Animal pollination shapes fruits market features, seeds functional traits and modulates their chemistry.

In this study, we experimentally addressed the impact of different pollination treatments on the morphological, reproductive and chemical traits of fruits and seeds of two crop species, the wild strawberry (Fragaria vesca L.) and cowpea (Vigna unguiculata (L.) Walp.). Multiple flowers from each plant were exposed to different pollination treatments: (1) self pollination, (2) hand cross pollination and (3) open pollination. Both crops were positively affected by open pollination in terms of morpho-chemical parameters concerning the marketability (e.g., 35% decrease in sugar/acid ratio in open pollinated strawberries compared to the autogamous ones) and the seed germination rate as a proxy of reproduction efficiency (e.g., the almost complete absence of seed abortion in the open pollination treatment). Remarkably, the pollination treatment also strongly influenced the phytochemical composition. Open-pollinated strawberries exhibited a higher relative concentration of compounds endowed with nutraceutical properties such as anthocyanins, ellagic acid derivatives and flavonoids. At the same time, cowpea seeds displayed higher concentrations of anti-nutrients in the self pollination treatments, such as saponins, compared to the open and hand cross pollinated seeds. This study suggests the presence of a link between the pollination mechanism, market quality, plant reproduction and chemical properties of fruits and seeds, supporting the intricate interplay between pollinators, plants and human nutrition, highlighting the crucial importance of animal pollination in the ecological and dietary contexts.

Hydrogen peroxide and salt stress in radish: effects on growth, physiology, and root quality.

Hydrogen peroxide (H2O2) plays a central role in responses to salt stress, a major abiotic stress that impacts crop yield worldwide. Despite the evidence that H2O2 mitigates salt stress and improves post-harvest quality on several species, its effects on radish were not investigated so far. Thus, the objective of this study was to evaluate the exogenous application of H2O2 on salt stress mitigation of radish growth, physiology, and post-harvest quality. For this, radish plants were grown in pots for 30 days, being watered with non-saline (0.31 dS m-1) or saline water (120 mM NaCl, 12.25 dS m-1). Plants were leaf-sprayed weekly with water (control - 0 µM H2O2) or H2O2 (150 or 1500 µM) solutions. The experimental design was completely randomized in a 3 × 2 factorial scheme (H2O2 treatments × salt stress conditions). The growth, physiology (gas exchanges, photochemical efficiency, relative water content, electrolyte leakage, and the contents of chlorophylls and carotenoids), and post-harvest attributes of globular roots (color, anthocyanins, vitamin C, phenolic compounds, and soluble solids) were determined. Salt stress decreased gas exchanges and increased electrolyte leakage, which resulted in stunted radish growth, and increased the contents of antioxidants, such as anthocyanins, soluble solids, and vitamin C, improving globular root quality. Conversely, H2O2 did not mitigate salt stress effects on radish growth, photosynthetic capacity, and oxidative damages. Although H2O2 increased vitamin C under non-stressed condition, it was decreased under salt stress. Thus, we conclude that H2O2 did not mitigate salt stress on radish growth and quality. Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-024-01476-z.

The involvement of Pseudoterranova decipiens fish infestation on the shelf-life of fresh Atlantic cod (Gadus morhua) fillet.

Zoonotic nematodes of the family Anisakidae are highly common in many marine fish species, which act as paratenic hosts for the third larval stage. In the fish, these parasites may migrate from the fish's gastro-intestinal tract (GI-tract) further to the coelomic cavity and muscles, making them a possible contamination source of bacteria they carry on their cuticle and in their GI-tract. A previous study revealed no apparent effect of Anisakis simplex on spoilage of fish, but the equally common anisakid species Pseudoterranova decipiens has a larger body surface potentially increasing the bacterial load brought into the fish muscle upon migration. As the presence of shelf-life reducing spoilage bacteria in the microbiome of this anisakid species has been demonstrated, the objective of the present study was to assess the potential shelf-life reducing effect of P. decipiens in fresh fish fillets stored in a domestic refrigerator. Atlantic cod was used as a model since members of the cod family are the third most consumed marine fish globally and it has the highest prevalence of P. decipiens infections. Infected and non-infected codfish fillet portions were collected and microbiologically analyzed at day 0 and day 4 of storage in a domestic fridge. Three isolation media were used to enhance maximum bacterial recovery and isolates were identified using MALDI-TOF MS and 16S rRNA gene sequencing. In parallel to the microbiological examination, sensory analysis was performed daily on the cod fillets to evaluate the freshness of the fish. Results revealed the presence of typical spoilage bacteria (e.g., Pseudomonas sp., Photobacterium sp.) in all fish, but based on the total viable counts, total H2S-producing bacteria, and sensory analysis, there were no objective indications to assume an increased fish spoilage rate by the presence and migration P. decipiens. Additionally, a beta-diversity comparison revealed no significant differences in microbiota composition between infected and non-infected fish parts, though individual heterogeneity in microbiome composition among Atlantic codfish individuals was found. As total viable counts did, however, exceed the guideline limits for fresh fish, further research should now focus on the role of the candling step as a potential source of post-harvest contamination. As such, anisakid infection might still accelerate fish spoilage, though now in an indirect way.

