Lipidomic investigation of the protective effects of Polygonum perfoliatum against chemical liver injury in mice / 中西医结合学报

OBJECTIVE@#Recent investigations have demonstrated that Polygonum perfoliatum L. can protect against chemical liver injury, but the mechanism behind its efficacy is still unclear. Therefore, we studied the pharmacological mechanism at work in P. perfoliatum protection against chemical liver injury.@*METHODS@#To evaluate the activity of P. perfoliatum against chemical liver injury, levels of alanine transaminase, lactic dehydrogenase, aspartate transaminase, superoxide dismutase, glutathione peroxidase and malondialdehyde were measured, alongside histological assessments of the liver, heart and kidney tissue. A nontargeted lipidomics strategy based on ultra-performance liquid chromatography quadrupole-orbitrap high-resolution mass spectrometry method was used to obtain the lipid profiles of mice with chemical liver injury and following treatment with P. perfoliatum; these profiles were used to understand the possible mechanisms behind P. perfoliatum's protective activity.@*RESULTS@#Lipidomic studies indicated that P. perfoliatum protected against chemical liver injury, and the results were consistent between histological and physiological analyses. By comparing the profiles of liver lipids in model and control mice, we found that the levels of 89 lipids were significantly changed. In animals receiving P. perfoliatum treatment, the levels of 8 lipids were significantly improved, relative to the model animals. The results showed that P. perfoliatum extract could effectively reverse the chemical liver injury and significantly improve the abnormal liver lipid metabolism of mice with chemical liver injury, especially glycerophospholipid metabolism.@*CONCLUSION@#Regulation of enzyme activity related to the glycerophospholipid metabolism pathway may be involved in the mechanism of P. perfoliatum's protection against liver injury. Please cite this article as: Peng L, Chen HG, Zhou X. Lipidomic investigation of the protective effects of Polygonum perfoliatum against chemical liver injury in mice. J Integr Med. 2023; 21(3): 289-301.

Effects of different extraction methods on chemical compositions and biological activities of polysaccharides from Lycium barbarum / 中国中药杂志

In this study, five polysaccharides from Lycium barbarum(LBPs)(LBP-1-LBP-5) were selectively extracted by different extraction methods, and the chemical composition, structural characteristics, and biological activities of LBPs were explored. The results of chemical composition analysis showed that alkaloids were not detected in the five LBPs. The total polysaccharide content was(81.95%±1.6%)-(92.96%±0.76%), the uronic acid content was(8.26%±0.46%)-(24.81%±0.46%), and the protein content was(0.06%±0.03%)-(1.35%±0.13%). The monosaccharide compositions of the five LBPs were basically same, mainly including glucose, xylose, and galactose. However, there was significant difference in the content ratio of different monosaccharide. The results of infrared spectra analysis indicated that the five LBPs had typical infrared spectral characteristics of polysaccharides. The results of nuclear magnetic resonance characteristic spectrum analysis revealed that the five LBPs had two configurations of α and β. Meanwhile, there were triple helix structures in LBP-2, LBP-3, and LBP-4, which enhanced the activities of polysaccharides. The results of activities screening suggested that the biological activities of the five LBPs were significantly different. LBP-3 showed the highest lipid oxidation clearance rate, and its antioxidant activity was equivalent to that of the positive control group. The inhibitory rate of LBP-4 on α-amylase and its activation rate of alcohol dehydrogenase were better than those of other fractions, and the inhibitory rate of LBP-4 on α-amylase was slightly higher than that of the positive control group when the mass concentration was 10 g·L~(-1). LBP-2 showed stronger inhibitory activity against α-glucosidase and hyaluronidase. This study provides references for the precise development and utilization of LBPs.

