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Objective To investigate the expression of p53 in breast cancer and its clinical significance .Methods The clinical pathological data of 214 cases of breast cancer patients were collected ,and retrospective analyzed of p53 protein expression and its correlation with clinical pathological features .Results The positive rate of p53 was 52 .3% .The expression p53 was no correlation with age ,the size of the lesion(P> 0 .05) ,it was correlation with lymph node metastasis ,histological classification and molecular subtype(r=0 .396 ,0 .309 ,0 .167 ,P=0 .000 ,0 .000 ,0 .014) ,and also have negative correlation with the expression rate of ER ,PR (r= -0 .561 ,-0 .315 ,P=0 .000 ,0 .000) ,positive correlation with HER2 and Ki67(r=0 .374 ,0 .153 ,P=0 .000 ,0 .026) .In the group of triple negative breast cancer (TNBC) and the group of non-TNBC ,the difference was statistically significant of expression of p53 (P<0 .05) .Conclusion p53 protein has closely related with the development ,relapse and metastases of breast cancer ,the detection of p53 can be used to guide the individualized therapy and as a reference index to evaluate the prognosis of breast cancer .
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Objective To evaluate the roles of signal transducer and activator of transcription 3(STAT3) played in delayed xenograft rejection.Method Mice-to-rats cardiac xenograft model was established and recipients were administrated by inhibitor of phosphorylation of STAT3,5,15-Diphenylporphyrin(DPP),and immunosuppressive agent,ciclosporin A (CsA) alone or both.The survival of graft was analyzed.Immunohistochemistry and immunoblotting were utilized to detect the phosphorylated STAT3 (p-STAT3),and real-time polymerase chain reaction was used to assess STAT3-targeted genes in grafts.Result The expression of p-STAT3 was increased significantly with the prolonged survival in grafts (P ＜ 0.05).Administration of DPP and CsA both significantly prolonged survival of the grafts (P＜0.05),and decreased the expressions of STAT3-targeted genes including Bcl-xl,Bcl-2,Cyclin D1,C-myc and VEGF (P＜0.05).DPP and CsA exerted the synergistic effects.Conclusion STAT3,which is persistently activated in cardiac xenografts,probably up-regulates downstream genes to promote proliferation and to inhibit apoptosis of endothelial cells,leading to the activation of endothelial cells and delayed xenograft rejection.
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glucagonoma is a rare islet alpha-cell tumor. We report a case of glucagonoma in a 55-year-old male patient with such clinical findings of necrolytic migratory erythema, diabetes mellitus, body weight loss, and anemia. CT examination found a space-occupying lesion in the pancreas, and an elevated serum glucagon level indicate the diagnosis of glucagonoma, which was confirmed postoperatively by pathological examination of the tumor tissue. A definite diagnosis of glucagonoma relies on pathological report, and so far no standard treatment strategy has been available for this tumor. Surgical resection is an effective means for treatment of glucagonoma.
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Humans , Male , Middle Aged , Glucagonoma , Diagnosis , Pathology , General Surgery , Pancreatectomy , Pancreatic Neoplasms , Diagnosis , Pathology , General SurgeryABSTRACT
Objective To discuss the value of mammotome biopsy of breast cakified lesions under the real time guidance of mammography.Methods The data of 42 patients with 44 mammary gland calcification(2 patients with bilateral lesions)who underwent mammotome biopsy under the real time guidance of a mammography from Jan.2011 to Feb.2012 in the First Affiliated Hospital of Chongqing Medical University were retrospective analyzed.Rapid paraffin pathology was conducted.Results Among the 44 lesions,34 were benign(27 hyperplasia,2 fibroadenoma,1 lipoma,1 cyst,3 others)and 10 were breast cancer(a benign lesion diagnosed as breast cancer by postoperative biopsy was included).Among the 42 patients,32 patients had benign lesions,accounting for 76.2％ (32/42),10 patients had breast cancer,accounting for 23.8％ (10/42).All lesions were completely resected during the operation.Malignant lesions were given treatment such as modified radical mastectomy,breast conservation surgergery,chemotherapy and so on.A single lesion resection took about 20 minutes.During the follow-up of 3 months,no significant event happened.Conclusions Through the qualitative diagnosis of mammotome biopsy under the real time guidance of mammography,we can get early diagnosis of breast cancer.Early diagnosis and early treatment can improve the life quality and survival time.It is more important to detect early breast cancer which was considered benign lesions clinically.Mammotome biopsy under the real time guidance of mammography has several advantages including easy to operate,accurate positioning and less complications,making it the preferred approach in managing mammary gland calcification especially suspicious malignant calcification.
