Whey filtration: a review of products, application, and pretreatment with transglutaminase enzyme.

In the cheese industry, whey, which is rich in lactose and proteins, is underutilized, causing adverse environmental impacts. The fractionation of its components, typically carried out through filtration membranes, faces operational challenges such as membrane fouling, significant protein loss during the process, and extended operating times. These challenges require attention and specific methods for optimization and to increase efficiency. A promising strategy to enhance industry efficiency and sustainability is the use of enzymatic pre-treatment with the enzyme transglutaminase (TGase). This enzyme plays a crucial role in protein modification, catalyzing covalent cross-links between lysine and glutamine residues, increasing the molecular weight of proteins, facilitating their retention on membranes, and contributing to the improvement of the quality of the final products. The aim of this study is to review the application of the enzyme TGase as a pretreatment in whey protein filtration. The scope involves assessing the enzyme's impact on whey protein properties and its relationship with process performance. It also aims to identify both the optimization of operational parameters and the enhancement of product characteristics. This study demonstrates that the application of TGase leads to improved performance in protein concentration, lactose permeation, and permeate flux rate during the filtration process. It also has the capacity to enhance protein solubility, viscosity, thermal stability, and protein gelation in whey. In this context, it is relevant for enhancing the characteristics of whey, thereby contributing to the production of higher quality final products in the food industry. © 2023 Society of Chemical Industry.

Whey-based polymeric films for food packaging applications: a review of recent trends.

The food industry is always looking for new strategies to extend the shelf life of food. In recent years, the focus has been on edible films and coatings. These play an essential role in the quality, safety, transport, storage, and display of a wide variety of fresh and processed foods and contribute to environmental sustainability. In this sense, this study aimed to carry out a bibliometric analysis and literature review on the production of whey-based films for application in food packaging. Whey-based films have different characteristics when compared to other biopolymers, such as antimicrobial and immunomodulatory capacity. A wide variety of compounds were found that can be incorporated into whey films, aiming to overcome their limitations related to high solubility and low mechanical properties. These compounds range from plasticizing agents, secondary biomacromolecules added to balance the polymer matrix (gelatin, starch, chitosan), and bioactive agents (essential oils, pigments extracted from plants, and other antimicrobial agents). The most cited foods as application matrix were meat (fish, chicken, ham, and beef), in addition to different types of cheese. Edible and biodegradable films have the potential to replace synthetic polymers, combining social, environmental, and economic aspects. The biggest challenge on a large scale is the stability of physical, chemical, and biological properties during application. © 2022 Society of Chemical Industry.

Greek vs traditional yogurts: Sensory and physicochemical comparison

ABSTRACT Greek yogurt (GY) has gained popularity in recent years for its marked texture, taste, and nutritional characteristics compared to traditional yogurt (TY). The objective of this work was to analyze the physicochemical, sensory, and lipid profile of GY and TY with blueberry flavor, both manufactured by a local industry in the state of Rio Grande do Sul, Brazil. Protein and lipid content, as well as humidity, ash, and fatty acid profile were quantified and a sensory evaluation was completed using the affective method. The physicochemical results showed 1.5% and 2.3% more proteins and lipids, respectively, for GY compared to TY. The humidity in TY was 10% lower than in GY. Eighteen types of polyunsaturated, saturated, monounsaturated fatty acids were identified, with a high proportion of C14, C16, and C18. Sensory analysis showed a preference for GY over TY (64% versus 36%, p0.05). Both the protein and lipid content, associated with creaminess, likely influence better acceptance of GY.

