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Objective:To establish a method to determine the content of chlorogenic acid and rutin in nettle to assess the quality of nettle.Methods:An HPLC method was used with the following chromatographic conditions:CAPCELL PAK C18 column (250 mm × 4.6 mm, 5 μm) was used with the mixture of 0.4%phosphoric acid and methanol as the mobile phase with gradient elution , the de-tection wavelength was set at 340 nm, the flow rate was 1.0 ml· min-1 and the sample size was 10μl.Results:Chlorogenic acid with-in the range of 2.360-23.600μg · ml-1 showed a good linear relationship (r=0.999 9), and rutin within the range of 6.208-62.080μg · ml-1 showed a good linear relationship (r=0.999 9).The recovery of rutin and chlorogenic acid was 99.20% and 100.40%with RSD of 1.2%and 1.1%(n=6), respectively.Conclusion:The method is fast, effective, simple, accurate and reproducible , which can be used to quantitatively analyze chlorogenic acid and rutin in nettle .
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0.05), a extreme significantly difference in the laboratory examination results in each group between before and after treatment (P
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Objective With fat-soluble ingredients and water-soluble components as indicators,to investigate the processing technology of wine Gansu Salvia by orthogonal test.Method With fat-soluble ingredients and water-soluble components determined by HPLC,the processing technology of wine Gansu Salvia was optimized by orthogonal test.Result The transfer rate of fat-soluble ingredients with water extraction was 2.4%,with little significance,while it had a significant impact on salvianolic acid B.The best process condition was 10% yellow rice wine,added 20% wine,fried in 80 ℃ for 4 min.Conclusion It was reasonable to choose the best process condition for wine Gansu Salvia.
