ABSTRACT
Severe aplastic anemia II (SAA-II) progresses from non-severe aplastic anemia (NSAA). The unavailability of efficacious treatment has prompted the need for haploidentical bone marrow transplantation (haplo-BMT) in patients lacking a human leukocyte antigen (HLA)-matched donor. This study aimed to investigate the efficacy of haplo-BMT for patients with SAA-II. Twenty-two patients were included and followed up, and FLU/BU/CY/ATG was used as conditioning regimen. Among these patients, 21 were successfully engrafted, 19 of whom survived after haplo-BMT. Four patients experienced grade II-IV aGvHD, including two with grade III-IV aGvHD. Six patients experienced chronic GvHD, among whom four were mild and two were moderate. Twelve patients experienced infections during BMT. One was diagnosed with post-transplant lymphoproliferative disorder and one with probable EBV disease, and both recovered after rituximab infusion. Haplo-BMT achieved 3-year overall survival and disease-free survival rate of 86.4% ± 0.73% after a median follow-up of 42 months, indicating its effectiveness as a salvage therapy. These promising outcomes may support haplo-BMT as an alternative treatment strategy for patients with SAA-II lacking HLA-matched donors.
Subject(s)
Humans , Anemia, Aplastic/therapy , Bone Marrow Transplantation , Graft vs Host Disease , HLA Antigens , Hematopoietic Stem Cell Transplantation , Transplantation ConditioningABSTRACT
Objective:To explore the effects of different doses of Ethephon on testes of male pups.Methods:Thirty-two 45-day-old healthy female Sprague-Dawley (SD) rats were randomly divided into the control group and the low dose, middle dose and high dose Ethephon groups by the random figure table.The female rats in the low dose, middle dose and high dose Ethephon groups were given 200, 400, and 800 mg/kg Ethephon solution, respectively.The control group was treated with 9 g/L saline.After the birth of the offspring, the mother rats were not administrated with any medications, and the male offspring rats were given Ethephon solution instead.Twelve offspring male rats were randomly selected from each group and killed at the age of 0, 14 and 28 days after birth.Fresh testicular tissues were stained with hematoxylin eosin (HE), and the morphological changes of testicular tissues were observed under light microscope.The apoptotic cells were labeled by terminal dexynucleotidyl transferase (TdT) mediated dUTP nick end labeling (TUNEL) method and the apoptosis index (AI) of spermatogenic cells was detected by fluorescence microscope.Results:(1) Compared with the newborn rats in the middle dose group, low dose group and control group, se-miniferous tubules in the newborn rats of the high dose group were slightly thicker, and seminiferous cells were arranged slightly in disorder.The AI of the newborn rats in high dose group was significantly higher than that in the control group (1.00±0.06 vs.0.41±0.03, P<0.01). The AI of the newborn rats in the middle dose group was not significantly different from that in the control group and the low dose group ( P>0.05). (2) The seminiferous tubules of the 14-day-old rats in the low dose, middle dose and high dose Ethephon groups were significantly thicker and arranged more loosely than those in the control group.Compared with the control group, there were very few seminiferous cells, which were arranged disorderly in the low dose, middle dose and high dose Ethephon groups.The AI of the 14-day-old rats in the low dose, middle dose and high dose Ethephon groups was (2.13±0.10), (2.18±0.10) and (3.90±0.23), respectively, which were significantly higher than that in the control group (1.00±0.02) ( F=2 508.36, P<0.01). There was no significant difference in the AI between the middle dose and low dose groups ( P>0.05). (3) Compared with the control group, the seminiferous tubules of the 28-day-old rats in the low dose, middle dose and high dose groups were significantly thicker and arranged much more loosely, and spermatogenic cells were even less and arranged in a severely disordered way.The AI of 28-day-old rats in the low dose group (5.52±0.13), the middle dose group (9.44±0.07) and the high dose group (14.56±0.27) was significantly higher than that in the control group (3.11±0.13) ( F=10 784.69, P<0.01). Conclusions:Ethephon can thicken the seminiferous tubules of newborn and young rats, cause the germ cells to arrange disorderly, promote the apoptosis of spermatogenic cells and reduce the ability of spermatogenesis.Moreover, a longer exposure of the rats to a higher concentration of Ethephon will result in more serious damage to testicular tissues.
