Influence of the Flowable Resin Layer on Bond Strength Between Resin Cement and a Universal Adhesive Applied in the Immediate Dentin-sealing Technique.

OBJECTIVES: The present study evaluated the influence of a flowable resin layer on bond strength between resin cement and a universal adhesive applied using an immediate dentin sealing (IDS) technique. METHODS AND MATERIALS: Coronary portions of bovine teeth were randomly divided into six groups (n=15). In the IDS.U group, the exposed dentin was immediately sealed with the Single Bond Universal adhesive (3M ESPE) following the self-etching protocol. In the IDS.UF group, a layer of Filtek Z350 (3M ESPE) flow resin was applied over the universal adhesive. In the DDS (control) group, the dentin was kept "fresh" and delayed dentin sealing was performed. After 24 hours in distilled water at 37°C, dentin surfaces were treated with pumice, phosphoric acid, and the application of the universal adhesive in the IDS.U and IDS. UF groups. The DDS group was treated with pumice and the universal adhesive was applied. The samples received cylinders of resin cement Rely X Ultimate (3M ESPE) made with the aid of starch tubes of 0.96 mm in diameter and 2 mm in length. They were submitted to the microshear bond strength test (µSBS) at 0.5 mm/min, after 24 hours (T1) and 3 months (T2). The fracture areas were evaluated qualitatively using a DSM 300 microscope (KOZO) with 45× magnification and classified as: adhesive, cohesive in cement, cohesive in dentin, or mixed. Samples were analyzed by scanning electron microscopy (SEM). The data were compared statistically between groups using the Kruskal-Wallis test, and intra-groups using the Mann-Whitney test (α=0.05). RESULTS: There were no significant differences between groups for the bond strength values (p>0.05). The IDS.UF group showed higher values at 3 months, when compared to the values of 24 hours (p<0.001). All groups showed a predominance of adhesive fracture (86.7% to 100%). SEM showed dentinal tubules exposed in the IDS.U and DDS groups; in the IDS.UF group, the tubules were completely sealed. CONCLUSIONS: The flow resin can be used on the adhesive when using the IDS technique because it increased the bond strength values after 3 months and promoted effective sealing of the dentinal tubules.

Relaxant effect of a metal-based drug in human corpora cavernosa and its mechanism of action.

We studied the mechanisms involved in the human corpora cavernosa (HCC) relaxation induced by a new metal-based nitric oxide (NO) donor, the ruthenium complex cis-[Ru(bpy)2Imn(NO)](+3) (FOR0811). FOR0811 produced relaxation in phenylephrine (PE)-precontracted HCC with a maximal response that achieved 112.9 ± 10.6%. There was no difference between the maximal relaxation induced by FOR0811 when compared with sodium nitroprusside (SNP) (106.8 ± 7.3%), BAY41-2272 (107.6 ± 4.1%) or vardenafil (103.4 ± 3.8%), however, FOR0811 was less potent than SNP and vardenafil. L-N(G)-nitroarginine methyl ester (L-NAME), a NO synthase inhibitor, had no effect in the concentration-response curve elicited by FOR0811. 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), a heme-site inhibitor of soluble guanylyl cyclase (sGC) was able to either block or reverse the relaxation induced by FOR0811. On the other hand, the relaxation induced by FOR0811 was not affected by glibenclamide, a blocker of ATP-sensitive potassium channels. FOR0811 (10 µM) was able to increase cyclic guanosine monophosphate (cGMP) levels in corpora cavernosa strips. FOR0811 completely relaxes HCC by a sGC-cGMP-dependent mechanism and can be a lead compound in the development of new stable NO donors.

