RESUMO
Thirty nine milk handlers from a factory of dairy products in the Province of Buenos Aires were examined for their nasal carriage of S. aureus strains capable of producing toxic-shock-syndrome toxin-1 (TSST-1). In addition, chance samples of handled foods, crude milk and milky fermented derivates (MFD) were studied. Strain isolation was made on Mannitol Salt Agar and on Baird-Parker Agar. Typical colonies were identified by their biochemical properties. Cultures that were found to be S. aureus were selected for analysis of the TSST-1 production. Eight milk handlers (20.5%) were carriers of S. aureus strains. Seven isolates (87.5%) were classified as biotype A (human ecovar) and 1(12.5%) was classified as biotype B (swine and poultry ecovar). Three out of 8 S. aureus biotype A isolates (37.5%), produced TSST-1. Taking into account the number of milk food handlers sampled (39), the carried rate of toxigenic strains was 7.6%. Three S. aureus strains were isolated from crude milk; 1(33.3%) was classified as biotype B and 2(66.6%) as biotype C (cattle and sheep ecovar). Thirteen S. aureus strains were isolated from MDF; 5(38.0%) were classified as biotype A, 1(7.7%) as belonging to biotype B and 7(53.8%) as belonging to biotype C. None of them had the ability to produce TSST-1.
Assuntos
Toxinas Bacterianas , Portador Sadio/epidemiologia , Laticínios , Enterotoxinas/isolamento & purificação , Contaminação de Alimentos , Manipulação de Alimentos , Microbiologia de Alimentos , Infecções Estafilocócicas/transmissão , Staphylococcus aureus/isolamento & purificação , Superantígenos , Animais , Argentina/epidemiologia , Humanos , Leite , Cavidade Nasal/microbiologia , Staphylococcus aureus/metabolismoRESUMO
Thirty nine milk handlers from a factory of dairy products in the Province of Buenos Aires were examined for their nasal carriage of S. aureus strains capable of producing toxic-shock-syndrome toxin-1 (TSST-1). In addition, chance samples of handled foods, crude milk and milky fermented derivates (MFD) were studied. Strain isolation was made on Mannitol Salt Agar and on Baird-Parker Agar. Typical colonies were identified by their biochemical properties. Cultures that were found to be S. aureus were selected for analysis of the TSST-1 production. Eight milk handlers (20.5
) were carriers of S. aureus strains. Seven isolates (87.5
) were classified as biotype A (human ecovar) and 1(12.5
) was classified as biotype B (swine and poultry ecovar). Three out of 8 S. aureus biotype A isolates (37.5
), produced TSST-1. Taking into account the number of milk food handlers sampled (39), the carried rate of toxigenic strains was 7.6
. Three S. aureus strains were isolated from crude milk; 1(33.3
) was classified as biotype B and 2(66.6
) as biotype C (cattle and sheep ecovar). Thirteen S. aureus strains were isolated from MDF; 5(38.0
) were classified as biotype A, 1(7.7
) as belonging to biotype B and 7(53.8
) as belonging to biotype C. None of them had the ability to produce TSST-1.
