Perceived quality of life by patients with immune-mediated inflammatory diseases treated with biological therapies. SACVINFA study.

OBJECTIVES: To evaluate health-related quality of life perceived by patients with the most prevalent immune-mediated inflammatory diseases in Spain: inflammatory bowel disease (IBD), psoriasis (Ps), psoriatic arthritis (AP), rheumatoid arthritis (RA), and spondyloarthropathies (SpAs), and to determine the factors that influence patient quality of life. METHODS: The SACVINFA study (SA=satisfaction, CV=quality of life, IN=immune-mediated, FA=pharmacy) consisted of an observational study conducted in 4 hospitals in the Community of Madrid. A cross-sectional analysis was made for adult patients diagnosed with an immune-mediated inflammatory disease who attended the Pharmacy Service. Quality of life was assessed using the EQ-5D-5L questionnaire (mobility, self-care, usual activities, pain/discomfort, and anxiety/depression) and specific questionnaires: SIBDQ-9, DLQI, PsAQoL, QoL-RA, and ASQoL. RESULTS: A total of 578 patients were analysed (inflammatory bowel disease=25.3%; psoriasis=19.7%; spondyloarthropathies=18.7%; rheumatoid arthritis=18.5%; psoriatic arthritis=17.8%). The mean age (standard deviation) was 49.8 (12.3) years and 50.7% were male. The average score (standard deviation) for the global EQ-5D-5L was 0.771 (0.2) and the mean (standard deviation) visual analogue scale score was 71.5 (20.0). Type of immune-mediated inflammatory diseases was associated with differences in quality of life showing psoriasis and inflammatory bowel disease higher values of EQ5D-5L than psoriatic arthritis, rheumatoid arthritis, and spondyloarthropathies, p<.05 in all comparisons. Patients with RA, IBD, and Ps achieved 70% of the maximum score, while patients with PsA and SpAs did not reach 50% of the maximum possible score. Female gender, a state of moderate/severe disease severity, an older age, and a higher number of previous treatments were correlated with worse quality of life. Conversely, persistence to current treatment correlated with better quality of life. CONCLUSIONS: Patients with immune-mediated inflammatory diseases have markedly affected quality of life, mainly in the pain/discomfort dimension, especially in those immune-mediated inflammatory diseases with a rheumatological component.

Aceite esencial de hojas de Minthostachys mollis [HBK] Griseb. del Ecuador: extracción, composición química, capacidad antioxidante y actividad antimicrobiana / Essential oil of Minthostachys mollis [HBK] Griseb. leaves from Ecuador: extraction, chemical composition, antioxidant capacity and antimicrobial activity

The aim of this study was to optimize by response surface design, the extraction of the leaf essential oil (EO) from Minthostachys mollis [HBK] Griseb., grown in Ecuador, using steam distillation. The factors used were extraction time (XTIE) of 60, 105 and 150 min and plant material/water ratio (XRMA) of 1:3, 1:4 and 1:5. The optimal combination was reached with XRMA 1:5 and XTIE 150 min, obtaining a process yield of 0.67%. The chemical composition of the EO analyzed by GC - MS was determined, where the main compounds were carvacryl acetate (44.01%), carvacrol (16.51%) and menthone (8.20%). The anti oxidant capacity of EO was evaluated using the FRAP and ABTS methodologies, with an IC 50 243.21 µmol Fe 2+ /g and 0.12 mg/mL, respectively. In addition, the antimicrobial activity of EO was found against Pseudomonas aeruginosa , Salmonella enterica , Escherich ia coli and Staphylococcus aureus .

El objetivo del estudio fue optimizar, mediante un diseño de superficie respuesta, la extracción d el aceite esencial (AE) de hojas de Minthostachys mollis [HBK] Griseb. del Ecuador, mediante destilación por arrastre de vapor. Los factores fueron el tiempo de extracción (XTIE) de 60, 105 y 150 min, y relación de material vegetal/ agua destilada (XRMA) d e 1:3, 1:4 y 1:5. La combinación óptima se logró con XTIE 150 min y XRMA 1:5 para un rendimiento de 0,67%. Se determinó la composición química del AE por GC - MS donde los compuestos mayoritarios fueron acetato de carvacrilo (44,01%), carvacrol (16,51%) y me ntona (8,20%). Se evaluó la capacidad antioxidante del AE por las metodologías FRAP y ABTS, con CI 50 de 243,21 µmol Fe 2+ /g y 0,12 mg/mL, respectivamente. Además, se demostró la actividad antimicrobiana contra Pseudomonas aeruginosa , Salmonella enterica , Es cherichia coli y Staphylococcus aureus .

Health-promoting peptides in fermented beverages.

Since ancient times, the consumption of fermented low-alcoholic beverages has enjoyed widespread popularity in various countries, because of their distinct flavors and health benefits. Several studies have demonstrated that light to moderate alcohol consumption is associated with beneficial effects on human health, mainly in cardiovascular disease prevention. Fermented beverages have different non-ethanol components that confer beneficial health effects. These bioactive compounds are mainly peptides that have often been overlooked or poorly explored in numerous fermented beverages. The aim of this review is to provide knowledge and generate interest in the biological activities of peptides that are present and/or released during the fermentation process of widely consumed traditional fermented beverages. Additionally, a brief description of the microorganisms involved in these beverages is provided. Furthermore, this review also explores topics related to the detection, isolation, and identification of peptides, addressing the structure-activity relationships of both antioxidant and angiotensin-converting enzyme inhibitory (ACE-I) activities.