RESUMEN El yogur griego (YG) ha ganado popularidad durante los últimos años por su marcada textura, sabor y características nutricionales en comparación con el yogur tradicional (YT). El objetivo de este trabajo fue analizar el perfil fisicoquímico, sensorial y lipídico de YG y YT con sabor a arándano, ambos fabricados por una industria ubicada en el estado de Rio Grande do Sul, Brasil. Fueron cuantificados el contenido de proteínas, lípidos, humedad y cenizas, así como también el perfil de ácidos grasos y la evaluación sensorial por método afectivo. Los resultados fisicoquímicos mostraron que YG contiene 1,5% y 2,3% más de proteínas y lípidos, respectivamente, en comparación con YT (p0,05) en relación a la aceptación de los atributos color, olor, sabor y acidez. Los atributos cuerpo, apariencia y textura presentaron mejores scores de aceptación para el YG. Tanto el contenido de proteínas y lípidos, asociados a la cremosidad, probablemente hayan influenciado una mejor aceptación del YG.

In vitro test to evaluate survival in the gastrointestinal tract of commercial probiotics.

The search for functional foods grows constantly, and in this demand, the supply of industries that seek to produce and sell supplements also grows, as is the case of probiotics freely sold in pharmacies and supermarkets. Given a large number of foods with probiotic appeal and supplements sold without the need for a nutritional or medical prescription, this study came up to evaluate the viability of commercial probiotic cells, through in vitro gastrointestinal simulation and analyzing the information present in their labeling. Eleven commercial probiotic samples were analyzed, and viable cell counts were performed before and after in vitro simulation. These products usually use appealing labeling and induce the consumer to purchase these probiotics, which often do not offer the benefits described on the packaging. The results showed that only two samples had the initial concentration indicated on their labeling and four samples offered a concentration of 3 log CFU g-1 in the ileum portion. All samples had a reduction in concentration during the gastrointestinal simulation, which varied from 1 to 4 log CFU g-1, but most do not fulfill the offer of a probiotic supplement, and there should be more inspection and control over the commercialization of this product niche.

Desenvolvimento de embutido cozido elaborado com carne mecanicamente separada de aves e fibras de colágeno / Development of cooked meat product using the mechanically-deboned poultry meat and collagen fibers

O objetivo do estudo foi o desenvolvimento, a caracterização sensorial e a análise econômica da formulação de um embutido cozido elaborado com carne mecanicamente separada (CMS) de aves e adicionado de fibras de colágeno. Foram elaboradas 4 formulações distintas do produto com CMS e concentrações crescentes de fibras de colágeno: T1-0,5%, T2-1,5%, T3-3,0% e T4-4,0%. Após, foi realizado painel sensorial, avaliando atributos de aparência, aroma, sabor, textura e aceitação global. Os atributos aceitação global e sabor não apresentaram diferença, porém textura e aparência demonstraram maior aceitação nas formulações com maior teor de fibras de colágeno, corroborando com os resultados de textura instrumental. Há uma redução significativa no custo do produto a partir de CMS, mas a adição de fibras de colágeno diminuem a diferença.

Brazilian consumers' perception of edible insects / Percepção de consumidores brasileiros aos insetos comestíveis

ABSTRACT: It is estimated that by 2050 the world population will be 9 billion people; and therefore, the need for alternative sources of protein is inevitable, since conventional sources, such as beef, pork and poultry, will not be sufficient to meet the demand of population growth. Food that includes alternative sources of protein, such as insects, is a reality in countries of Latin America, Asia, Australia, Europe and Africa. This research presents the results of an exploratory study that analysed the food profile of 1,619 consumers in the five Brazilian regions (North, Northeast, Midwest, Southeast and South) as well as their perception, motivation and preferred form of edible insects. The data were analysed by cross-tabulation and expressed as frequencies. Our results show that women are more reluctant than men to consume insects. In general, there is a preference for consumption of insects in the form of flour. However, those with more familiarity with this type of consumption prefer the whole insect. Most Brazilian consumers have no opinion about the safety of consuming insects; however, consumers with higher levels of education and familiarity consider it safe.

