Differential expression in ACE2, Ang(1-7) and Mas receptor during progression of liver fibrosis in a rat model / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To investigate the changes in expression of the ACE2/Ang(1-7)/Mas receptor axis' components that occur during progression of liver fibrosis using a rat model system.</p><p><b>METHODS</b>Thirty-six adult male Wistar rats, were randomly assigned to groups of normal control (n = 6; no manipulation) and liver fibrosis (n = 30; given a subcutaneous injection of 40% chronic carbon tetrachloride (CCl4)). At post-injection days 15, 30, 45, 60 and 75, 1 control rat and 6 modeled rats were sacrificed for analysis. Histopathological analysis of liver tissue was performed with hematoxylin-eosin and rapid Masson staining. Protein expression level of Ang(1-7) was determined by enzyme-linked immunosorbent assay, and of ACE2 and Mas receptor was evaluated by immunohistochemistry. Real-time PCR was used to measure the mRNA expression levels of ACE2 and Mas receptor.</p><p><b>RESULTS</b>The expression levels of ACE2, Ang(1-7) and Mas receptor showed a statistically significant upward trend that followed the progression of fibrosis up to post-injection day 60 (P less than 0.01), but the significant increase was not seen from day 60 to day 75.</p><p><b>CONCLUSION</b>Each component of the ACE2/Ang(1-7)/Mas receptor axis shows differential expression during the development of liver fibrosis and may contribute to disease progression.</p>

Color Doppler in differential diagnosis of scrotal mass / 中华男科学杂志

<p><b>OBJECTIVE</b>To evaluate the differential diagnosis of scrotal mass with color Doppler ultrasound.</p><p><b>METHODS</b>Retrospective analysis was made of 21 cases of scrotal mass confirmed both surgically and pathologically in our hospital.</p><p><b>RESULTS</b>Eight of the total number were malignancy of the testis origin, accounting for 38.1% of whole study group and 13 were benign, accounting for 61.9%. Of the 13 benign cases, only 2 were of the testis origin (15.4%) while the other 11 (84.6%) were not.</p><p><b>CONCLUSION</b>Color Doppler ultrasound plays an increasingly important role in the differential diagnosis of scrotal mass.</p>

Mass Culture and Purification of Vero-based Influenza Virus / 中国生物工程杂志

Objective:Establish mass-scale purification technology of cell-derived influenza virus. Methods: A microcarrier-based process was used to produce human influenza virus in serum free-adapted Vero cells. The virus was purified in a sequence of downstream processing steps including inactivation, clarification, anion exchange chromatography, affinity chromatography and size-exclusion chromatography. Results: The recovery of HA reached 102%. 95.3% total protein, including 99.77% host cell protein, and 99.99% host cell DNA were removed during downstream processing. Conclusion: Providing a high effective purification technology for cell-derived influenza virus.