Visual Field Restriction in the Recognition of Basic Facial Expressions: A Combined Eye Tracking and Gaze Contingency Study.

Uncertainties and discrepant results in identifying crucial areas for emotional facial expression recognition may stem from the eye tracking data analysis methods used. Many studies employ parameters of analysis that predominantly prioritize the examination of the foveal vision angle, ignoring the potential influences of simultaneous parafoveal and peripheral information. To explore the possible underlying causes of these discrepancies, we investigated the role of the visual field aperture in emotional facial expression recognition with 163 volunteers randomly assigned to three groups: no visual restriction (NVR), parafoveal and foveal vision (PFFV), and foveal vision (FV). Employing eye tracking and gaze contingency, we collected visual inspection and judgment data over 30 frontal face images, equally distributed among five emotions. Raw eye tracking data underwent Eye Movements Metrics and Visualizations (EyeMMV) processing. Accordingly, the visual inspection time, number of fixations, and fixation duration increased with the visual field restriction. Nevertheless, the accuracy showed significant differences among the NVR/FV and PFFV/FV groups, despite there being no difference in NVR/PFFV. The findings underscore the impact of specific visual field areas on facial expression recognition, highlighting the importance of parafoveal vision. The results suggest that eye tracking data analysis methods should incorporate projection angles extending to at least the parafoveal level.

Leptodelphax maculigera (Hemiptera: Delphacidae) Harbors the Corn Stunt Complex Pathogens.

The African planthopper Leptodelphax maculigera (Hemiptera: Delphacidae) has been recently reported in many places in Brazil in association with maize. Its occurrence in maize production fields in Brazil has brought concerns to the corn production chain regarding the possibility of this planthopper to be a vector for maize bushy stunt phytoplasma (MBSP), corn stunt spiroplasma (Spiroplasma kunkelii), maize rayado fino virus (MRFV), and maize striate mosaic virus (MSMV). The phytoplasma and spiroplasma, which are bacteria belonging to the class Mollicutes, and the two viruses are associated with the corn stunt disease complex. Given the presence of the African planthopper species and the corn stunt complex in Brazil, we further investigated the abundance of this planthopper species in the State of Santa Catarina, Brazil, and whether the planthopper can carry the four pathogens. We inspected 12 maize production fields in different municipalities in the state for 20 weeks, using two yellow sticky traps for each maize field. The sticky traps were replaced weekly. A total of 130 specimens of L. maculigera were captured, with a great discrepancy in quantity among locations and weeks. We detected the mollicute MBSP and the viruses MRFV and MSMV in L. maculigera, whereas S. kunkelii was absent in the assessed African planthopper samples. The molecular detection of the phytoplasma and the viruses in field-collected African planthoppers is strong evidence that this insect species has the ability to acquire those pathogens through feeding from the phloem of diseased maize plants. Nonetheless, transmission capacity needs to be experimentally proven to assert L. maculigera as a vector for the corn-stunting pathogens.

Condemnation of bovine carcasses due to tuberculosis-gross lesions in Rio Grande do Sul, Brazil: Associated risk factors.

Surveillance of bovine tuberculosis (bTB) lesions in animals at slaughterhouses is useful for controlling and eradicating the disease, besides providing epidemiological information. This study aimed to identify risk factors for bovine tuberculosis (bTB) condemnation in cattle at slaughterhouses in Rio Grande do Sul, Brazil. A logistic regression analysis was conducted using data on bTB-related condemnations. Variables examined included animal origin, number of slaughtered animals, season, inspection level (state or municipality), animal sex, and slaughterhouse location. A total of 297,817 Animal Transport Guides were evaluated, representing the transportation of 3497,521 animals. Among these, 6097 (2.05%) had at least one animal condemned due to bTB lesions. Risk factors for condemnation included larger batch sizes, female animals, slaughterhouses, and animal origin. The higher condemnation frequency in females and regions with dairy farms suggests links to milk production. Variation in condemnation rates by inspection level and slaughterhouse highlights the need for standardized procedures in identifying bTB lesions. Identifying these risk factors enables targeted interventions to enhance disease control and eradication efforts.

New method of video-assisted vaginoscopy in Nellore heifers.

Vaginoscopy allows an intravaginal endoscopic evaluation and can help in the diagnosis of female bovine genital tract disorders. The aim of this study is to validate a new method of gynaecological examination using a Scope VOR&GDI videovaginoscope. Twenty-six heifers were used, divided into 2 groups with 13 animals, control group (GC) and videovaginoscopic group (GV). In the CG, vaginoscopy was performed with a vaginal speculum and in the GV with a Scope VOR&GDI videovaginoscope. All heifers underwent vaginoscopy on day 0 (D0), and 9 days later, on day 9 (D9). Vaginoscopy provided adequate intravaginal inspection. In the CG, 23% (3/13) of the heifers showed discomfort during the test. In GV, vaginal inspection was better due to better image quality. The videovaginoscope is the most effective equipment for carrying out the vaginoscopy procedure in Nellore heifers, as it produces sharper and clearer images and can help in the diagnostic and therapeutic routine of veterinarians.

