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Objective To investigate the effect of baicalin on CT26.WT cells of colon cancer in mice, and to discuss the cell death form. Methods CT26.WT cells were divided into four groups including of control group , routine cultured in fresh medium, the baicalin group, added with concentration of 100 μmol·L-1 baicalin, the z-VAD-fmk group, was added with final concentration of 20 μmol·L-1 z-VAD-fmk, and the combination group, added final concentration of 20 μmol·L-1 z-VADfmk,1 h before adding 100 μmol·L-1 baicalin. Then the inhibitory effect of baicalin on cell proliferation and cell viability were detected by CCK-8 method. The changes of nucleus were detected by DAPI staining, the ultrastructure of cells was observed by TEM, and the effect of baicalin on the expression of RIP3 gene and protein in cells was detected by QPCR method and Western blotting. Results Compared with control group, the differences of baicalin group and combination group had statistically significance (P<0.05) . cell death rate for control group was (10.54±0.19) ％ ,for baicalin group was (34.93±0.16) ％ ,for z- VAD group was (11.23±0.59) ％, and combination group was (23.27±1.20) ％ (P<0.01) . Compared with the normal control group, baicalin group showed nuclear concentration and fragmentation. there was obvious nuclear fragmentation in the combination group against baicalin group. The results of electron microscopy showed that the cells of baicalin were necrotic, cell swelling, mitochondria swelling and contents leaking. Baicalin group significantly up - regulated RIP3 mRNA expression (P < 0. 01) and enhanced RIP3 protein expression (P < 0. 05) . Conclusion Baicalin induces the necrosis of ct26. WT cells, and can significantly increase the gene and protein expression of RIP3.
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Objective: To establish an HPLC determination method for the quantitative indicators in Qubai granules( stilbene gly-cosides, ferulic acid and paeonol). Methods: A dual-wavelength HPLC assay was used with the following conditions: a chromatogra-phy column ODS (150 mm×4. 6 mm, 5 μm) was used, the mobile phase was methanol: 0. 1% phosphoric acid with gradient elution, the column temperature was 35℃, and the injection volume was 10 μl. Results: The linear ranges of the three active constituents were 1.56-156.00 μg·ml-1(r stilbeneglycoside=0.999 8)、1.00~100.00 μg·ml-1(rferulicacid=0.999 8),1.61~161.00 μg·ml-1(rpaeonol=0. 999 7), respectively, and the average recoveries of the three constituents were between 100. 33% and 100. 76% with the RSDS less than 2% (n=6). Conclusion: The method is simple, stable and reliable, which can be used for the quality control of Qubai granules.
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Objective:To establish an HPLC method for the determination of entrapment efficiency of matrine microspheres .Meth-ods:A Fortis XiC18 (250 mm ×4.6 mm, 5μm) column was used , the mobile phase consisted of methanol-acetonitrile-3%phosphoric acid solution (11∶80∶9).The flow rate was 0.6 ml· min-1.The detection wavelength was 210 nm, the column temperature was at 25℃and the sample size was 20 μl.Results: The linear range of matrine was 3-150 μg · ml-1 ( r =0.999 7).The recovery was 99.92%(RSD=0.97%, n=9).The entrapment efficiency of matrine microspheres was 81.85%±3.22%(n=3).Conclusion: The method is suitable for the determination of entrapment efficiency of matrine microspheres .
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Objective:To optimize the water extraction process of traditional Chinese medicine ( TMC) Qubai granule. Methods:The orthogonal test was used to study four influencing factors including water amount, soaking time, extraction time and extraction times with dry extract yielding rate and the content of ferulic acid as the evaluation indices. Results:The optimum extraction process was as follows:A2 B1 C2 D2 , namely adding 10-fold amount of water, without soaking in advance, extracting twice with 2 h for each time. Con-clusion:The process is simple, stable and reproducible, which provides basis for the industrial production.
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Objective:To optimize the extraction technology of volatile oil in myrrh. Methods: Using the extraction quantity of volatile oil as the index, the volatile oil was extracted by an essential oil extractor, and U6 (64 ) uniform design was used to investigate the influencing factors such as the immersion time ( A) , the mesh number ( B) , the extraction time ( C) and the additive water amount ( D) . Results:The optimized extraction technology was as follows: myrrh was crushed to obtain 10 mesh powder, soaked for 10 mi-nutes with 8-fold amount of water, and then extracted 3 hours. Conclusion:The quantity of volatile oil extracted from myrrh is accu-rate, the technology is reasonable, and the research provides scientific basis for the extraction of volatile oil in myrrh.
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Objective:To analyze and evaluate the effect after the PIVAS workflow optimization in our hospital. Methods: One-for-one grouping preparation was carried out by pharmacists and nurses. The checking of medicines, solvents and empty ampoules be-fore and after the preparation was performed by pharmacists, and the preparation of infusions was operated by nurses according to the operation specification. The preparation workload, error number in each link and damage of ampoules and finished goods before the op-timization and in 5 months after the optimization were compared. Results:The working efficiency was obviously improved with less time of about 0. 5 h for the same workload, the error rate was decreased by 40%, and the ampoule damage was reduced by 60% before and during the preparation. Conclusion:The PIVAS workflow optimization improves the working efficiency and management level.
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Objective To compare the effects of continuous renal replacement therapy and hemodialysis in the treatment of acute renal failure.Methods 70 patients with acute renal failure were selected and randomly divided into A group and B group according to the wishes of patients.The patients of A group were given continuous renal replacement therapy,the patients of B group were given hemodialysis.APACHE-II scoring system were used,the clini-cal efficacy,adverse reactions,serum creatinine,urea nitrogen,APACHE-II score,the duration of urine more than 600mL were compared.Results The clinical effective rate of A group was 65.7%,which was significantly higher than 54.3%of B group(χ2 =11.321,P<0.05);The serum creatinine and urea nitrogen of A group were (198.1 ± 20.5)μmol/L,(20.3 ±2.6) mmol/L,respectively,which were lower than (283.3 ±19.3)μmol/L,(28.3 ± 1.5)mmol/L of B group,the duration of urine more than 600mL of A group was (12.1 ±0.7)d,which was lower than (18.3 ±2.1)d of B group,APACHE-II score of A group was (14.1 ±2.1)points,which was lower than (17.5 ±1.9) score of B group,the difference was statistically significant( t=12.023,11.321,10.983,9.985,all P<0.05);The incidence of adverse reactions of A group were 5.7%,which was lower than 17.1% of B group(χ2 =12.174,P<0.05).Conclusion The continuous renal replacement therapy and hemodialysis had advantages in treatment of sever acute renal failure,but continuous renal replacement therapy had better clinical results,and fewer adverse reactions.