ABSTRACT
The present study was carried out to study the effect of antioxidants on oxidised LDL + VLDL and found that vitamin E, eugenol and tincture of crataegus (antioxidants) inhibited oxidation of (LDL + VLDL) similar to standard antioxidant (butylated hydroxy toluene). Vitamin C acted as an antioxidant at lower concentration, and prooxidant at higher concentration.
Subject(s)
Antioxidants/pharmacology , Crataegus , Diabetes Mellitus, Type 2/blood , Eugenol/pharmacology , Humans , Lipoproteins, LDL/blood , Lipoproteins, VLDL/blood , Oxidation-Reduction , Plant Extracts/pharmacology , Thiobarbituric Acid Reactive Substances/metabolismABSTRACT
The effect of eugenol on the antioxidant status of the rat intestine after short and long term (15 days and 90 days respectively) oral administration of 1000 mg/kg.b.wt (a dosage which has been reported to be highly hepatoprotective) was studied. The level of lipid peroxidation products (TBARS) and the activities of glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD) and catalase (CAT) were found to be near normal on eugenol treatment. The level of glutathione (GSH) did not show any change on 15 days of eugenol treatment, but it was increased significantly on 90 day eugenol treatment. The activity of glutathione-S-transferases (GSTs) was increased significantly in both 15 day eugenol treated and 90-day eugenol treated groups. The results suggest that eugenol is nontoxic, protective and induces glutathione-S-transferases (GSTs) and thereby it may facilitate the removal of toxic substances from the intestine.
Subject(s)
Animals , Antioxidants/pharmacology , Catalase/metabolism , Eugenol/pharmacology , Glutathione/metabolism , Glutathione Transferase/metabolism , Intestines/drug effects , Male , Rats , Rats, Wistar , Superoxide Dismutase/metabolism , Thiobarbituric Acid Reactive Substances/metabolismABSTRACT
MeAN administration (40mg/kg body wt/day (i.e. 1/5 of LD50) resulted in increased levels of lipid peroxidation products, conjugated dienes and lipofuscin-like substances in rat liver. Significant decrease in GSH and a decreased activity of hepatic SOD, CAT and GPx were observed. There was also an increase in glutathione S-transferase and G6PD activities, decreased plasma ceruloplasmin and vitamin C implying oxidative stress caused by MeAN.
Subject(s)
Animals , Antioxidants/metabolism , Glutathione/metabolism , Lipid Peroxidation/drug effects , Lipofuscin/metabolism , Liver/drug effects , Male , Methacrylates/toxicity , Nitriles/toxicity , Oxidative Stress/drug effects , Rats , Rats, WistarABSTRACT
A crude extract containing some toxic furanoterpenoids was isolated from F. solani infected sweet potatoes. Chronic administration of the crude extract to male albino rats at a dosage of 1 mg/kg body weight/day for 21 days brought about a sharp increase in the thiobarbituric acid reactive substances and a depression of glutathione levels in the lung and liver homogenates. The antioxidant defense system was affected as evident from a significant fall in the activities of the enzymes, superoxide dismutase, catalase, glutathione peroxidase, glucose-6-phosphate dehydrogenase and glutathione-S-transferase. Such an alteration could be the reason for the lung and liver damage caused by these toxic furanoterpenoids.
Subject(s)
Animals , Furans/isolation & purification , Fusarium/metabolism , Liver/drug effects , Lung/drug effects , Male , Mycoses/metabolism , Oxidative Stress/drug effects , Rats , Rats, Wistar , Terpenes/isolation & purification , Vegetables/metabolismABSTRACT
Tincture of Crataegus (TCR), an alcoholic extract of the berries of Crataegus oxyacantha, when administered to rats fed a hyperlipidemic diet (HLD), could prevent the elevation in plasma lipid levels. A significant decrease in lipid deposits in liver and aorta was also observed. Analysis of the plasma lipoprotein profile showed that TCR produced remarkable reduction in the increased levels of cholesterol, triglycerides and phospholipids in the low density lipoprotein (LDL) and very low density lipoprotein (VLDL) fractions in hyperlipidemic rats. Histological examination showed severe fatty vacuolation and degeneration of liver of HLD fed rats. TCR administration had an ameliorating effect on these changes. Agarose gel electrophoretic pattern of plasma lipoproteins also indicated that the drug brought down the raised levels of the atherogenic beta-lipoproteins in hyperlipidemic rats.
Subject(s)
Animals , Hypolipidemic Agents/pharmacology , Cholesterol/blood , Diet, Atherogenic , Hyperlipidemias/blood , Lipids/blood , Lipoproteins, LDL/blood , Lipoproteins, VLDL/blood , Male , Phospholipids/blood , Plant Extracts/pharmacology , Plants, Medicinal , Rats , Rats, Wistar , Triglycerides/bloodABSTRACT
Intraperitoneal administration (1 mg/kg body/wt./day for 21 days) of crude extract of furanoterpenoids, isolated from F. solani damaged I. batatas caused pulmonary oedema in albino rats. The elevated broncho alveolar lavage (BAL) angiotensin converting enzyme (ACE), lactate dehydrogenase (LDH) and protein levels indicated lung damage. The estimation of pulmonary extracellular surfactant phospholipids showed an alteration in various phospholipid fractions.
Subject(s)
Animals , Fusarium/physiology , Lung/drug effects , Male , Plant Extracts/toxicity , Rats , Rats, Wistar , Terpenes/toxicity , Vegetables/microbiologyABSTRACT
Administration of amiodarone (AD) to rats leads to marked damage to liver, as evidenced by pathological changes and significant increases in activities of serum marker enzymes and levels of lipids like cholesterol and phospholipids with no alteration in the triglyceride levels. The risk factor, that is the total cholesterol/HDL cholesterol ratio, exhibited increase in the experimental animals, indicating that amiodarone treatment may lead to the development of coronary heart disease.