Update status of toxoplasmosis and brucellosis in small ruminants and human in Sharkia province

Toxoplasmosis and brucellosis are widely distributed zoonotic diseases affecting a wide spectrum of animals and humans Serum samples were collected from 182 sheep, 294 goats and 60 human from different localities of Sharkia province [Zagazig - Abo-Hammad - Fakous and Abo-Kabeer] in the period between March - July 2005 and serologically tested for presence of specific antibodies against toxoplasmosis and brucella species Out of 182 sheep, 48 [26 4%] were sero-positive for toxoplasmosis By using of enzyme linked immunosorbent assay [ELISA], 17 [9.3%] were IgG positive and 6 [3.3%] were 1gM positive Among goats 38 [12.93%] were positive by IHAT and by ELISA 12 [4.08%] were positive for presence of IgG as well as 9 [3.06%] were positive for presence of IgM Rose Bengal Plate Antigen [RBPA], Buffered Acidified Plate Antigen [BAPA] Rivanol test [Riv T] and serum agglutiation Test [SAT] were applied to identify brucellosis. Out of 182 sheep sera samples, 21[11.54%] were sero-positive; Meanwhile out of 294 goat sera, 12 [4.08%] showed sero-positivity to brucellosis. Brucella melitensis biovar - 3 was isolated from milk samples and tissue specimens [especially lymph nodes] of the reactor animals and from 17 ewes sero-positive to brucellosis there were 8 positive for isolation and identification of Brucella melitensis, whereas there were 3/15 sero-positive goats [20%] so that control measures of toxoplasmosis and brucellosis were suggested. Concerning to human the incidence of toxoplasmosis and brucellosis were 28.3% and 8.3% respectively

Protective role of diethyldithiocarbamate against toxicity of some new antibacterial and antitumor platinum[II] and [IV] organochelates

Aim: Five newly synthesized Schiff base complexes of the platinum [II] and [IV] were screened for their in vitro antibacterial and antitumor activity. Meanwhile, the protective role displayable by diethyldithiocarbamate [DDTC] against the inducible toxicity of two of platinum chelates was investigated as well. Their minimal inhibitory concentrations [MICs] were determined against narrow range of Gram negative and Gram positive bacteria while the antitumor potentials were tested against Ehrlich ascites carcinoma [EAC]. Half LD50 of the two selected antitumor complexes [naphthylaldehyde and diamino hexane [Pt [II] NDAH] and Pt [IV] NEDA] was i.p. injected in male Sprague Dawley rats then their biochemical consequences were investigated. The mode of action studies on intact cells of the most sensitive bacterium [Bacillus subtilis NRRL-B-4378] revealed that Schiff base Pt[IV] chelate derived from naphthylaldehyde and ethylene diamine [Pt [IV] NEDA] had reasonable reducing effects on microbial RNA and protein contents, while DNA was powerfully affected. Regarding their toxicity, Pt[IV] NEDA was found to be less hepato and nephro toxic than Pt[Il] NDAH. The hepatotoxic and nephrotoxic effects of these two platinum complexes seem to be reasonably ameliorated in animals post treated with DDTC as indicated by nearly restoration of normal values of the studied biochemical parameters

Vascular and hormonal changes before and after laparoscopic ovarian electrocautery in patients with polycystic ovarian disease

Forty infertile patients with PCOD were enrolled in the study, they were undergone laparoscopic ovarian electrocautery. Before the operation, all cases were subjected to a good history with a thorough clinical examination together with transvaginal ultrasonic examination, hormonal profile [FSH, LH, prolactin and DHEA] in the early follicular phase and color Doppler of uterine and ovarian arteries with determination of pulsatility index [PI] were done to all patients. After operation hormonal profile was assessed one day, one month and three months after electrocautery, Doppler study of ovarian and uterine arteries, folliculometry for three consecutive cycles. As regard Doppler before drilling, flow velocity patterns of uterine and ovarian vessels showed high pulsatility index [PI], which showed gradual decrease along the three cycles after drilling. As regard, hormonal profile, FSH showed significant rise, while LH and LH/FSH ratio showed significant fall along the three cycles, DHEA showed no significant change one day after drilling, but significantly decreased one month and three months after drilling. On the other hand, prolactin showed no significant change

Perioperative evaluation of patients with liver cirrhosis

This work studied 30 patients of both sexes aged between 30-73 years, weighing between 66-95 kg undergoing upper abdominal surgical procedures under general anesthesia. The patients were randomly assigned into three categories according to Child-Pugh and Moemen classification. PT prolonged, serum creatinine and albumin level were measured compared before and after preparation. Intraoperative hemodynamics, morbidity and mortality were evaluated. In this study, it was observed that the PT prolonged and serum creatinine were decreased and albumin level was increased after preparation as compared to baseline value. Although hemodynamic changes during operation were sometimes statistically significant, this was of no clinical importance. Comparison of Child-Pugh and Moemen scores and classifications by statistical study showed a significant positive correlation between them. In the present study, the morbidity was reported by 30% in all patients within 30 days after operation. The overall mortality rate was 6.6% within 30 days after operation