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1.
J Environ Sci (China) ; 148: 541-552, 2025 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-39095187

RESUMO

The ocean serves as a repository for various types of artificial nanoparticles. Nanoplastics (NPs) and nano zinc oxide (nZnO), which are frequently employed in personal care products and food packaging materials, are likely simultaneously released and eventually into the ocean with surface runoff. Therefore, their mutual influence and shared destiny in marine environment cannot be ignored. This study examined how nanomaterials interacted and transported through sea sand in various salinity conditions. Results showed that NPs remained dispersed in brine, while nZnO formed homoaggregates. In seawater of 35 practical salinity units (PSU), nZnO formed heteroaggregates with NPs, inhibiting NPs mobility and decreasing the recovered mass percentage (Meff) from 24.52% to 12.65%. In 3.5 PSU brackish water, nZnO did not significantly aggregate with NPs, and thus barely affected their mobility. However, NPs greatly enhanced nZnO transport with Meff increasing from 14.20% to 25.08%, attributed to the carrier effect of higher mobility NPs. Cotransport from brackish water to seawater was simulated in salinity change experiments and revealed a critical salinity threshold of 10.4 PSU, below which the mobility of NPs was not affected by coexisting nZnO and above which nZnO strongly inhibited NP transport. This study highlights the importance of considering the mutual influence and shared destiny of artificial nanoparticles in the marine environment and how their interaction and cotransport are dependent on changes in seawater salinity.


Assuntos
Águas Salinas , Salinidade , Água do Mar , Poluentes Químicos da Água , Óxido de Zinco , Óxido de Zinco/química , Água do Mar/química , Águas Salinas/química , Poluentes Químicos da Água/química , Poluentes Químicos da Água/análise , Porosidade , Microplásticos , Modelos Químicos , Nanopartículas Metálicas/química
2.
Zookeys ; 1214: 59-75, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39391537

RESUMO

Limnichthyskoreanus sp. nov. is described on the basis of the holotype and 11 paratypes from subtidal waters of Seogwipo, Jeju Island, Korea. The new species had previously been regarded as the Northern Hemisphere population of the anti-equatorial L.fasciatus, but molecular analyses of mitochondrial COI and 16S genes recovers deep genetic divergences of 9.4% and 15.0% between the new species and topotypical specimens of L.fasciatus. Limnichthyskoreanus sp. nov. is distinguished from all other species of Limnichthys based on the following combination of colouration and morphological characteristics: 38-40 vertebrae; 0-6 dorsal saddles joining mid-lateral stripe; small infraorbital sensory pores; a single median interorbital pore; and well-developed vomerine teeth. Summary characters for comparative congeneric species are provided.

3.
Sci Rep ; 14(1): 23364, 2024 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-39375482

RESUMO

A pile group under lateral loading is a complex pile‒soil-pile interaction problem. In this study, numerical analysis was used to investigate the lateral behavior of pile groups arranged in a 3 × 3 configuration with varying piles spacings. The hypoplastic constitutive model embedded into ABAQUS software through the UMAT subroutine was used to characterize the stress‒strain relationship of Toyoura sand. The effects of the pile spacing on the load‒displacement relationship of the pile group, the bending moment-depth relationship of each pile in the pile group, the deflection-depth relationship, and the soil resistance distribution were investigated. The results indicate that the lateral bearing capacity of the pile group decreases by more than 50% when the pile spacing is reduced from 7D to 3D. When the pile spacing is greater than 5D, the "edge effect" of the laterally loaded pile group gradually decreases, and when the pile spacing is greater than 7D, the "shadowing effect" can be negligible, and the wedge-shaped failure zone of the pile group occurs within a depth of 5D below the mud surface.