A new edible coating of fish gelatin incorporated into açaí oil to increase the post-harvest shelf life of tomatoes.

Açaí oil (Euterpe oleracea) is a new active ingredient, originating from the Amazon Forest, which offers numerous benefits as an antioxidant and antimicrobial agent. Here, we report how açaí oil can be used as an active ingredient in gelatin coatings to increase the shelf life of tomatoes. The optimized viscosity and gel strength conditions were 5.40 % gelatin, 17.25 % açaí oil and 18 % plasticizer. FTIR, XRD and zeta potential analysis reveals that repulsive forces dominate the interactions between açaí oil and gelatin. The optimized coating (GAO) reduced mass loss by 8 % and achieved greater firmness (25 N), proving its effectiveness in maintaining tomato quality during storage. For the first time, it was found that the addition of açaí oil to fish gelatin improves the percentage of acidity and firmness of the tomato, delaying ripening, making it a promising alternative as packaging for climacteric fruits.

Effectiveness of aloe vera gel coating and paraffin wax-coated paperboard packaging on post-harvest quality of hog plum (Spondius mangifera L.).

In this research work, hog plum (Spondius mangifera L.) was treated with Aloe vera gel (AVG) coating and paraffin wax-coated box (PWB) packaging and stored at ambient condition (25 ± 3 °C and 80-85% relative humidity) for 12 d to evaluate their impact on postharvest quality and storability. The physicochemical properties, microbiological analysis, and decay evaluation were analyzed throughout the storage period. The AVG and PWB coating treatments both demonstrated a significant effect on the quality of fruits during storage. The results showed that fruits with AVG coating and PWB packaging exhibited lower decay rates, weight loss, color difference, total microbial population, total soluble solids, titratable acidity, and higher fruit firmness and pH than uncoated (control) fruits. The AVG coating was the most effective treatment, followed by the PWB packaging treatment. Our findings show that the AVG coating and PWB packaging treatment can be a promising solution for preserving the quality of hog plums and also helps in increasing the lifetime of hog plums during storage.

Online temperature-monitoring technology for grain storage: a three-dimensional visualization method based on an adaptive neighborhood clustering algorithm.

BACKGROUND: Post-harvest quality assurance is a crucial link between grain production and end users. It is essential to ensure that grain does not deteriorate due to heating during storage. To visualize the temperature distribution of a grain pile, the present study proposed a three-dimensional (3D) temperature field visualization method based on an adaptive neighborhood clustering algorithm (ANCA). The ANCA-based visualization method contains four calculation modules. First, discrete grain temperature data, obtained by sensors, are collected and interpolated using back propagation (BP) neural networks to model the temperature field. Then a new adaptive neighborhood clustering algorithm is applied to divide interpolation data into different categories by combining spatial characteristics and spatiotemporal information. Next, the Quickhull algorithm is used to compute the boundary points of each cluster. Finally, the polyhedrons determined by boundary points are rendered into different colors and are constructed in a 3D temperature model of the grain pile. RESULTS: The experimental results show that ANCA is much better than the DBSCAN and MeanShift algorithms on compactness (around 95.7% of tested cases) and separation (approximately 91.3% of tested cases). Moreover, the ANCA-based visualization method for grain pile temperatures has a shorter rendering time and better visual effects. CONCLUSION: This research provides an efficient 3D visualization method that allows managers of grain depots to obtain temperature field information for bulk grain visually in real time to help them protect grain quality during storage. © 2023 Society of Chemical Industry.

Plant growth regulators preserved the longevity of cut stems of Chrysanthemum morifolium by orchestrating physio-biochemical and anatomical responses.