Research progress on intestinal microecology regulating mechanism and biological activities of polysaccharides / 中国中药杂志

Intestinal microecology is an important defense system in the human body. The intestinal flora is the core micro-ecosystem in the human intestine. It has a symbiotic relationship with the overall functions of the body. It has strong metabolic activity to maintain the normal functioning of the body and resist the invasion of various viral antigens in the body. Playing a protective function,the imbalanced intestinal microecology can cause various diseases. Polysaccharides can be extracted from a wide range of sources and have low toxicity and side effects. They have attracted wide attention because of their anti-tumor, anti-oxidant, anti-inflammatory and other biological activities. Studies have demonstrated that polysaccharides can regulate intestinal microecological disorders. According to the studies in recent years, this review summarizes that polysaccharides mainly modulate intestinal microecological disorders through regulating the composition of intestinal flora, improving the metabolism of the flora, and repairing the intestinal tract barrier. On the basis of these mechanisms of action, this paper elaborates the anti-tumor, immunomodulatory, and anti-inflammatory activities of polysaccharides. This paper can provide reference for the future research on the intestinal microecology-regulating mechanism and biological activities of polysaccharides.

Nomograma prediction of the surgical treatment in triad of elbow / 中国骨伤

OBJECTIVE@#To establish an individualized Nomogram prediction model for predicting the postoperative recovery of patients with triad of elbow (TE) by analyzing risk factors of triad of elbow joint.@*METHODS@#From January 2012 to December 2018, 116 patients with TE who met the criteria were collected. The independent risk factors were screened by univariate Logistic regression analysis. The statistically significant risk factors were included in the multivariate Logistic regression model. The R software was used to establish the Nomogram diagram model to predict the postoperative recovery of TE patients. C index was used to verify the discrimination, Calibration plot of the model, and the decision curve (decision curve analysis, DCA) to verify the net clinical benefit rate of the model.@*RESULTS@#Forty-four of the 116 patients with TE developed symptoms after operation, with an incidence of 37.93%. Age (@*CONCLUSION@#The Nomogram for predicting postoperative results of TE patients based on six independent risk factors:age, work, smoking, Mason classification of radial head, Regan-Morrey classification of coronal process and immobilization time of elbow joint after operation, has good distinguishing capacity and consistency. Thepredictive model could help clinicians to identify high risk population and establish appropriate intervention strategies.

Mass spectrometry imaging technology and its application in medicinal plants research / 中国中药杂志

As an important molecular imaging technology, mass spectrometry imaging(MSI) converts the ionic strength, mass-charge ratio and coordinates of ionized molecules in specific areas recorded by mass spectrometer into a pixel model by special imaging analysis software, and reconstructs the spatial ion distribution image of the compounds tested. It has the advantages of simple sample preparation, high sensitivity and no need for labeling. In recent years, MSI technology has been widely used in medicine, pharmacy, botany and other fields. Among them, the application of MSI technology in the research of medicinal plants provides a new technical idea for clarifying the pharmacological mechanism, improving the curative effect, tracking the distribution of toxic components, and makes an important contribution to the further development and utilization of medicinal plants. This review summarizes the research results of MSI technology in recent years for identification of medicinal plants, distribution of metabolites, pathway of active ingredient synthesis, medicinal safety and plant defense, and discusses the advantages and disadvantages of different research methods. Finally, the advantages and practical obstacles of MSI technology in medicinal plant research are briefly pointed out, and the prospects for the future development of MSI technology and medicinal plant research are also prospected.

Research progress on anti-hepatocellular carcinoma mechanism of active ingredients of traditional Chinese medicine / 中国中药杂志

According to the latest data, the annual mortality rate of liver cancer in China ranks the second among malignant tumors, and it has become one of the most fatal cancers in urban and rural areas. This article starts with the active ingredients of traditional Chinese medicines for liver cancer to summarize the relevant literature at home and abroad, with the hope to provide theoretical references for the development of traditional Chinese medicines against liver cancer. The results show that the active ingredients of traditional Chinese medicines such as Chinese herbal extracts, flavonoids, polysaccharides, alkaloids, saponins, volatile oils, terpenes, quinones, phenols, bioenzymes, and protein components are the entry points, mainly by inhibiting the proliferation of cancer cells, blocking the cancer cell cycle, inhibiting the invasion and metastasis of cancer cells, regulating signal pathways, regulating vascular endothelial growth factor(VEGF) and reactive oxygen species(ROS), etc. to play the effects against liver cancer. It can be seen that the active ingredients of traditional Chinese medicine with coordinated intervention effects at multiple levels and multiple targets are expected to become ideal medicines for the treatment of liver cancer.

