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Objective To study the function and mechanism of receptor-interacting protein kinase 3 (RIPK3)-mediated necroptosis in liver ischemia-reperfusion injury (IRI) in mice.Methods Sixty mice were randomly divided into four groups using Stata statistical software:Wild-type (WT)-sham group,WT-IRI group,HKO (HKO:RIPK3 liver-specific knockout)-sham group and HKO-IRI group.Sham operation was used as a control in which only the hepatic portal blood vessels were freed after laparotomy,and blood flow was not blocked.In the WT-IRI group and the HKO-IRI group,the hepatic portal vein was freed,and the blood supply of left hepatic lobe and the mid-hepatic lobe wer blocked for 90 min,then the blood vessels were opened for 6 h.Blood and liver tissue samples of each group of mice were taken to detect liver function.Inflammatory infiltration and liver injury were detected by immunohistochemistry and hematoxylin and eosin (HE) staining,and autophagyassociated protein LC3-Ⅱ and P62 were detected by Western blotting.The primary hepatocytes of WT mice and HKO mice were extracted and divided into control group and hypoxia-reoxygenation group (HIR group).After attachment of primary hepatocytes,the HIR group was given hypoxia for 6 h and reoxygenated for 4 h.The supernatant was taken for detecting ALT and AST,and the cell extract protein was used to detect LC3-Ⅱ and P62.Results As compared with the control groups,the liver functions of the IRI groups were significantly impaired,and as compared with the WT-IRI group,the liver damage was significantly aggravated in the HKI-IRI group (P ＜ 0.05),and the LC3-Ⅱ protein content was significantly decreased and the P62 protein content was increased.Similarly,after hepatocytes were were given hypoxia and reoxygenated,HKO-derived hepatocytes were more severely damaged than WT-derived hepatocytes.Conclusions Blocking RIPK3-mediated necroptosis of hepatocytes could induce autophagy inhibition,which aggravates hepatic ischemia-reperfusion injury.
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Objective To investigate the correlation between type 2 diabetes mellitus (T2DM)and hepatocellular carcinoma (HCC)by case-control study. Methods From January 2006 to December 2015,the data of 1350 first diagnosis HCC patients at the First Affiliated Hospital of Zhengzhou University were collected and analyzed. The univariate and multivariate logistic regression were performed to analyze the risk factors of HCC genesis,and the risk factors were further stratified. Results The results of univariate logistic regression analysis showed that smoking and obesity were not risk factors of HCC genesis (both P > 0. 05). Alcoholism, hepatitis B virus (HBV)infection,hepatitis C virus (HCV)infection,T2DM,and family history of HCC were the risk factors of HCC genesis (odds ratio (OR)= 2. 593,4. 583,3. 732,1. 955 and 1. 622,95％ confidence interval (CI)1. 883 to 3. 549,3. 026 to 6. 940,2. 544 to 5. 367,1. 708 to 2. 477 and 1. 314 to 2. 267,all P < 0. 05). The results of multivariate logistic regression analysis indicated that alcoholism,HBV infection, HCV infection,T2DM and family history of HCC were independent risk factors of HCC genesis (OR = 2. 034, 4. 564,2. 831,1. 881 and 1. 616,95％ CI 1. 507 to 2. 745,2. 672 to 7. 765,1. 562 to 5. 047,1. 314 to 2. 671 and 1. 177 to 2. 228,all P < 0. 01),and T2DM could increase the risk of HCC genesis (P < 0. 01). In order to exclude the interference of alcoholism,HBV infection and HCV infection on above results,further stratified analysis showed that T2DM was one of the independent risk factors of HCC genesis (χ2 = 5. 190,P = 0. 023), and had synergistic effect with alcoholism,HBV infection and HCV infection (χ2 = 32. 848,P < 0. 01). There were significant differences in the propovtion,duration of disease and hemoglobin A 1c level of T2DM patients between the case group and the control group (χ2 = 46. 618,81. 644 and 43. 092,all P < 0. 01). Conclusions T2DM is one of the independent risk factors or HCC genesis. T2DM is correlated with the pathogenesis of HCC. The clinical desease course and blood glucose control in patients with T2DM are also correlated with the pathogenesis of HCC.