The impact of COVID-19 and other factors on the usage status of the biologic drug therapies for rheumatoid arthritis: A study from Vietnam / El impacto de COVID-19 y otros factores en el estado de uso de las terapias farmacológicas biológicas para la artritis reumatoide: un estudio de Vietnam

Objectives: To describe the status of using biological Disease Modifying Anti Rheumatic Drugs (bDMARDs) to treat rheumatoid arthritis (RA) and related factors. In addition, the study determined the impact of COVID-19 on the usage of bDMARDs. Methods: This is a cross-sectional study and included 219 RA patients over 18 years old. The Kaplan–Meier method and the log-rank test (p<0.05) were used to estimate the retention time and compare between different times. Cox regression analysis was used to determine the factors affecting the retention time of biological drugs (p<0.05). Results: Out of 1967 courses of treatment, there were 149 (7.6%) drug discontinuations, 760 (38.6%) doses extensions and 64 (3.3%) drug switch. Moderate disease level and choosing tumor necrosis factor (TNF) inhibitors initially were associated with retention time of COVID-19. Drug discontinuations and dose extensions increased after COVID-19 emergence. The retention time during COVID-19 was significantly different from that of pre-COVID-19. Gender, type of first-used bDMARD, conventional synthetic DMARDs (csDMARDs) and corticoid usage status, disease activity levels were associated with retention time. Conclusion: The presence of COVID-19 has a significant effect on usage status of the biologic drug. Further longitudinal studies are needed to clarify the relationship between COVID-19 and drug usage as well as related factors.(AU)

Objetivos: Describir el estado del uso de fármacos antirreumáticos modificadores de la enfermedad biológica (bDMARD) para tratar la artritis reumatoide (AR) y los factores relacionados. Además, el estudio determinó el impacto de COVID-19 en el uso de bDMARD. Métodos: Este es un estudio transversal que incluyó a 219 pacientes con AR mayores de 18 años. El método Kaplan-Meier y la prueba Log-rank (p<0,05) se usaron para estimar el tiempo de retención y compararlo entre diferentes tiempos. El análisis de regresión de Cox se utilizó para determinar los factores que afectan el tiempo de retención de los medicamentos biológicos (p<0,05). Resultados: De 1.967 cursos de tratamiento, hubo 149 (7,6%) interrupciones del fármaco, 760 (38,6%) extensiones de dosis y 64 (3,3%) cambios de fármaco. Nivel de enfermedad moderado y elección del factor de necrosis tumoral (TNF) inhibidores inicialmente se asociaron con el tiempo de retención de COVID-19. Las discontinuaciones de los medicamentos y las extensiones de las dosis aumentaron después de la aparición de COVID-19. El tiempo de retención durante COVID-19 fue significativamente diferente del pre-COVID-19. Género, tipo de bDMARD de primer uso, convencional DMARD sintéticos (csDMARDs) y el estado de uso de corticoides, los niveles de actividad de la enfermedad se asociaron con el tiempo de retención. Conclusión: La presencia de COVID-19 tiene un efecto significativo en el estado de uso del medicamento biológico. Se necesitan más estudios longitudinales para aclarar la relación entre COVID-19 y el uso de fármacos, así como los factores relacionados.(AU)

The impact of COVID-19 and other factors on the usage status of the biologic drug therapies for rheumatoid arthritis: A study from Vietnam.

OBJECTIVES: To describe the status of using biological Disease Modifying Anti Rheumatic Drugs (bDMARDs) to treat rheumatoid arthritis (RA) and related factors. In addition, the study determined the impact of COVID-19 on the usage of bDMARDs. METHODS: This is a cross-sectional study and included 219 RA patients over 18 years old. The Kaplan-Meier method and the log-rank test (p<0.05) were used to estimate the retention time and compare between different times. Cox regression analysis was used to determine the factors affecting the retention time of biological drugs (p<0.05). RESULTS: Out of 1967 courses of treatment, there were 149 (7.6%) drug discontinuations, 760 (38.6%) doses extensions and 64 (3.3%) drug switch. Moderate disease level and choosing tumor necrosis factor (TNF) inhibitors initially were associated with retention time of COVID-19. Drug discontinuations and dose extensions increased after COVID-19 emergence. The retention time during COVID-19 was significantly different from that of pre-COVID-19. Gender, type of first-used bDMARD, conventional synthetic DMARDs (csDMARDs) and corticoid usage status, disease activity levels were associated with retention time. CONCLUSION: The presence of COVID-19 has a significant effect on usage status of the biologic drug. Further longitudinal studies are needed to clarify the relationship between COVID-19 and drug usage as well as related factors.

Thematic assessment report on invasive alien species in Brazil: summary for policymakers / Relatório temático sobre espécies exóticas invasoras no Brasil: sumário para tomadores de decisão

Abstract Biological invasions are one of the major threats to biodiversity and good quality of life, resulting from the translocation of species by human action. There are more than 500 alien species currently invading ecosystems in Brazil, particularly plants and fishes, while little is known about invasive microorganisms. Although invasive alien species are present in all ecosystems in the country, most have been recorded in habitats with greater human interference, such as urban and peri-urban areas, farmland, dams, reservoirs, ports, and canals. Historically, the southern and southeastern regions of Brazil have had more invasive alien species, but there has been an increase in the number of invasive alien species in the central-western and northern regions in recent decades. The ornamental trade of plants and fishes as well as the illegal pet trade of wild mammals and reptiles are some of the main pathways for invasive species introduction and spread in Brazil. Breeding and cultivation systems that allow escape to natural areas are a relevant route of species introductions in freshwater ecosystems, while unintentional introductions from shipping and infrastructure are of extreme concern in marine ecosystems. The negative impacts of invasive alien species on the biota mainly include changes in community structure and local decrease in native species richness, mediated by predation, competition, and ecosystem changes. Most negative impacts are recorded for intentionally introduced species, such as fishes and plants, but unintentional introductions have led to impacts on good quality of life, with associated costs and impacts on human health. The management of biological invasions faces challenges that need to be overcome, such as the lack of public knowledge about the impact of invasive alien species, the popular appeal of charismatic invasive species or those used by humans, and the use of controversial control techniques. However, successful experiences of eradication and control in terrestrial and marine ecosystems have been recorded, some of them involving public engagement in management actions. Recognizing the issue as a cross-cutting public policy and developing ongoing governance experiences are fundamental goals for the management of invasive alien species in Brazil.