RESUMO: Estima-se que em 2050 a população mundial contará com cerca de 9 bilhões de pessoas, e, portanto, a necessidade de fontes alternativas de proteína é inevitável, uma vez que as fontes convencionais, como carne de gado, suínos e aves, não serão suficientes para suprir a demanda do crescimento populacional. A alimentação que inclui fontes alternativas de proteína, como os insetos, é uma realidade em países da América Latina, Ásia, Austrália, Europa e África. Esta pesquisa apresenta os resultados de um estudo exploratório que analisou o perfil alimentar de 1.619 consumidores das cinco regiões brasileiras (Norte, Nordeste, Centro-Oeste, Sudeste e Sul) quanto sua percepção, motivação e forma preferencial aos insetos comestíveis. Os dados foram analisados através de frequências cruzadas e expressos em porcentagem. Nossos resultados mostram que mulheres apresentam maior aversão ao consumo de insetos do que homens. Em geral, há preferência em consumir os insetos na forma de farinha, e inteiro para aqueles com mais familiaridade ao consumo de insetos. Majoritariamente, os consumidores brasileiros não tem opinião sobre a segurança em consumir insetos, contudo, em maiores níveis de escolaridade e a familiaridade os consumidores posicionam-se quanto à sua segurança.

Adding palm oil to the diet of sheep alters fatty acids profile on yogurt: Benefits to consumers.

The aim of this study was to modify the fatty acid profile of yogurt from sheep milk by the inclusion of different concentrations of palm oil into their diet. Thus, thirty-six sheep during lactation were separated in four groups with nine animals each, as described below: the group T0 (0%); the group T2 (inclusion of 2% of palm oil); the group T4 (inclusion of 4% of palm oil) and the group T6 (inclusion of 6% of palm oil). After 60 days of the supplementation, milk samples were collected and yogurt was produced, which was evaluated regarding the concentration of saturated fatty acids (SFA), monounsaturated fatty acids (MFA), and polyunsaturated fatty acids (PFA). A significant reduction (p<0.05) in most SFA and a significant increase (p<0.05) on MFA and PFA was observed in the yogurt of sheep supplemented with 4 and 6% of palm oil. Consequently, it is possible to conclude that palm oil supplementation exerts positive effects on yogurt, since it led to the reduction of undesirable fatty acids and increased fatty acids beneficial to human health.

Adding palm oil to the diet of sheep alters fatty acids profile on yogurt: Benefits to consumers

ABSTRACT The aim of this study was to modify the fatty acid profile of yogurt from sheep milk by the inclusion of different concentrations of palm oil into their diet. Thus, thirty-six sheep during lactation were separated in four groups with nine animals each, as described below: the group T0 (0%); the group T2 (inclusion of 2% of palm oil); the group T4 (inclusion of 4% of palm oil) and the group T6 (inclusion of 6% of palm oil). After 60 days of the supplementation, milk samples were collected and yogurt was produced, which was evaluated regarding the concentration of saturated fatty acids (SFA), monounsaturated fatty acids (MFA), and polyunsaturated fatty acids (PFA). A significant reduction (p<0.05) in most SFA and a significant increase (p<0.05) on MFA and PFA was observed in the yogurt of sheep supplemented with 4 and 6% of palm oil. Consequently, it is possible to conclude that palm oil supplementation exerts positive effects on yogurt, since it led to the reduction of undesirable fatty acids and increased fatty acids beneficial to human health.

Evaluation of bromine and iodine content of milk whey proteins combining digestion by microwave-induced combustion and ICP-MS determination.

The bromine and iodine content of whey protein concentrate (WPC), hydrolysate (WPH), and isolate (WPI) was evaluated combining microwave-induced combustion (MIC) digestion with inductively coupled plasma mass spectrometry (ICP-MS) determination. MIC digestion allowed the decomposition of up to 500 mg of samples using diluted NH4OH solution (25 mmol L(-1)) for absorption of analytes, assuring the compatibility with ICP-MS determination. Accuracy was evaluated using milk powder certified reference material (NIST 8435) with good agreements for Br and I (102% and 105%, respectively). For Br and I, the limit of quantification obtained by ICP-MS was 7 and 281 times lower in comparison with ion chromatography determination, respectively. Iodine could be enriched in whey protein production and up to 70% of the tolerable upper intake level was found, thus revealing the need to monitor it in whey proteins. On the other hand, the concentration of Br was below its acceptable daily intake.

