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BACKGROUND: Although considerable progress has been made in spinal tuberculosis surgery,considering the particularity of the spine position, the surgical treatment is limited by the body stress and secondary injury due to removal of the internal fixation. Tissue engineering provides a new insight into the treatment of various diseases.Ideal biomaterials instead of traditional internal fixation materials are characterized by non-toxic and non-immune rejections.OBJECTIVE: To explore the biocompatibility and mechanical properties of alginate combined with bone marrow mesenchymal stem cells for repair of spinal tuberculosis.METHODS: Forty New Zealand rabbits were divided into four groups, 10 rats in each group: control group, spinal tuberculosis group, alginate group and titanium alloy group. The control group received no treatment, and in the other groups, boreholes were drilled in the fifth lumbar intervertebral disc and filled with gelatin sponge, and Mycobacterium tuberculosis suspension 0.1 mL (the amount of bacteria, 5 g/L)was then injected to make spinal tuberculosis models.Two months after modeling, simulated L4-5 spinal tuberculosis surgery was performed in the latter two groups, and alginate combined with bone marrow mesenchymal stem cells and titanium alloy internal fixation material were implanted,respectively.RESULTS AND CONCLUSION: One rabbit in the titanium alloy group had local infection and swelling. No rabbit in the alginate group had adverse reactions, such as swelling and local infection. Anteflexion, rear protraction, lateral bending distance and maximum axial pullout force in the alginate group and titanium alloy group were superior to those in the spinal tuberculosis group (P 0.05). The above results showed that the alginate gel combined bone marrow mesenchymal stem cells has good biocompatibility with the body after implantation,and the post-implantation spinal stability is good and close to that after implantation of the titanium alloy.
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BACKGROUND:Insufficient source of seed cel s is the important factor to restrict the tissue reconstruction and the development of regenerative medicine. OBJECTIVE:To explore the osteogenesis of adipose stem cel s cultured with different kinds of artificial bones. METHODS:Adipose tissue was extracted from female volunteers undergoing cosmetic surgery to isolate adipose stem cel s. Passage 4 adipose-derived mesenchymal stem cel s were selected and divided into osteogenic induction group, osteogenic induction+hydroxyapatite group, osteogenic induction+absorbable bone group and osteogenic induction+recombinant bone xenograft group. The latter three groups were subdivided into 3, 10, 20 g/L subgroups, respectively. Culture medium was exchanged every 3 days, total y for 12 days. RESULTS AND CONCLUSION:Compared with the osteogenic induction group, the calcium concentration in the elution liquid was significantly higher in the osteogenic induction+hydroxyapatite group and low-concentration osteogenic induction+absorbable bone group (both P<0.05), but no difference was found between the osteogenic induction group and high-concentration osteogenic induction+absorbable bone group (P<0.05). In addition, the calcium concentration in the elution liquid was significantly lower in the osteogenic induction+recombinant bone xenograft group than the osteogenic induction (P<0.05). Therefore, different artificial bone scaffolds can influence the osteogenic effect of adipose stem cel s, and among them, hydroxyapatite has a better effect on the osteogenic induction of adipose stem cel s.