Research progress on mechanism of active ingredients in traditional Chinese medicine for gastric cancer / 中国中药杂志

Gastric cancer is a disease with high mortality, which threatens the health of people for a long time. At present, the main treatment methods are surgery and chemotherapy, but these methods have great harm to the human body. However, it is found that the active ingredients of traditional Chinese medicine have an obvious therapeutic effect in the adjuvant treatment of the tumor. Therefore, the active ingredients of traditional Chinese medicine have become a research hotspot in the anti-tumor field. In recent years, many related researchers have been particularly active in studying the in vitro activity and mechanism of active ingredients of traditional Chinese medicine on human gastric cancer cells. In this paper, the Chinese herbal medicine extracts, polysaccharides, alkaloids, saponins, flavones, terpenes, quinones, volatile oils, esters, phenols, protein components and other active ingredients of Chinese medicine were used as the starting points to investigate the anti-gastric cancer mechanism, such as inhibiting cell proliferation and inducing apoptosis of cancer cells, inhibiting cell invasion and migration; inhibiting over-expression of vascular endothelial growth factor(VEGF); interfering with cell mitosis; and regulating cell signaling pathways. Their in vitro inhibitory activity and mechanism for gastric cancer cells were described in this study, providing a theoretical reference for the development and application of anti-gastric cancer drugs.

Exploration of modern scientific connotation of Miao medicine Qizu Xueyu syndrome and intervention mechanism of Kangfuyan Capsules / 中国中药杂志

The aim of this paper was to explore the modern scientific connotation of Qizu Xueyu syndrome in Miao medicine and the intervention mechanism of Kangfuyan Capsules on Qizu Xueyu syndrome. Under the guidance of the theory of Miao medicine,an experimental animal model was constructed to characterize Qizu Xueyu syndrome in Miao medicine,and its modern scientific connotation was explored from the perspectives of general signs,blood lipid index,serum biochemical index and immune index. Furthermore,the relevant experimental animals were given Kangfuyan Capsules for intervention,then observed the corrective effect of Kangfuyan Capsules on abnormal physiological and biochemical indexes of the experimental animal model. The model animals showed signs such as mental depression,rough coat,dull and glossy,and the writhing behavior changed significantly. Low density lipoprotein cholesterol,creatine phosphate kinase,Ig G,urea nitrogen,creatinine,direct bilirubin,urea,urinary biliary and liver index were significantly higher than those in the blank group( P< 0. 01). The blood sugar and thymus index were significantly lower than those in the blank group( P <0. 01). After the intervention of Kangfuyan Capsules,the physiological and mental state of the experimental animals improved,their weight increased and their writhing behavior improved. Creatine phosphate kinase( CK) was significantly higher than that of Qizu Xueyu group( P<0. 01),direct bilirubin was significantly lower than that of Qizu Xueyu group( P<0. 01),immune indexes had no significant difference,urinary bilirubin returned to normal. The modern scientific connotation of Qizu Xueyu syndrome in Miao medicine can be characterized by ischemia,tissue and cell damage,infection and abnormal liver and kidney function caused by smooth muscle spasm. Kangfuyan Capsules can relieve uterine smooth muscle spasm,improve local blood circulation,repair muscle cell injury,protect liver and improve renal function.

New strategy for antagonizing drug resistance: targeting survivin / 中国药理学通报

Multidrug resistance ( MDR) often leads to failure of cancer chemotherapy. survivin, a member of the inhibitor of apoptosis proteins (IAP) family, is overexpressed in almost all kinds of tumor cells. Importantly, survivin has been identified as one of the most important molecules involved in the regulation of MDR. Therefore, inhibiting the activity of survivin or down-reg-ulating the expression of survivin might be an effective strategy to overcome tumor MDR. In this article, the roles of survivin in-volved in the development of drug resistance and the strategies to overcome drug resistance by targeting survivin are thus re-viewed.