Resumo Invasões biológicas são uma das maiores ameaças à biodiversidade e à boa qualidade de vida, ocorrendo a partir da translocação de espécies por ação humana. Existem mais de 500 espécies exóticas invadindo ecossistemas atualmente no Brasil, com destaque para plantas e peixes. Pouco se sabe sobre microrganismos invasores. Apesar de existirem espécies exóticas invasoras em todos os ecossistemas no país, a maior parte dos registros foi feita em hábitats com maior interferência humana, como áreas urbanas, periurbanas, terras cultivadas, represas, reservatórios, portos e canais. Historicamente, as regiões sul e sudeste do Brasil apresentam mais espécies exóticas invasoras, mas nas últimas décadas se tem observado um aumento no número de espécies exóticas invasoras nas regiões centro-oeste e norte. O comércio de plantas e peixes ornamentais, assim como o comércio ilegal de mamíferos e répteis silvestres como animais de estimação são algumas das principais vias de introdução e disseminação de espécies exóticas invasoras no Brasil. Sistemas de criação e cultivo que possibilitam o escape para áreas naturais são uma relevante via de introdução em ecossistemas de águas continentais, enquanto introduções não intencionais a partir de navegação e de infraestrutura são de extrema preocupação em ecossistemas marinhos. Os impactos negativos de espécies exóticas invasoras sobre a biota incluem principalmente alterações na estrutura de comunidades e diminuição local da riqueza de espécies nativas, mediados por predação, competição e modificações ecossistêmicas. A maior parte dos impactos negativos registrados ocorreram para espécies introduzidas intencionalmente, como peixes e plantas, mas introduções não intencionais têm levado a impactos na boa qualidade de vida, com custos associados e impactos sobre a saúde humana. A gestão de invasões biológicas esbarra em desafios a serem superados, tais como a falta de conhecimento do público sobre o impacto de espécies exóticas invasoras, o apelo popular de espécies invasoras carismáticas ou utilizadas por humanos e o emprego de técnicas controversas de controle. Entretanto, experiências bem-sucedidas de erradicação e controle em ecossistemas terrestres e marinhos têm sido registrados, alguns deles envolvendo engajamento público nas ações de manejo. Reconhecer o tema como uma política pública transversal e desenvolver experiências continuadas de governança são metas fundamentais para a gestão e o manejo de espécies exóticas invasoras no Brasil.

Proyección didáctica educativa mediante la plataforma Moodle en la disciplina Bases Biológicas de la Medicina

Introducción: Las decisiones tomadas para la conducción adecuada de los procesos académicos comprometen la responsabilidad ante la formación profesional. Objetivo: Analizar la proyección didáctica educativa asumida por el Departamento de Ciencias Básicas Biomédicas, de la Facultad de Ciencias Médicas Salvador Allende, con el uso de la plataforma Moodle en la enseñanza-aprendizaje de la Disciplina Bases Biológicas de la Medicina. Posicionamiento de los autores: Se constató que se ha venido haciendo uso de la plataforma Moodle como un recurso para implementar aulas virtuales complementarias a la docencia presencial, en las que se promueve la participación consciente y activa de los estudiantes en el proceso de su formación, cuyo funcionamiento se propone mejorar. Conclusiones: El análisis sobre las decisiones tomadas por el Departamento de Ciencias Básicas Biomédicas, de la Facultad de Ciencias Médicas Salvador Allende, con respecto al uso docente de la plataforma Moodle, permitió establecer que dichas determinaciones resultan adecuadas, al coincidir con las normas generales que suponen una gestión didáctica pertinente, característica que contribuye al cumplimiento de uno de los indicadores de la responsabilidad social universitaria. Se sugiere promover institucionalmente esta posición acerca del trabajo metodológico en otros departamentos docentes que solamente han desarrollado repositorios de información.

Introduction: Decisions made for conducting academic processes properly compromise the responsibility to respond for professional training. Objective: To analyze the educational-didactic projection assumed by the Department of Basic Biomedical Sciences of Facultad de Ciencias Médicas Salvador Allende with respect to the use of the Moodle platform in the teaching-learning of the discipline Biological Basis of Medicine. Authors' Position: The Moodle platform was found to have been used as a resource to implement virtual classrooms complementary to face-to-face teaching, where promotion is made for the conscious and active participation of students in the process of their training, and whose operation has been suggested to be improved. Conclusions: The analysis on the decisions made by the Department of Basic Biomedical Sciences at Facultad de Ciencias Médicas Salvador Allende with respect to the teaching use of the Moodle platform allowed establishing that such determinations are adequate, as they coincide with the general standards based on a relevant didactic management, a characteristic that contributes to the fulfillment of one of the indicators of university social responsibility. This position towards methodological work is suggested to be promoted institutionally in other teaching departments that have only developed information repositories.

Reflexiones ético-legales entorno a la recogida y análisis de muestras biológicas humanas en los ensayos clínicos promovidos por las compañías farmacéuticas / Reflexions ètico-legals al voltantde la recollida i anàlisi de mostres biològiques humanesenels assaigs clínics promoguts per les companyies farmacèutiques / Ethical and legal considerations on collection and analysis of human biological samples within clinical trials sponsored by the pharmaceutical companies

La recogida de muestras biológicas humanas dentro del contexto de un ensayo clínico promovido por un laboratorio farmacéutico y no relacionada directamente con el ensayo es cada vez más habitual. Este artículo plantea los conflictos bioéticos que dicha recogida, almacenamiento y análisis conllevan, y como afrontarlos.(AU)

La recollida de mostres biològiques humanes dintre del context d ́un assaig clínic promogut per un laboratorio farmacèutic i no relacionada directament amb l ́assaig és cada cop més habitual. Aquest article planteja els conflictes bioètics que aquesta recollida, enmagatzematge i anàlisi comporten i com enfrontar-los.(AU)

The collection of human biological samples within a clinical trial sponsored by a pharmaceutical company and not directly related with the trial it is more and more common. This paper shows the bioethical conflicts regarding the collection, storage, and analysis and how to face them.(AU)

Manual de procesos y procedimientos para análisis clínicos de muestras biológicas / Manual of processes and procedures for clinical analysis of biological simples

El presente manual de procesos y procedimientos documenta las principales actividades de atención integral que se ofrecen al usuario interno y externo para el análisis clínico de muestras biológicas, como parte del proceso de atención en salud integral e integrada a la persona en el curso de vida, describe el sistema de operación de los servicios ofrecidos para el análisis clínico de estas muestras en los establecimientos de salud, mediante el enfoque por procesos, fomentando el desarrollo organizacional y el mejoramiento continuo para el cumplimiento de la misión institucional