Prevalence of cysticercosis in cattle slaughterhouses in the western region of Minas Gerais, Brazil (2013-2020): Influence of sanitary regulations in reducing risks to human health.

Bovine cysticercosis is a foodborne zoonosis that has a worldwide distribution, and its highest prevalence is found in developing countries with a large volume of cattle production, such as Brazil. The objective was to evaluate the prevalence of cysticercosis during seven years in three slaughterhouses located in the western region of Minas Gerais state, Brazil, and the impacts of the legislation on the identification and destination of infected carcasses. Three slaughterhouses (Sl) were selected for this study with averages of 200 (SlA), 721 (SlB) and 1138 (SlC) bovines slaughtered per day. The monthly number of slaughtered cattle, infected carcasses with cysticercosis and destination were used to calculate the condemnation occurrence index (COI) and the adjusted seasonal index (ASI). To assess the impact of the legislation, the prevalence rate and destination of infected carcasses were subdivided into the PRE (January 2013 to March 2017) and POST (April 2017 to December 2020) period of Decree 9013 of 2017 and the results were compared by chi-square (P < 0.05). SlA recorded the highest cysticercosis rate with 1.54 % and SlC the lowest rate with 0.33 %. In addition, it was observed that in the POST period there was an increase in the identification of infected carcasses in SlA and B (P < 0.05). The POST period also resulted in a change in the relative frequency of infected carcasses destination in the three slaughterhouses (P < 0.05). The ASI identified an increase in infected carcasses in February and a decrease in November for SlB, proving to be an important tool for planning the inspection team trainings. These results show that the disease is still present in the studied region and highlight the benefits of using the One Health concept in the evaluation of a time series prevalence of bovine cysticercosis by: identifying seasonality of the disease, trends during the time period and impacts brought by updates in the national sanitary legislation.

Squamocolumnar junction visibility among cervical cancer screening population in Peru might influence upper age for screening programs.

Cervical cancer screening and treatment of screen positives is an important and effective strategy to reduce cervical cancer morbidity and mortality. In order to have an accurate cervical cancer screening and evaluation of positives, the entire Squamocolumnar Junction (SCJ) must be visible. Throughout the life course, the position of the SCJ changes and affects its visibility. SCJ visibility was analyzed among participants screened at the League Against Cancer Clinic in Lima, Peru. Of the 4247 participants screened, the SCJ was fully visible in 49.7% of participants, partially visible in 23.1%, and not visible in 27.2%. Visibility decreased with age, and by age 45 years old, the SCJ was not fully visible in over 50% of participants. Our results show that a high percentage of participants at ages still recommended for screening do not have totally visible SCJ, and we may need to reconsider the upper age limit for screening and find new strategies for evaluation of those with a positive screening test and non-visible SCJ.

Proposal of a regulatory index of quality of water supply services-RIQS.

Among the challenges faced by regulatory authorities in the water sector, the large number of municipal supply services to be inspected and the cost of on-site inspections are prominent. To overcome these issues, decisions regarding the priority of inspections based on indicators is an alternative. Therefore, this research aims to propose and evaluate the Regulatory Index of Quality of Water Supply Service (RIQS) to triage on-site inspections of water supply systems in cities of the state of Minas Gerais. The study was conducted with information from the Regulatory Agency of Water Supply and Sanitation Services of Minas Gerais (Arsae-MG). The methodology followed seven steps: (i) selection of available indicators; (ii) grouping of indicators according to their typology; (iii) screening of indicators; (iv) establishment of standardized scale; (v) evaluation of the relative importance of typologies and indicators, through the adaptation of the analytic hierarchy process (AHP); (vi) determination of the RIQS; and (vii) analysis of results. As a result, we selected 12 indicators to compose the RIQS, which deal with efficiency, effectiveness, and customer relationship. We noticed that the indicator of water supply service coverage (17.2%) had the highest weight in the calculation of the RIQS, and the index of requests for an inspection of the water connection served on time (2.1%) had the lowest weight. In addition, 95.1% of the 591 municipalities evaluated presented excellent or good performance. Based on these results, we show that the RIQS can be used to identify cities with low performance and prioritize inspections in the most urgent water systems. Furthermore, these outcomes reveal the possibility of extending and adapting the methodology to other regulatory agencies around the world for identifying the priority of inspections in water supply systems at a municipal level.

Drone-Robot to Clean Power Line Insulators.