4.
Artigo em Inglês | MEDLINE | ID: mdl-39367219

RESUMO

A ubiquitous distribution of plastic debris has been reported in aquatic and terrestrial environments; however, the interactions between plastics and radionuclides and the radioactivity of environmental plastics remain largely unknown. Here, we characterize biofilms developing on the surface of plastic debris to explore the role of plastic-associated biofilms as an interaction medium between plastics and radiocesium (137Cs) in the environment. Biofilm samples were extracted from plastics (1-50 mm in size) collected from two contrasting coastal areas in Japan. The radioactivity of plastics was estimated based on the 137Cs activity concentration of the biofilms and compared seasonally with surrounding environmental samples (i.e., sediment and sand). 137Cs traces were detected in biofilms with activity concentrations of 21-1300 Bq·kg-1 biofilm (dry weight), corresponding to 0.04-4.5 Bq·kg-1 plastic (dry weight). Our results reveal the interaction between 137Cs and plastics and provide evidence that organic and mineral components in biofilms are essential in 137Cs retention in environmental plastics. Given the ubiquitous distribution of plastic debris in the environment, more attention should be directed to bioaccumulation and the radioecological impacts of plastic-associated radionuclides on ecosystems.

5.
Water Res ; 267: 122517, 2024 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-39353344

RESUMO

Iron (Fe2+), manganese (Mn2+), and ammonium (NH4+) oxidation processes were studied in three single media and three dual media full-scale rapid sand filters (RSFs) using reactive transport modelling (RTM) in PHREEQC and parameter estimation using PEST. Here, we present the insights gained into the spatial distribution of Fe and Mn mineral coatings in RSFs and its influence on the oxidation sequence and rates. Fe2+ and Mn2+ oxidation predominantly occurred simultaneously in the RSFs, contrary to the expected sequential oxidation based on Gibbs free energy calculations. During backwashing, RSF grains become fully mixed, which initiates heterogeneous Mn2+ oxidation on Mn-coated grains that end up in the top layer. The resulting grains have a mixed Fe/Mn mineral coating, which is limiting heterogeneous Mn2+ oxidation due to the limited Mn mineral surface available. Mixed coatings did not seem to affect Fe2+ oxidation rates, instead oxidation rates were increasing at lower pH. We found that RSFs can be designed to spatially separate Fe2+ and Mn2+ oxidation, which results in optimal conditions for Mn2+ oxidation. The RSF needs to consist of two layers with varying density to inhibit mixing and complete Fe2+ oxidation should occur in the top layer. The developed RTM can be used to estimate the depth at which Fe2+ oxidation is complete, and thus the ideal intersection depth of the two layers. A novel perspective is provided on how mineral coating distribution in single and dual media filters influence removal rates and the sequence of oxidation, which contributes to the design of more efficient groundwater filters.

6.
Biofouling ; : 1-12, 2024 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-39319546

RESUMO

The purpose of this study was to investigate dynamics of biofilm biomass on microparticles of natural material quartz sand and the artificial material polypropylene (plastisphere) as well as change in biofilm-forming microorganisms' number under a short-term in situ field study. In this study microparticles of polypropylene and quartz sand ranging in size from 3 to 5 mm were used. The total microbial count and the number of sulfate-reducing bacteria in the biofilm (by traditional culture-based microbiological methods) and the biofilm biomass (by the method with the crystal violet) were investigated. According to the determined microbiological indicators, over time (90 days) on the polypropylene it was observed decreasing of both the number of studied groups of microorganisms and the formation of a microbial biofilm, compared to the quartz sand. Determination of microbiological indicators of the materials surface allows understanding the aspects of their preservation/removal from the environment and requires additional research.