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Objective@#To investigate the correlation between type 2 diabetes mellitus (T2DM) and hepatocellular carcinoma (HCC) by case-control study.@*Methods@#From January 2006 to December 2015, the data of 1 350 first diagnosis HCC patients at the First Affiliated Hospital of Zhengzhou University were collected and analyzed. The univariate and multivariate logistic regression were performed to analyze the risk factors of HCC genesis, and the risk factors were further stratified.@*Results@#The results of univariate logistic regression analysis showed that smoking and obesity were not risk factors of HCC genesis (both P>0.05). Alcoholism, hepatitis B virus (HBV) infection, hepatitis C virus (HCV) infection, T2DM, and family history of HCC were the risk factors of HCC genesis (odds ratio (OR)=2.593, 4.583, 3.732, 1.955 and 1.622, 95% confidence interval (CI) 1.883 to 3.549, 3.026 to 6.940, 2.544 to 5.367, 1.708 to 2.477 and 1.314 to 2.267, all P<0.05). The results of multivariate logistic regression analysis indicated that alcoholism, HBV infection, HCV infection, T2DM and family history of HCC were independent risk factors of HCC genesis (OR=2.034, 4.564, 2.831, 1.881 and 1.616, 95%CI 1.507 to 2.745, 2.672 to 7.765, 1.562 to 5.047, 1.314 to 2.671 and 1.177 to 2.228, all P<0.01), and T2DM could increase the risk of HCC genesis (P<0.01). In order to exclude the interference of alcoholism, HBV infection and HCV infection on above results, further stratified analysis showed that T2DM was one of the independent risk factors of HCC genesis (χ2=5.190, P=0.023), and had synergistic effect with alcoholism, HBV infection and HCV infection (χ2=32.848, P<0.01). There were significant differences in the propovtion, duration of disease and hemoglobin A1c level of T2DM patients between the case group and the control group (χ2=46.618, 81.644 and 43.092, all P<0.01).@*Conclusions@#T2DM is one of the independent risk factors or HCC genesis. T2DM is correlated with the pathogenesis of HCC. The clinical desease course and blood glucose control in patients with T2DM are also correlated with the pathogenesis of HCC.
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ObjectiveTo investigate the association of HLA-Cw alleles with inflammatory bowel disease(IBD), so as to identify IBD susceptibility gene.MethodsThe HLA-Cw genotype were analyzed in 100 patients with ulcerative colitis (UC), 73 patients with Crohn's disease (CD) and 106 randomly ethnically matched healthy controls by sequence specific primer polymerase chain (PCR-SSP).Results HLA-Cw * 07 gene phenotype frequencies increased in patients with UC (0.430) compared with that in healthy controls (0.226), P = 0.002; while HLA-Cw * 12 gene phenotype frequencies increased in patients with CD (0.356) compared with that in healthy controls (0.123), P = 0.000.ConclusionHLA-Cw * 07 allele and HLA-Cw * 12 allele may be strongly associated with the susceptibility of UC and CD, respectively.
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Objective To explore the value of ultrasound in diagnosis and classification of pilomatricoma.Methods Ultrasonic features of 63 cases with pilomatricoma confirmed by operation and pathology were analyzed and summarized retrospectively.Results According to if there was calcification in the tumor,pilomatricoma cases were classified into two groups(calcific group and non-calcific group).In addition,there were three subgroups in the calcific group based on the size and disposition of calcification:subgroup Ⅰ,completely echogenic mass with strong posterior acoustic shadowing in the subcutaneous layer (n=17,26.98%);subgroupⅡ,hypoechoic nodules with tiny calcification diffused distributed(n=15,23.80%);subgroup Ⅲ,hypoechoic nodules with large calcification(n=8,12.70%).Twenty-three cases (36.51%)were distributed into non-calcific group.Conclusions Ultrasonography is valuable for diagnosis and differential diagnosis of pilomatricoma,and it is an essential examination method for the tumor before operation.