The inspection and maintenance of transmission systems are necessary for their proper functioning. In this way, among the line's critical points are the insulator chains, which are responsible for providing insulation between conductors and structures. The accumulation of pollutants on the insulator surface can cause failures in the power system, leading to power supply interruptions. Currently, the cleaning of insulator chains is performed manually by operators who climb towers and use cloths, high-pressure washers, or even helicopters. The use of robots and drones is also under study, presenting challenges to be overcome. This paper presents the development of a drone-robot for cleaning insulator chains. The drone-robot was designed to identify insulators by camera and perform cleaning through a robotic module. This module is attached to the drone and carries a battery-powered portable washer, a reservoir for demineralized water, a depth camera, and an electronic control system. This paper includes a literature review on the state of the art related to strategies used for cleaning insulator chains. Based on this review, the justification for the construction of the proposed system is presented. The methodology used in the development of the drone-robot is then described. The system was validated in a controlled environment and in field experimental tests, with the ensuing discussions and conclusions formulated, along with suggestions for future work.

Irregularidades registradas durante inspeções sanitárias em restaurantes e similares / Irregularities recorded during sanitary inspections in restaurants and similar

Identificar irregularidades em restaurantes pode auxiliar a minimizar riscos de contaminação. O objetivo desse estudo foi analisar as irregularidades registradas em inspeções sanitárias de restaurantes e similares em Curitiba, Brasil, entre 2005 a 2017. A descrição das irregularidades foi organizada seguindo as categorias e os itens da legislação de Boas Práticas (BP) para serviços de alimentação do Brasil e as cinco chaves para uma alimentação mais segura, definidas pela Organização Mundial da Saúde. Do total de 4.098 registros, houve a descrição de 8.327 irregularidades. Em relação às BP, houve a prevalência da descrição de irregularidades nos anos de 2005, 2006 e 2013, e das categorias: edificações, instalações, equipamentos, móveis e utensílios (42,80%); preparação do alimento (11,54%); documentação e registro (9,92%) e matéria-prima, ingredientes e embalagens (9,06%). Em relação às cinco chaves para uma alimentação mais segura, apenas 3.209 (38,53%) descrições correspondiam a esse tema, com destaque a: manter a limpeza (40,70%) e usar água e matérias-primas seguras (34,71%). Houve uma tendência decrescente na descrição das irregularidades no período estudado, porém ainda é um desafio implementar as BP em restaurantes e similares no Brasil, o que pode favorecer a ocorrência de doenças e aumentar o risco à saúde da população.

Identifying irregularities in restaurants can help minimize contamination risks. The objective of this study was to analyze the irregularities recorded in sanitary inspections of restaurants and similar establishments in Curitiba, Brazil, between 2005 and 2017. The description of the irregularities was categorized following the items and criteria outlined in the Good Practices (GP) legislation for food services of Brazil and the Five Keys to Safer Food, defined by the World Health Organization. Out of the total of 4,098 records, 8,327 irregularities were documented. Concerning GP, there was a prevalence of irregularities described in the years 2005, 2006 and 2013, mainly in the following categories: buildings, installations, equipment, furniture and utensils (42.80%); food preparation (11.54%); documentation and registration (9.92%) and raw materials, ingredients and packaging (9.06%). Regarding the Five Keys to Safer Food, only 3,209 (38.53%) corresponded to this theme, with particular emphasis on maintaining cleanliness (40.70%) and using safe water and raw materials (34.71 %). The study observed a declining trend in the description of irregularities over the study period. However, the successful implementation of GP in restaurants and similar establishments in Brazil remains a challenge, potentially favoring the occurrence of foodborne diseases and increasing the risk to public health.

Estudo metanalítico sobre a microbiologia da carne bovina moída no Brasil / Meta-analytical study on the microbiology of ground beef in Brazil

RESUMO Objetivou-se avaliar a qualidade microbiológica da carne bovina moída por meio de um estudo metanalítico. Para o estudo metanalítico, utilizou-se como base de dados o Google Scholar Brasil, concentrando-se no período de busca entre outubro de 2020 e junho de 2021. Foram avaliados 108 estudos científicos com as variáveis analisadas referentes a contagens de bactérias mesófilas, bactérias psicrotróficas, coliformes totais, coliformes termotolerantes, Staphylococcus aureus e Salmonella sp. em unidade formadora de colônia por grama de carne (UFC.g-1). Os dados foram submetidos a uma análise descritiva e distribuição normal por histograma de frequência. As contagens de bactérias mesófilas apresentaram uma média de 3,09 UFC.g-1 com coeficiente de variação de 68,53%. As bactérias psicrotróficas apresentaram 27,27% dos dados levantados próximos a 5,60 UFC.g-1. Com relação aos coliformes termotolerantes, 40% dos dados levantados estavam com contagens entre 0,90 e 40% em 3,10 UFC.g-1. Oito estudos apresentaram efeitos (P < 0,001) sobre a presença se Salmonella sp. na carne moída, sendo este estudo metanalítico classificado como heterogêneo (Chi2 = 80,39; P < 0,001) e com inconsistência alta (I2 = 81%). A metanálise constatou baixa contagem de bactérias mesófilas e Staphylococcus aureus em pesquisas brasileiras que abordassem a qualidade microbiológica da carne moída bovina. Estudos metanalíticos como o proposto são necessários para identificar os problemas relacionados à transmissão de doenças em alimentos. Observou-se que, nos achados brasileiros, Staphylococcus aureus e mesófilos foram encontrados em menor quantidade, com maior presença de Samonella sp.