7.
Materials (Basel) ; 17(18)2024 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-39336244

RESUMO

Biomass bottom ash (BBA) is a by-product of the energy industry and is produced from biomass-fired thermal power plants. They represent the coarsest fraction of the recovered ash and are mostly landfilled. Several researchers have investigated the feasibility of the use of BBA as a replacement for natural aggregates in cementitious material. The utilisation of BBA in the manufacturing of concrete provides an economic and ecological way to upcycle it. At the same time, its use conserves natural resources and promotes sustainability. This review article first presents the chemical, mineralogical and physical properties of BBA, to highlight the possible effects on cementitious materials and the interest in valorising them as a building material. Secondly, the focus is on the utilisation of BBA incorporated in place of natural aggregates used in the manufacturing of concrete. This review investigates the multi-physical properties of concrete manufactured with the partial incorporation of BBA. This substitution leads to decreased workability, which can be limited by the use of admixtures. In the hardened state, a reduction in the mechanical properties is shown with BBA replacement. However, many experimental works show that BBA can be used in appropriate proportions to maintain the specified properties of the concrete.

8.
Materials (Basel) ; 17(18)2024 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-39336310

RESUMO

The development of self-compacting lightweight concretes is associated with solving two conflicting tasks: achieving a structure with both high flowability and homogeneity. This study aimed to identify the technological and rheological characteristics of the flow of concrete mixtures D1400…D1600 based on hollow microspheres in comparison with heavy fine-grained D2200 concrete and to establish their structural and physico-mechanical characteristics. The study of the concrete mixtures was carried out using the slump flow test and the rotational viscometry method. The physical and mechanical properties were studied using standard methods for determining average density and flexural and compressive strength. According to the results of the research conducted, differences in the flow behaviors of concrete mixtures on dense and hollow aggregates were found. Lightweight concretes on hollow microspheres exhibited better mobility than heavy concretes. It was shown that the self-compacting coefficients of the lightweight D1400...D1600 concrete mixtures were comparable with that of the heavy D2200 concrete. The rheological curves described by the Ostwald-de Waele equation showed a dilatant flow behavior of the D1400 concrete mixtures, regardless of the ratio of quartz powder to fractionated sand. For D1500 and D1600, the dilatant flow behavior changed to pseudoplastic, with a ratio of quartz powder to fractional sand of 25/75. The studied compositions of lightweight concrete can be described as homogeneous at any ratio of quartz powder to fractional sand. It was shown that concrete mixtures with a pronounced dilatant flow character had higher resistance to segregation. The value of the ratio of quartz powder to fractional sand had a statistically insignificant effect on the average density of the studied concretes. However, the flexural and compressive strengths varied significantly more in heavy concretes (up to 38%) than in lightweight concretes (up to 18%) when this factor was varied. The specific strength of lightweight and heavy concrete compositions with a ratio of quartz powder to fractional sand of 0/100 had close values in the range of 20.4...22.9 MPa, and increasing the share of quartz powder increased the difference between compositions of different densities.

9.
Parasit Vectors ; 17(1): 388, 2024 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-39267125

RESUMO

BACKGROUND: Western Ghats is a biodiversity treasure trove with reports of indigenous leishmaniasis cases. Hence, systematic sand fly surveillance was carried out among the tribal population. The present study reports a novel sand fly species, Phlebotomus (Anaphlebotomus) ajithii n. sp. (Diptera: Psychodidae), discovered in the Western Ghats of India. METHODS: A comprehensive sand fly survey was conducted across the Kollam, Thrissur, Idukki, Kasaragod and Malappuram districts of Kerala, India. The survey spanned both indoor and outdoor habitats using standard collection methods over a 3-year, 3-month period. DNA barcoding of samples was performed targeting mitochondrial cytochrome c oxidase subunit I (COI) gene, and the sequence generated was subjected to phylogenetic analysis. RESULTS: Phlebotomus (Anaphlebotomus) ajithii, a new sand fly species, is recorded and described in this communication. The morphological relationship of the new species to other members of the subgenus Anaphlebotomus is discussed. Mitochondrial COI barcode followed by phylogenetic analysis confirmed that specimens of Ph. ajithii belong to the same taxonomic group, while a genetic distance of 11.7% from congeners established it as a distinct species. CONCLUSIONS: The Western Ghats, known for its rich biodiversity, has lacked systematic entomological surveys focusing on sand flies. This study aims to fill this gap and reports and describes a new species of sand fly.