Case-control study of pedicle screw fixation combined with vertebroplasty and injured vertebra pedicle fixation in treatment of osteoporotic thoracolumbar burst fractures / 中国骨伤

<p><b>OBJECTIVE</b>To compare the curative effect of short-segment pedicle screw fixation combined with vertebroplasty and injured vertebra pedicle fixation in treating osteoporotic thoracolumbar burst fractures.</p><p><b>METHODS</b>A retrospective study was performed for 52 patients with thoracolumbar burst fractures from August 2010 to August 2015. Among them, 27 patients(group A) were treated with short-segment pedicle screw fixation combined with vertebroplasty, including 17 males and 10 females, aged from 54 to 68 years old with an average of(61.01±5.41) years, 16 cases were type A3 and 11 cases were type A4 according the new AO typing. Other 25 patients (group B) were treated with short-segment pedicle screw fixation combined with injured vertebra pedicle fixation, including 12 males and 13 females, aged from 55 to 66 years old with an average of (59.28±6.12) years, 18 cases were type A3 and 7 cases were type A4 according the new AO typing. Operation time, intraoperative bleeding volume, complication, image data and clinical effect were compared between two groups.</p><p><b>RESULTS</b>All the patients were followed up for 12 to 15 months with an average of (12.4±2.1)months. There was no significant difference in general data(including gender, age, injured site, preoperative VAS score, Cobb angle, injured vertebral anterior border height) between two groups. There was no significant differences in operation time, intraoperative bleeding volume between two groups. Preoperative, one week after operation and final follow-up, VAS scores were 5.2±0.5, 1.2±0.2, 0.8±0.1 respectively in group A and 5.0±0.6, 2.5±0.4, 1.3±0.2 in group B; injured vertebral anterior border height were (49.4±6.8)%, ( 94.5±1.2)%, ( 94.1±3.7)% respectively in group A and (48.2±7.0)%, ( 94.3±4.1)%, ( 90.0±2.3)% in group B;Cobb angles were (20.4±5.2) °, (2.5±1.8) °, (4.4±1.7)° respectively in group A and (19.8±6.8)°, (2.4±1.7)°, (7.0±1.2)° in group B. At final follow-up, VAS, Cobb angle, injured vertebral anterior border height in two groups were obviously improved(<0.05). Postoperative at 1 week and final follow-up, VAS score of group A was lower than that of group B(<0.05);and there was no significant difference in Cobb angle between two groups(>0.05); there was significant difference in injured vertebral anterior border height between two groups(<0.05). The complication of internal fixation failure had 1 case in group A and 4 cases in group B.</p><p><b>CONCLUSIONS</b>For the treatment of single osteoporotic thoracolumbar burst fractures, short-segment pedicle screw fixation combined with vertebroplasty is better than combined with injured vertebra pedicle fixation in clinical effect, it can relieve pain, maintain injured vertebral height and sagittal alinement, reduce the complications associated with internal fixation, and be worth spread in clinic.</p>

Structure-activity relationship of plant polysaccharides / 中国中药杂志

Plant polysaccharides have a variety of biological activities and have become the focus of many fields such as the areas of medicine and food for the features of safety, high efficiency and low toxicity. The chemical structure of plant polysaccharides is the foundation for the biological activity. Polysaccharides with different chemical structures have great differences in biological activity. The exploration of the structure-activity relationship of plant polysaccharides has guiding significance for the development of new drugs and the health care products of saccharides. Many biological activities of plant polysaccharides such as anti-hepatic injury, antineoplastic, antiviral, hypoglycemia, anticoagulation, anti-oxidation and immunoregulation have been discovered. Therefore, this paper focuses on the bioactivity as the main line, and summarizes the studies on structure-activity relationship and mechanism of plant polysaccharides at home and abroad in recent years. The key point is the structure-activity relationship between plant polysaccharides and the anti-hepatic injury, antineoplastic, antiviral, hypoglycemia, and anticoagulation activities, providing reference for intensive study and exploration of structure-activity relationship of plant polysaccharides as well as development and application of polysaccharides products.

