The Role of Experience, Perceived Match Importance, and Anxiety on Cortisol Response in an Official Esports Competition.

The aim of the present study was to analyse the neuroendocrine stress response, psychological anxiety response, and perceived match importance (PMI) between expert and non-expert control gamers in an official competitive context. We analyzed, in 25 expert esports players and 20 control participants, modifications in their somatic anxiety, cognitive anxiety, self-confidence, PMI, and cortisol in a League of Legends competition. We found how expert esports players presented higher cortisol concentrations (Z = 155.5; p = 0.03; Cohen's d = -0.66), cognitive anxiety (Z = 99.5; p = 0.001), and PMI (Z = 50.5; p < 0.001) before the competition than non-experts participants. We found a greater statistical weight in the cognitive variables than in the physiological ones. The results obtained suggest that real competitive context and player's expertise were factors associated with an anticipatory stress response. The PMI proved to be a differentiating variable between both groups, highlighting the necessity to include subjective variables that contrast objective measurements.

Differences in Psychoneuroendocrine Stress Responses of High-Level Swimmers Depending on Autocratic and Democratic Coaching Style.

The aim of the present study was to analyse differences in the psychoneuroendocrine stress responses of high-level, young swimmers depending on whether an autocratic and democratic coaching style was applied. Modifications in cortisol and the motivational climate of 18 young swimmers (15.3 ± 1.86 years, 10 females and 8 males) were analysed before and after two training sessions with equivalent training loads but directed by two coaches applying different approaches, i.e., autocratic (A) and democratic (D). The basal testosterone levels of the coaches were also assessed. The basal testosterone concentration was higher in coach A than in coach D; the athletes perceived them as autocratic and democratic, respectively. Swimmers under coach A's instructions showed higher cortisol levels, suggesting higher cortisol production related to coaching style. Furthermore, differences in the motivational climate concerning ego (i.e., athletes comparing their ability with that of other athletes) were observed with coach A, whereas differences in motivational climate concerning the task (i.e., athletes comparing their ability with their own past performance) were observed with coach D. Cognitive variables showed negative perceptions affecting athletes' training experience and performance when they were screamed at or insulted by coach A. There were no gender or age differences in cortisol production or motivational climate. In conclusion, this study suggests that an autocratic coaching style modulates cortisol release in both genders, affecting young elite swimmers' motivational climate and training experience.

3D Hysterosalpingo-contrast sonography: description of the technique and results / Histerosonosalpingografía con contraste 3D: descripción de la técnica y resultados

Objective: To describe the 3-dimensional hysterosalpingo-contrast sonography (3D-HyCoSy) technique and analyze our results with 2 contrast agents: SonoVue® and ExEm Foam®. Material and methods: Cross-sectional study of 160 infertility patients with unknown tubal patency. Results: Bilateral tubal patency was diagnosed in 102/153 (66.7%) patients. A similar proportion of bilateral occlusion was observed with both SonoVue® 5/87 (5.7%) and ExEm foam® 4/66 (6.1%) (p = 0.52). Intrauterine disease was suspected in 33/155 (21.3%) patients: 20% (18/90) with SonoVue® and 23.1% (15/65) with ExEm Foam® (p = 0.644). The visual analog scale (VAS) revealed mild pain (VAS ≤ 4: 86.4% [70/81] with SonoVue® vs. 86.8% [59/68]) with ExEm Foam® (p = 0.951). A pediatric nasogastric probe was easily used to cannulate the cervical os in 128/159 (80.5%) cases. The volume of ExEm foam® used was lower than that of SonoVue® (median: 3 cc vs.20 cc, p < 0.001). Conclusion: 3D-HyCoSy is a reliable, well-tolerated, and effortless tool for the sonographic assessment of sterility. The results were similar with both contrast agents (AU)

Objetivo: describir la técnica y analizar nuestros resultados con histerosonosalpingografía con contraste 3D (HyCoSy-3D) utilizando SonoVue® y Exem Foam®. Material y métodos: estudio retrospectivo de corte transversal en 160 pacientes estériles con permeabilidad tubárica desconocida. Resultados: l 66,7% (102/153) de las pacientes tuvo permeabilidad tubárica bilateral. El diagnóstico de obstrucción tubárica bilateral fue similar utilizando SonoVue® 5/87 (5,7%) y Exem Foam® 4/66 (6,1%), p = 0,52. Diagnosticamos patología intrauterina en 33/155 (21,3%) de las pacientes, 20% (18/90) con SonoVue® vs. 23,1% (15/65) p = 0,644. El dolor percibido resultó leve en la mayoría de los casos (escala visual analógica ≤ 4; 86,4% (70/81) SonoVue® vs. 86,8% (59/68), p = 0,951). La canalización cervical fue sencilla con sonda nasogástrica pediátrica en 128/159 (80,5%). Exem Foam® precisó un menor volumen instilado (mediana: 3 cc vs. 20 cc, p < 0,001). Conclusiones: la HyCoSy-3D es una prueba tolerable, sencilla y rentable para el estudio ecográfico en esterilidad. Ambos contrastes mostraron similares resultados (AU)