This manual of processes and procedures documents the main comprehensive care activities offered to the internal and external user for the clinical analysis of biological samples, as part of the process of comprehensive and integrated health care for the person in the course of life, describes the system of operation of the services offered for the clinical analysis of these samples in health establishments, through the process approach, fostering organizational development and continuous improvement for the fulfilment of the institutional misión

Physiological and Biological Testing and Assessment of Post-Traumatic Stress Disorder / Physiological and Biological Testing and Assessment of Post-Traumatic Stress Disorder / Testes fisiológicos e biológicos e avaliação do Transtorno de Estresse Pós-Traumático

This review essay investigates the biological and physi- ological consequences of PTSD to deepen its academic understanding, alongside an analysis of psychobiologi- cal testing and assessment procedures. Psychological responses to traumatic events can be acute stress reactions or stress disorders. One among them is post-traumatic stress disorder (PTSD). When people experience a trau- matic event, such as death, terror, or physical injury, they tend to demonstrate fear, helplessness, or hopelessness. Patients displaying other symptoms like re-experiencing the trauma, avoidance, or hyper-arousal also indicate PTSD. Experiencing extended PTSD may cause significant health problems, whether biological, such as the dysfunction of stress-responsive neurobiological sys- tems, or physiological, such as hypertension and heart disease. Previous studies of trauma survivors reported a strong link between physical and mental health. The cumulative literature in psychology shows that traumatic exposure can cause disturbing effects in the short and long term. This review will contribute to developing an understanding of the biological markers of PTSD. This paper specifically deals with biological and physiological testing and assessment of PTSD. It includes widely utilized biological assessments and summarizes a general multi-model assessment to identify PTSD symptoms.

Las respuestas psicológicas a acontecimientos traumáticos pueden dar lugar a estrés agudo, trastornos de estrés o trastornos de estrés postraumático (TEPT). Cuando las personas experimentan un evento traumático, como la muerte de un ser querido, terror o daño físico, tienden a mostrar miedo, impotencia o desesperanza. Mostrar otros síntomas como volver a vivir aquellas experiencias, evasión o hiperexcitación indica TEPT. Sufrir el TEPT a largo plazo puede causar problemas de salud importantes, ya sean biológicos, como la disfunción de los sistemas neurobiológicos sensibles al estrés; o fisiológicos, como la hipertensión y enfermedades cardíacas. Sin embargo, la literatura psicológica deja poco o ningún espacio para tales consecuencias sobre la salud. Para proporcionar información sobre este tema, la presente revisión tiene como objetivo investigar las consecuencias biológicas y fisiológicas del TEPT, y las pruebas y evaluaciones psicobiológicas relacionadas. Esta revisión de la literatura puede contribuir al desarrollo de marcadores biológicos de TEPT.

As respostas psicológicas a eventos traumáticos po- dem levar a estresse agudo, transtornos de estresse ou transtorno de estresse pós-traumático (TEPT). Quando as pessoas vivenciam um evento traumático, como a morte de um ente querido, terror ou danos físicos, elas tendem a demonstrar medo, desamparo ou desesperança. Mostrar outros sintomas, como reviver essas experiências, evitação ou hiperexcitação, indica TEPT. Sofrer de TEPT de longa duração pode causar problemas de saúde significativos, sejam eles biológicos, como disfunção de sistemas neurobiológicos sensíveis ao estresse; ou fisiológicos, como hipertensão e doenças cardíacas. No entanto, a literatura psicológica deixa pouco ou nenhum espaço para a discussão de tais consequências para a saúde. Para fornecer informações sobre esse tópico, a presente revisão tem como objetivo investigar as consequências biológicas e fisiológicas do TEPT, assim como testes e avaliações psicobiológicas relacionados. Esta revisão de literatura pode contribuir para

What drives the decision to optimise biological treatment in children and youngsters with juvenile idiopathic arthritis? A discrete-choice experiment.

OBJECTIVE: To analyse factors involved in the decision to optimise biologics in juvenile idiopathic arthritis. METHODS: A "discrete-choice" methodology was used. In a nominal group meeting, factors which may influence physicians' decisions to optimise biological dose were identified, together with decision nodes. 1000Minds® was used to create multiple fictitious clinical scenarios based on the factors identified, and to deploy surveys that were sent to a panel of experts. These experts decided for each item which of two clinical scenarios prompted them to optimise the dose of biologic. A conjoint analysis was carried out, and the partial-value functions and the weights of relative importance calculated. RESULTS: In the nominal group, three decision nodes were identified: (1) time to decide; (2) to maintain/reduce or prolong interval; (3) what drug to reduce. The factors elicited were different for each node and included patient and drug attributes. The presence of macrophage activation syndrome (MAS), systemic involvement, or subclinical inflammation made the decision easier (highest weights). The presence of joints of difficult control and year of debut influenced the decision in some but not all, and in different directions. Immunogenicity, adherence, and concomitant treatments were also aspects taken into account. CONCLUSIONS: The decision to optimise the dose of biological therapy in children and youngster can be divided into several nodes, and the factors, both patient and therapy-related, leading to the decision can be detailed. These decisions taken by experts may be transported to practice, study designs, and guidelines.