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Objective To investigate the effects of Ethephon on spermatogenic cells and sex hormones levels of adolescent male rats.Methods The male SD rats of 25-day old were randomly (from the random number table) divided into high dose group (2 000 mg/kg),middle dose group (1 000 mg/kg),low dose group (500 mg/kg) and control group (the same amount of physiological saline).They were given Ethephon through stomach for 14 d.The pathological changes in testis tissues were observed by HE staining.The apoptosis of germ cells were detected by terminal transferase labeling (TUNEL).The automatic chemical luminescence immunoassay analyzer was used to test the serum sex hormone levels.Results After ethephon and saline lavage for 14 days in the dose of 2 000 mg/kg,1 000mg/kg,500 mg/kg concentrations respectively,body weight growth was significantly decreased (F =3.58,P =0.03).Testis mass growth [(0.91 ± 0.17) g,(1.13 ± 0.15) g,(1.21 ± 0.11) g,(1.29 ± 0.28) g] was significantly decreased (F =4.31,P =0.02).Experimental group spermatogenic cell apoptosis,the apoptosis index increased (F =156.00,P =0.00),high dose group[(2.40 ±0.18)％] and middle dose group[(1.72 ±0.14)％] was significandy increased (P ＜ 0.01).The levels of testosterone and estrogen in serum showed a decreasing trend along with the increase of the doses,and there was a statistical significance (F =11.85,38.93,all P =0.00).Compared with the control group [(0.86 ± 0.10) μg/L],the testosterone levels in high dose group [(0.31 ± 0.08) μg/L],middle dose group [(0.36± 0.05) μg/L] decreased significantly (P ＜ 0.01).Compared with the control group,low dose group,middle dose group [(36.43 ± 3.57) ng/L,(38.62 ± 2.24) ng/L,(31.87 ± 5.78) ng/L],the estrogen level in high dose group[(27.39 ± 2.11) ng/L] was significantly reduced (P ＜ 0.01).Conclusion Ethephon has reproductive toxicity,and can cause the serum level of testosterone and estradiol decreased,resulting in spermatogenic cell apoptosis index increased and the spermatogenic capability decreased.
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Objective To establish the RP-HPLC method for the determination of myricetrin and quercitroside in Polygonum aviculare L.Methods The Agilent LC-C18 (250 mm×4.6 mm,5 μm) column was used,the mobile phase consisted of acetonitrile:0.1％ H3PO4(18 ∶ 82),the flow rate was 1.0 ml/min,the column temperature was 30℃ the detecting wavelength was at 256 nm.Results The calibration curve was linear within a range of 1.22～24.30 μg/ml and 0.77～15.40 μg/ml,the average recovery of this method was 98.9％,99.6％ and the RSD was 1.11％,1.09％,for the myricetrin and quercitroside respectively.Conclusion The method is simple,repeatable and accurate.It can be applied in quantitative determination of myricetrin and quercitroside in Polygonum aviculare L..
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Objective To establish an HPLC method for determining gallic acid in Chebulae fructus immaturus of different area.Methods The ZORBAX SB-C18 (250 mm×4.6 mm,5 μm) column was used,the mobile phase consisted of acetonitrile: 0.1％ H3PO4(29: 71),the flow rate was 1.0 ml/min,the column temperature was 30℃ the detecting wavelength was at 273 nm.Results Gallic acid was successfully separated within 20 min,the linear response range was 0.5611～5.611 μg.The average recovery was 99.9％,and RSD was 2.35％.Conclusion The method is simple,accurate and repeatable; it can provide evidence for further development and utilization of this crude drug.
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Objective To explore the increasing risk of herpes zoster and its possible mechanisms for hematologic disorders treated with arsenic trioxide (ATO). Methods The cases were divided into study group (with ATO) and control group (without ATO). The incidence rate of herpes zoster was compared between the two groups, and then the average cycles of chemotherapy were compared between the patients complicated with herpes zoster or not in study group. Results The rate of herpes zoster was significantly higher in study group than that in control group (χ2 =4.492, P =0.034). The rates of herpes zoster were 23.95 % (23/96) in study group and 7.89 % (3/38) in control groups. Patients in study group with herpes zoster had received 7.60 cycles and those without herpes zoster 7.72 cycles of chemotherapy on average (Z=0.976, P=0.296).Conclusion The risk of herpes zoster complication in hematologic disorders was increased after ATO treatment which probably activated varicella-zoster virus.
