Technical-functional and surface properties of white common bean proteins (Phaseolus vulgaris L.): Effect of pH, protein concentration, and guar gum presence.

Legumes are abundant sources of proteins, and white common bean proteins play an important role in air-water interface properties. This study aims to investigate the technical-functional properties of white common bean protein isolate (BPI) as a function of pH, protein concentration, and guar gum (GG) presence. BPI physicochemical properties were analyzed in terms of solubility, zeta potential, and mean particle diameter at pH ranging from 2 to 9, in addition to water-holding capacity (WHC), oil-holding capacity (OHC), and thermogravimetric analysis. Protein dispersions were evaluated in terms of dynamic, interfacial, and foam-forming properties. BPI showed higher solubility (>80 %) at pH 2 and above 7. Zeta potential and mean diameter ranged from 15.43 to -34.08 mV and from 129.55 to 139.90 nm, respectively. BPI exhibited WHC and OHC of 1.37 and 4.97 g/g, respectively. Thermograms indicated decomposition temperature (295.81 °C) and mass loss (64.73 %). Flow curves indicated pseudoplastic behavior, with higher η100 values observed in treatments containing guar gum. The behavior was predominantly viscous (tg δ > 1) at lower frequencies, at all pH levels, shifting to predominantly elastic at higher frequencies. Equilibrium surface tension (γeq) ranged from 43.87 to 41.95 mN.m-1 and did not decrease with increasing protein concentration under all pH conditions. All treatments exhibited ϕ < 15°, indicating predominantly elastic surface films. Foaming properties were influenced by higher protein concentration and guar gum addition, and the potential formation of protein-polysaccharide complexes favored the kinetic stability of the system.

Development of a Polymeric Membrane Impregnated with Poly-Lactic Acid (PLA) Nanoparticles Loaded with Red Propolis (RP).

The main objectives of this study were to develop and characterize hydrophilic polymeric membranes impregnated with poly-lactic acid (PLA) nanoparticles (NPs) combined with red propolis (RP). Ultrasonic-assisted extraction was used to obtain 30% (w/v) red propolis hydroalcoholic extract (RPE). The NPs (75,000 g mol-1) alone and incorporated with RP (NPRP) were obtained using the solvent emulsification and diffusion technique. Biopolymeric hydrogel membranes (MNPRP) were obtained using carboxymethylcellulose (CMC) and NPRP. Their characterization was performed using thermal analysis, Fourier transform infrared (FTIR), total phenols (TPC) and flavonoids contents (TFC), and antioxidant activity through the radical scavenging assay with 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and Ferric reducing antioxidant power (FRAP). The identification and quantification of significant RP markers were performed through UPLC-DAD. The NPs were evaluated for particle size, polydispersity index, and zeta potential. The TPC for RPE, NPRP, and MNPRP was 240.3 ± 3.4, 191.7 ± 0.3, and 183.4 ± 2.1 mg EGA g-1, while for TFC, the value was 37.8 ± 0.9, 35 ± 3.9, and 26.8 ± 1.9 mg EQ g-1, respectively. Relevant antioxidant activity was also observed by FRAP, with 1400.2 (RPE), 1294.2 (NPRP), and 696.2 µmol Fe2+ g-1 (MNPRP). The primary markers of RP were liquiritigenin, isoliquiritigenin, and formononetin. The particle sizes were 194.1 (NPs) and 361.2 nm (NPRP), with an encapsulation efficiency of 85.4%. Thermal analysis revealed high thermal stability for the PLA, nanoparticles, and membranes. The DSC revealed no interaction between the components. FTIR allowed for characterizing the RPE encapsulation in NPRP and CMC for the MNPRP. The membrane loaded with NPRP, fully characterized, has antioxidant capacity and may have application in the treatment of skin wounds.

Jamelão capsules containing bioactive compounds and its aplication in yoghurt.

BACKGROUND: Jamelão fruit (Syzygium cumini), has recently attracted interest as a functional food for being rich in anthocyanins, which has antioxidant power, attractive color and stability in high acid foods. METHODS: Samples of yoghurt with jamelão capsules obtained through the gelling process with sodium alginate solution and bioactive yoghurt (control + capsules) were evaluated. The samples were evaluated for composition, microbial viability, the stability on the anthocyanic pigments and its antioxidant activity. RESULTS: With the addition of jamelão pulp capsules there was a reduction in the Streptococcus thermophilus count. The addition of capsules in yoghurts were able to increase the amount of phenols and anthocyanins, as well as antioxidant potential at 28 days. The chromatographic analysis showed that process was efficient, being capable of encapsulating a large part of the bioactive compounds present in the pulp. CONCLUSIONS: The addition of jamelão pulp capsules to yoghurts was shown to be a promising and viable alternative, since the bioactive compounds present in the fruit were present in the yoghurt.