Characterization and activity effect on ADH of polysaccharides from Mori Fructus / 中国中药杂志

Mori Fructus polysaccharides (MFPs) have been used as a source of therapeutic agents. The most promising activities of these biopolymers are their immunomodulation and anti-cancer effects. It was reported that polysaccharides were a potential drug against liver injury, but the hepatoprotective effect of MFPs was ambiguous. In this study, the fractionation of crude polysaccharides on DEAE-Cellulose 52 gave four fractions (MFP-1, MFP-2, MFP-2 and MFP-4). The results showed that the contents of carbohydrates were 75.3%, 83.7%, 79.1%, 74.3%, and the molecular weight of them were 112.2, 128.8, 199.5, 181.9 kDa. Monosaccharide component analysis indicated that MFP-1 was composed of galactose, glucose, arabinose, rhamnose and mannose with the molarity rate of 26.8∶20.4∶8.74∶5∶1; MFP-2 contained arabinose, galactose, rhamnose, glucose and galacturonic acid with the molarity rate of 34.2∶38.2∶8∶17.5∶15.1; MFP-3 was composed of galacturonic acid, galactose, glucose, rhamnose and arabinose with the molarity rate of 28.3∶22.6∶20.9∶18.6∶15.1; MFP-4 contained glucose, galactose, rhamnose, galacturonic acid, arabinose and glucuronic acid with the molarity rate of 47.4∶34.9∶36.1∶33.1∶19.9∶4.1. IR analysis's results indicated that MFP-3 and MFP-4 may be polysaccharides containing β-glycosides. The alcohol dehydrogenase (ADH) activity text showed that, all these four MFPs were found have a positive effect on the activity of ADH, with order: bifendate>MFP-3>MFP-1>MFP-2>tioprnin>MFP-4, and the MFP-3 had the highest activity and demonstrated outstanding hepatic protecting activity.

Advance in study on hepatoprotective effects and its mechanism of polysaccharides / 中国中药杂志

Liver damage is the pathologic status in the liver system, which can lead to cirrhosis, fibrosis and cancer of the liver. So to search for effective drugs on prevention and treatment of anti-hepatic lesion have already attracted broad concern in the world. Polysaccharides widely exist in plants, microorganisms and animals, and have high efficiency but low toxicity properties. Therefore, polysaccharides had been the hotspot in the research field of liver-protecting medicines, and had undergone great progresses. In this study, the hepatoprotective effects and its mechanism of polysaccharides were summarized and reviewed, and the market prospects of development and application were prospected. The research result indicated that polysaccharides derived from plants, microorganisms, animals which had significant effects on liver protection. Results also showed that polysaccharides showed the protective effect on chemical liver injury by anti-oxidation, alleviating calcium overload, adjusting the function of mitochondria, and the protective effect on immune liver injury by regulating cytokine secretion, blocking the complement system activity, inhibiting inflammatory mediator expression, suppressing hepatocyte apoptosis. Polysaccharides had rich resource, diverse bio-effects, abundance approaches and multitarget. Therefore, there is huge potential for developing polysaccharides as novel hepatoprotective medicine candidates.

Study on quality standard of Eucommia ulmoides based on absorbed active components in rat plasma / 中国中药杂志

The method was established for determination of geniposidic acid, chlorogenic acid, pinoresinoldiglucoside, which are three kinds of constituents of Eucommia ulmoides absorbed into the blood components. LC-MS/MS technique was applied to determine the blood components of the bloodstream after administration of E. ulmoides extract. At the same time, HPLC was used for detection of the ingredients content of the blood sample from 23 batches of E. ulmoides. The results showed that geniposidic acid, chlorogenic acid and pinoresinoldiglucoside are prototype into the blood in rats after oral administration of E. ulmoides extract, The linear range of geniposidic acid, chlorogenic acid and pinoresinoldiglucoside was good, and the average recoveries geniposidic acid, chlorogenic acid and pinoresinoldiglucoside were 98.69%, 100.8% and 98.39%, respectively. The method is simple and feasible with good reproducibility.

Study on correlation between plasma NT-pro BNP level and heart function in children with heart failure / 国际检验医学杂志

Objective To investigate the relationship between the plasma NT-pro BNP level and the hear function in children pa-tients with heart failure.Methods 30 children patients with heart failure and 30 healthy children were selected as the patients group and the control group respectively.The plasma NT-pro BNP level was detected in the patients group before and after treatment and the control group.The changes of heart functional indexes and the clinical symptoms in the patients group were detected,the detec-tion results and the plasma NT-pro BNP levels were performed the correlation retrospective analysis.Results The plasma NT-pro BNP level before treatment in the patients group was significantly higher than that in the control group,there was the statistically significant difference between them(P <0.05 ).After treatment,the clinical symptoms in the patients group were obviously im-proved,the plasma NT-pro BNP level was significantly decreased and LVEF by ultrasound was also increased,the differences were statistically significant(P <0.05).The plasma NT-pro BNP level after treatment in the patients group was obviously higher than that in the control group with statistical difference between them(P <0.05).The plasma NT-pro BNP level before and after treat-ment in the patients group demonstrated a negative correlation with LVEF(r=-1.32,r=-1.78).Conclusion Detecting the plas-ma NT-pro BNP level in children patients with heart failure has the important clinical guidance significance for the diagnosis,disease condition evaluation,prognostic evaluation and cardiac function grading in the children patients with heart failure.