Microbiological and physicochemical characterization of the raw milk and the colonial type cheese from the Northwestern Frontier region of Rio Grande do Sul, Brazil / Caracterização microbiológica e físico-química de leite cru e queijo colonial da região Fronteira Noroeste do Rio Grande do Sul, Brasil

This study aimed at evaluating the microbiological and physicochemical characteristics of raw milk and colonial type cheese from Northwestern Frontier region of Rio Grande do Sul, Brazil. For this purpose, the samples were collected in January and July. Microbiological analyses (aerobic mesophilic bacteria, total/thermotolerant coliform, coagulase-positive Staphylococcus, Salmonella spp. and Listeria monocytogenes, lactic acid bacteria) and physicochemical assays (pH, acidity, total solids, protein, fat, aw, moisture, NaCl) were performed. The milk and cheese samples showed low microbiological quality because high counting of aerobic mesophilic bacteria, total/thermotolerant coliform and coagulase-positive Staphylococcus were detected. High counting of lactic acid bacteria was observed. However, neither Salmonella spp. nor Listeria monocytogenes was found. The standard deviations above one (1.0) in the fat, protein, moisture and salt contents indicated that no standard procedure was followed for producing the local cheese. The sample collection period caused differences in the microbiota, total solids of milk and cheese moisture contents, aw and salt. The maturation period did not significantly influenced on the microbial counts, but it provided an increase in protein contents and a decrease in aw value in cheese samples collected in July.

Este estudo avaliou as características microbiológicas e físico-químicas de leite cru e queijo colonial da região Fronteira Noroeste do Rio Grande do Sul, Brasil. Para isto, foram feitas coletas de amostras em janeiro e julho. Análises microbiológicas (bactérias aeróbias mesófilas, coliformes totais e termotolerantes, Staphylococcus coagulase positiva, Salmonella spp. e Listeria monocytogenes, bactérias ácido lácticas) e físico-químicas(pH, acidez, sólidos totais, proteína, lipídeos, Aa, umidade, NaCl) foram realizadas. As amostras de leitee de queijo indicaram baixa qualidade microbiológica, pois houve detecção de altos níveis de bactériasmesófilas, coliformes totais e termotolerantes e Staphylococcus coagulase positiva. Altas contagens de bactérias ácido láticas foram observadas. Entretanto, não foi detectada a presença de Salmonella spp. eListeria monocytogenes. O desvio padrão acima de um (1,0) nos conteúdos de lipídeos, proteínas, umidade e sal indicou que não houve seguimento do procedimento padrão estabelecido na produção local de queijos. O período de coleta de amostras resultou em diferenças nas análises de microbiota, sólidos totais do leitee dos queijos, o teor de umidade, Aa e sal. O período de maturação não causou significativa influênciasobre as contagens microbianas, mas promoveu aumento no conteúdo de proteína e diminuição na Aa dos queijos coletados em julho.

Yacon: benefícios à saúde e aplicações tecnológicas / Yacon: health benefits and technological applications

No início dos anos 90, foi introduzido na cultura Brasileira o plantio da batata yacon, uma raiz tuberosa, que vem despertando grande interesse por parte dos pesquisadores, devido as suas propriedades nutricionais e tecnológicas. Essa raiz caracteriza-se principalmente por ser rica em frutanos e ácidos fenólicos, apresentando efeito prebiótico e antioxidante, respectivamente. Muitos estudos in vivo e in vitro já foram realizados, utilizando este tubérculo e seus derivados, comprovando suas atividades benéficas à saúde, além deter grande potencial na tecnologia do desenvolvimento de novos produtos alimentícios. A presente revisão objetivou agrupar alguns desses estudos, tanto na área da saúde como na da tecnologia de alimentos. O potencial prebiótico, redução do índice glicêmico e a melhora na saúde óssea são os efeitos que se destacam nas pesquisas in vivo. Percebe-se também uma tendência na elaboração de produtos de panificação utilizando-se a farinha de yacon, e os resultados apresentam-se satisfatórios, tanto nas propriedades tecnológicas quanto nas avaliações sensoriais.