ABSTRACT This paper evaluates the microbiological quality of ground beef through a meta-analytic study using Google Scholar Brazil database, with a focus on the search period between October 2020 and June 2021. A total of 108 scientific studies were evaluated with the analyzed variables, referring to the counts of mesophilic bacteria, psychrotrophic bacteria, total coliforms, thermotolerant coliforms, Staphylococcus aureus and Salmonella sp. In colony forming unit per gram of meat (CFU.g-1). Data were subjected to descriptive analysis and normal distribution by frequency histogram. The counts of mesophilic bacteria presented an average of 3.09 CFU.g-1, with a coefficient of variation of68.53%. Psychrotrophic bacteria represented 27.27% of the data collected, close to 5.60 CFU.g-1. Regarding thermotolerant coliforms, 40% of the data collected had counts between 0.90 and 40% at 3.10 CFU.g-1. Eight studies showed effects (p < 0.001) on the presence of Salmonella sp. in ground beef. This meta-analytic study was classified as heterogeneous (Chi2 = 80.39; p < 0.001) and with high inconsistency (P = 81%). Besides, the meta-analysis allowed identifying low counts of mesophilic bacteria and Staphylococcus aureus in Brazilian studies addressing the microbiological quality of ground beef.

Image-Based Detection of Modifications in Assembled PCBs with Deep Convolutional Autoencoders.

In this paper, we introduce a one-class learning approach for detecting modifications in assembled printed circuit boards (PCBs) based on photographs taken without tight control over perspective and illumination conditions. Anomaly detection and segmentation are essential for several applications, where collecting anomalous samples for supervised training is infeasible. Given the uncontrolled environment and the huge number of possible modifications, we address the problem as a case of anomaly detection, proposing an approach that is directed towards the characteristics of that scenario, while being well suited for other similar applications. We propose a loss function that can be used to train a deep convolutional autoencoder based only on images of the unmodified board-which allows overcoming the challenge of producing a representative set of samples containing anomalies for supervised learning. We also propose a function that explores higher-level features for comparing the input image and the reconstruction produced by the autoencoder, allowing the segmentation of structures and components that differ between them. Experiments performed on a dataset built to represent real-world situations (which we made publicly available) show that our approach outperforms other state-of-the-art approaches for anomaly segmentation in the considered scenario, while producing comparable results on a more general object anomaly detection task.

Análise microbiológica de sushi comercializado em Rio Branco, Acre / Microbiological analysis of sushi marketed in Rio Branco, Acre / Análisis microbiológico de sushi comercializado en Rio Branco, Acre

O Brasil é um dos países mais diversificados no ramo gastronômico oferecendo vários alimentos diferentes aos seus consumidores, com base nos próprios pratos típicos ou provenientes de outras culturas. O pescado trata-se de um alimento perecível que necessita de atenções especiais em seu processamento. Falhas nas condições higiênico-sanitárias, associadas com a não cocção do alimento, podem ocasionar em uma contaminação e proliferação de bactérias, o que leva à uma grande preocupação a nível de saúde pública. O estudo analisou os aspectos microbiológicos de sushi comercializado na cidade de Rio Branco ­ Acre verificando os parâmetros de qualidade e as condições higiênicas sanitárias, comparando os resultados obtidos com a legislação vigente estabelecida pela ANVISA. Foram escolhidos 5 estabelecimentos aleatoriamente, sendo escolhidas 3 amostras de sushis do tipo niguiri de cada. As análises microbiológicas incluíram coliformes totais e coliformes termotolerantes utilizando a técnica dos tubos multiplos e a técnica de semeadura por profundidade para mesófilos e Salmonella. Constatou-se que todas as amostras tiveram um crescimento bacteriano e presença sugestiva de Salmonella, tornando o alimento impróprio para o consumo e mostrando uma falha nas condições higiênico- sanitária ao qual o sushi é processado e armazenado. É necessário maior fiscalização dos órgãos responsáveis e cuidado dos estabelecimentos que vendem sushi na cidade de Rio Branco, para que o produto vendido seja de boa qualidade e não cause malefícios a saúde de quem o consome.(AU)

Brazil is one of the most diversified countries in the gastronomic field, offering several different foods to its consumers, based on typical dishes or from other cultures. Fish is a perishable food that requires special attention in its processing. Failures in hygienic-sanitary conditions, coupled with the consumption of undercooked food, can lead to contamination and the proliferation of bacteria, which raises significant concerns regarding public health. The study analyzed the microbiological aspects of sushi sold in the city of Rio Branco - Acre, verifying the quality parameters and the hygienic sanitary conditions, comparing the obtained results with the current legislation established by ANVISA. Five establishments were randomly selected, and three samples of nigiri sushi were chosen from each establishment. The microbiological analysis included total coliforms and thermotolerant coliforms using the multiple tube technique, as well as depth seeding technique for mesophiles and Salmonella. It was found that all samples exhibited bacterial growth and suggested the presence of Salmonella, rendering the food unsuitable for consumption and indicating a failure in the hygienic-sanitary conditions under which the sushi was processed and stored. Greater inspection by the responsible authorities and improved care by establishments selling sushi in the city of Rio Branco are necessary to ensure that the product sold is of good quality and does not pose harm to the health of consumers.(AU)