The marketability of cut flowers is determined by their postharvest quality parameters. Among these parameters, vase life is most crucial and different plant growth regulators (PGRs) play a significant role in regulating vase life. With the objective, to regulate vase life and other quality parameters of commercially important cut stems of chrysanthemum cv. White Star, a study was planned to have an insight into the role of different PGRs in orchestrating underlying physio-biochemical and anatomical responses. Three PGRs viz., Benzyl adenine (50, 100, 150 and 200 µM BA), Thidiazuron (5, 10, 15 and 20 µM TDZ) and Salicylic acid (50, 100, 150 and 200 µM SA) were used as pulsing solution. The results revealed that PGR especially 10 µM TDZ delayed leaf and floret senescence as compared to control. The visual observations (retention of green colour of leaves and white of florets) were correlated to enhanced water absorption, relative water content, membrane stability index, total soluble sugars and total soluble proteins ; higher content of chlorophyll in leaves and lower content of carotenoids and anthocyanins in florets. Further, the antioxidant enzymes (peroxidase and catalase) activities were also higher in PGR-treated stems than in control. These results were further supported by anatomical studies that indicated minimum blockage in xylem and maintenance in turgidity of cells as revealed through the size of tissues (radius of pith) of treated stems (control-118.48 mm and 10 µM TDZ-177.94 mm). Although all PGR concentrations significantly maintained relative water content, membrane stability index, respiratory substrates and antioxidant activity for a longer time, the low concentrations of TDZ had the most relevant impact on longevity. The longevity of stems treated with10 µM TDZ was up to 23 days as compared to 13 days in control. So 10 µM TDZ could be used as a chemical tool to improve chrysanthemum longevity and increase its commercial potential.

Artificial intelligence-based prediction of lycopene content in raw tomatoes using physicochemical attributes.

INTRODUCTION: Lycopene consumption reduces risk and incidence of cancer and cardiovascular diseases. Tomatoes are a rich source of phytochemical compounds including lycopene as a major constituent. Lycopene estimation using high-performance liquid chromatography is time-consuming and expensive. OBJECTIVE: To develop artificial intelligence models for prediction of lycopene in raw tomatoes using 14 different physicochemical parameters including salinity, total dissolved solids (TDS), electrical conductivity (EC), firmness, pH, total soluble solids (TSS), titratable acidity (TA), colour values on Hunter scale (L, a, b), total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity (AOA). MATERIAL AND METHODS: The post-harvest data acquisition was collected through investigation for more than 100 raw tomatoes stored for 15 days. Linear multivariate regression (LMVR), principal component regression (PCR) and partial least squares regression (PLSR) models were developed by splitting data set into train and test datasets. The training of models was performed using 10-fold cross validation (CV). RESULTS: Principal component analysis showed strong positive association between lycopene, colour value 'a', TPC, TFC and AOA. The R2 (CV), root mean square error (RMSE) (CV) and RMSE (Test) for best LMVR model was observed to be at 0.70, 8.48 and 9.69 respectively. The PCR model revealed R2 (CV) at 0.59, RMSE (CV) at 8.91 and RMSE (Test) at 10.17 while PLSR model revealed R2 (CV) at 0.60, RMSE (CV) at 9.10 and RMSE (Test) at 10.11. CONCLUSION: Results of the present study show that epidemiological studies suggest fully ripened tomatoes are most beneficial for consumption to ensure recommended daily intake of lycopene content.

Post-Harvest LED Light Irradiation Affects Firmness, Bioactive Substances, and Amino Acid Compositions in Chili Pepper (Capsicum annum L.).

Chili pepper is an important vegetable and spice crop with high post-harvest deteriorations in terms of commercial and nutritional quality. Light-emitting diodes (LEDs) are eco-friendly light sources with various light spectra that have been demonstrated to improve the shelf-life of various vegetables by manipulating light quality; however, little is known about their effects on the post-harvest nutritional quality of chili peppers. This study investigated the effects of different LED lightings on the post-harvest firmness and nutritional quality of chili peppers. We found that red and blue light could increase the content of capsaicinoids, whereas white and red light could increase the essential and aromatic amino acid (AA) content in pepper. Nonetheless, the influence of light treatments on AA contents and compositions depends strongly on the pepper genotype, which was reflected by total AA content, single AA content, essential AA ratio, delicious AA ratio, etc., that change under different light treatments. Additionally, light affected fruit firmness and the content of nutrients such as chlorophyll, vitamin C, and total carotenoids, to varying degrees, depending on pepper genotypes. Thus, our findings indicate that LED-light irradiation is an efficient and promising strategy for preserving or improving the post-harvest commercial and nutritional quality of pepper fruit.

Productive and Physico-Chemical Parameters of Tomato Fruits Submitted to Fertigation Doses with Water Treated with Very Low-Frequency Electromagnetic Resonance Fields.