In the early nineties was introduced in Brazil the yacon potato planting. This tuberous roots have been attracting interest from researchers due to their nutritional and technological properties.Yacon is its mainly characterized by being rich in fructans and phenolic acids, with prebiotic and antioxidant effects, respectively. Many studies in vivo and in vitro were carried out with yacon and products produced from it, showing its health benefits, besides having great technological potential for developing new food products .This review aimed to outline some of these studies, in both health and food technology. The prebiotic potential, glycemic index reduction and improvement of bone health are the effects that stand out in the in vivo researches. It can be noted a trend for bakery products formulations using yacon flour, and the outcomes were positive for both technological properties as well as for sensory evaluations.

Candida zeylanoides as a new yeast model for lipid metabolism studies: effect of nitrogen sources on fatty acid accumulation.

Lipid homeostasis is well-known in oleaginous yeasts, but there are few non-oleaginous yeast models apart from Saccharomyces cerevisiae. We are proposing the non-oleaginous yeast Candida zeylanoides QU 33 as model. The aim of this study was to investigate the influence of the carbon/nitrogen ratio and the type of nitrogen source upon oil accumulation by this yeast grown on shake flask cultures. The maximum biomass was obtained in yeast extract (2.39 ± 0.19 g/l), followed by peptone (2.24 ± 0.05 g/l), while the highest content of microbial oil (0.35 ± 0.01 g/l) and the maximum lipid yield (15.63%) were achieved with peptone. Oleic acid was the predominant cellular fatty acid in all culture media (>32.23%), followed by linoleic (>15.79%) and palmitic acids (>13.47%). The highest lipid yield using glucose and peptone was obtained at the C/N ratio of 200:1.

Evaluation of the mineral content of infant formulas consumed in Brazil.

The mineral content of 10 commercially available milk-based infant formulas widely consumed in Brazil was investigated. The levels of elements (Ca, Cu, Fe, K, Mg, Mn, Na, and Zn) were determined by inductively coupled plasma optical emission spectrometry after microwave-assisted wet digestion. The results were compared with the producers' label declarations and levels proposed by the Codex Alimentarius and the Brazilian National Health Surveillance Agency. The obtained results were in good agreement with certified reference materials, and the relative standard deviation of the measurements was always below 10%, with the exception of Cu (up to 14%). A high degree of variation between the experimental and producers' data was found for trace elements among different brands, with differences as high as 41 and 68% for Cu and Zn, respectively. The content of minerals in the starting formulas was in accordance with levels established by the Codex Alimentarius, with the exception of 1 brand that had a Zn level that was not in agreement with the Codex minimum value. Among follow-up formulas, 2 brands contained levels of Ca and K that were higher than the maximum established by Brazilian standards, and 1 brand contained less than the minimum value recommended for Zn. The guidance upper levels have not been established in the Codex Alimentarius for follow-up formulas, and the maximum value has been established for only 2 of the analyzed minerals. This lack of a standard could be a problem; as shown in the present study, if the Brazilian standard is used, several formulas could be considered out of specification. Despite the limited number of samples evaluated in this work, the levels of some elements were out of specification and special attention by the Brazilian government on the content of minerals in infant formulas is needed.