Dinâmica da poluição fecal nas águas do Córrego Rico, manancial de abastecimento da cidade de Jaboticabal-SP / Fecal pollution dynamics of corrego rico stream and supply water of Jaboticabal city- SP

A qualidade da água in natura é de grande importância nos sistemas de tratamento de águas superficiais, pois uma vez que haja falhas no tratamento pode haver o risco de produzir água contaminada tornando-se prejudicial à saúde dos consumidores. O objetivo do presente trabalho foi conhecer a dinâmica da poluição fecal, durante 24 horas, na água do manancial de abastecimento público da cidade de Jaboticabal, SP. Para isso foram colhidas amostras da água do manancial, com intervalos de 2 horas, durante 24 horas, e foram determinados os Números Mais Prováveis de coliformes totais, Escherichia coli e clostrídios sulfito redutores e as concentrações de amônia nos períodos de chuva e estiagem. Conclui-se que em alguns períodos a água do Córrego Rico não poderia ser utilizada para o abastecimento da população por tratamento convencional, pois com as incidentes chuvas, principalmente no período vespertino, o córrego apresenta contaminação microbiológica acima do limite para sua classe e pode representar risco à saude da população. Os resultados obtidos podem nortear ações visando promover a qualidade da água fornecida à população no sentido de prevenir as enfermidades de veiculação hídrica(AU)

The quality of the supply water source is of great importance to surface water treatment systems, since any kind of treatment failure may send contaminated water that can be harmful to consumer health. The present work aims to monitor fecal pollution dynamics of water from the Corrego Rico stream, as well as from the supply source of Jaboticabal city, SP, during 24 hours. Water samples were collected every 2 hours, during 24 hours and the following variables were determined: Most Probable Numbers of total coliforms, Escherichia coli and sulfite reducing Clostridia, as well as ammonia concentrations during the rainy and dry seasons. It was concluded that during some of the periods, the water from Corrego Rico stream cannot undergo conventional water treatment and be supplied to the population. The incident rains, mainly during the evening, brought water contamination level above the limit allowed for its class. This microbiological contamination may pose a health threat to the population. The results of this study can help to devise a strategy to improve the quality of the water supplied to the population and to prevent waterborne diseases.(AU)

FECAL POLLUTION DYNAMICS OF CORREGO RICO STREAM AND SUPPLY WATER OF JABOTICABAL CITY- SP / Dinâmica da poluição fecal nas águas do Córrego Rico, manancial de abastecimento da cidade de Jaboticabal-SP.

 A qualidade da água in natura é de grande importância nos sistemas de tratamento de águas superficiais, pois uma vez que haja falhas no tratamento pode haver o risco de produzir água contaminada tornando-se prejudicial à saúde dos consumidores. O objetivo do presente trabalho foi conhecer a dinâmica  da poluição fecal, durante 24 horas, na água do manancial de abastecimento público da cidade de Jaboticabal, SP. Para isso foram colhidas amostras da água do manancial, com intervalos de 2 horas, durante 24 horas,  e foram determinados os Números Mais Prováveis de coliformes totais, Escherichia coli e clostrídios sulfito redutores e as concentrações de amônia nos períodos de chuva e estiagem. Conclui-se que em alguns períodos a água do Córrego Rico não poderia ser utilizada para o abastecimento da população por tratamento convencional, pois com as incidentes chuvas, principalmente no período vespertino, o córrego apresenta contaminação microbiológica acima do limite para sua classe e pode representar risco à sau

Dinâmica da poluição fecal nas águas do Córrego Rico, manancial de abastecimento da cidade de Jaboticabal-SP / Fecal pollution dynamics of corrego rico stream and supply water of Jaboticabal city- SP

