Egg white protein hydrolysate ameliorated sepsis-induced inflammatory injuries in kidney and liver based on metabolomics analysis.

Sepsis caused by infection could induce systemic inflammation and various organ damages. Bioactivities of peptides derived from eggs were proved by lots of researchers; hence they might be further developed for therapeutic use. In this study, the hydrolysate named "PEP" was obtained by enzymatic hydrolysis and purification. Rats were divided into control, LPS, PEP1 and PEP2 groups. The rat model was established using lipopolysaccharide (LPS) at 10 mg/kg by intraperitoneal injection. The results of blood counts, pro-inflammatory cytokines (TNF-α and IL-6), BUN and Cr levels and pathological damage in tissue or serum suggested that PEP alleviated LPS-induced injuries. Plasma metabolites of PEP were analyzed and identified using UPLC-MS/MS, which revealed that seven pathways might play critical roles in the protection process, involved in oxidative stress, energy and amino acid metabolism. Our data suggest that PEP ameliorated LPS-induced acute liver and kidney injuries, which is helpful for further development and research.

Effect of Marine Capsules on BALB/c Human Hepatocellular Carcinoma Cell Line SMMC-7721 in vitro / 中国药师

Objective:To observe the effect of marine capsules on BALB/c human hepatocellular carcinoma cell line SMMC-7721 in vitro. Methods:Human hepatoma SMMC-7721 cell line was cultured in vitro to establish a hepatoma tumor model, and was random-ly divided into the model control group, 5-Fu group (25 mg·kg-1), marine capsules high dose group (90 mg·kg-1), marine cap-sules low dose group (45 mg·kg-1 ) and the blank control group with 10 mice in each and 14-day continuous administration. Blood was taken from the eyeball and serum was isolated. The tumor strains were picked, the wet weight was obtained, the inhibition rate was calculated, and the pathological changes of tumor tissues were observed after HE staining. Results: Compared with that in the model control group, the serum IL-2 content significantly increased in the marine capsules high and the low dose groups ( P<0. 01, P<0. 05). The in vitro growth of tumor (P<0. 001, P<0. 05) was inhibited with the inhibition rate of 41. 56% and 27. 78% respectively for the high dose group and the low dose group, and the effects of the high dose group had no significant differences with those of 5-Fu group (P>0. 05). The nuclear staining of tumor cells was lighter and the number of tumor cells decreased in the two marine capsules groups. Conclusion:Marine capsules have certain inhibitory effects on BALB/c human hepatocellular carcinoma cell line SMMC-7721 in vitro.

Effect of Dressing and Moxibustion-pretreatment on Local Feet Swelling and Stress Hormone in Hypothalami of Rats with Adjuvant Arthritis at Early and Secondary Stages / 中国康复理论与实践

@#ObjectiveTo observe the effect of dressing and moxibustion-pretreatment on the local joint swelling and stress hormone in hypothalami of rats with adjuvant arthritis (AA) at early and secondary stages.MethodsForty Wistar rats were randomly divided into 5 groups: normal group, early and secondary model groups, early and secondary pre-dressing and moxibustion (PDM) groups. The dressing with Chinese herb and moxibustion was stuck on Dazhui point (GV14) before the AA model established. The effects of dressing and moxibustion-pretreatment on the feet swelling and corticotropin-releasing-hormone (CRH), beta-endorphin (β-EP) and neuropeptide-Y (NPY) in hypothalami were observed.ResultsThe right feet swelling rate at early and secondary stages obviously increased after modeling ( P<0.01), and it became lower in early and secondary PDM groups than in model groups at the same phases ( P<0.05～0.01). The level of hypothalamic CRH was higher after modeling ( P<0.05～0.01), compared with early model group, it had a tendency to going down in early PDM group, moreover, in secondary PDM group the level was similar with the normal group. The level of hypothalamic β-EP increased significantly in early model group ( P<0.01), and lightly changed in secondary model group, it decreased in early PDM group but increased in secondary PDM group, compared with model groups at the same stages ( P<0.05). The level of hypothalamic NPY increased significantly after modeling, and it declined in the secondary PDM group ( P<0.05).ConclusionDressing and moxibustion-pretreatment can relief feet swelling of AA rats, which may be related with its regulative effect on the level of hypothalamic CRH, β-EP and NPY.

Effect of dressing-pretreatment of Chinese Traditional Medicine membranous plaster on forepart inflammation in adjuvant arthritis rats / 中国康复理论与实践

@#ObjectiveTo explore the effect of dressing-pretreatment on forepart inflammation in adjuvant arthritis (AA) rats.MethodsAA model was established by subcutaneous injection of Freund's complete adjuvant into the right hind-paw (0.1 ml/animal). 24 Wistar rats were randomly divided into the control group, model group and dressing-pretreatment groups. In dressing-pretreatment group, "Dazhui" (GV14) was applied by a kind of dressing invented by the researchers. The foot swelling rate, serum concentrations of interleukin-1 (IL-1), IL-6 and tumor necrotic factor-α (TNF-α) were assessed.ResultsCompared with the control group, the foot swelling rate and serum concentrations of IL-1, IL-6 and TNF-α of the model group and dressing-pretreatment group increased significantly (P<0.01); but the foot swelling rate and serum concentrations of IL-1 and IL-6 in dressing-pretreatment group decreased significantly compared with those of the model group (P<0.05～0.01), and there was a downtrend in TNF-α. HE stain showed soakage of phlogistic cell in cartilage and arthritis membrane, hyperplasia of vein and breakage of cartilage were better in dressing-pretreatment group compared with that in the model group.ConclusionDressing-pretreatment can decrease forepart inflammation and pathological damage of arthritis in AA rats, and can prevent the harm of consequent disease to some extent.