ABSTRACT
OBJECTIVE@#To observe the biological behavior of canine bone marrow stromal cells (BMSCs) cultured in vitro with the astragalus polysaccharides-chitosan/polylactic acid (AP-C/PLA) and with the chitosan/polylactic acid (C/PLA) and to find a suitable compound material for periodontal tissue engineering.@*METHODS@#BMSCs (induced 14 days by 50 mg/L vitamine C, 10(-8) mol/L dexamethasone, 10 mmol/L beta-sodium glycerylphosphate) were cultured on AP-C/PLA or C/PLA for 5 days respectively. The BMSCs attachment and the morphology were observed with scanning electronic microscope and the combining rates were counted. Type I collagen synthesis was examined with immunohistochemistry staining and the content of osteocalin was determined with radio-immunological method.@*RESULTS@#Combining rates, type I collagen synthesis, and the content of osteocalin of BMSCs on AP-C/PLA were significantly higher than those on C/PLA.@*CONCLUSION@#AP-C/PLA may promote the BMSC proliferation, differentiation and extracellular matrix synthesis, and it can be used as a good scaffold material for bone tissue engineering.
Subject(s)
Animals , Dogs , Female , Male , Astragalus propinquus , Bone Marrow Cells , Cell Biology , Cell Proliferation , Cells, Cultured , Chitosan , Pharmacology , Collagen Type I , Drugs, Chinese Herbal , Pharmacology , Extracellular Matrix , Metabolism , Lactic Acid , Pharmacology , Osteocalcin , Polyesters , Polymers , Pharmacology , Polysaccharides , Pharmacology , Stromal Cells , Cell Biology , Tissue EngineeringABSTRACT
<p><b>OBJECTIVE</b>To investigate the feasibility and clinical results of applying poly-DL-lactic acid (PDLLA) biomembranes in cleft palate repair.</p><p><b>METHODS</b>68 cleft palate patients were divided into study group and control group. The traditional surgical method was used to control group to close the soft cleft palate, and the PDLLA biomembrane was used to study group and implanted into the surgical gap between the periosteum and bone at the hard palate, and fixed with suture. The duration, blood loss at operation, post-operative complication, wound healing and recovery were recorded and compared to conventional cleft palate repair.</p><p><b>RESULTS</b>Operations were successfully completed on all 34 patients. Wound healing of soft palate and uvula was uneventful with no incidence of fistula or dehiscence. The primary healing on tissue defect of hard palate occurred in 29 patients, secondary healing occurred in 3 patients, permanent fistula between the oral cavity and the nasal cavity occurred in only one patients, and 3 patients left over fistula on alveolar process. Compared to traditional cleft palate repair, blood loss and incidence of fistula on alveolar process were decreased; the average surgical time was 89.25 minutes and was not prolonged; and there was no significant increase in post-operative complication.</p><p><b>CONCLUSION</b>Hard cleft palate repair with PDLLA biomembranes is safe, simple and practical with good clinical results and is beneficial to minimize the bad influences towards the development and growth for maxilla of cleft palate patients.</p>
Subject(s)
Adolescent , Adult , Child , Child, Preschool , Female , Humans , Male , Absorbable Implants , Cleft Palate , General Surgery , Feasibility Studies , Guided Tissue Regeneration , Methods , Lactic Acid , Therapeutic Uses , Maxillofacial Development , Palate, Hard , General Surgery , Polyesters , Polymers , Therapeutic Uses , Plastic Surgery ProceduresABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of the viability and new bone formation of osteoblasts by the super high molecular weight poly D,L-lactic acid (SHMW-PDLLA).</p><p><b>METHODS</b>1. The osteoblasts derived from neonatal rat were grown and maintained at steep of SHMW-PDLLA and normal culture medium. The viability and function of the osteoblasts were measured with MTT array. 2. The plate and screws made of SHMW-PDLLA were implanted and fixed at the artificial fractured mandible of dogs. Specimens were gained at 3 and 6 months and examined with macroscopy and SEM.</p><p><b>RESULTS</b>1. There is no significant difference of OD values between the experimental group and the control group (P > 0.05). The SHMW-PDLLA isn't toxic to osteoblast at 1 week and 2 weeks, and the toxicity is 3% at 3 days. 2. There were a lot of new bone formed between the implanted SHMW-PDLLA plate and bone tissues under SEM.</p><p><b>CONCLUSION</b>SHMW-PDLLA hasn't pathological influence on the viability and new bone formation of osteoblasts and it is feasible in tissue engineering of bone.</p>