A qualidade da água in natura é de grande importância nos sistemas de tratamento de águas superficiais, pois uma vez que haja falhas no tratamento pode haver o risco de produzir água contaminada tornando-se prejudicial à saúde dos consumidores. O objetivo do presente trabalho foi conhecer a dinâmica da poluição fecal, durante 24 horas, na água do manancial de abastecimento público da cidade de Jaboticabal, SP. Para isso foram colhidas amostras da água do manancial, com intervalos de 2 horas, durante 24 horas, e foram determinados os Números Mais Prováveis de coliformes totais, Escherichia coli e clostrídios sulfito redutores e as concentrações de amônia nos períodos de chuva e estiagem. Conclui-se que em alguns períodos a água do Córrego Rico não poderia ser utilizada para o abastecimento da população por tratamento convencional, pois com as incidentes chuvas, principalmente no período vespertino, o córrego apresenta contaminação microbiológica acima do limite para sua classe e pode representar risco à saude da população. Os resultados obtidos podem nortear ações visando promover a qualidade da água fornecida à população no sentido de prevenir as enfermidades de veiculação hídrica

The quality of the supply water source is of great importance to surface water treatment systems, since any kind of treatment failure may send contaminated water that can be harmful to consumer health. The present work aims to monitor fecal pollution dynamics of water from the Corrego Rico stream, as well as from the supply source of Jaboticabal city, SP, during 24 hours. Water samples were collected every 2 hours, during 24 hours and the following variables were determined: Most Probable Numbers of total coliforms, Escherichia coli and sulfite reducing Clostridia, as well as ammonia concentrations during the rainy and dry seasons. It was concluded that during some of the periods, the water from Corrego Rico stream cannot undergo conventional water treatment and be supplied to the population. The incident rains, mainly during the evening, brought water contamination level above the limit allowed for its class. This microbiological contamination may pose a health threat to the population. The results of this study can help to devise a strategy to improve the quality of the water supplied to the population and to prevent waterborne diseases.

FECAL POLLUTION DYNAMICS OF CORREGO RICO STREAM AND SUPPLY WATER OF JABOTICABAL CITY- SP / Dinâmica da poluição fecal nas águas do Córrego Rico, manancial de abastecimento da cidade de Jaboticabal-SP

 A qualidade da água in natura é de grande importância nos sistemas de tratamento de águas superficiais, pois uma vez que haja falhas no tratamento pode haver o risco de produzir água contaminada tornando-se prejudicial à saúde dos consumidores. O objetivo do presente trabalho foi conhecer a dinâmica  da poluição fecal, durante 24 horas, na água do manancial de abastecimento público da cidade de Jaboticabal, SP. Para isso foram colhidas amostras da água do manancial, com intervalos de 2 horas, durante 24 horas,  e foram determinados os Números Mais Prováveis de coliformes totais, Escherichia coli e clostrídios sulfito redutores e as concentrações de amônia nos períodos de chuva e estiagem. Conclui-se que em alguns períodos a água do Córrego Rico não poderia ser utilizada para o abastecimento da população por tratamento convencional, pois com as incidentes chuvas, principalmente no período vespertino, o córrego apresenta contaminação microbiológica acima do limite para sua classe e pode representar risco à sau

Effect of ozone application on the resin-dentin microtensile bond strength.

When ozone is used during caries treatment, bond strength can be compromised by the release of oxygen. The use of antioxidant agents neutralizes the free oxygen. The aim of this study was to evaluate the effects of ozone and sodium ascorbate on resin-dentin microtensile bond strength (µTBS). Forty human third molars were divided into four groups: Group 1, not treated with ozone; Group 2, ozone application followed by acid etching; Group 3, acid etching followed by ozone application; and Group 4, ozone and application of sodium ascorbate. Bonded beams (1.0 mm(2)) were tested under tension (0.5 mm min(-1)). The µTBS values were analyzed using one-way analysis of variance (ANOVA) and the Tukey test (p=0.05). All beams that fractured were analyzed under stereomicroscopy (40×). Group 1 had significantly higher µTBS values than Group 2 or 3. The µTBS values of Groups 1 and 4 were similar and higher than those of Group 2. The use of ozone in Group 2 resulted in lower values of µTBS in all conditions evaluated. The predominant failure mode was adhesive. The application of ozone decreased the µTBS of the dentin-composite resin interface. These values were reversed when compared with Groups 1 and 2 when sodium ascorbate was used.