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BACKGROUND: Now, dual-energy X-ray absorptiometry is international y recognized as gold standard for the diagnosis of osteoporosis, but the errors can be found in the measurement results due to the heterotopic ossification and bone hyperplasia exists in the measurement part. OBJECTIVE: To investigate the clinical significance of bone metabolic markers in the diagnosis and treatment of elderly patients with osteoporotic fractures, and to research its correlation with the changes of pathological histology and bone mineral density. METHODS: Four bone biochemical markers in 50 elderly patients with osteoporosic fractures were measured preoperatively. According to the results, 25 patients had significantly increased tartrate-resistant acid phosphatase 5b (considered as the increased tartrate-resistant acid phosphatase 5b group), and 25 patients had increased bone alkaline phosphatase (considered as the increased bone alkaline phosphatase group). During operation, the bone tissues of eight patients in each group were treated with hematoxylin-eosin staining and electron microscopy scanning in order to detect the pathological changes. After operation, the patients in the increased tartrate-resistant acid phosphatase 5b group received salmon calcitonin anti-osteoporosis treatment, and the patients in the increased bone alkaline phosphatase group received the anti-osteoporosis treatment of bone peptide injection. The bone mineral density and the four bone biochemical markers were detected again at 6 months after treatment. RESULTS AND CONCLUSION: There were no significant differences in the preoperative bone mineral density and four biomechanical markers between two groups (P > 0.05). The pathological examination results of bone tissue on the fracture site showed that the number of osteoblasts was reduced and the number of oeteoclasts was increased in the increased tartrate-resistant acid phosphatase 5b group; while in the increased bone alkaline phosphatase group, the pathological examination results showed the number of osteoblasts was reduced; the trabecular bone/bone area ratio was decreased in two groups, and there was a significant difference in the decrease degree between two groups (P < 0.05). The electron microscope scanning showed that the osteoclasts of two groups were more active than that of the normal group. The sloppy of trabecular bone in the increased tartrate-resistant acid phosphatase 5b group was more obvious than that in the increased bone alkaline phosphatase group, and the absorption vacuoles were increased. There were significant differences in the bone mineral density and four biomechanical markers between two groups before and after anti-osteoporosis treatment (P < 0.05). The detection of bone metabolic markers could help us to make it clearly that the main function of osteoblast reduce or osteoclast increase in bone tissue of patients, and guide us to use anti-osteoporosis drugs in target. Pathological histology examination can better reflect the condition of osteoblasts, osteoclasts and trabecular bone in bone tissue on the fracture site. Target application of anti-osteoporosis drugs in the osteoporosis patients can effectively improve the efficacy and reduce the relative complications.
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Conformations of surface atoms in various stages of nanogold-based genechip testing were scanned by the atomic force microscope based on the scanning tunneling microscope. The findings were: First, the surface atoms of genechip slide (formylphenyl glass) were in a regular porous-arrangement; Second, after combination with probe, the regular porous arrangement changed to be irregular; Third, after hybridization with the target nucleic acid, the surface atoms were once again in a cable-like arrangement which was relatively structured and intensively cross-parallel. However, after the silver staining, the surface atoms showed a larger block structure with serious unevenness. From these results we can intuitively know the process and differences in probe combination, nucleic acid hybridization, and silver staining. Moreover, the relevant experiment was verified at the micro-level.
Subject(s)
Gold , Metal Nanoparticles , Chemistry , Microscopy, Atomic Force , Methods , Microscopy, Scanning Tunneling , Methods , Molecular Conformation , Nanotechnology , Methods , Oligonucleotide Array Sequence Analysis , Methods , Particle Size , Surface PropertiesABSTRACT
The surface plasmon resonance (SPR)-based gene chip was prepared according to the following processes: First, a film of nanogold, which was synthesized by using Frens' method, was plated on chip by Chlorauric acid/hydroxylamine method. Then probes were fixed on nanogold film by Self-assembled monolayer (SAM) technology. Subsequently, the fixing time and concentration of probes, the sensitivity and the specificity of the chip were optimized. Our results suggested that the chip plated with 2.5 nm nanogold film has a better SPR reflection, and when fixed by probes for 4.5 h at the concentration of 1 500 nmol/L, the gene chip also shows a fine performance of detection and can identify accurately the mismatch between bases in SPR detection system. The gene chip constructed in the research can be used for SPR sensor detection.
Subject(s)
Gold , Chemistry , Nanoparticles , Chemistry , Neisseria gonorrhoeae , Genetics , Oligonucleotide Array Sequence Analysis , Sensitivity and Specificity , Surface Plasmon Resonance , MethodsABSTRACT
Objective To study application of surface plasmon resonance(SIR)system in detection of clinical pathogen with a gene chip.Methods 27 clinical samples were detected by SPR-based gene chip system.These samples were composed by 8 positive blood samples,3 positive pyoid samples,9 positive leucorrhea samples and positive reproductive tract pyoid samples,1 positive biopsy sample and 6 negative biopsy samples.Specific primers and probes for target pathogens were designed by bioinformatics methods and validated by PCR and enzyme-labelled chemiluminescence,respectively.SPR-based gene chip was prepared and utilized to detect clinical samples by SPR system.Results The primers and probes showed good specificity and accuracy,which can be applied to perform PCR and application of the gene chip.Compared with the clinical analysis,gene chip analysis of 26 clinical samples showed the consistent results.Conclusions SPR detection system proved to be accurate and reliable.The chip will have a promising prospect in application.