Assuntos
Complexo IV da Cadeia de Transporte de Elétrons , Phlebotomus , Filogenia , Animais , Índia , Phlebotomus/genética , Phlebotomus/classificação , Complexo IV da Cadeia de Transporte de Elétrons/genética , Código de Barras de DNA Taxonômico , Feminino , Masculino
10.
Materials (Basel) ; 17(17)2024 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-39274652

RESUMO

This paper mainly explores the feasibility of using desert sand (DS) and recycled aggregate in cement-stabilized bases. Recycled coarse aggregate (RCA) and DS serve as the substitutes of natural coarse and fine aggregates, respectively, in cement-stabilized bases. A four-factor and four-level orthogonal test is designed to analyze the unconfined compressive strength, splitting tensile strength, and compressive resilient modulus. Furthermore, this paper investigates the effects of cement content, fly ash (FA) replacement rate, RCA replacement rate, and DS replacement rate on the road performance of cement-stabilized bases composed of RCA and DS. The test results reveal that the performance of cement-stabilized bases with partial RCA instead of natural coarse aggregate (NCA) and partial DS instead of natural fine aggregate satisfies the road use. The correlation and microscopic analyses of the test results imply the feasibility of applying DS and recycled aggregate to cement-stabilized bases. This paper calculates and evaluates the life cycle of carbon emissions of desert sand and recycled coarse aggregate cement-stabilized macadam (DRCSM) and finds that both DS and RCA can reduce the carbon emissions of CSM, which has a positive effect on improving the environment and solving the climate crisis. It is hoped that this paper can offer a solid theoretical foundation for promoting the application of DS and recycled aggregate in road engineering.

11.
Materials (Basel) ; 17(17)2024 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-39274697

RESUMO

This study investigates the potential use of Yellow River sand (YRS) sourced from the lower reaches of the Yellow River in China as a sustainable and cost-effective substitute for quartz sand in Engineered Cementitious Composites (ECC). With an annual accumulation of approximately 400 million tons in this region, YRS presents a substantial resource. ECC specimens with 100% YRS replacement with quartz sand were subjected to various curing methods: natural, steam, standard, and sprinkler. Extensive mechanical testing including flexural, compressive, uniaxial tensile, and four-point flexural tests was conducted. Additionally, Scanning Electron Microscope (SEM) and Mercury Intrusion Porosimetry (MIP) analyses investigated microscopic mechanisms influencing macroscopic mechanical properties. Finally, the mechanical properties of the YRS-ECC test block after 14 days of standard curing and the traditional sand ECC test block were compared and analyzed. The results indicate that ECC specimens with 100% YRS substitution under natural curing show an optimal ultimate tensile strain of more than 4%, providing the best resistance to the reduction in ultimate flexural load and deflection due to aging. Steam curing enhances flexural and compressive strength, achieving an ultimate flexural load of 5 kN and a maximum deflection of 4.42 mm at 90 days. SEM analysis revealed lower C-S-H gel density under natural curing and higher under steam curing, enhancing fiber pull-out in steam-cured specimens. The MIP tests demonstrated that natural curing had the highest porosity (32.86%) and average pore size (51.69 nm), whereas steam curing resulted in the smallest average pore size, with 44% of pores under 50 nm. Compared with traditional sand, it is found that the ultimate bending load and deflection of YRS-ECC are 5.7% and 9.4% higher than those of traditional sand ECC, respectively, and its ultimate tensile strength and strain are also improved. These findings highlight YRS as a sustainable alternative to natural sand in ECC, with natural curing proving the most effective for superior mechanical performance, including tensile strain, crack resistance, and durability.