Características da água de consumo animal na área rural da microbacia do Córrego Rico, Jaboticabal, SP / Characteristics of animal drinking water in rural area of Córrego Rico microbasin, Jaboticabal, SP

A qualidade da água oferecida aos animais é um fator importante que deve ser observado na produção animal, pois a água pode veicular organismos patogênicos e substâncias tóxicas prejudiciais à saúde de rebanhos. O objetivo desse trabalho foi caracterizar a qualidade microbiológica e fisico-química da água de fontes de abastecimento e do ponto de consumo animal quanto a potabilidade animal em propriedades rurais da Microbacia Hidrográfica do Córrego Rico, Jaboticabal, SP. Para isso, as análises microbiológicas realizadas foram: determinações dos números mais prováveis de Escherichia coli e enterococos e contagem de microrganismos heterotróficos mesófilos. As análises físico-químicas realizadas foram: determinações do pH, cor e turbidez e concentrações de nitrato e amônia. As colheitas de amostras de água ocorreram na estação de seca e chuva, a fim de se verificar a existência de influência da estação na qualidade da água. Os resultados indicaram que a maioria das fontes era representada por poços, não havia tratamento da água antes de ser oferecida aos animais e os bebedouros não eram limpos com frequência, prejudicando a qualidade da água. Altas porcentagens de amostras de água das fontes e dos bebedouros estavam impróprias para consumo animal, segundo os padrões microbiológicos, nas duas estações. A qualidade físico-química da água nos dois pontos de colheita não estava comprometida de modo de interferir na saúde dos animais. Conclui-se que a água oferecida aos animais necessita de tratamento para garantir sua qualidade, de modo que reduza os riscos de veiculação de doenças.

The quality of animal drinking water is an important factor that must be observed in animal production, once water may carry pathogenic organisms and toxic substances harmful to herds's health. The aim of this study was to characterize the microbiological and physical-chemical water quality of supply sources and water in the point of animal consumption in rural properties in Córrego Rico Microbasin, Jaboticabal, SP. For this, the microbiological tests were performed to determine the most probable numbers of Escherichia coli and enterococci and heterotrophic mesophilic microbial counts. Physico-chemical measurements were: pH, color, turbidity and concentrations of nitrate and ammonia. The collection of water samples occurred in the dry and rainy seasons, to verify the influence of season on water quality. Results indicated that most water sources were represented by wells, no water treatment was realized before water being ofered to the animals and the water troughs were not cleaned frequently, resulting in poor quality of water. High percentages of water samples from sources and water troughs were unfit for animal consumption, according to the microbiological Standards, at the two seasons. pH was the main variable that compromised physical and chemical water quality, in the two collection points. We concluded that animal drinking water requires care to ensure their quality, and then reduces the risk diseases' transmission.

DINÂMICA DA CONTAMINAÇÃO POR INDICADORES BACTERIANOS E EFEITO DA CLORAÇÃO EM TRÊS MANEJOS DA ÁGUA DE DESSEDENTAÇÃO DE BEZERRAS.

O objetivo desse trabalho foi determinar a dinâmica da contaminação por indicadores bacterianos e verificar o efeito do uso do cloro na água de dessedentação de bezerras. Para isso, foram analisadas amostras de água não clorada e clorada dos bebedouros de bezerras mantidas em abrigos móveis individuais em local aberto (manejo 1) e em baias individuais em bezerreiro (manejos 2 e 3). A água permaneceu nos bebedouros à disposição dos animais por 24, 17 e 7 horas nos manejos 1, 2 e 3, respectivamente. A quantificação de enterococos, Escherichia coli e microrganismos heterotróficos mesófilos, e o consumo de cloro foram realizados nas amostras de água. Nos três manejos, nas águas não cloradas e cloradas, ocorreu aumento na contaminação bacteriana para os indicadores bacteriológicos estudados durante a permanência da água nos bebedouros. A utilização do cloro foi fundamental para a melhoria da qualidade da água e a utilização de água clorada por um tempo de permanência de no máximo 7 horas nos bebedouros foi o melhor manejo para garantir a qualidade microbiológica da água.PALAVRAS-CHAVE: Bebedouro. Bovino. Cloro. Microrganismos.