It is known that poorly performed fertigation directly impacts on tomato production and biometric components. In addition, consumers are also affected by interrelated characteristics that interfere with the acceptability of the fruit, such as the physicochemical parameters and nutrients in the fruit. Thus, eco-friendly technologies, such as irrigation with ultra-low frequency electromagnetic treated-water, which attenuates the inadequate management of fertigation, are essential to improve marketable fruit yields. Thus, the objective of the present work was to investigate the impact of treated water with very low-frequency electromagnetic resonance fields in physical, chemical and nutritional parameters at different nutrient solution strengths in tomato fruits. In this study, experiments were carried out in randomized blocks and five doses of fertigation were used (1.5; 2.5; 4.0; 5.5; and 7.0 dS m−1), employing two types of water: electromagnetically treated and untreated. It can be seen that the fertigation affected some parameters, mainly the number of fruits with blossom-end rot, fruit size, and weight. Variance analysis (ANOVA) was performed with the subsequent use of the Tukey test. In all statistical tests, a confidence level of 95% was considered. The soluble solids content increased by 28% as a function of the fertigation doses. The electromagnetically treated water reduced the number of fruits with blossom-end rot by 35% (p < 0.05). Overall, electromagnetic water improved the physicochemical quality parameters and the nutritional status of tomato fruits. Thus, this study demonstrated that green technology could leverage tomato fruit production and quality.

Melatonin as a plant biostimulant in crops and during post-harvest: a new approach is needed.

A great amount of data covering a wide variety of plant species and experimental conditions has demonstrated the beneficial actions that melatonin exerts on many aspects of plant development, including germination, photosynthesis and water economy. Melatonin behaves especially well as a plant biostimulator against biotic and abiotic stressors, increasing stress tolerance. The present contribution sets out possible future multidisciplinary studies, in which the impact of using melatonin with respect to agriculture, food technology, human nutrition and the environment needs to be clearly established. In crops, the effective dose and best formulations for individual plant species and cultivation conditions should be studied. As regards post-harvest, the focus should be on the half-life time of melatonin in fruits and water-residue treatments. Detailed studies are lacking on the human intake of phytomelatonin in different diets. Studies on the metabolization of phytomelatonin and the combined effect with other phytonutrients such as carotenoids, chlorophylls, flavonoids, fibers, etc., would also be of interest. In soils, the possible interaction between melatonin and microbiome and non-vertebrate animals is of primordial interest. In terms of the environment, although melatonin is classified as a non-hazardous agent, its limitations as a possible animal hormone disruptor have been suggested. Specific studies on the permanence of melatonin in plant tissues, plant by-products, soil, freshwater and honeybees, amongst others, are proposed to obtain crucial information. © 2021 Society of Chemical Industry.

Long-term storage effect on chemical composition, nutritional value and quality of Greek onion landrace "Vatikiotiko".

The effect of storage at two temperatures (5±1 and 25±1 °C and 60-70±5% RH for both temperatures) on marketability and quality features of dry bulbs of local landrace "Vatikiotiko", "Sivan F1", "Red Cross F1" and "Creamgold" was examined. During storage measurements for fresh and dry weight of bulbs, tunic and flesh color, bulb firmness, nutritional value and mineral composition were taken at regular intervals. Storage concluded when either bulbs lost marketable quality or sprouting occurred. "Vatikiotiko" onion can be stored for at least 7months at 25±1 °C, whereas at 5±1 °C storage could be prolonged without significant marketability and quality loss. The fact that "Vatikiotiko" landrace can be considered a "storage" onion has to be capitalized in order to increase total production and yield, since storage could cover the market needs that arise throughout the year.

Bee pollination improves crop quality, shelf life and commercial value.

Pollination improves the yield of most crop species and contributes to one-third of global crop production, but comprehensive benefits including crop quality are still unknown. Hence, pollination is underestimated by international policies, which is particularly alarming in times of agricultural intensification and diminishing pollination services. In this study, exclusion experiments with strawberries showed bee pollination to improve fruit quality, quantity and market value compared with wind and self-pollination. Bee-pollinated fruits were heavier, had less malformations and reached higher commercial grades. They had increased redness and reduced sugar-acid-ratios and were firmer, thus improving the commercially important shelf life. Longer shelf life reduced fruit loss by at least 11%. This is accounting for 0.32 billion US$ of the 1.44 billion US$ provided by bee pollination to the total value of 2.90 billion US$ made with strawberry selling in the European Union 2009. The fruit quality and yield effects are driven by the pollination-mediated production of hormonal growth regulators, which occur in several pollination-dependent crops. Thus, our comprehensive findings should be transferable to a wide range of crops and demonstrate bee pollination to be a hitherto underestimated but vital and economically important determinant of fruit quality.