Impacto de la linfadenectomía retroperitoneal sistemática en el cáncer de ovario avanzado sin ganglios clínicamente sospechosos / Impact of systematic retroperitoneal lymphadenectomy in advanced ovarian cancer without clinically suspect lymph nodes

Objetivo: investigar el impacto de la linfadenectomía pélvica y paraaórtica sistemática durante la cirugía de citorreducción primaria o la cirugía de intervalo, en pacientes con cáncer de ovario avanzado sin ganglios clínicamente sospechosos. Material y métodos: estudio observacional longitudinal retrospectivo analítico. Se revisaron un total de 42 pacientes diagnosticadas de cáncer de ovario avanzado en el Hospital Universitario Puerta de Hierro entre junio de 2009 y abril de 2016, a las que se practicó una citorreducción primaria o una cirugía de intervalo completa (R0) u óptima (R < 1) y que no presentaban ganglios sospechosos y/o agrandados en el estudio preoperatorio o durante la exploración quirúrgica. Se estimó la supervivencia libre de progresión, la supervivencia global y el patrón de recurrencia en el grupo con linfadenectomía pélvica y paraaórtica sistemática y en el grupo sin linfadenectomía pélvica y paraaórtica sistemática. Resultados: de las 42 pacientes incluidas en el estudio, en 31 se realizó una linfadenectomía pélvica y paraaórtica sistemática y en 11 no. La mediana de seguimiento fue de 32,4 meses, diagnosticándose una recurrencia en el 76,2% de las pacientes y fallecimiento en el 45,2%. No se encontraron diferencias estadísticamente significativas en la supervivencia libre de progresión, la supervivencia global ni en el patrón de recaída entre el grupo de pacientes con linfadenectomía pélvica y paraaórtica sistemática y el grupo sin linfadenectomía pélvica y paraaórtica sistemática. Conclusión: no se ha observado que la linfadenectomía pélvica y paraaórtica sistemática en el cáncer de ovario avanzado sin ganglios clínicamente sospechosos se asocie a una mejora en la superviviencia de las pacientes (AU)

Objective: To investigate the impact of systematic pelvic and paraaortic lymphadenectomy during primary cytoreduction surgery or interval debulking surgery in patients with advanced ovarian cancer without clinically suspected lymph nodes. Material and methods: Observational, longitudinal, analytical and retrospective study. A total of 42 patients diagnosed of advanced ovarian cancer without suspected and/or enlarged lymph nodes in the preoperative study or during the surgical exploration and that were submitted a complete (R0) or optimal (R < 1) primary cytoreduction surgery or interval debulking surgery, were reviewed at Puerta de Hierro University Hospital between June 2009 and April 2016. Progression-free survival, overall survival, and recurrence pattern were estimated in the pelvic and paraaortic lymphadenectomy and non-pelvic and paraaortic lymphadenectomy groups. Results: Of the 42 patients included in the study, 31 were submitted to a pelvic and paraaortic lymphadenectomy and 11 were not. The median follow-up was 32.4 months, diagnosing a recurrence in 76.2% of the patients and death in 45.2%. No statistically significant differences were found in progression-free survival, overall survival and recurrence pattern between the group of patients with pelvic and paraaortic lymphadenectomy and non-pelvic and paraaortic lymphadenectomy. Conclusion: It has not been observed that pelvic and paraaortic lymphadenectomy in avanced ovarian cancer without clinically suspected lymph nodes, is associated with an improvement in patients survival (AU)

Cirugía citorreductora en cáncer epitelial de ovario en estadios avanzados / Cytoreductive surgery in advanced epithelial ovarian cancer

Objetivos: analizar los resultados quirúrgicos de citorreducción primaria y de intervalo en cáncer de ovario avanzado en el Hospital Universitario Puerta de Hierro Majadahonda. Material y métodos: las pacientes incluidas en el estudio fueron mujeres tratadas en nuestro centro de carcinoma epitelial de ovario en estadios III-IV entre 2009 y 2014. Resultados: un total de 39 pacientes fueron incluidas en el estudio. En el 41,03% de los casos se realizó cirugía de citorreducción primaria y en el 58,97% cirugía de intervalo. Se encontró afectación del espacio retrohepático en el 20,5% de los casos. La citorreducción completa se pudo realizar en el 82,1% de los casos. Conclusiones: en nuestro centro conseguimos cirugía de citorreducción completa en un alto porcentaje de casos debido a la colaboración de un equipo multidisciplinar, integrado por ginecólogos y cirujanos generales, capaces de abordar la enfermedad visible, tanto en el hemiabdomen inferior como en el superior (AU)