Brasil es uno de los países más diversificados en el campo gastronómico, ofreciendo muchos alimentos diferentes a sus consumidores, basados en platos típicos ode otras culturas El pescado es un alimento perecedero que necesita especial atención en su elaboración. Las fallas en las condiciones, higiénico-sanitarias asociadas a la no cocción de los alimentos, pueden conducir a la contaminación y proliferación de bacterias, lo que genera una gran preocupación en términos de salud pública. El estudio analizó los aspectos microbiológicos del sushi comercializado en la ciudad de Rio Branco - Acre, verificando los parámetros de calidad y las condiciones higiénicas sanitarias, comparando los resultados obtenidos con la legislación vigente establecida por la ANVISA. Se eligieron 5 establecimientos al azar, y de cada uno se escogieron 3 muestras de sushi niguiri. Los análisis microbiológicos incluyeron coliformes totales y coliformes termotolerantes mediante la técnica de tubos múltiples y la técnica de siembra profunda para mesófilos y Salmonella. Se encontró que todas las muestras presentaban crecimiento bacteriano y la sugestiva presencia de Salmonella, lo que hace que el alimento no sea apto para el consumo y presenta una falla en las condiciones higiénico-sanitarias en las que se procesa y almacena el sushi. Se necesita mayor fiscalización por parte de los órganos responsables y cuidado de los establecimientos que venden sushi en la ciudad de Rio Branco, para que el producto vendido sea de buena calidad y no cause daño a la salud de quien lo consume.(AU)

Accuracy of Two Visual Criteria for the Assessment of Caries around Restorations: A Delayed-Type Cross-Sectional Study.

The aim of this study was to compare the performance of two visual criteria used for the detection of caries around restorations in permanent teeth. In this delayed-type cross-sectional study, the study sample was randomly allocated to one of two visual criteria for the assessment of restorations as follows: (a) International Dental Federation (FDI) criteria, considers marginal staining, marginal adaptation, and caries recurrence and (b) Caries Associated with Restorations or Sealants (CARS) criteria, defined by the International Caries Classification and Management System. A calibrated examiner assessed the restorations using two reference standards as follows: (i) for restorations requiring operative interventions (repair/replacement), the restoration was partially or totally removed and the presence or absence of carious tissue was assessed; and (ii) for restorations requiring nonoperative intervention, follow-up for a period of 1 year was recommended to allow identification of the presence of new lesions not detected at baseline. The sensitivity, specificity, area under receiver operating characteristic curve (Az), and accuracy of the criteria were assessed. The study included 305 restorations. The FDI marginal staining parameter had the lowest Az value (Az = 0.501), while similar sensitivity was observed between the CARS (62.0%), FDI presence of caries (65.0%), and FDI marginal adaptation (74.0%) parameters. CARS exhibited the highest specificity (88.3%) and accuracy (85.6%). The CARS criteria exhibited better specificity and accuracy in detecting caries around restorations, followed by the FDI criteria for caries recurrence and marginal adaptation. Considering marginal staining or combining multiple marginal features to assess secondary caries resulted in an increased risk of false-positive outcomes and overtreatment.

A novel approach in public health surveillance: searching the illegal dairy trade in Facebook.

This paper uses the internet-based biosurveillance to describe the Brazilian e-commerce of non-inspected dairy products and maps its traditional sale in a medium-sized city (Londrina). We searched the sales of illegal dairy products on Facebook Marketplace and conducted a census of street markets and a random sample of formal markets. Although prohibited in Brazil, consumers can easily find informal dairy products on internet and retail sale. In addition, 7% of the food products hold a false label to mimic food inspection. The e-commerce was linked with regions with higher access to technology and Human Development Index (HDI). We believe that our approach shows a step forward in the public health interventions and could be replicated stimulating debates about policies required to improve the public health surveillance.

Interventions to Eliminate Cervical Cancer in Nicaragua: An Integrative Review of the Literature.