12.
Environ Pollut ; 361: 124853, 2024 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-39218197

RESUMO

The effectiveness of bioretention cells for heavy metals (HMs) and microplastics (MPs) removal from stormwater runoff has been demonstrated. Knowledge of the mechanisms that dictate the interactions between MPs and HMs would be helpful in pollution control. In this study, the performances of different water-soil-plant bioretention cells for HMs removal through the interception of polyethylene MPs (PE-MPs) were investigated. The results showed that PE-MPs bound to HMs and preferentially tended to bind to Pb (32%-44%) in the complex HMs (Cu, Zn, Cd, and Pb). This could be the reason that the concentration of Pb significantly increased in the effluent under low-intensity simulated rainfall events over a long duration. The accumulation of 1.49 g/kg PE-MPs caused a significant soil pH value decrease and a notable soil zeta potential increase in the bioretention cell, while the low sand/silt ratio media buffered this process. The retention of PE-MPs increased 138.5% in the 0-10 cm soil surface layer when the sand/silt ratio reduced from 2:1 to 1:1 and planted with Canna indica. Meanwhile, PE-MPs amplified the instability of Zn removal in bioretention cells under low-intensity rainfall events in long-duration, high silt percentage substrate and vegetation coverage. The study would contribute to developing a long-term management program for PE-MPs and HMs trapped in bioretention cells to reduce the risk of pollution transport.

13.
Emerg Infect Dis ; 30(10): 2099-2107, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39320166

RESUMO

Bartonella spp. are opportunistic, vectorborne bacteria that can cause disease in both animals and humans. We investigated the molecular occurrence of Bartonella spp. in 634 phlebotomine sand fly specimens, belonging to 44 different sand fly species, sampled during 2017-2021 in north and northeastern Brazil. We detected Bartonella sp. DNA in 8.7% (55/634) of the specimens by using a quantitative real-time PCR targeting the 16S-23S internal transcribed spacer intergenic region. Phylogenetic analysis positioned the Lutzomyia longipalpis sand fly-associated Bartonella gltA gene sequence in the same subclade as Bartonella ancashensis sequences and revealed a Bartonella sp. sequence in a Dampfomyia beltrani sand fly from Mexico. We amplified a bat-associated Bartonella nuoG sequence from a specimen of Nyssomyia antunesi sand fly. Our findings document the presence of Bartonella DNA in sand flies from Brazil, suggesting possible involvement of these insects in the epidemiologic cycle of Bartonella species.


Assuntos
Infecções por Bartonella , Bartonella , Insetos Vetores , Filogenia , Psychodidae , Animais , Bartonella/genética , Bartonella/isolamento & purificação , Bartonella/classificação , Brasil/epidemiologia , Psychodidae/microbiologia , Insetos Vetores/microbiologia , Infecções por Bartonella/microbiologia , Infecções por Bartonella/epidemiologia , Infecções por Bartonella/transmissão , DNA Bacteriano/genética
14.
Front Zool ; 21(1): 25, 2024 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-39343896

RESUMO

Toe fringes are a key innovation for sand dwelling lizards, and the relationship between toe fringe function and substrate properties is helpful in understanding the adaptation of lizards to sand dune environments. We tested the sand burial performance of Phrynocephalus mystaceus on different sand substrates with toe fringe manipulation, with the aim of assessing whether the function of the toe fringes shifts under different substrate properties, especially in highly mobile substrates. The sand burial performance of P. mystaceus was influenced by substrate properties in relation to the toe fringe states of the lizard. After removal of the bilateral toe fringes, the sand burial ability score of P. mystaceus was significantly higher on sand substrates below 100 mesh than on native sand substrates. As the angle of stability of the substrate properties decreased, the sand burial performance of the lizard was even better after the bilateral toe fringes were removed. The results of the LASSO model and the path analysis model showed that the stability angle provided the opposite effect on sand burial performance in different toe fringe states. These results further suggest that the sand burial function of toe fringes may not be suitable for highly mobile sand substrates. It remains to be tested further whether the function of toe fringes is more important for running on sand.