Objective: To analyze surgical results in primary and interval cytoreductive surgery in advanced ovarian cancer in the Puerta de Hierro Majadahonda University Hospital (Madrid, Spain). Material and methods: We included women with stage III-IV epithelial ovarian cancer who underwent surgery in our centre between 2009 and 2014. Results: A total of 39 patients were included in this study. Of these, 41.03% underwent primary cytoreductive surgery and 58.97% underwent interval surgery. We found retrohepatic evidence of tumour in 20.5% of the patients. Complete cytoreductive surgery was feasible in 82.1%. Conclusions: In our centre, a high percentage of complete cytoreductive surgery was achieved due to the cooperation of a multidisciplinary team composed of gynaecologists and gastrointestinal surgeons, able to deal with visible disease, in both the lower and upper abdomen (AU)

Modelo molecular teórico del receptor serotoninérgico 5HT2A acoplado a proteína G / Theoretical molecular model of the G protein-coupled 5HT2A serotonergic receptor / Modelo molecular teórico do receptor serotoninérgico 5-HT2A acoplado à proteína G

Objetivo. Construir un modelo molecular teórico de la estructura terciara del receptor 5HT2A de Homo sapiens a partir de estructurasobtenidas experimentalmente como plantillas. Materiales y métodos. Para la realización del modelo teórico se contempló el protocoloestablecido por Ballesteros y Weinstein para la construcción del receptor acoplado proteína G, por medio de alineamiento de la secuenciade aminoácidos, perfiles de hidrofobicidad, refinamiento de bucles por restricciones espaciales, y minimización de energía con el campode fuerza OPLS_2005. Resultados. El modelo obtenido fue validado por el gráfico de Ramachandran con un 91,7% de aminoácidosdentro de los límites establecidos para ángulos phi y psi, y un RMSD de 0,95 Å con respecto a rodopsina de bovino. Conclusiones. Seobtuvo un modelo teórico validado, útil para realización de estudios de acoplamiento ligando-receptor...

Objective Build a theoretical molecularmodel of the tertiary structure of the Homo sapiens 5HT2A receptor from experimentally obtained structures as templates. Materialsand methods In the construction of the theoretical model we considered the protocol established by Ballesteros and Weinstein for theconstruction of the G-protein coupled receptor, by the alignment of the amino acid sequence, hydrophobicity profiles, refinement ofloops by spatial restrictions and energy minimization with the force field OPLS_2005. Results The resulting model was validated bythe Ramachandran plot with 91.7% of amino acids within the limits set for angles phi and psi and a RMSD of 0.95 Å with respect tobovine rhodopsin. Conclusions We obtained a validated theoretical model useful in studies of ligand-receptor docking...

Objetivo. Construir um modelo molecularteórico da estrutura terciária do receptor 5HT2A de Homo Sapiens, com estruturas obtidas experimentalmente como moldes. Materiaise métodos. Para a elaboração do modelo teórico se utilizou o protocolo estabelecido por Ballesteros e Weinstein para a construção doreceptor acoplado à proteína G, por intermédio de alinhamento da seqüência de aminoácidos, perfis de hidrofobicidade, refinamento debucles por restrições espaciais e minimização da energia com o campo de força OPLS_2005. Resultados. O modelo obtido foi validadopelo gráfico de Ramachandran com 91,7% dos aminoácidos dentro dos limites estabelecidos para os ângulos phi e psi, e um RMSD de0,95 Å com respeito à rodopsina de bovino. Conclusões. Obteve-se um modelo teórico validado, útil para a realização de estudos deacoplamento ligante-receptor...

Growth capacity of thermotolerant campylobacters in culture media supplemented with pig and cow blood

In this work 60 thermotolerant Campylobacter strains (37 C. jejuni and 23 C. coli) isolated from the cows, pigs, chickens and ducks (15 strains of each type of animal) were used to establish their growth capacity on media containing cow or swine blood as potential substitutes of sheep or horse blood. The growth capacity was assessed by viable counts on cow and swine blood media, using the modified Miles and Misra method. Campylobacter strains showed better growth in the media supplemented with pig or sheep blood than with cow blood. Thus, the use of pig blood could be a supplement for Campylobacter culture medium, when there was no availability of sheep or horse blood.

Na cidade de Iquitos (Região Amazônica do Peru), tanto o sangue de carneiro quanto o de eqüino, ambos recomendados como suplemento nos meios de cultura para Campylobacter, são difíceis de encontrar. No entanto, sangues de bovino e de suíno são de fácil disponibilidade. Por esta razão, 60 amostras de Campylobacter termotolerantes (37 C. jejuni e 23 C. coli) isoladas de bovinos, suínos, frangos e patos (15 amostras de cada animal) foram utilizadas para estabelecer sua capacidade de crescimento em meios de cultura contendo sangue de bovino ou de suíno como potenciais substitutos do sangue de carneiro ou de eqüino. A capacidade de crescimento foi estabelecida através da contagem de células viáveis utilizando o método de Miles e Misra modificado. Todas as amostras de Campylobacter mostraram melhor crescimento em meios suplementados com sangue de eqüino ou de suíno. Estes resultados permitem propor o uso de sangue de suíno como suplementos em meios de cultura para Campylobacter.