CARACTERÍSTICAS DA ÁGUA DE CONSUMO ANIMAL NA ÁREA RURAL DA MICROBACIA DO CÓRREGO RICO, JABOTICABAL, SP

A qualidade da água oferecida aos animais é um fator importante que deve ser observado na produção animal, pois a água pode veicular organismos patogênicos e substâncias tóxicas prejudiciais à saúde de rebanhos. O objetivo desse trabalho foi caracterizar a qualidade microbiológica e físico-química da água de fontes de abastecimento e do ponto de consumo animal quanto a potabilidade animal em propriedades rurais da Microbacia Hidrográfica do Córrego Rico, Jaboticabal, SP. Para isso, as análises microbiológicas realizadas foram: determinações dos números mais prováveis de Escherichia coli e enterococos e contagem de microrganismos heterotróficos mesófilos. As análises físico-químicas realizadas foram: determinações do pH, cor e turbidez e concentrações de nitrato e amônia. As colheitas de amostras de água ocorreram na estação de seca e chuva, a fim de se verificar a existência de influência da estação na qualidade da água. Os resultados indicaram que a maioria das fontes era representada por poços, não havia tratamento da água antes de ser oferecida aos animais e os bebedouros não eram limpos com frequência, prejudicando a qualidade da água. Altas porcentagens de amostras de água das fontes e dos bebedouros estavam impróprias para consumo animal, segundo os padrões microbiológicos, nas duas estações. A qualidade físico-química da água nos dois pontos de colheita não estava compro

Dinâmica da contaminação por indicadores bacterianos e efeito da cloração em três manejos da água de dessedentação de bezerras / Contamination dynamic of bacteriological indicators and chlorination effect in three managements of drinking water for heifers

O objetivo desse trabalho foi determinar a dinâmica da contaminação por indicadores bacterianos e verificar o efeito do uso do cloro na água de dessedentação de bezerras. Para isso, foram analisadas amostras de água não clorada e clorada dos bebedouros de bezerras mantidas em abrigos móveis individuais em local aberto (manejo 1) e em baias individuais em bezerreiro (manejos 2 e 3). A água permaneceu nos bebedouros à disposição dos animais por 24, 17 e 7 horas nos manejos 1, 2 e 3, respectivamente. A quantificação de enterococos, Escherichia coli e microrganismos heterotróficos mesófilos, e o consumo de cloro foram realizados nas amostras de água. Nos três manejos, nas águas não cloradas cloradas, ocorreu aumento na contaminação bacteriana para os indicadores bacteriológicos estudados durante a permanência da água nos bebedouros. A utilização do cloro foi fundamental para a melhoria da qualidade da água e a utilização de água clorada por um tempo de permanência de no máximo 7 horas nos bebedouros foi o melhor manejo para garantir a qualidade microbiológica da água.

The objective of this study was to determine the contamination dynamic of bacteriological indicators and verify the effect of chlorination in heifers' drinking water. For this, non-chlorinated and chlorinated water samples from water troughs of heifers kept in individual mobile shelters (tropical shelter) (management 1) and in individual stall in covered place (managements 2 and 3) were analyzed. The water remained in the water troughs during 24, 17 and 7 hours in managements 1, 2 and 3, respectively. Enterococcus, Escherichia coli, mesophilic heterotrophic microorganisms and chlorine consumption were determined in the water samples. In the three managements, in non-chlorinated and chlorinated water, was verified increase in bacterial contamination of bacteriological indicators during the permanence of the water in the water troughs. The use of chlorine was important for maintenance of the quality of the water and the use of chlorinated water in the maximum of 7 hours in the water troughs was the better management to guarantee the microbiological quality of water.