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Objective To explore the effect of adriamycin, bleomyein, vincristine and dacarbazinum (ABVD) chemotherapy scheme executed at day I and day 8 for primary Hodgkin's lymphomas (HL). Methods 62 patients with primary HL in stages Ⅱ-Ⅳ treated in our department from October 2005 to October 2006 were divided into group A and B at random with 31 patients in each group. The patients in group A received ABVD chemotherapy scheme executed at day 1 and day 8 for 6-8 cycles. The patients in group B received ABVD chemotherapy scheme executed at day 1 and day 15 for 6-8 cycles. The patients of the groups received radiotherapy by the same doctor after chemotherapy according to the patients condition and the radiotherapy regimens were not affected by the grouping. Results The complete remission rate (CR)in group A after chemotherapy was 90. 3% (28/31);the one-year and two-year disease free survival (DFS) rates were 87. 1% (27/31) and 80.0% (20/25)respectively. The CR rate in group B after chemotherapy was 83.9% (26/31);the one-year and two-year DFS rates were 80. 6% (25/31)and 72. 0% (18/25) respectively. The discrepancy of CR rates and the one-year and two-year DFS rates between the two groups was not significant (P>0.05). The incidences of therapeutic side effecte such as myocardial iscnemia grade Ⅲ-Ⅳ liver function impair-ment,pulmonary fibrosis and serious marrow inhibition between the two groups were not significant too (P > 0.05). Average chemotherapy period for the patients in group A was 159 days; it was 69 days shorter than that in group B. Conclusion The CR rate,1-year DFS rate and 2-year DFS rate of ABVD chemotherapy scheme executed at day 1 and 8 are similar to those of ABVD chemotherapy scheme executed at day Ⅰ and 15 for primary HL in stages Ⅱ-Ⅳ. The side-effects of chemoterapy between group A and B are similar too. The chemotherapy period in group A is shortened significantly.
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Objective To evaluate the value of high-frequency ultrasound and X-ray barium meal examination in diagnosis and typing diagnosis of congenital hypertrophic pyloric stenosis(CHPS).Methods High-frequency ultrasound and X-ray barium meal examination were made in newborns with present symptom of vomiting,and comparison was made between the two examine methods.Retrospective analysis was made in 29 cases confirmed by surgical operation.Control normal group included twenty healthy newborns.Results In 29 CHPS cases,the diagnostic accordance rate of ultrasound was 1 00%.And the diagnostic accordance rate of X-ray barium meal examination was 93.1%(27/29).In all those CHPS cases,the length of pyloric canal were≥18 mm,the diameter of pyloric canal were≥14 mm,the wall thickness of pyloric canal were≥4 mm,the stenosis inside diameter were≤6 mm by ultrasound;in X-ray barium meal examination,the length of pyloric canal were≥20 mm,the inside diameter were≥7 mm,and there was significant difference in comparison with control group(P＜0.01).The CHPS was classified into two groups as mild stenosis and severe stenosis according to the inside diameter and the wall thickness of pyloric canal.The mild type showed the wall thickness was＞4 mm and≤6 mm,the inside diameter was＞3 mm;The severe type showed the wall thickness was＞6 mm,the inside diameter was≤3 mm or obliterate.Conclusions High-frequency ultrasound has significant clinical application value in diagnosis and subtype diagnosis of CHPS,it is an easy,reliable and safe examination method.