15.
Sci Rep ; 14(1): 22745, 2024 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-39349566

RESUMO

The cementation of desert aeolian sand is a key method to control land desertification and dust storms, so an economical, green and durable process to reach the binding between sand grains needs to be searched. The method based on the microbially induced calcite precipitation (MICP) appeared in recent years as a promising process that proved its efficiency. The feasibility of the MICP technique to treat aeolian sand composed by low clay content, fine particles, low water content and characterized by weak permeability was demonstrated in the present paper. The effects of initial dry density, cementation number and curing time on the permeability and strength of MICP-treated aeolian sand were investigated using permeability tests and unconfined compressive strength (UCS) tests. The microstructure of aeolian sand was observed by scanning electron microscopy (SEM) tests and X-ray diffraction (XRD), aiming to reveal the solidification principle of MICP. The tests result indicated that when the initial dry density and the cementation number rose, the hydraulic conductivity of aeolian sand decreased while the mechanical strength given by UCS values improved. When the initial dry density was 1.65 g/cm3, the curing time was 3 h and the cementation number reached 20, the hydraulic conductivity and UCS reached 0.00151 cm/s and 1050.30 kPa, respectively. With increasing curing time, the hydraulic conductivity first decreased, followed by an increase, while the UCS exhibited an up and then a downtrend. Furthermore, the correlation between UCS values and the CaCO3 content reached a high R2 value equal to 0.912, which confirmed that the cementation occurred in sandy material and governed the soil strengthening. Indeed, the calcium carbonate crystals observed by SEM and XRD enhanced the friction between particles when they wrapped around the sand grains surface, while carbonates reduced the soil permeability when filling the pores and sticking the sand particles together. Finally, the theoretical and scientific knowledge brought by the present study should help in managing sand in desert areas.

16.
Trends Parasitol ; 40(10): 876-885, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39218719

RESUMO

Leishmania make an abundant glycoprotein and proteophosphoglycan-rich gel, called the promastigote secretory gel, in the anterior midgut of their sand fly vector. This gel is a multi-faceted virulence factor which promotes the survival and transmission of the parasites between hosts. Here, we present the case that Leishmania parasites embedded in the promastigote secretory gel should be redefined as a biofilm as it shares striking similarities in biogenesis, form, and function with biofilms of other unicellular organisms. We believe that this reinterpretation will stimulate new hypotheses and avenues of research to improve our understanding of the developmental programme of Leishmania and the interaction these parasites and other kinetoplastids have with their insect hosts.


Assuntos
Biofilmes , Leishmania , Biofilmes/crescimento & desenvolvimento , Leishmania/fisiologia , Animais , Leishmaniose/transmissão , Leishmaniose/parasitologia , Interações Hospedeiro-Parasita/fisiologia , Psychodidae/parasitologia , Psychodidae/microbiologia , Proteínas de Protozoários/metabolismo , Humanos
17.
J Hazard Mater ; 479: 135766, 2024 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-39244984

RESUMO

Nanoplastics (NPs) pose great challenges to soil-groundwater systems. This study investigated the transport and retention of self-synthesized 0.5-µm polystyrene NPs with different shapes using column experiments. The regular NPs were with spherical shapes, while the irregular NPs were with toroid-like shapes. The toroid-like shapes were the irregular shapes (with low aspect ratio) which have not been studied yet. The explorations were carried out in both 5-25 mM NaNO3 and 1-10 mM Ca(NO3)2 solutions. Both breakthrough curves (BTCs) and retained profiles (RPs) were monitored. Our findings uncovered a clear disparity in the transport of irregular and regular NPs, with irregular particles exhibiting lower transport ability compared to the regular ones. For example, the average breakthrough plateaus of the regular and irregular NPs were ∼0.9 and ∼0.5, respectively, in 10 mM NaNO3. In-depth theoretical analysis indicated that the lower XDLVO interaction energy barrier between the irregular NPs and quartz sand was one factor, and the greater margination of irregular NPs on quartz sand, as verified by the numerical simulation, was another factor leading to the decreased transport and increased retention of the irregular NPs. The obtained results highlighted the significance of considering particle shape in future modelling and predicting the fate of NPs in real environmental circumstances.

