ABSTRACT
Objective To study the effect and mechanism of capillary microcirculation disturbance on intracerebral hemorrhage. Methods The loading effect of capillaries was replaced by the introduction of porous media. A microcirculation model from the capillaries to the veins was established. The appropriate mechanical boundary conditions were set up for the model by referring to various physiological conditions of human body, and the changes in blood pressure and stress of vascular wall under various conditions were simulated. Results Under normal circumstances, the whole blood pressure of the LSA was relatively low, and the pressure difference between the beginning and the end of the LSA was more obvious, and the stress of all parts of the vascular wall was at the same level. In the case of microcirculation disorder, the whole blood pressure of the LSA increased and the pressure difference between the beginning and the end of the LSA significantly decreased. The stress for each part of the vessel increased and the stress at the end of the LSA increased most significantly. Conclusions The influence of microcirculation disturbance on hemodynamics of the LSA was particularly significant. It was an important factor leading to hemorrhage of the LSA rupture. The research findings are of important theoretical and practical significance for understanding the mechanism of cerebral vascular rupture and preventing the occurrence of cerebral hemorrhage in the case of microcirculation disturbance.
ABSTRACT
Lysostaphin, a specific endopeptidase enzyme derived from Staphylococcus aureus, is a bactericidal agent against Staphylococcus and difficult to be drug-resistant. This study established the monoclonal antibody affinity chromatography to obtain lysostaphin of high purity for drug-use standard. The purified Lysostaphin was of > 95% purity and its recovery rate more than 90%. Moreover, the affinity column kept its efficiency of purification invariable after more than 30 times repeat. Also, the dye release assay validated that the purified lysostaphin had significant bactericidal activity. This method was simple and of high efficacy for the lysostaphin purification and showed its potency in commercial production.
Subject(s)
Antibodies, Monoclonal , Allergy and Immunology , Chromatography, Affinity , Methods , Lysostaphin , Recombinant Proteins , Staphylococcus aureusABSTRACT
Objective:To prepare immunoliposomes encapsulating alkaline phosphatase and to evaluate the methods.Methods:The liposomes containing alkaline phosphatase were prepared by reverse phase evaporation or film dispersion.Liposomes and rabbit anti-human IgG were conjugated by glutaraldhyde or deriving antibody.Not only did the liposomes encapsulate alkaline phosphatase but also they had immune activity.All methods were analyzed and compared.Results:Many liposomes prepared by reverse phase evaporation had perfect shape and high bioactivity of alkaline phosphatase.The glutaraldhyde method conjugating antibody was convenient,efficient and practical.Conclusion:Immunoliposomes with bioactivity of enzyme and antibody are prepared through reasonable methods.