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Objective To investigate the gene polymorphism of inhibitory killer cell immunoglobulin- like receptor (iKIR) in patients with inflammatory bowel disease (IBD) and whether the iKIR gene polymorphisms were associated with IBD.Methods Peripheral blood DNA samples were isolated from 100 patients with ulcerative colitis (UC),52 patients with Crohn's disease (CD) and 106 randomly ethnically matched healthy controls.The iKIR gene polymorphisms were analyzed by sequence specific primer polymerase chain reproduction (PCR-SSP).Phenotypic frequency and gene frequency of iKIR gene were calculated,and differences were compared between IBD patients and healthy controls.Results iKIR genes (including KIR2DL1,KIR2DL2,KIR2DL3,KIR2DL4,KIR2DL5,KIR3DL1,KIR3DL2, KIR3DL3) were found to be present in all subjects at different levels.Interestingly,phenotypic frequencies of KIR2DL1 and KIR2DL3 were significantly lower in UC patients than those in healthy controls (P = 0.001),while phenotypic frequencies of KIR2DL2,KIR2DL4,KIR2DL5,KIR3DL1, KIR3DL2 and KIR3DL3 were no difference between UC patients and healthy controls (P>0.05).The phenotype frequency of KIR2DL1 was significantly decreased in CD patients compared with healthy controls (P = 0.007),while phenotypic frequencies of other iKIR were observed to be no significant change between CD patients and healthy controls (P>0.05 ).Conclusions The KIR2DL1 and KIR2DL3 gene phenotype frequencies are decreased in UC patients,which suggests that these gene polymorphisms are associated with the susceptibility of UC,and the polymorphism of KIR2DL1 gene is involved in the susceptibility of CD.
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Objective To study the sonographic features of solid-pseudopapillary tumors of the pancreas(SPTP) and to explore the value of ultrasound in the dignosis,differential dignosis and type of SPTP.Methods B-mode ultrasonography and color Doppler flow imaging(CDFI) of nine cases of SPTP confirmed by clinical and pathology were enrolled in the study and some cases were compared with the findings of computed tomography (CT).Results On B-mode ultrasound images,tumors showed solid,solid and cystic alternative distributions or cystic structures,presenting as round or oval shape,well-defined margins.Of these nine lesions,one was mainly composed of solid part,seven showed solid tumor with cystic component inside and one showed cystic tumor.All tumors were encapsulated.The encapsulation was completive in seven cases,incontinuous in one case and interruptive in one case.In CDFI,blood flow was found in five cases, three cases were I-brand,one case was II-brand,one case was III-brand.The peak systolic velocity ranged from 8.6 cm/s to 33.2 cm/s,three cases were less than 20 cm/s.Resistance index(RI) varied from 0.38 to 0.56 and three cases were lower than 0.5.According to the sonographic features of SPTP,combined with related reviews about the tumor,those masses were classified into three types as homogeneous solid,solid and cystic alternative distributions and cystic tumor.Conclusions The B-mode ultrasound and CDFI characteristic findings might be of great value in dignosis and differential dignosis of SPTP.
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Purpose To investigate the effects of different concentration of ketamine on Ca2 transsarcolemmalinflux induced by KCl in isolated rat ventricular myocytes. Methods Freshly isolated rat ventricular nyoeyteswere loaded with Fluo-3AM, a Ca2 + indicator. The effects of different concentration ketamine( 1 × 10- s, 1 × 10- 4,1 × 10-3 mmol/L) on the change of intracellular Ca2+ concentration induced by KCl were investigated. ResultsLow concentration ketamine(1 × 10-5 mrnol/L) did not change Ca2+ transsarcolemmal influx. Although mediumeoncentration ketamine( 1 × 10-4 rmol/L) made the influx slower, the eventual peak concentration of intracellularCa2+ had no difference from that of the control group. The high concentration ketamine (1 × 10-3 mmol/L) inhibited Ca2-1 influx,intracellular Ca2+ fluorescent intensity decreased about 13.2% (P＜0.05). ConclusionsKetamine inhibits Ca2 + trranssarcolemmal influx in isolated rat ventricular myocytes dosedependently, which may inpart explain its negative inotropic effect.
