia , Rotavirus/classificação , Argentina/epidemiologia , Diarreia/epidemiologia , Diarreia/microbiologia , Surtos de Doenças , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Fezes/microbiologia , Humanos , RNA de Cadeia Dupla/isolamento & purificação , RNA Viral/isolamento & purificação , SorotipagemRESUMO
Bovine rotaviruses isolated from beef and dairy herds in Argentina were serotyped by the immunoperoxidase focus reduction assay as previously described (G. Gerna, M. Battaglia, G. Milenesi, N. Passarani, E. Percivalle, and E. Cattaneo, Infect. Immun. 43:722-729, 1984). Three strains from beef herds were related to the UK and NCDV bovine rotavirus strains defined as serotype 6 (Y. Hoshino, R. G. Wyatt, H. B. Greenberg, J. Flores, and A. Z. Kapikian, J. Infect. Dis. 149:694-702, 1984). Two other strains from dairy herds were classified as bovine viruses related to the bovine B223 strain reported by Woode and co-workers (G. N. Woode, N. E. Kelso, T. F. Simpson, S. K. Gaul, L. E. Evans, and L. Babiuk, J. Clin. Microbiol. 18:358-364, 1983) in the United States. A serotyping antibody-capture enzyme-linked immunoassay to detect serotype 6 rotavirus using a serotype 6-specific monoclonal antibody was developed and evaluated for strain characterization. Characterization of 72 group A rotavirus-positive fecal samples from beef herds and 43 fecal samples from dairy herds showed a predominance of serotype 6 rotavirus in beef herds but both serotype 6 and non-serotype 6 rotaviruses in dairy herds. Analysis of genomic double-stranded RNA by polyacrylamide gel electrophoresis showed that when outbreaks were caused by one serotype only a single electropherotype was present in all samples.
Assuntos
Doenças dos Bovinos/microbiologia , Infecções por Rotavirus/veterinária , Rotavirus/classificação , Animais , Variação Antigênica , Argentina , Bovinos , Reações Cruzadas , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Técnicas Imunoenzimáticas , Testes de Neutralização , RNA Viral/análise , Rotavirus/genética , Rotavirus/imunologia , Infecções por Rotavirus/microbiologia , SorotipagemRESUMO
Between April 1979 and December 1982, viral agents were found in 231 of 695 children admitted to the Texas Children's Hospital with gastroenteritis. Electron microscopic analysis showed that rotaviruses were the most common viral agents, and a seasonal pattern of rotavirus disease was observed. The migration patterns of the RNA segments of these rotaviruses on electrophoresis in polyacrylamide gels were compared with those of rotaviruses collected from other areas of the United States of America and from Argentina, Colombia and Mexico. A number of different RNA electropherotypes were found, including some patterns not previously reported.