What drives the decision to optimise biological treatment in children and youngsters with juvenile idiopathic arthritis? A discrete-choice experiment / Modelo de elección discreta para entender la decisión de optimizar los biológicos en la artritis idiopática juvenil

Objective: To analyse factors involved in the decision to optimise biologics in juvenile idiopathic arthritis. Methods: A “discrete-choice” methodology was used. In a nominal group meeting, factors which may influence physicians’ decisions to optimise biological dose were identified, together with decision nodes. 1000Minds® was used to create multiple fictitious clinical scenarios based on the factors identified, and to deploy surveys that were sent to a panel of experts. These experts decided for each item which of two clinical scenarios prompted them to optimise the dose of biologic. A conjoint analysis was carried out, and the partial-value functions and the weights of relative importance calculated. Results: In the nominal group, three decision nodes were identified: (1) time to decide; (2) to maintain/reduce or prolong interval; (3) what drug to reduce. The factors elicited were different for each node and included patient and drug attributes. The presence of macrophage activation syndrome (MAS), systemic involvement, or subclinical inflammation made the decision easier (highest weights). The presence of joints of difficult control and year of debut influenced the decision in some but not all, and in different directions. Immunogenicity, adherence, and concomitant treatments were also aspects taken into account. Conclusions: The decision to optimise the dose of biological therapy in children and youngster can be divided into several nodes, and the factors, both patient and therapy-related, leading to the decision can be detailed. These decisions taken by experts may be transported to practice, study designs, and guidelines.(AU)

Objetivo: Analizar los factores que intervienen en la decisión de optimizar el biológico en la artritis idiopática juvenil. Métodos: Se utilizó la metodología de «elección discreta». Mediante grupo nominal se identificaron factores potencialmente influyentes en la decisión de optimizar la dosis de biológico y los nodos de decisión. Con 1000Minds® se crearon escenarios clínicos ficticios basados en los factores identificados que se mostraron en encuestas a un panel de expertos. Cada ítem de las encuestas mostraba 2 escenarios clínicos y los expertos elegían el que les llevaría a optimizar el biológico. Se realizó un análisis conjunto, calculándose las funciones de valor parcial y los pesos de importancia relativa. Resultados: Se identificaron 3 nodos de decisión: 1) dilatar decisión o no; 2) mantener/reducir o prolongar el intervalo; y 3) qué fármaco reducir. Los factores identificados varían por nodo e incluyen atributos del paciente y del fármaco. La presencia del síndrome de activación macrofágica, la afectación sistémica o la inflamación subclínica facilitaron la decisión (pesos más altos). La presencia de articulaciones de difícil control y el año de inicio influyeron en la decisión en algunos casos, pero no en todos, y en diferentes direcciones. La inmunogenicidad, la adherencia y los tratamientos concomitantes también fueron aspectos decisivos. Conclusiones: La decisión de optimizar la dosis de biológico en artritis idiopática juvenil se divide en varios nodos y se pueden detallar factores, tanto del paciente como del tratamiento, que determinan la decisión. Estas decisiones de experto pueden transportarse a la práctica, la investigación y las recomendaciones.(AU)

Reliability of social media data in monitoring the global pet trade in ants.

The global pet trade is a major risk to biodiversity and humans and has become increasingly globalized, diversified, digitalized, and extremely difficult to control. With billions of internet users posting online daily, social media could be a powerful surveillance tool. But it is unknown how reliably social media can track the global pet trade. We tested whether Instagram data predicted the geographic distribution of pet stores and the taxonomic composition of traded species in the emerging pet trade in ants (Hymenoptera, Formicidae). We visited 138 online stores selling ants as pets worldwide and recorded the species traded. We scraped ∼38,000 Instagram posts from ∼6300 users referencing ants as pets and analyzed comments on post and geolocation (available for ∼1800 users). We tested whether the number of Instagram users predicted the number of ant sellers per country and whether the species referenced as pets on Instagram matched the species offered in online stores, with a particular focus on invasive species. The location of Instagram users referencing ants as pets predicted the location of ant sellers across the globe (R2  = 0.87). Instagram data detected 439 of the 631 ant species traded in online stores (70%), including 59 of the 68 invasive species traded (87%). The number of Instagram users referencing a species was a good predictor of the number of sellers offering the species (R2  = 0.77). Overall, Instagram data provided affordable and reliable data for monitoring the emerging pet trade in ants. Easier access to these data would facilitate monitoring of the global pet trade and help implement relevant regulations in a timely manner.

El mercado global de mascotas es una amenaza importante para la biodiversidad y los humanos y cada vez está más globalizado, diversificado, digitalizado y muy difícil de controlar. Con miles de millones de usuarios publicando a diario en línea, las redes sociales podrían ser una herramienta poderosa de vigilancia, aunque no se sabe cuán confiable puede ser su rastreo del mercado global de mascotas. Analizamos si los datos de Instagram pronosticaban la distribución geográfica de las tiendas de mascotas y la composición taxonómica de las especies comercializadas en el mercado emergente de hormigas mascotas (Hymenoptera, Formicidae). Visitamos 138 tiendas virtuales dedicadas al comercio de hormigas como mascotas a nivel mundial y registramos las especies comercializadas. Reunimos ∼38,000 publicaciones de Instagram de ∼6,300 usuarios que mencionaban a las hormigas como mascotas y analizamos los comentarios en las publicaciones y la geolocalización (disponible para ∼1,800 usuarios). Analizamos si el número de usuarios de Instagram pronosticaba el número de vendedores de hormigas por país y si las especies mencionadas como mascotas en Instagram eran las mismas que aquellas ofrecidas en las tiendas en línea, con foco particular sobre las especies invasoras. La ubicación de los usuarios de Instagram que mencionaban a las hormigas como mascotas pronosticó la ubicación de los vendedores de hormigas alrededor del mundo (R2 = 0.87). La información de Instagram detectó 439 de las 631 especies de hormigas comercializadas en las tiendas virtuales (70%), incluidas 59 de las 68 especies invasoras comercializadas (87%). El número de usuarios de Instagram que mencionaba a una especie fue un buen indicador del número de vendedores que ofrecían esa eespecie (R2 = 0.77). En general, la información de Instagram proporcionó datos accesibles y confiables para el monitoreo del mercado emergente de hormigas mascotas. Un acceso más sencillo a estos datos facilitaría el monitoreo del mercado global de mascotas y ayudaría a implementar regulaciones relevantes de manera oportuna.