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Objective To study the status of cystatin B gene expression in human esophageal cancer. Methods Expression of cystatin B gene was detected by RT PCR in 41 pairs of human esophageal cancer tissues, matched adjacent normal mucosa, human esophageal cancer cell line EC9706 and human lung cancer cell line GLC82. Results cystatin B gene expression was down regulated in 82.9% (34/41) of human esophageal cancer tissues while expressed at high level in all matched adjacent normal esophageal mucosa, and was significantly corelated to lymph node metastasis ( P
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Objective: To examine the effects of midazolam-induced sedation on heart rate variability (HRV). Method:Fifteen ASA Ⅰ- Ⅱ adult patients,undergoing elective surgery under lumbar epidural anesthesia were randomly selected. An intravenous bolus dose of midazolam(1.5mg) was administered every 3-5 minutes until patients' sedation levels assessed by observers assessment of alertness sedation(OAA/S) scale had scores of 1. Spectral analysis of HRV was performed at different OAA/S scores and at 3min,5min and 10min following OAA/S score of 1. Result:All frequency components of HRV were significantly reduced as patients' OAA/S scores decreased,especially low frequency (LF) and total power. Midazolam decreased normalized unit power of LF from 33.5%?8.9% to 16.65?9.6% and increased normalized unit power of high frequency(HF) from 11.7%?4.2% to 20.5%?26.5%. LF/HF ratio also reduced. Conclusion:Midazolam shiftes the balance of autonomic nervous activity toward the parasympathotonic.
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Objective To investigate the mechanisms of Mal gene down regulated in human esophageal cancer. Methods We amplified the 5′regulatory region of Mal gene in normal placenta tissue and human esophageal cancer cell lines EC9706、EC8712、EC109 by optimization the PCR methods, cloned and sequenced the PCR products and compare the sequence. Results Human esophageal cancer cell lines EC9706、EC8712、EC109 all have the 654 th of 5′regulatory region of Mal gene T to C changes, corresponding codon changed from TCC to CCC, and the amino acid which it codes changed from serine to proline; EC9706 also has the 657 th of 5′regulatory region of Mal gene T to C changes, corresponding codon changed from TGC to CGC, the amino acid which it codes changed from cysteine to arginine; EC8712 has the 139 th of 5′regulatory region of Mal gene A to G changed, corresponding codon changed from GGA to GGG, the amino acid which it codes remains glycine. Conclusion Our results suggested there are may point mutation in 5′ region of Mal gene in human esophageal cancer.
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Objective: To analyse correlation of bispectral index,spectral edge frequency of electroencephalogram with midazolam-induced sedation. Method: 30ASA grade Ⅰ-Ⅱ adult patients, undergoing elective surgery under regional anesthesia were randomly devided into three groups according to intravenous bolus doses of midazolam,i, e. group Ⅰ:0.05mg?kg~(-1),group Ⅱ:0.1mg?kg~(-1),group Ⅲ:0.2mg?kg~(-1). After an intravenous bolus dose of mida zolam was administered,both bispectral index (BIS), 95% spectral edge freguency (SEF) of electroencephalogram were monitored and their correlation with midazolam induced sedation was analysed. Result: Both BIS and 95% SEF-correlated with midazolam-induced sedation significantly (r= 0.86,0.73, P
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ObjectiveTo investigate the clinical appli cation of ultrasound in Peyronie disease. MethodsSixty-nine patients with Peyronie disease were examined by conventional ultrasound, and compared with pathology. ResultsUltrasonographic imaging showed the size and morphological of Peyronie plague,and located the position of the plagues.The ultrasound figures of plagues directly related to pathological components and case history,and they were roughly divided into twe types:hypoechoic type and hyperechoic type.ConclusionsUltrasound examination is the best and reliable method in choice for Peyronie plagues and evaluation of the therapeutic effects.
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The effects of continuous occlusion of thoracic or abdominal aorta (OTA or OAA) via intra-aortic balloon inflation on coronary perfusion pressure (CPP) were observed in 12 dogs after 10 min of cardiac fibrillation in comparison with those of different doses of epinephrine (0.02~0.14 mg/kg). The results showed that OTA could not increase CPP. However, during 30 min of cardiopu-Imonary resuscitation(CPR), every 3 min of OAA was followed by markedly increased CPP, 067 ~ 12kPa higher than the value in balloon deflation peritxl right before(P