18.
Biotechnol Rep (Amst) ; 44: e00857, 2024 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-39328926

RESUMO

Characterization of the temperature effects on the abundance and richness of the microbiota of Lutzomyia longipalpis, insect vector of Leishmania infantum in America, is an aspect of pivotal importance to understand the interactions between temperature, bacteria, and Leishmania infection. We developed and used a customized device with a temperature gradient (21-34 °C) to assess the temperature preferences of wild females of Lu. longipalpis collected in a rural area (Ricaurte, Cundinamarca, Colombia). Each replicate consisted of 50 females exposed to the gradient for an hour. At the end of the exposure time, insects were collected and separated by the temperature ranges selected varying from 21 °C to 34 °C. They were organized in 17 pools from which total DNA extracts were obtained, and samples were subjected to 16S rRNA amplicon sequencing analyzes. The most abundant phyla across the different temperature ranges were Proteobacteria (17.22-90.73 %), Firmicutes (5.99-77.21 %) and Actinobacteria (1.56-59.85 %). Results also showed an abundance (30 % to 57.36 %) of Pseudomonas (mainly at temperatures of 21-29 °C and 34 °C) that decreased to 6.55 %-13.20 % at temperatures of 31-33 °C, while Bacillus increase its abundance to 67.24 % at 29-33 °C. Serratia also had a greater representation (49.79 %), specifically in sand flies recovered at 25-27 °C. No significant differences were found at α-diversity level when comparing richness using the Shannon-Wiener, Simpson, and Chao1 indices, while ß-diversity differences were found using the Bray-Curtis index (F-value of 3.5073, p-value < 0.013, R-squared of 0,4889), especially in the groups of Lu. longipalpis associated at higher temperatures (29-33 °C). It was also possible to detect the presence of endosymbionts such as Spiroplasma and Arsenophonus in the range of 29-33 °C. Rickettsia was only detected in Lu. longipalpis sand flies recovered between 25-27 °C. It was possible to characterize Lu. longipalpis microbiota in response to intraspecific temperature preferences and observe changes in bacterial communities and endosymbionts at different ranges of said environmental variable, which may be important in its vector competence and environmental plasticity to adapt to new climate change scenarios.

19.
Parasite ; 31: 57, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39331804

RESUMO

Blood-feeding arthropods are involved in the transmission of several pathogens that have a major impact on public health. Entomological investigations highlighted the composition, abundance, and diversity of flying hematophagous arthropods at four dog shelters located in central Morocco during an eight-month study, with the aim of discussing their vectorial roles and assessing the risk of these shelters as foci for zoonotic diseases. Monitoring of the arthropod fauna for 64 catch nights resulted in the collection of 2,321 biting midges (Ceratopogonidae), 570 mosquitoes (Culicidae), and 475 sand flies (Psychodidae). Fourteen Culicoides species were recorded and dominant species were Culicoides imicola (55.96%), C. paolae (16.07%), C. circumscriptus (10.29%), and C. newsteadi (5.77%). Three mosquito species were collected, including Culex pipiens s.l. (96.84%), Culiseta longiareolata (2.80%), and Cx. perexiguus (0.36%). Ten sand fly species were collected, including seven Phlebotomus species (62.70%) and three Sergentomyia species (37.30%); Sergentomyia minuta was the most dominant species (34.31%), followed by Phlebotomus sergenti (32.42%), typical Ph. perniciosus (8.63%), Ph. alexandri (6.94%), and Ph. riouxi (6.52%). The coexistence of several vectors in these study areas indicates the potential circulation of a wide range of pathogens, including zoonotic ones, thus requiring the implementation of surveillance and control programs to prevent the emergence and spread of disease outbreaks.