Introduction: Nicaragua has one of the highest cervical cancer death rates (19.4 per 100,000) compared to other Central American countries. This integrative review of the literature synthesizes cervical cancer prevention interventions in Nicaragua. Methods: The literature search was performed through Cumulative Index to Nursing and Allied Health Literature (CINAHL), Cochrane Library, Embase, PubMed, and Scopus. The six articles included in this integrative review were appraised using the Quality Assessment Tool for Quantitative Studies (QAQTS). Results: Greater knowledge of Pap smears, the human papillomavirus (HPV), and cervical cancer was significantly associated with positive screening beliefs. Barriers to cervical cancer screening included access to Pap smears, preference for female providers, and the length of time to receive screening results and treatment. The probability of having a Pap smear was 94% higher among those who had a recent doctor's visit compared to those who had not. While clinician samples are more accurate in detecting HPV or cervical cancer, self-sampling was stated by the participants to be preferred. Conclusion: A focus on the higher efficacy of clinician sampling and providing more factual information about cervical cancer and its screening through trusted community-based efforts, such as charlas, would be more likely to encourage testing and thereby reduce the rate of cervical cancer in Nicaragua.

Prevalence of bovine fascioliasis and economic losses in an abattoir located in the state of Espírito Santo, Brazil / Prevalência de fasciolose bovina e perdas econômicas em um abatedouro frigorífico localizado no estado do Espírito Santo, Brasil

Bovine fasciolosis is a neglected parasitic zoonosis that causes economic losses to beef production chains due to liver condemnation at abattoirs. Despite the characterization of Bovine fasciolosis in several areas of Brazil, an update on its prevalence in the states of Rio de Janeiro and Espirito Santo is lacking. Thus, fasciolosis prevalence from 2018 to 2021 is presented here according to cattle origin (municipalities of both states). In addition, the associated economic impact on an abbatoir was analyzed through liver condemnation of bovines from both states. The prevalence of fasciolosis in the state of Espírito Santo (10.4%) was significantly higher than Rio de Janeiro (3.6%) (P < 0.01), and resulted in an economic loss of US$12,678.60 for the abattoir. The following municipalities located in the state of Espírito Santo presented prevalences above the mean (10.3%) established in the study: Vargem Alta, Anchieta, Cachoeiro do Itapemirim, Castelo, Atilio Vivacqua, Apiacá and Ibitirama. Thus, majority of these municipalities are considered high-risk areas. Although, there is an aparrent reduction of fasciolosis prevalence, it continues to be a cause of economic losses in abattoirs especially in the state of Espírito Santo. Accordingly, prophylactic measures must still be adopted in cattle farms.

A fasciolose bovina é uma zoonose parasitária negligenciada que resulta em perdas econômicas para a cadeia produtiva da carne devido à condenação de fígado em abatedouros-frigoríficos. Essa doença é descrita em diversas áreas do Brasil, mas existe uma deficiência de informações atuais sobre a prevalência nos estados do Rio de Janeiro e Espírito Santo. Essa nota descreve a prevalência de fasciolose de 2018 a 2021 de acordo com o município de origem e o impacto econômico devido à condenação de fígados em um abatedouro que recebe animais de ambos os estados. A prevalência no Estado do Espírito Santo (10,4%) foi significativamente maior do que no Rio de Janeiro (3,6%) (P < 0,01) e resultou em uma perda econômica de R$ 64.636,00. Os municípios de Vargem Alta, Anchieta, Cachoeiro do Itapemirim, Castelo, Atilio Vivacqua, Apiacá e Ibitirama apresentaram prevalência superior à prevalência desse estudo (10,3%), sendo que esses municípios vem sendo reconhecidos como áreas de alto risco. Apesar de uma aparente redução na prevalência da enfermidade, a fasciolose persiste como uma causa de perdas econômicas aos abatedouros, especialmente no Estado do Espírito Santo, e medidas profiláticas ainda precisam ser adotadas nas fazendas.

Impact on condemnations of bovine carcasses for tuberculosis and cysticercosis after changes in federal legislation in the state of Rio Grande do Sul (2014-2020) / Impacto nas condenações de carcaças bovídeas por tuberculose e cisticercose após as modificações na legislação federal no estado do Rio Grande do Sul (2014-2020)

The inspection of products of animal origin, carried out by veterinary agricultural inspectors, is based on the Brazilian legislation described in the Regulation of Industrial and Sanitary Inspection of Products of Animal Origin of the Ministry of Agriculture, Livestock and Supply. On March 29, 2017, Decree No. 9,013 was published, which repeals Decree No. 30,691, of 1952, with this, major changes occurred in the legislation described in RIISPOA, such as the criteria used for judging carcasses. The objective of this study was to verify whether these changes had an impact on condemnations of bovine carcasses because of cysticercosis and tuberculosis in the State of Rio Grande do Sul. The number of animals slaughtered and the number of carcasses condemned because of tuberculosis and cysticercosis between 2014 and 2020 were obtained from the database of the Agricultural Defense System (SDA), the official data system of the Secretariat of Agriculture, Livestock and Rural Development (SEAPDR), it was possible to observe an increase in the number of cases of carcasses condemned because of cysticercosis and a reduction of animals affected by the disease in the period after the changes in the regulation. The number of carcasses condemned because of tuberculosis, as well as the number of animals affected by the disease, remained stable after the changes in the regulation. The results showed that the changes in federal legislation had a real impact on the historical series of condemnations of bovine carcasses due to cysticercosis in the state of Rio Grande do Sul. In carcasses affected by tuberculosis, the impact of carcass condemnation on the historical series after changes was not significant.