Propriedades de pasta de mesclas de farinha integral com farinha refinada usadas na produção de pães / Pasting properties of whole and refined wheat flour blends used for bread production

Na produção de pães integrais de trigo no Brasil, são utilizadas mesclas de farinha integral (FI) com farinha refinada (FR), as quais diferem em sua composição. Assim, o objetivo deste estudo foi investigar as propriedades de pasta de mesclas de FI com FR. Foram analisadas amostras com 100% de FI, 100% de FR (controles) e mesclas de FI com FR nas proporções de 20, 40, 60 e 80%, em equipamento RVA (Rapid Visco Analyzer). A temperatura de pasta das amostras foi semelhante. A viscosidade máxima diminuiu proporcionalmente com o aumento de FI na mescla. Maiores valores de quebra e de viscosidade mínima à temperatura constante foram encontrados em farinhas com pico de viscosidade elevado. Os valores de viscosidade final e de tendência à retrogradação diminuíram à medida que aumentou o teor de FI na mescla. As propriedades de pasta das mesclas de FI com FR devem ser vistas de forma conjunta, pois afetam a produção de pão e o armazenamento. Os resultados mostraram também que o RVA pode ser usado para ajudar a formular mesclas de FI/FR com certas propriedades de pasta.

In whole wheat bread production in Brazil blends of whole flour (WF) with refined flour (RF) are used, which differ in their composition. Thus, the objective of this study was to investigate the pasting properties of WF with RF blends. Samples with 100% of WF, 100% of RF (controls) and blends of WF with RF in the proportions of 20, 40, 60 and 80% were analyzed, in a RVA (Rapid Visco Analyzer) equipment. The samples pasting temperatures were similar. The peak viscosity decreased proportionally with the increase of WF in blends. Higher values of breakdown and holding strength were found in flour with high viscosity peak. Final viscosity and setback values decreased as the content of WF in blends increased. The pasting properties of WF with RF blends must be seen in a combined way, because they affect bread production and storage. The results also showed that RVA can be used to help in formulating WF/RF blends with certain pasting properties.

Efeito do óleo de eucalipto (Corymbia citriodora) no controle do carrapato bovino / Effect of eucalyptus oil (Corymbia citriodora) on the control of cattle ticks

Esta pesquisa foi conduzida com o objetivo de avaliar o efeito in vitro e in vivo do óleo de eucalipto (Corymbia citriodora) sobre o carrapato bovino (Rhipicephalus (Boophilus) microplus). Na experimentação in vitro, foi utilizado o grupo controle negativo e oito concentrações de óleo de eucalipto (0,5; 1; 2; 5; 10; 20; 50; 100%), em fêmeas ingurgitadas de carrapato. A eficácia de controle foi de 0; 30,5; 75,5; 91; 100; 100; 100; 100 e 100%, respectivamente. Para a experimentação in vivo, foram constituídos três grupos (controle negativo; óleo de eucalipto a 3,5% - nível estimado mediante análise de regressão, correspondendo a 95% de eficácia de controle do carrapato da pesquisa in vitro e amitraz a 0,025%), com dezoito vacas da raça Holandesa. Antes (média dos dias -3, -2,-1) e após a aplicação do produto (1, 2, 3, 5, 7, 10, 14, 21dias), foram contadas fêmeas ingurgitadas de carrapato. A eficácia de controle foi de 0; 96,4 e 69%, respectivamente, 21 dias após o tratamento. Na 1ª e na 2ª ordenha após a aplicação dos tratamentos, foram avaliadas variáveis fisiológicas e coletadas amostras de leite para avaliar as propriedades organolépticas no leite e no iogurte (controle negativo x tratamento fitoterápico). O teste de aceitação sensorial do leite e das variáveis fisiológicas avaliadas foram similares entre os tratamentos.

This research was aimed at evaluating in vitro and in vivo effects of eucalyptus (Corymbia citriodora) oil on cattle ticks (Rhipicephalus (Boophilus) microplus). Negative control group and eight concentrations of eucalyptus oil (0.5; 1; 2; 5; 10; 20; 50; 100%), were used on in vitro trials with engorged female ticks. The efficacy of control ticks was 0; 30.5; 75.5; 91; 100; 100; 100; 100 and 100%, respectively. At the in vivo trial eighteen Holstein cows were allocated to three groups (negative control, eucalyptus oil at 3.5% - level estimated by regression analysis, accounting for 95% efficacy of control ticks on in vitro trial and amitraz at 0.025%). Engorged female ticks were counted before (mean of days -3, -2, -1) and after treatment (1, 2, 3, 5, 7, 10, 14, 21 days). The efficacy control tick was 0; 96.4 and 69%, respectively, at 21 days after treatment. On the 1st and 2nd milked after treatments, physiologic variables were evaluate and milk samples were collected to evaluate organoleptic properties in milk and yoghurt (negative control x phytotherapic treatment). Similar results were found with sensorial acceptance test of milk and physiologic variables.