Title: Diversité des culicoïdes, moustiques et phlébotomes collectés au niveau de quatre chenils du Maroc central. Abstract: Les arthropodes hématophages sont impliqués dans la transmission de plusieurs agents pathogènes ayant un impact majeur sur la santé publique. Des investigations entomologiques ont mis en évidence la composition, l'abondance et la diversité des arthropodes hématophages volants dans quatre chenils situés au centre du Maroc au cours d'une étude de huit mois, dans le but de discuter de leurs rôles vectoriels et d'évaluer le risque de ces chenils comme foyers de maladies zoonotiques. Le suivi de la faune des arthropodes au cours de 64 nuits de capture a permis la collecte de 2 321 culicoïdes (Ceratopogonidae), 570 moustiques (Culicidae) et 475 phlébotomes (Psychodidae). Quatorze espèces de Culicoides ont été recensées et les espèces dominantes étaient Culicoides imicola (55,96 %), C. paolae (16,07 %), C. circumscriptus (10,29 %) et C. newsteadi (5,77 %). Trois espèces de moustiques ont été collectées, dont Culex pipiens s.l. (96,84 %), Culiseta longiareolata (2,80 %) et Cx. perexiguus (0,36 %). Dix espèces de phlébotomes ont été collectées, dont sept espèces de Phlebotomus (62,70 %) et trois espèces de Sergentomyia (37,30 %); Sergentomyia minuta était l'espèce dominante (34,31 %), suivie de Phlebotomus sergenti (32,42 %), Ph. perniciosus typique (8,63 %), Ph. alexandri (6,94 %) et Ph. riouxi (6,52 %). La coexistence de plusieurs vecteurs dans ces zones d'étude indique la circulation potentielle d'un large éventail d'agents pathogènes, y compris zoonotiques, ce qui nécessite la mise en œuvre de programmes de surveillance et de contrôle pour prévenir l'émergence et la propagation d'épidémies.


Assuntos
Ceratopogonidae , Culicidae , Insetos Vetores , Psychodidae , Animais , Marrocos/epidemiologia , Cães , Psychodidae/classificação , Psychodidae/parasitologia , Insetos Vetores/parasitologia , Insetos Vetores/classificação , Culicidae/classificação , Ceratopogonidae/classificação , Biodiversidade , Feminino , Masculino , Doenças do Cão/parasitologia , Doenças do Cão/epidemiologia , Doenças do Cão/transmissão , População Rural , Abrigo para Animais , Humanos , Zoonoses/transmissão , Zoonoses/parasitologia , População Suburbana
20.
Front Plant Sci ; 15: 1451065, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39345975

RESUMO

Salix psammophila sand barriers degrade under sunlight exposure, resulting in diminished protective performance and shortened service life in desertification control. To address the unresolved issue of photoinduced damage and degradation in sand barriers, we conducted simulations to assess the accelerated damage effect of ultraviolet (UV) rays during solar exposure of S. psammophila sand barriers. Our analysis focused on elucidating the mechanism of UV irradiation in sand barriers by examining the structural and material property changes that occur during the degradation process. The results indicated the following: (1) The discoloration of sand barriers resulting from UV irradiation was primarily ascribed to the modification in lignin content. (2) The morphology and protective performance of S. psammophila sand barriers underwent significant changes following exposure to UV irradiation. The 96-day and 144-day time points of UV exposure are crucial for evaluating the extent of UV degradation in sand barriers. After 192 days of UV irradiation, there was a decrease in mass loss percentage by 3.62%, modulus of elasticity by 8.63%, and modulus of rupture by 6.74%. (3) The lignin, hemicellulose, and cellulose content decreased by 23.12%, 14.30%, and 6.96%, respectively. The impact of UV irradiation on the polysaccharide (cellulose and hemicellulose) in S. psammophila sand barriers was relatively minimal. (4) The carbon binding form in S. psammophila sand barriers underwent a transformation, characterized by a significant decrease in C1 content and an increase in C2 and C3 content. This resulted in a gradual enhancement of the oxidation state and binding energy of carbon. Therefore, to prolong the utilization lifespan of S. psammophila sand barriers, it is essential to address the UV irradiation behavior from the perspective of inhibiting lignin reactions.

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