Dosimetria proposta para o tratamento por ultra-som: uma revisão de literatura / Dosimetry propost for the treated for ultrasound: a literature of the review

Um dos equipamentos termoterapêuticos utilizados para a produção do calor é o que utiliza princípios de ultra-som (US) produzindo calor profundo pela propagação das suas ondas mecânicas, que são essencialmente as mesmas das ondas sonoras, mas com uma frequência mais alta. O US é produzido por uma corrente alternada que flui por um cristal piezoelétrico, alojado em um transdutor. Os efeitos do US dependem de muitos fatores físicos e biológicos, tais como a iontensidade, o tempo de exposição, a estrutura espacial e temporal do campo ultra-sônico e o estado fisiológico do local a ser tratado. Este grande número de variáveis complica a compreensão exata do seu mecanismo de ação na interação com os tecidos biológicos. Os efeitos do US vem sendo investigados e descritos de maneira empírica através dos tempos e as opinio~es sobre as dosagens utilizadas para o tratamento diferem significativamente, existindo poucas evidências dos seus efeitos clínicos. Sendo assim, este trabalho tem como objetivo apontar, mediante uma revisão bibliográfica, se existem parâmetros físicos e biológicos para determinaçãoi das doses de US, quando utilizado para fins terapêuticos

Clinical evaluation of two "packable" posterior composite resins: two-year results.

The purpose of this study was to evaluate the clinical performance of two "packable" posterior composites: Prodigy Condensable/Optibond Solo-Kerr (PC-OS) and Definite/Etch & Prime-Degussa (D-EP). Thirty-six patients participated in this study. A total of 78 restorations (40 with D-EP and 38 with PC-OS) were made. Each patient received at least two restorations (one of each studied material). The materials were handled according to the manufacturer's instructions. The occlusal adjustments were made at the placement visit. The restorations were finished and polished after 1 week. They were evaluated at baseline, and after 1 year and 2 years by two independent evaluators using the USPHS criteria. Colored slides were made of all the restorations. After 2 years, 34 patients and 74 restorations (38 with D-EP and 36 with PC-OS) were available for evaluation. A total of 50% of PC-OS restorations received A criterion and 50% received B criterion (2.8% color, 11.1% marginal staining, 27.8% superficial staining, 2.8% anatomic form and 5.6% marginal adaptation). For D-EP, 60.5% of restorations received A criterion and 39.5% received B criterion (2.6% color, 5.3% marginal staining, 10.5% superficial staining, 7.9% anatomic form and 13.2% marginal adaptation). The C criterion was observed only for marginal adaptation with D-EP (2 restorations-5.3%). The obtained data were tabulated and statistically analyzed using the Fisher, Chi-square and McNemar tests. After 2 years, PC-OS showed a significant increase in superficial and marginal staining. For D-EP the marginal adaptation and superficial staining became significantly worse than baseline.

Once-daily, low-cost, highly effective Helicobacter pylori treatment to family members of gastric cancer patients.

BACKGROUND: Helicobacter pylori eradication in family members of gastric cancer patients is now widely accepted, although problems related to costs and compliance persist. AIM: To compare the efficacy, tolerability and long-term re-infection rates of two once-daily regimens for the eradication of H. pylori in family members of gastric cancer patients. METHODS: 106 first-degree family members of gastric cancer patients were recruited and submitted to the 13C-urea breath test (UBT) to detect H. pylori. If positive, they were randomly allocated to receive a combination of lanzoprazole 30 mg, clarithromycin OD (extended-release formulation) 500 mg and furazolidone 400 mg, once daily, in the morning, for 7 days (Group A) or the same regimen with only 200 mg furazolidone (Group B). Eradication was confirmed by urea breath test performed 6 weeks after treatment. 13C-urea breath test was repeated at 944 (784-1258) days after treatment in successfully treated participants to look for re-infection. RESULTS: Twenty-five participants were H. pylori negative and two H. pylori-positive individuals refused to sign the informed consent and were excluded. Therefore, 79 participants were studied. Forty participants were allocated to Group A and 39 to Group B. All participants completed treatment. Adverse effects, mostly mild, were observed in 18% of Group A and 18% of Group B (N.S.). The intention-to-treat eradication rate was 87.5% in Group A and 61.5% in Group B (P = 0.006). The mean annual re-infection rate was 3%. CONCLUSIONS: The combination of lanzoprazole 30 mg, one tablet of clarithromycin OD (extended release formulation) 500 mg and furazolidone 400 mg, once daily for 7 days, constitutes an inexpensive, safe and effective alternative for anti-H. pylori therapy in family members of gastric cancer patients.