Caracterização do perfil do consumidor de carne na cidade de Santa Maria (RS), quanto à segurança dos alimentos / Characterization of the consumer profile of meat in the city of Santa Maria (RS), and food safety

Com a finalidade de analisar o conhecimento do consumidor frente à segurança e qualidade dos alimentos foram realizadas entrevistas em um local central de grande fluxo de pessoas na cidade de Santa Maria, RS. Os consumidores foram entrevistados utilizando-se um questionário com questões fechadas e os resultados avaliados através de estatística descritiva. Foi observada uma preocupação em relação à higiene, fiscalização e riscos à saúde, ou seja, assuntos relacionados à segurança dos alimentos.

Aceitação sensorial e caracterização de frozen yogurt de leite de cabra com adição de cultura probiótica e prebiótico / Sensorial acceptance and characterization of goat's milk frozen yogurt with addition of probiotic culture and prebiotic

O leite de cabra é recomendado para pessoas intolerantes ao leite de vaca e/ou portadoras de desordens gastrintestinais. frozen yogurt elaborado a partir de iogurte de leite de cabra foi acrescido de 1 por cento de prebiótico (inulina), 0,25 por cento de cultura starter (Streptococcus thermophilus e Lactobacillus bulgaricus) e 0,25 por cento de cultura probiótica (Bifidobacterium animalis e Lactobacillus acidophilus) para verificar seu potencial probiótico (após sete e 120 dias de armazenamento), sua qualidade físico-química e sua aceitação sensorial. Durante a estocagem, houve redução de células viáveis da flora microbiana, principalmente dos probióticos. Ainda assim, o produto apresenta potencialidade probiótica pela soma de B. animalis e L. acidophilus atingir o mínimo desejável até o fim do armazenamento. Os valores de extrato seco total (26,6±0,2 por cento), proteína (3,0±0,2), gordura (2,6±0,1 por cento), pH (5,5±0,01) e ácido láctico (0,2±0,01 por cento) encontram-se semelhantes aos relatados em outros trabalhos para o mesmo produto. A avaliação sensorial foi realizada por 50 provadores não treinados em teste com escala hedônica de sete níveis e intenção de compra. O produto recebeu bons escores para aparência global, cor, aroma e sabor; já textura e sabor residual obtiveram menores índices de aceitação. Na intenção de compra, 61 por cento dos avaliadores declararam que comprariam o produto, contra 39 por cento que não comprariam. Nessas condições, demonstra-se a viabilidade de elaboração de frozen yogurt de leite caprino acrescido de pre- e probióticos.

Goat's milk is recommended for people afflicted with cow milk intolerance and gastro-intestinal disorders. frozen yogurt was elaborated with 1 percent prebiotic (inulin), 0.25 percent starter culture (Streptococcus thermophilus and Lactobacillus bulgaricus) and 0.25 percent probiotic culture (Bifidobacterium animalis and Lactobacillus acidophilus) to verify probiotic potential (after 7 and 120 days of storage), physical-chemical quality and sensorial acceptance. During storage viable cells were reduced, mainly probiotic bacteria. Nevertheless, the product showed probiotic potential because of B. animalis and L. acidophilus sum, which reached the minimum desirable until the end of storage. Values of total dry matter (26.6±0.2 percent), protein (3.0±0.3 percent), fat (2.6±0.1 percent), pH (5.5±0.01) and acid lactic (0.2±0.01 percent) were similar to those descript by other researches for the same product. Sensorial evaluation was realized by 50 untrained panelists, with a 7- point hedonic scale and intention to purchase. The product received good scores for global appearance, color, aroma and taste. Texture and residual taste had lower acceptance rates. The intent to purchase showed that 61 percent of panelists asserted to buy the product against 39 percent, who would not buy it. Accordingly, this research showed the feasibility of elaborating the goat's milk frozen yogurt with addition of pre- and probiotics.