Sorvetes: alternativas de estabilizantes naturais e influência da técnica de congelamento / Ice creams: alternatives to natural stabilizers and freezing date influence

O objetivo deste trabalho foi testar diferentes aditivos naturais na formulação do sorvete de maracujá e avaliar a influência do congelamento lento (-18ºC) e rápido (-80ºC). Foram preparadas 3 formulações do sorvete, variando apenas o aditivo (F1: Superliga Neutra, F2: Gelatina e F3: Ágar). Estas amostras foram avaliadas com relação a composição nutricional e propriedades físicas e químicas. O congelamento rápido conferiu as 3 formulações uma uniformidade na velocidade de derretimento (p>0,05). A formulação F2 foi a que apresentou melhor resultado quando avaliado a resistência ao derretimento, overrun e composição centesimal. Embora todas as formulações tenham apresentado resultados positivos, a F2 associado ao ultracongelamento apresentou o melhor perfil de sorvete.(AU)

Enriquecimento proteico de resíduo de frutas através de fermentação semi-sólida utilizando Saccaromyces cerevisae / Protein enrichment of fruit residue through semi-solid fermentation using Saccaromyces cerevisae

Toneladas de resíduos são gerados no processamento de frutos, fazendo parte a casca e sementes. Esses resíduos possuem nutrientes e compostos antioxidantes importantes para o organismo humano, que podem ser fermentados em biorreatores, minimizando o desperdício, gerando uma nova fonte alimentar. Logo, objetivou-se o enriquecimento proteico dos resíduos de frutas, através da fermentação semi-sólida, visando o aumento do teor de proteína e melhorando seu valor nutricional. Os resíduos de maracujá, laranja e goiaba foram misturados com 15% de levedura e foram inseridos em biorreatores tipo bandejas de alumínio a 37 ºC em um período 60 horas, onde foram retiradas amostras em intervalos de 8 horas. Os resíduos foram bem aproveitados, apresentando-se como uma fonte reutilizável no processo de fermentação e com expressivo valor proteico.(AU)

Enriquecimento proteico de resíduo de frutas através de fermentação semi-sólida utilizando Saccaromyces cerevisae / Protein enrichment of fruit residue through semi-solid fermentation using Saccaromyces cerevisae

Toneladas de resíduos são gerados no processamento de frutos, fazendo parte a casca e sementes. Esses resíduos possuem nutrientes e compostos antioxidantes importantes para o organismo humano, que podem ser fermentados em biorreatores, minimizando o desperdício, gerando uma nova fonte alimentar. Logo, objetivou-se o enriquecimento proteico dos resíduos de frutas, através da fermentação semi-sólida, visando o aumento do teor de proteína e melhorando seu valor nutricional. Os resíduos de maracujá, laranja e goiaba foram misturados com 15% de levedura e foram inseridos em biorreatores tipo bandejas de alumínio a 37 ºC em um período 60 horas, onde foram retiradas amostras em intervalos de 8 horas. Os resíduos foram bem aproveitados, apresentando-se como uma fonte reutilizável no processo de fermentação e com expressivo valor proteico.

Sorvetes: alternativas de estabilizantes naturais e influência da técnica de congelamento / Ice creams: alternatives to natural stabilizers and freezing date influence

O objetivo deste trabalho foi testar diferentes aditivos naturais na formulação do sorvete de maracujá e avaliar a influência do congelamento lento (-18ºC) e rápido (-80ºC). Foram preparadas 3 formulações do sorvete, variando apenas o aditivo (F1: Superliga Neutra, F2: Gelatina e F3: Ágar). Estas amostras foram avaliadas com relação a composição nutricional e propriedades físicas e químicas. O congelamento rápido conferiu as 3 formulações uma uniformidade na velocidade de derretimento (p>0,05). A formulação F2 foi a que apresentou melhor resultado quando avaliado a resistência ao derretimento, overrun e composição centesimal. Embora todas as formulações tenham apresentado resultados positivos, a F2 associado ao ultracongelamento apresentou o melhor perfil de sorvete.