Relationships between genetic polymorphisms of triggering receptor expressed on myeloid cells-1 and septic shock in a Chinese Han population / 世界急诊医学杂志（英文）

@#BACKGROUND: Triggering receptor expressed on myeloid cells-1 (TREM-1) is a cell surface receptor expressed on neutrophils and monocytes. TREM-1 acts to amplify inflammation and serves as a critical mediator of inflammatory response in the context of sepsis. To date, the predisposition of TREM-1 gene polymorphisms to septic shock has not been reported. This study was designed to investigate whether TREM-1 genomic variations are associated with the development of septic shock. METHODS: We genotyped two TREM-1 single nucleotide polymorphisms (SNPs, rs2234237 and rs2234246) and evaluated the relationships between these SNPs and septic shock on susceptibility and prognosis. RESULTS: TREM-1 rs2234246 A allele in the promoter region was significantly associated with the susceptibility of septic shock in recessive model (AA, OR=3.10, 95％Cl 1.15 to 8.32, P=0.02), and in codominant model (AG, OR=0.72, 95％Cl 0.43–1.19, P=0.02; AA, OR=2.71, 95％Cl 1.00–7.42;P=0.03). However, in three inherited models (dominant model, recessive model, and codominant model), none of the assayed loci was significantly associated with the prognosis of septic shock. The non-survivor group demonstrated higher plasma IL-6 levels (99.7±34.7 pg/mL vs. 61.2±26.5 pg/mL, P<0.01) than the survivor group. Plasma concentrations of IL-6 among the three genotypes of rs2234246 were AA 99.4±48.9 pg/mL, AG 85.4±43 pg/mL, and GG 65.3±30.7 pg/mL (P<0.01). The plasma concentrations of IL-6 in patients with AA genotypes were significantly higher than those in patients with GG genotypes (P<0.01). CONCLUSION: TREM-1 genetic polymorphisms rs2234246 may be significantly correlated only with susceptibility to septic shock in the Chinese Han population.

Mini-open trans-spatium intermuscular versus percutaneous short-segment pedicle fixation for the treatment of thoracolumbar mono-segmental vertebral fractures / 中国骨伤

<p><b>OBJECTIVE</b>To compare the clinical effects and radiographic outcomes of mini-open trans-spatium intermuscular and percutaneous short-segment pedicle fixation in treating thoracolumbar mono-segmental vertebral fractures without neurological deficits.</p><p><b>METHODS</b>From August 2009 and August 2012, 95 patients with thoracolumbar mono-segmental vertebral fractures without neurological deficits were treated with short-segment pedicle fixation through mini-open trans-spatium intermuscular or percutaneous approach. There were 65 males and 30 females, aged from 16 to 60 years old with an average of 42 years. The mini-open trans-spatium intermuscular approach was used in 58 cases (group A) and the percutaneous approach was used in 37 cases (group B). Total incision length, operative time, intraoperative bleeding, fluoroscopy, hospitalization cost were compared between two groups. Visual analog scale (VAS) and radiographic outcomes were compared between two groups.</p><p><b>RESULTS</b>All patients were followed up from 12 to 36 months with an average of 19.6 months. No complications such as incision infection, internal fixation loosening and breakage were found. In group A, fluoroscopy time was short and hospitalization cost was lower than that of group B (P<0.05). But the total incision length in group B was smaller than that of group A (P<0.05). There was no significant differences in operative time, intraoperative bleeding, postoperative VAS and radiographic outcomes between two groups (P>0.05). Postoperative VAS and radiographic outcomes were improved than that of preoperative (P<0.05).</p><p><b>CONCLUSION</b>The mini-open trans-spatium intermuscular and percutaneous short-segment pedicle fixation have similar clinical effects and radiographic outcomes in treating thoracolumbar mono-segmental vertebral fractures without neurological deficits. However, in this study, the mini-open trans-spatium intermuscular approach has a short learning curve and more advantages in hospitalization cost and intraoperative radiation exposure times, and is recommendable.</p>