Emprego de soro de leite líquido na elaboração de mortadela / Employment of fluid whey in the elaboration of mortadela

No presente experimento, o objetivo foi verificar a possibilidade de incorporar soro de leite líquido em mortadela. A água empregada na elaboração da mortadela foi substituída nas proporções de 50, 75 e 100 por cento por soro de leite líquido. Foram avaliadas as características sensoriais (cor, odor, sabor e textura), pH, coloração, estabilidade da emulsão da mortadela, assim como a percentagem de gordura e líquido, liberados após o tratamento térmico da emulsão da mortadela. A adição de soro de leite líquido não exerceu influência significativa nas características sensoriais, pH, coloração, estabilidade da emulsão e líquido liberado após o tratamento térmico das mortadelas. A substituição de 75 e 100 por cento da água por soro de leite líquido, na elaboração da mortadela proporcionou menor percentual de gordura liberada após o tratamento térmico. Com base nos resultados obtidos, pode-se dizer que o soro líquido pode ser usado para substituir 100 por cento do gelo usado na elaboração da mortadela, evitando que o soro excedente seja liberado no meio ambiente.

The objective of this paper was to verify the possibility of incorporating fluid whey in mortadela. The water employed in the elaboration of mortadela was replaced in the proportions of 50, 75 and 100 percent by fluid whey. Sensorial characteristics (color, odor, flavor and texture), pH, color, emulsion stability of mortadela were evaluated, as well as the percentage of fat and liquid released after thermal treatment of mortadela emulsion. The addition of fluid whey did not exert significant influence on the sensorial characteristics, pH, color, emulsion stability and liquid released after the thermal treatments in mortadela. Water substitutions of 75 and 100 percent by fluid whey in the elaboration of mortadela promoted a lower percentage of fat released after thermal treatment. Based on the obtained results it is clear that fluid whey can be used to substitute 100 percent of ice used in the elaboration of mortadela, thus avoiding that exceeding fluid whey be released in the environment.

Avaliação das condiçöes higiênico sanitárias de sorvetes tipo italiano (soft), comercializados na cidade de São Leopoldo, RS / Evaluation of hygienic and sanitary conditions of Italian type ice-cream (soft cream), commercialized in the city of São Leopoldo, RS

Teve como objetivo mostrar as condições higiênico-sanitárias de sorvetes de creme tipo italiano (soft), comercializados na cidade de São Leopoldo (RS), processados artesanalmente. Foram analisadas 16 amostras entre novembro de 1999 a janeiro de 2000, em 8 pontos da cidade. Realizaram-se as análises do número mais provável (NMP) de coliformes totais e fecais; confirmação de E. coli; contagem total de aeróbicos mesófilos e Staphylococcus aureus. A metodologia utilizada foi recomendada pela American Public Health Association (APHA, 1992). Os resultados foram comparados com os padrões previstos na legislação (Resolução Normativa nÝ4/78 - CTA). Verificou-se que para a contagem geral de microrganismos aeróbicos estritos e facultativos viáveis 100 por cento das amostras estavam dentro da legislação vigente. Para coliformes totais 56,25 por cento das amostras estão acima do padrão estabelecido pela legislação. Esta mesma porcentagem foi confirmada para coliformes fecais, sendo que este último não está previsto na legislação. As contagens de S.aureus em 12,5 por cento das amostras foram superiores aos valores previstos pela legislação. A presença de coliformes fecais e a confirmação, em algumas amostras, de E.coli, denotam falta de princípios básicos de higiene na fabricação e manipulação de tais sorvetes.