A inspeção de produtos de origem animal, realizada por médicos veterinários fiscais agropecuários, é baseada na legislação brasileira descrita no Regulamento da Inspeção Industrial e Sanitária de Produtos de Origem Animal (RIISPOA) do Ministério da Agricultura, Pecuária e Abastecimento (MAPA). No dia 29 de março de 2017 foi publicado o Decreto nº 9.013, que revoga o Decreto nº 30.691, de 1952, com isso grandes alterações ocorreram na legislação descrita no RIISPOA, como os critérios de julgamento de carcaças. O objetivo desse estudo foi verificar, se essas alterações tiveram impacto nas condenações de carcaças de bovídeos por cisticercose e tuberculose no Estado do Rio Grande do Sul (RS). O número de animais abatidos e o número de carcaças condenadas por tuberculose e cisticercose entre 2014 e 2020 foram obtidos do banco de dados do Sistema de Defesa Agropecuária (SDA), sistema oficial de dados da Secretaria da Agricultura Pecuária e Desenvolvimento Rural (SEAPDR), foi possível observar aumento do número de casos de carcaças condenadas por cisticercose e redução de animais afetados pela enfermidade no período após as alterações no regulamento. O número de carcaças condenadas por tuberculose, assim como o número de animais afetados pela doença se manteve estável após as mudanças no regulamento. Os resultados encontrados mostraram que as modificações na legislação federal trouxeram impacto real na série histórica de condenações de carcaças bovídeas por cisticercose no âmbito do Estado do Rio Grande do Sul enquanto nas carcaças afetadas por tuberculose o impacto das condenações não foi significativo.

Molecular identification of pathogenic Escherichia coli virulence genes and histopathological analysis in chickens condemned for airsacculitis / Identificação molecular de genes de virulência de Escherichia coli e análise histopatológica em frangos condenados por aerossaculite

The present study identified virulence genes and pathological changes caused by Escherichia coli in chicken carcasses condemned for airsacculitis and assessed if the histopathological examination and polymerase chain reaction (PCR) were effective for studies like this. Trachea, liver, and lung were collected from 30 chickens with suspected airsacculitis that has been condemned in the inspection line. The samples were analyzed by PCR to simultaneously identify two virulence genes (iss and tsh genes) and for histopathological testing. PCR efficiently genotypically characterize the E. coli isolates, where the virulence genes iss and tsh were found in three birds simultaneously. The histopathological examination detected a predominance of heterophils and mononuclear cells in the trachea (100%), lung (90%), and liver (13.3%). The liver was the organ where practically no alteration was diagnosed. The results of multiplex PCR for the tsh and iss virulence genes indicate the great potential of the approach in the characterization of E. coli isolates. Unspecific identification did not occur, thus making it necessary to use technologies for the identification and prevention of this agent in aviaries and poultry abattoirs.

O objetivo do presente estudo foi identificar genes de virulência e alterações patológicas provocadas por Escherichia coli em carcaças de frango condenadas por aerossaculite e se o exame histopatológico e uma Reação em Cadeia da Polimerase (PCR) são eficazes para os estudos dessa natureza. Para isso, foram coletados traqueia, fígado e pulmão de 30 frangos condenados na linha de inspeção, com suspeita de aerossaculite. As amostras foram submetidas a PCR para a identificação de dois genes de virulência de modo simultâneo (genes iss e tsh) e ao teste histopatológico. A PCR foi eficiente para caracterizar genotipicamente os isolados de E. coli, em que se constatou em três aves os genes de virulência iss e tsh simultaneamente. No exame histopatológico detectou-se a predominância de heterófilos e mononucleares na traqueia 100%, no pulmão 90% e no fígado 13,3%. O fígado foi o órgão onde praticamente não foi diagnosticada nenhuma alteração. Diante dos resultados obtidos, foi possível observar que a PCR multiplex para os genes de virulência tsh e iss apresenta um grande potencial na caracterização de isolados de E. coli, já que não gerou identificação inespecífica, com isso faz-se necessária a utilização de tecnologias para identificação e prevenção desse agente nos aviários e matadouros avícolas.