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Objective To evaluate the efficacy and safety of infliximab and traditional disease modifying antirheumatic drugs (DMARDs) in the treatment of ankylosing spondylitis (AS). Methods Sixty patients with definite AS were treated with infliximab 5 mg/kg infusion at 0, 2, 6, 12 weeks and were followed up for 12 weeks. The primary endpoint was proportion of ASAS 20 responders at week 12. The secondary endpoints were the proportion of ASAS 50, the change from baseline in Bath AS functional index (BASFI).The improvement of signs and symptoms of AS and physical function were evaluated. The statistical treatments were used t-test andA2 test. Results The proportion of ASAS 20 responders at 2, 6, 12 week was 70%, 83% and 93% respectively. The proportion of ASAS50 responders at patients at 2, 6, 12 week was 13%, 37% and 57% respectively. Results for other secondary efficacy endpoints showed that infliximab could provide substantial benefits to patients with AS by reducing clinical signs and symptoms and improving range of motion, physical function and quality of life. Ten percent of the subjects reported treatment- related adverse events. The most frequently occurred were upper respiratory tract infection, followed by gastrointestinal adverse events and infusion reaction. Most treatment-related adverse events were mild to moderate in severity and disappeared after drug withdrawal. Conclusion Infliximab has been demonstrated to be effective and is well tolerated in the treatment of AS.
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BACKGROUND: The prompt and efficient peripheral blood stem cell (PBSC) mobilization is necessary for repairing and regenerating injured tissues. Recombinant human granulocyte colony-stimulating factor (rhG-CSF) or its combination with a large dose of chemotherapy is usually utilized for PBSC mobilization, but the regimen requires a long time and a complex procedure. OBJECTIVE: To modify the common PBSC mobilization, and investigate mobilization effect of PBSC in KM mice with short-duration and high-dose rhG-CSF. DESIGN, TIME AND SETTING: A randomized controlled animal trial was carried out in the laboratory of Hematology, the Second Affiliated Hospital to Dalian Medical University from September 2006 to March 2007. MATERIALS: Twenty-four KM male mice of 8 months old were divided into 3 groups according to the administration: conventional regimen group, short-duration and high-dose group, and saline control group. METHODS: Mice in the conventional regimen group were injected subcutaneously with rhG-CSF (0.2 ?g one time, twice a day for 6 days). Mice in the short-duration and high-dose group were injected with equal volume of normal saline at first 4 days, and then with rhG-CSF (2.5 ?g one time, twice a day for 2 days). Mice in the saline control group were injected subcutaneously with equal volume of normal saline for 6 days. MAIN OUTCOME MEASURES: Peripheral blood white blood cell (WBC) and colony-forming unit-granulocyte macrophage in KM mice were counted. RESULTS: WBC in the peripheral blood of KM mice increased rapidly in conventional regimen group and short-duration and high-dose group. No obvious change of WBC was observed in the control group. The number of colony-forming unit-granulocyte macrophage in conventional regimen group and short-duration and high-dose group was significantly elevated over 50 folds than control group (P 0.05). CONCLUSION: There is a good efficacy and feasibility in PBSC mobilization of KM mice with short-duration and large-dose rhG-CSF, and it is a successful amelioration of PBSC mobilization of mice peripheral blood.
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OBJECTIVE:To establish a HPLC method for the content determination of baicalin in Pneumonia I mixture.METHODS:The assaying was conducted on a ODS C 18 column with methol-0.06%H 3 PO 4 (43∶57)as mobile phase,the detection wavelength was278nm,the column temperature was25℃and the flow rate was1.0ml/min.RESULTS:The linear concentration range of baicalin was0.1875?g～1.5?g(r=0.9998)with average recovery rate at99.63%(RSD=1.00%,n=6).CONCLUSION:This method is simple,accurate and reproducible,and it can be used for the quality control of baicalin.