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Objective Comparing the influence of neoadjuvant chemotherapy on sentinel lymph node biopsy and axillary lymph nodal staging. Methods From June 2005 to June 2007 one hundred and sixty five breast cancer patients of stage Ⅰ,Ⅱa,Ⅱb and Ⅲ underwent sentinel lymph node biopsy(SLNB) using 99mTc combining methylene blue dye as tracer to help axillary lymph node dissection(AND) were retrospectively reviewed. Sixty five patients staged Ⅱb or Ⅲ were treated with three circles neoadjuvant chemotherapy prior to SLNB and AND , another one hundred patients staged ⅠorⅡa had SLNB and AND directly without neoadjuvant chemotherapy.Results Average 14.60 axillary lymph nodes were retrieved in patients had neoadjuvant chemotherapy and 14.74 lymph nodes in patients did not have neoadjuvant chemotherapy(P>0.05),a mean number of 1.46 sentinel lymph nodes in neoadjuvant chemotherapy group and 1.5 sentinel lymph nodes in non-neoadjuvant chemotherapy(P>0.05), sentinel lymph node identification rate 96.9% in neoadjuvant chemotherapy group and 97% in non-neoadjuvant chemotherapy(P>0.05), the false negative rate was 4.6% in neoadjuvant chemotherapy group and 4% in non-neoadjuvant chemotherapy group(P>0.05). Conclusions The mean number of lymph nodes,sentinel lymph nodes,SLN identification rate and false-negative rates after neoadjuvant chemotherapy are similar to those seen in patients without neoadjuvant chemotherapy.The SLNB can accurately predict lymph node status of axillary lymph node following neoadjuvant chemotherapy.
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G protein-coupled receptor 30(GPR30) was a novel estrogen receptor identified as membrane associated receptor in the late 1990s.This new member of estrogen receptors was independent of the classic nuclear estrogen receptor ? and ? due to the low homology and signifi cant difference between them.It was reported that GPR30 localized endoplasmic reticulum predominantly,which was expressed in diverse cancer cells and a wide range of systems throughout the body.The rapid non-genetic response,partially at least,transcription regulation of estrogenic effects were mediated by the novel receptor via transactivation of epidermal growth factor receptor and modulation of second messengers such as cyclic adenosine monophosphate(cAMP) and Ca2+.These pathways,possibly coordinate with ER?,were involved in various physiological,physiopathological and carcinogenesis process.Theoretically,GPR30 would be a novel therapeutic target in estrogen-related diseases such as breast carcinoma.
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Medical aesthetics education is a part of quality education of medical student.Breast carcinoma conserved treatment is the concrete application of medical aesthetics education in clinic practice.The courses should be arranged according to the clinical practice to attain the goal of improving the quality of medical students in an all-round way we.