"Enxugando Gelo?": o trabalho dos profissionais na fiscalização sanitária de medicamentos na internet / "Rolling a stone uphill?": the work of professionals in health inspection of medicines on the internet

Resumo A sociedade contemporânea convive com incertezas e vulnerabilidades que exigem a atuação dos sistemas regulatórios na fiscalização do comércio eletrônico de medicamentos não registrados e/ou falsificados. Investiga-se o trabalho dos profissionais da vigilância sanitária federal na fiscalização de medicamentos na internet e os elementos que orientam sua decisão técnica. A metodologia utilizada foi a pesquisa qualitativa, do tipo estudo de caso único, ancorada na abordagem ergológica, entre dezembro de 2020 e setembro de 2021, por meio de entrevistas e observação participante. Os mapas ergodialógicos, elaborados no Atlas.ti, subsidiaram a análise das práticas discursivas classificadas conforme esquema tripolar saber-agir-valor, resultando em três categorias. O profissional é sempre convocado a fazer escolhas e gerir situações imprevisíveis, havendo inquietações sobre a capacidade institucional para compreender e agir sobre o problema. O trabalho adquire, assim, o sentido de "enxugar gelo", em função do volume de demandas e dos poucos efeitos da fiscalização no mercado eletrônico de medicamentos. As normas, o conhecimento técnico, a experiência e a responsabilidade social, dentre outros elementos, estão presentes na tomada de decisão dos profissionais, conscientes de que, ao agir, protegem a saúde da população. Constata-se que diferentes saberes, científicos ou empíricos, podem qualificar melhor o sistema decisório na fiscalização, tornando-o mais efetivo, assertivo e transparente.

Abstract Contemporary society is plagued with uncertainties and vulnerabilities, demanding from regulatory systems the surveillance of unregistered and/or falsified medicines sold online. This study investigates the work of federal health surveillance professionals in the inspection of medicines sold online and the elements that guide their technical decision. A qualitative, single case study based on the ergological approach was conducted between December 2020 and September 2021, with data collected by interviews and participant observation. Ergodialogical maps, developed using Atlas.ti, underlined the analysis of discursive practices classified according to the tri-polar knowledge-action-value schema, resulting in three categories. Professionals are always called upon to decide on and manage unpredictable situations, questioning the institutional capacity to understand and act on the problem. The work thus takes on the sense of "rolling a stone uphill" due to the volume of demands and the limited effects of surveillance on the e-commerce of medicines. Norms, technical knowledge, experience, and social responsibility, among other elements, inform the decision-making process of professionals, who are aware that, by acting, they protect public health. Different forms of knowledge, scientific or experiential, can better qualify decision-making in surveillance, making it more effective, assertive, and transparent.

Physicochemical and microbiological quality of refrigerated raw bovine milk commercialized in Presidente Médici, Rondônia state, Western Amazon, Brazil / Qualidade físico-química e microbiológica de leite bovino cru refrigerado comercializado em Presidente Médici, estado de Rondônia, Amazônia Ocidental, Brasil

The aimed of the study was to evaluate the quality of raw milk from some suppliers in rural and urban areas and to test the hypothesis that it is unsafe for direct consumption. The study was conducted with suppliers in rural and urban areas in Presidente Médici municipality, Rondônia state. The method of the Ministério da Agricultura, Pecuária e Abastecimento (MAPA), was used, which consists of titrating a portion of the sample with a sodium hydroxide solution of known concentration, using phenolphthalein as an indicator. According to Brazilian legislation and literature, stability of Alizarol, foreign substances, Chloride, Hypochlorite, Hydrogen Peroxide and Colostrum were analyzed. Microbiological analysis, standard plate count (SPC) and quantification of thermotolerant coliforms were also performed using the most probable number (MPN) technique. Values above the limits established by legislation were found, mainly for temperature and titleable acidity, in addition to stability results against Alizarol and the presence of colostrum. There were high microbial contents with regard to SPC, and, in this case, the presence of pathogenic microorganisms cannot be disregarded. The presence of thermotolerant coliforms may indicate probable contact of milk with feces. Therefore, it is considered that the samples analyzed here are not safe for ingestion in the raw form.(AU)

O objetivo do estudo foi avaliar a qualidade do leite cru de alguns fornecedores da zona rural e urbana e tes-tar a hipótese de que não são seguros para o consumo direto. O estudo foi conduzido em fornecedores da zona rural e urbana do município de Presidente Médici, estado de Rondônia. Empregou-se o método do Ministério da Agricultura Pecuária e Abastecimento (MAPA), que consiste na titulação de uma porção da amostra por uma solução hidróxido de sódio de concentração conhecida, utilizando como indicador a fenolftaleína. Conforme a legislação brasileira e a lite-ratura, foram analisados estabilidade do Alizarol, substâncias estranhas, Cloreto, Hipoclorito, peróxido de Hidrogênio e Colostro. Foram realizadas também análises microbiológicas, contagem Padrão em Placa (CPP) e quantificação dos coliformes termotolerantes através da técnica do número mais provável (NMP). Foram encontrados valores acima dos limites estabelecidos pela legislação, principalmente para temperatura e acidez titulável, além dos resultados de estabili-dade frente ao Alizarol e da presença de colostro. Houve altos teores microbianos no que se refere à CPP, e, nesse caso, a presença de microrganismos patogênicos não pode ser desconsiderada. A presença de coliformes termotolerantes pode indicar provável contato do leite com fezes. Portanto, considera-se que as amostras aqui analisadas não são seguras para ingestão na forma crua.(AU)