Diversity of fungi obtained from bats captured in urban forest fragments in Sinop, Mato Grosso, Brazil / Diversidade de fungos em morcegos de fragmentos florestais urbanos de Sinop, Mato Grosso, Brasil

Abstract Bats are important for the homeostasis of ecosystems and serve as hosts of various microorganisms including bacteria, viruses, and fungi with pathogenic potential. This study aimed to isolate fungi from biological samples obtained from bats captured in the city of Sinop (state of Mato Grosso, Brazil), where large areas of deforestation exist due to urbanization and agriculture. On the basis of the flow of people and domestic animals, 48 bats were captured in eleven urban forest fragments. The samples were processed and submitted to microbiological cultures, to isolate and to identify the fungal genera. Thirty-four (70.83%) of the captured bats were positive for fungi; 18 (37.5%) and 16 (33.33%) of these bats were female and male, respectively. Penicillium sp., Scopulariopsis sp., Fusarium sp., Aspergillus sp., Alternaria sp., Cryptococcus sp., Trichosporon sp., and Candida sp., which may cause opportunistic infections, were isolated. The bat species with the highest number of fungal isolates was Molossus molossus: 21 isolates (43.8%). According to our results, bats captured in urban forest fragments in Sinop harbor pathogenic fungi, increasing the risk of opportunistic fungal infections in humans and domestic animals.

Resumo Os morcegos apresentam grande importância na homeostasia dos ecossistemas e são hospedeiros de uma rica diversidade de micro-organismos como bactérias, vírus e fungos com potencial patogênico. Portanto, este estudo visou isolar fungos presentes em amostras biológicas de morcegos na cidade de Sinop - MT, que possui grandes áreas de desmatamento devido à urbanização e agricultura. Foram capturados 48 morcegos de diferentes espécies, em onze fragmentos florestais urbanos definidos de acordo com fluxo de pessoas e animais domésticos, para obtenção de amostras biológicas. Essas amostras foram processadas e submetidas aos cultivos microbiológicos, para isolamento e identificação dos gêneros dos fungos. Dos 48 morcegos, 34 (70,83%) foram positivos para pelos menos um gênero de fungo, sendo 18 (37,5%) fêmeas e 16 (33,33%) machos, e os gêneros isolados a partir das amostras biológicas foram Penicillium sp., Scopulariopsis sp., Fusarium sp., Aspergillus sp., Alternaria sp., Cryptococcus sp., Trichosporon sp. e Candida sp., que podem ser causadores de infecções oportunistas. Desse total, a espécie que apresentou maior positividade para pelo menos um gênero de fungo foi Molossus molossus com 21 (43,8%). Nossos resultados demonstram que os morcegos capturados nos fragmentos florestais urbanos na cidade de Sinop - MT, podem atuar como agentes veiculadores de fungos com potencial patogênico, aumentando assim o risco de exposição e aquisição de infecções fúngicas oportunistas por pessoas e animais domésticos.

Diversity of fungi obtained from bats captured in urban forest fragments in Sinop, Mato Grosso, Brazil / Diversidade de fungos em morcegos de fragmentos florestais urbanos de Sinop, Mato Grosso, Brasil

Bats are important for the homeostasis of ecosystems and serve as hosts of various microorganisms including bacteria, viruses, and fungi with pathogenic potential. This study aimed to isolate fungi from biological samples obtained from bats captured in the city of Sinop (state of Mato Grosso, Brazil), where large areas of deforestation exist due to urbanization and agriculture. On the basis of the flow of people and domestic animals, 48 bats were captured in eleven urban forest fragments. The samples were processed and submitted to microbiological cultures, to isolate and to identify the fungal genera. Thirty-four (70.83%) of the captured bats were positive for fungi; 18 (37.5%) and 16 (33.33%) of these bats were female and male, respectively. Penicillium sp., Scopulariopsis sp., Fusarium sp., Aspergillus sp., Alternaria sp., Cryptococcus sp., Trichosporon sp., and Candida sp., which may cause opportunistic infections, were isolated. The bat species with the highest number of fungal isolates was Molossus molossus: 21 isolates (43.8%). According to our results, bats captured in urban forest fragments in Sinop harbor pathogenic fungi, increasing the risk of opportunistic fungal infections in humans and domestic animals.

Os morcegos apresentam grande importância na homeostasia dos ecossistemas e são hospedeiros de uma rica diversidade de micro-organismos como bactérias, vírus e fungos com potencial patogênico. Portanto, este estudo visou isolar fungos presentes em amostras biológicas de morcegos na cidade de Sinop - MT, que possui grandes áreas de desmatamento devido à urbanização e agricultura. Foram capturados 48 morcegos de diferentes espécies, em onze fragmentos florestais urbanos definidos de acordo com fluxo de pessoas e animais domésticos, para obtenção de amostras biológicas. Essas amostras foram processadas e submetidas aos cultivos microbiológicos, para isolamento e identificação dos gêneros dos fungos. Dos 48 morcegos, 34 (70,83%) foram positivos para pelos menos um gênero de fungo, sendo 18 (37,5%) fêmeas e 16 (33,33%) machos, e os gêneros isolados a partir das amostras biológicas foram Penicillium sp., Scopulariopsis sp., Fusarium sp., Aspergillus sp., Alternaria sp., Cryptococcus sp., Trichosporon sp. e Candida sp., que podem ser causadores de infecções oportunistas. Desse total, a espécie que apresentou maior positividade para pelo menos um gênero de fungo foi Molossus molossus com 21 (43,8%). Nossos resultados demonstram que os morcegos capturados nos fragmentos florestais urbanos na cidade de Sinop - MT, podem atuar como agentes veiculadores de fungos com potencial patogênico, aumentando assim o risco de exposição e aquisição de infecções fúngicas oportunistas por pessoas e animais domésticos.

Diversity of fungi obtained from bats captured in urban forest fragments in Sinop, Mato Grosso, Brazil

Abstract Bats are important for the homeostasis of ecosystems and serve as hosts of various microorganisms including bacteria, viruses, and fungi with pathogenic potential. This study aimed to isolate fungi from biological samples obtained from bats captured in the city of Sinop (state of Mato Grosso, Brazil), where large areas of deforestation exist due to urbanization and agriculture. On the basis of the flow of people and domestic animals, 48 bats were captured in eleven urban forest fragments. The samples were processed and submitted to microbiological cultures, to isolate and to identify the fungal genera. Thirty-four (70.83%) of the captured bats were positive for fungi; 18 (37.5%) and 16 (33.33%) of these bats were female and male, respectively. Penicillium sp., Scopulariopsis sp., Fusarium sp., Aspergillus sp., Alternaria sp., Cryptococcus sp., Trichosporon sp., and Candida sp., which may cause opportunistic infections, were isolated. The bat species with the highest number of fungal isolates was Molossus molossus: 21 isolates (43.8%). According to our results, bats captured in urban forest fragments in Sinop harbor pathogenic fungi, increasing the risk of opportunistic fungal infections in humans and domestic animals.