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Objective By reproducing mouse to rat heart xenograft,to determine the effect of leflunomide on NF-?B P65 in the transplanted heart.Methods The recipient rats were randomly divided into 4 groups:group A(n=6)receiving no immunosuppressant as control;group B(n=6)cyclosporine(CsA);group C(n=6)leflunomide(Lef),group D(n=6)Lef plus CsA.The expressions of NF-?B P65 protein and NF-?B P65 DNA binding activity in transplanted heart tissues were determined by immunohistochemistry,Western blot and electrophoretic mobility shift assay(EMSA).Results Administration of CsA alone had no appreciable effect on the survival time of xenografts [(2.50?1.05)d] and could not suppress the protein expression of NF-?B P65 [(263.09?28.81),IOD] and NF-?B P65 DNA binding activity [(227.49?14.83),IOD] in the tissues of xenografts.Administration of Lef alone could significantly prolong the survival time of xenografts [(4.17?1.33)d] and could suppress the protein expression of NF-?B P65 [(173.48?5.85),IOD] and NF-?B P65 DNA binding activity [(109.96?12.46),IOD](P
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Objectiver To establish murine breast precancerous model and evaluate the effect of exogenous estrogen and tamoxifen. Method 50 female Wistar rats were divided into contol group (n＝12), the other 38 received low dose carcinogen (DMBA) by gastrogavage, and then divided into DMBA group (common diet,n＝8); DMBA+DES group(DES added chow,n＝15); and DMBA+TAM group (TAM added chow,n＝15). 3 months later, the status of breast hyperplasia, AgNOR and DNA content were measured. Results All indexs in control group and DMBA+TAM group were normal.However,in DMBA group and DMBA+DES group,the breast hyperplasia rate was 75%, 93.3%, AgNOR was 3.71±0.76, 5.60±0.42, DNA was 88.59±7.17, 137.75±3.13 respectively.Compared with control group and DMBA+TAM group, the differences of indexes in DMBA and in DMBA+DES group were significant (P<0.01). Conclusion Low dosage DMBA administered by gastrogavage established breast precancerous model. Exogenous estrogen promotes the development of breast precancerous disease induced by DMBA, while TAM suppresses the canceration.
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Objective To investigate the diagnosis and management of pancreatic injury. Methods The clinical data of 23 patients suffering from pancreatic injury treated by surgery in recent 15 years were analyzed retrospectively. According to the pancreas organ injury scale of the American Association for the Surgery of Trauma, there were 8 cases in grade I, 8 in grade II, 5 in grade III, 1 in grade IV and 1 in grade V. Results Only 2 patients were diagnosed preoperatively out of the 19 patients.About 63.2%(12/19)of the cases had elevated levels of serum amylase. All the patients were cured.In this series,8 cases occurred external pancreatic fistula, 4 pseudocysts, 2 intra-abdominal infections and 1 hyperglycemia postoperatively. Internal pancreatic fistulas were found in all the non operation patients. Conclusions The serum amylase determinations is neither sensitive nor specific for diagnosis of pancreatic injury. Early diagnosis and correct operative pracedure are important to decrease the postoperative complications and improve the outcome.
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Objective To study the expression and clinical significance of EphB4 in breast infiltrating ductal carcinoma.MethodsSP immunohistochemical technique was used to examine the expression of EphB4 in 65 cases of breast infiltrating ductal cancer and 12 matched adjacent tissue samples.ResultsThe level of EphB4 in breast infiltrating ductal cancer was significantly higher than that of adjacent normal tissues(P0.05).ConclusionEphB4 plays a crucial role in the occurrence and development of breast cancer.
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Objective:Using modified cuff technique discribed by Heron to establish mouse-to-rat cardiac xenotransplantic model.Methods:NIH mice and Wistar rats were served as donors and recipients respectively.Hearts from the donors were transplanted heterotopically into the recipients by cuff technique.The aorta of the donor heart was anastomosed end-to-end to the right common carotid artery of the recipient and the pulmonary artery was anastomosed end-to end to the right external jugular vein using tube of polythene.Results:30 times of transplantation were undertaken with the successful rate of 93.3%.The mean survival time of the xenografts was 2.13?0.8 days.Conclusion:These data suggest that this method with its simplicity,rapidity and reliability is a good model for studying xenotransplantation.
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Objective To reproduce mouse to rat heart transplantation model and determine the expression and DNA binding activity of NF-?B P65 in xenograft heart.Methods Six NIH mice were used as heart donors and six Wistar rats as heart recipients.After the transplantation,the transplanted hearts without any drug intervention were prepared for experiment after cardiac arrest.The hearts from another six NIH mice served as normal controls.The expression of NF-?B P65 protein and NF-?B P65 DNA binding activity in xenograft heart were determined by Western blotting and electrophoretic mobility shift assay(EMSA).Results The expression level of NF-?B P65 and NF-?B P65 DNA binding activity in transplanted hearts were higher than control hearts(P