Clinical evaluation of two "packable" posterior composite resins.

The purpose of this study was to evaluate the clinical performance of two "packable" posterior composites: Prodigy Condensable (P) (Kerr) and Definite (D) (Degussa). Thirty-six patients participated in the study. A total of 78 restorations were made, 40 with D and 38 with P. Each patient received at least two restorations, one of each studied material. The materials were handled according to the manufacturer's instructions. The restorations were finished and polished after 1 week. They were evaluated at baseline and after 1 year by two independent evaluators using the United States Public Health Service (USPHS) criteria. Colored slides were made of all the restorations. After 1 year, 35 patients and 76 restorations (39 with D and 37 with P) were available for evaluation. All restorations received A criteria except the following ones, which received B criteria: color P (one restoration) and D (one restoration), marginal staining P (three restorations) and D (two restorations), surface staining P (nine restorations) and D (three restorations), anatomic form P (one restoration) and D (three restorations), and marginal adaptation P (one restoration) and D (eight restorations). The obtained data were tabulated and statistically analyzed using the Fisher and McNemar tests. After 1 year, P showed a significant increase in superficial staining. For D, the marginal adaptation became significantly worse than baseline and P. The studied materials can be considered acceptable during this evaluation period. Further evaluations are necessary for a better clinical performance analysis.

One-week triple therapy with omeprazole plus azithromycin and secnidazole is ineffective in eradicating "helicobacter pylori"

Background: There is not yet consensus on the most effective treatment for the helicobacter pylori infection, particularly in most developing countries. Azithromycin is a new macrolide and relatively novel agent for H. pylori eradication with an in vitro MIC90 lower than 1 mg/ml.Secnidazole, a nitromidazole that causes fewer side effects than metronidazole, was recenty reported to be used, for the firt time, in the treatment of H. pylori infection. Aim: To evaluate, in a prospective, randomized, single-center study, the association of twodifferent doses of omeprazole, azithromycin and secnidazole in H. pylori eradication. Patients and methods: After informed consent, 55 patients (36m,19F) with duodenal ulcer associated with H. pylori infection were randomized to receive omeprazole 20mg uid (Group A) or 20mg bid (Group B) for sevem days plus azithromycin 500mg uid for six days and secnidazole 2,000mg uid in the first, fourth and seventh day. The H. pylori status was assessed before and 60-90 days posttreatment using urease test, histology and 13C-urea breath test. Statistical analysis was performed by X² test. Results: The two groups had similar demographic characteristics. Fifty-five patients (36M, 19F) were enrolled. Six patients did not show-up for the second visit posttreatment. So, of the 49 evaluable patients, 25por cento (6/24) in Group A and 44por cento(11/25) in Group B wereeradicated, in a per protocol (PP) analysis. Intetion-to-treat (ITT) eradication rates were 21,4por cento (6/28) in Group A and 40.7por cento (11/27) im Group B. The differences betweem ITT and PP analysis from the two groups were not statistically significant. Conclusions: This study shows a very low eradication rate with the two regimens comprising of omeprazole, azithromycin and secnidazole and therefore, should not be recommended for thetreatment of H. pylori infection