Resumo Os morcegos apresentam grande importância na homeostasia dos ecossistemas e são hospedeiros de uma rica diversidade de micro-organismos como bactérias, vírus e fungos com potencial patogênico. Portanto, este estudo visou isolar fungos presentes em amostras biológicas de morcegos na cidade de Sinop - MT, que possui grandes áreas de desmatamento devido à urbanização e agricultura. Foram capturados 48 morcegos de diferentes espécies, em onze fragmentos florestais urbanos definidos de acordo com fluxo de pessoas e animais domésticos, para obtenção de amostras biológicas. Essas amostras foram processadas e submetidas aos cultivos microbiológicos, para isolamento e identificação dos gêneros dos fungos. Dos 48 morcegos, 34 (70,83%) foram positivos para pelos menos um gênero de fungo, sendo 18 (37,5%) fêmeas e 16 (33,33%) machos, e os gêneros isolados a partir das amostras biológicas foram Penicillium sp., Scopulariopsis sp., Fusarium sp., Aspergillus sp., Alternaria sp., Cryptococcus sp., Trichosporon sp. e Candida sp., que podem ser causadores de infecções oportunistas. Desse total, a espécie que apresentou maior positividade para pelo menos um gênero de fungo foi Molossus molossus com 21 (43,8%). Nossos resultados demonstram que os morcegos capturados nos fragmentos florestais urbanos na cidade de Sinop - MT, podem atuar como agentes veiculadores de fungos com potencial patogênico, aumentando assim o risco de exposição e aquisição de infecções fúngicas oportunistas por pessoas e animais domésticos.

Migraine treatment with biological therapies. The state of the art / Tratamento da enxaqueca com terapias biológicas. O Estado da arte

Migraine is a highly prevalent and debilitating neurological disorder. Most patients do not receive a correct diagnosis and effective treatments. Apart of the few specialists and tertiary centers worldwide, the treatment of migraine is usually symptomatic and prevention, as well as treatments of the underlying mechanisms, are not aimed. It results in frustration and substantial burden. The last few years witnessed the releasing of specific biological therapies, mostly addressing one of the peptides involved in migraine pathophysiology, the calcitonin gene-related peptide (CGRP). Either the small molecules as well as the monoclonal antibodies against CGRP or its canonical receptor have been launched in markets across the globe and represent interesting options for the treatment of migraine. Onabotulinumtoxin A has also been proposed for chronic migraine as well, but not for episodic migraine, based on its unique ability to inhibit the SNARE complex formation and the release of numerous potential mediators of migraine. However, despite the favorable figures on efficacy and tolerability of these compounds, the regulations, and particulars of different countries, regarding the structures and reimbursement of medical care, demonstrated different adhesion profiles of chosen populations to receive these emerging weapons against migraine-imposed suffering. This review addresses the use and characteristics of biological therapies used in migraine treatment.

A enxaqueca é um distúrbio neurológico altamente prevalente e debilitante. A maioria dos pacientes não recebe um diagnóstico correto e tratamentos eficazes. Com exceção dos poucos especialistas e centros terciários em todo o mundo, o tratamento da enxaqueca é geralmente sintomático e a prevenção, bem como o tratamento dos mecanismos subjacentes, não são direcionados. Isso resulta em frustração e fardo substancial. Os últimos anos testemunharam o lançamento de terapias biológicas específicas, abordando principalmente um dos peptídeos envolvidos na fisiopatologia da enxaqueca, o peptídeo relacionado ao gene da calcitonina (CGRP). Tanto as pequenas moléculas como os anticorpos monoclonais contra CGRP ou o seu receptor canônico foram lançados em mercados em todo o mundo e representam opções interessantes para o tratamento da enxaqueca. A onabotulinumtoxina A também foi proposta para enxaqueca crônica, mas não para enxaqueca episódica, com base em sua capacidade única de inibir a formação do complexo SNARE e a liberação de numerosos mediadores potenciais da enxaqueca. No entanto, apesar dos números favoráveis ​​sobre a eficácia e tolerabilidade destes compostos, os regulamentos e particularidades de diferentes países, no que diz respeito às estruturas e reembolso dos cuidados médicos, demonstraram diferentes perfis de adesão das populações escolhidas para receber estas armas emergentes contra o sofrimento imposto pela enxaqueca. Esta revisão aborda o uso e as características das terapias biológicas utilizadas no tratamento da enxaqueca.

Análisis bibliométrico de la producción científica y contribuciones en el campo biomédico de una facultad peruana de ciencias biológicas

La producción científica de las facultades de Ciencias Biológicas en el campo biomédico no ha sido evaluada en el Perú. El objetivo del estudio fue analizar las características de la producción científica y las contribuciones a las ciencias biomédicas de la Facultad de Ciencias Biológicas de la Universidad Nacional Pedro Ruiz Gallo, Perú (FCCBB-UNPRG). Se realizó un estudio descriptivo con enfoque bibliométrico; se consultaron tres bases de datos (Scopus, PubMed y LILACS) para buscar artículos que tuvieran, mínimo, un autor afiliado a la FCCBB-UNPRG. Se consignaron los siguientes datos: tipo de publicación, idioma, datos de autoría, financiamiento, año, área de publicación, datos de la revistas e instituciones colaborativas. Se realizó un análisis descriptivo y se elaboraron mapas de redes, utilizando el software VOSviewer. Se recopilaron 46 documentos; la mayoría son artículos (n = 36, 78,26 %), cartas (n = 9, 19,57 %) y reporte de casos (n = 1, 2,17 %); publicados en 30 revistas científicas, principalmente extranjeras. Existió mayor contribución en las áreas de microbiología, virología y parasitología. Se involucraron 42 instituciones colaborativas. Cerca del 90 % de las publicaciones no fueron financiadas. En los últimos años se observó un crecimiento en el número de publicaciones en diferentes áreas temáticas; esto demuestra el compromiso de la FCCBB-UNPRG con la investigación en el campo biomédico y la formación de investigadores. Se espera la implementación de políticas de investigación que involucren pautas y/o directrices para lograr un incremento sostenido de la producción científica.