Correlation analysis on plasma D-dimer level with deep venous thrombosis after spinal surgery / 中国骨伤

<p><b>OBJECTIVE</b>To analyze the relation of plasma D-dimer levels and incidence of deep venous thrombosis after spinal surgery.</p><p><b>METHODS</b>The clinical data of 63 patients underwent spinal surgery from October 2009 to October 2010 were retrospective analyzed. There were 40 males and 23 females with an average age of 48 years old(21 to 76) in operation. Operation levels of 15 cases were in cervical vertebrae, 4 cases were in thoracic vertebrae,and 44 cases were in lumbar vertebrae. Thirty patients with spinal fracture were caused by trauma and 33 patients without trauma, 11 patients combined with nerve injury. The patients were divided into two groups according to plasma D-dimer levels, more than or equal to 500 microg/L was D-dimer positive group and less than 500 microg/L was D-dimer negative group. Venous blood of all patients early morning with empty stomach were testd on admission, and at 2 h, 1 d, 2 d, 3 d, 4 d, 6 d, 8 d, 10 d, 15 d after operation,respectively.</p><p><b>RESULTS</b>There was no statistically significant differences in sex, operative segments, implants, operative posture, age, bleed volume, body weight, peroperative D-dimer levels between two groups. After operation, plasma D-dimer of 19 patients were more than or equal to 500 microg/L, with persistent or progressive increasing. Two cases occurred deep venous thrombosis in D-dimer positive group, they respectively were found at 3 days and 8 days after operation. Both of them underwent posterior decompression and internal fixation. However,no deep venous thrombosis was found in D-dimer negative group.</p><p><b>CONCLUSION</b>Postoperative D-dimer assay can effective predict deep venous thrombosis occurrence. D-dimer level more than or equal to 500 microg/L will be considered as a risk factor for deep venous thrombosis after spinal surgery.</p>

Dose-response and HPLC spectrum-activity relationships of the anti-inflammatory active site of Kalimeris indica / 中国药学杂志

OBJECTIVE: To explore the dose-effect and fingerprint-effect relationships of the active site of Kalimeris indica. METHODS: The HPLC fingerprints of the active site at different doses were determined. Mouse auricle swelling model was used to observe the anti-inflammatory action of Kalimeris indica extract and its dose-response relationship. Grey correlation method was used to assess the relationship between the fingerprint peaks and effects. RESULTS: The was a positive correlation between the dose and anti-inflammatory activity of the extract (r=0.9511). The extract had 18 common peaks, among which four were identified, and the comprehensive correlation between the common peaks and anti-inflammatory activity was from 0.5608 to 0.9599, with ranking of 10> 14> 5> 8> 15> 9> 2> 7> 12> 1> 17> 3> 18> 16> 6> 13> 4> 11. CONCLUSION: The spectrum-activity relationship of Kalimeris indica established in this study could provide theoretical basis and data support for the quality control pattern of traditional Chinese medicines.

HPLC fingerprint of ethyl acetate extraction of Saxifraga stolonifera / 中国中药杂志

<p><b>OBJECTIVE</b>To establish an HPLC fingerprint of ethyl acetate extraction of Saxifraga stolonifera.</p><p><b>METHOD</b>The HPLC analysis was performed on a Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column with isocratic elution of acetonitrile-0.05% phosphoric acid at a flow rate of 1.0 mL x min(-1). The detection wavelength was set at 256 nm and the column temperature was set at 30 degrees C.</p><p><b>RESULT</b>The HPLC fingerprint of ethyl acetate extraction of S. stolonifera has been established. There were fifteen common peaks, seven of which were identified by reference substances. The RSD of relative retention time was less than 3% in the precision and repeated tests. Eleven samples from different area can be distinguished from their fingerprints.</p><p><b>CONCLUSION</b>This method is reasonable and reliable and can be used for quality control of S. stolonifera.</p>