The scientific production of the faculties of Biological Sciences in the biomedical field has not been evaluated in Peru. The objective of this study was to analyze the characteristics of the scientific production and the contributions to the biomedical sciences of the Faculty of Biological Sciences of Pedro Ruiz Gallo National University, Peru (FCCBB-UNPRG). A descriptive study with a bibliometric approach was carried out. Three databases (Scopus, PubMed, and LILACS) were consulted to search for articles that had, at least, one author affiliated with the FCCBB-UNPRG. The following data were recorded: type of publication, language, authorship data, funding, year, publication area, journal data, and collaborating institutions. A descriptive analysis was carried out and network maps were elaborated, using the VOSviewer software. Forty-six documents were retrived; the majority were articles (n = 36, 78.26%), letters (n = 9, 19.57%) and case reports (n = 1, 2.17%); published in 30 scientific journals, mainly foreign. Greater contribution was observed in microbiology, virology and parasitology. Fotry-two collaborative institutions were involved. About 90% of the publications were not funded. A growth in the number of publications in different thematic areas has been noted in recent years, which demonstrates the commitment of FCCBB-UNPRG to research in the biomedical field and the training of researchers. The implementation of research policies including guidelines and/or directives is expected to achieve sustained increase in scientific production.

Estudio correlacional de complicaciones técnicas, biológicas, estéticas y nivel de satisfacción en tratamientos realizados en la Cátedra de Prótesis de Coronas y Puentes II de la Facultad de Odontología de la Universidad Nacional de Asunción

El objetivofue determinar la asociación correlacional que pudiera presentarse entre las complicaciones técnicas, biológicas y estéticas y el nivel de satisfacción de los tratamientos realizados en la Cátedra de Prótesis de Coronas y Puentes II de la Facultad de Odontología de la UNA en el 2019. Este fue un estudio observacional descriptivo, analítico, de corte transverso. Se incluyeron pacientes que recibieron tratamientos del 2016 al 2018. Acudieron 59 pacientes para la evaluación clínica y aplicación del cuestionario. Se analizaron los datos según número de tratamientos encontrados en cada sujeto, por lo que se calcularon los resultados según 119 tratamientos. Las complicaciones biológicas pulpares fueron la sensibilidad o molestia al frio o al calor en los pilares en un 20.1%. Se encontró asociación muy significativa de las complicaciones biológicas pulpares (p>001) con estado civil, complicaciones biológicas periodontales, complicaciones estéticas cervicales. Las complicaciones biológicas periodontales fueron en mayor frecuencia la gingivitis alrededor de la restauración en un 40,4%. El nivel de satisfacción con el tratamiento recibido se presentó favorable en un 89,1% y asociación muy significativa (p>001) con grado de instrucción y significativa (p>005) con estado civil.Se encontró asociación muy significativa de las complicaciones estéticas cervicales, las complicaciones estéticas de forma y significativa de las complicaciones biológicas periodontales con el nivel de satisfacción.

The objective was to determine the correlational association that could occur between technical, biological and esthetic complications and level of satisfaction in the treatments performed in the Department of Crown and Bridge Prosthodontics II of the Faculty of Dentistry of the National University of Asuncion in 2019. This was a descriptive, analytical, cross-sectional, observational study. Patients who received treatments from 2016 to 2018 were included. Fifty-nine patients attended for clinical evaluation and application of the questionnaire. The data were analyzed according to the number of treatments found in each subject, by which the results were calculated according to 119 treatments. Pulpal biological complications were sensitivity or discomfort to cold or heat in 20.1% of the abutments. A highly significant association was found between pulpal biological complications (p>001) and marital status, periodontal biological complications and cervical esthetic complications. The most frequent periodontal biological complications were gingivitis around the restoration (40.4%). The level of satisfaction with the treatment received was favorable in 89.1% and there was a very significant association (p>001) with educational level and significant (p>005) with marital status. A highly significant association was found between cervical esthetic complications, esthetic complications of shape and periodontal biological complications and the level of satisfaction.

Accidente lonómico leve atribuido a la oruga de la especie Lonomia obliqua en el departamento de Santander, Colombia, 2021 / Mild lonomic accident attributed to the caterpillar of the lonomia obliqua species in the department of Santander, Colombia, 2021

Las orugas del género Lonomia, del orden Lepidoptera y familia Saturnidae, son de interés médico en Sudamérica por ser causantes del lonomismo, tipo de envenenamiento que aumenta cada vez más en Colombia, con tasas de mortalidad de 2,5 %. La severidad es variable y los casos no son de notificación obligatoria, lo que lleva a un subregistro de estos eventos. Se presenta el caso de una mujer adulta joven, quien acude a urgencias por la picadura de 20 orugas Lonomia en la palma de la mano izquierda; presentó signos locales inflamatorios, dolor y limitación de la movilidad de la mano. Se hospitalizó por tres días, se clasificó como leve y se trató con analgesia y antihistamínico endovenoso, lo que logró favorable evolución. El envenenamiento por oruga Lonomia es una urgencia que puede ser mortal, por tanto, es importante que se conozcan estos eventos en la literatura para su adecuado abordaje.

Caterpillars of the genus Lonomia, of the order Lepidoptera, family Saturnidae are of medical interest in South America for being the cause of lonomism, poisoning that is increasing more and more in Colombia, with mortality rates of 2.5%, the severity is variable and they are not mandatory notification, which leads to an underreporting of these events. We present the case of a young adult woman, who went to the emergency room due to the bite of 20 Lonomia caterpillars in the palm of her left hand, generating local inflammatory signs, pain and limitation of hand mobility. She was hospitalized for 3 days, classified as mild and treated with analgesia and intravenous antihistamine; which achieved favorable evolution. Therefore, it was concluded that Lonomia caterpillar poisoning is an emergency, which can be fatal. It is important that these events are known in the literature for their proper approach.