Incidence of ochratoxicosis in poultry in Egypt

In an intensive poultry farm, Monofia Governorate, severe outbreaks occurred in the flock. Blood samples were taken from 50 severely affected as well as from 50 apparently healthy chicks to study the biochemical alterations in serum of birds. Moreover, five diet samples consumed by the chicks were obtained for detection of rancidity or mouldness. Samples of kidneys, liver, gizzards, parts of the muscles and intestines were examined for detection of mould residues. Results obtained indicated high contamination of the diets with ochratoxin A, which resulted in severe alteration of blood constituents and high residues in the internal organs, which led to high mortalities and severe economic losses in the flock

Utility of tetrazolium blue for the spectrophotometric determination of azlocillin and ceftriaxone

A simple and sensitive spectrophotometric method was described for the assay of azlocillin and ceftriaxone in injections. The method was based on the reduction of tetrazolium blue in alkaline medium by the two drugs, giving a highly colored formazan measurable at 524 nm. The optimization of the different experimental conditions was described. The method proved to be accurate and reproducible as indicated by a relative st and ard deviation of less than 2%

Spectrophotometric determination of mequitazine and thiopentone sodium in dosage forms

A simple and sensitive spectrophotometric method was described for the determination of mequitazine and thiopentone sodium. The method was based on the formation of ferroin upon reaction of these drugs with iron [III]-o-phenanthroline reagent. The colored product was quantitated spectrophotometrically at 510 nm. Beer's law was obeyed over concentrations ranging from 0.2 to 0.8 mg% and 2 to 6 mg% for mequitazine and thiopentone sodium, respectively. The proposed method has been applied successfully to the determination of the investigated drugs in dosage forms

Spectrophotometric determination of mequitazine

Two simple and sensitive spectrophotometric assay procedures were described for the determination of mequitazine. The first was based on the reaction of the drug with aconitic anhydride to give a red colored product measurable at 547 nm. The second depended on the oxidation of the drug with sodium nitroprusside in the presence of concentrated HCl to give a violet color with a maximum absorbance at 569 nm. Optimization of the experimental conditions was described. The proposed methods were applied to the determination of mequitazine in tablet form

Spectrophotometric determination of amoxicillin and cefadroxil using 3-methyl-2bencothiazolone hydrazone hydrochloride

A spectrophotometric method was described for the determination of amoxycillin and cefadroxil. The method was based on the reaction of these drugs with 3-methyl-2-benzothiazolone hydrazone hydrochloride [MBTH] in the presence of cerric ammonium sulfate. The colored products were estimated spectrophotometrically at 530 nm and 511 nm for amoxycillin and cefadroxil, respectively. Beer's Law was valid over the concentration range 10-50 mug ml -1. The method was applied successfully to the analysis of dosage forms. Relative st and ard deviations for the assay of both drugs in commercial preparations were less than 1%

First derivative spectrophotometric determination of weakly UV absorbing terfenadine and bencyclane

Rapid first derivative procedure is described for the determination of terfenadine and bencyclane in pure samples and in dosage forms. The method is based on the reaction of these drugs as n-electron donors with picric acid as ceta-acceptor, to form stable charge-transfer [CT] complexes. The coloured products are quantified by measuring the first derivative [D[1]] peak amplitude at 430 nm and at 425nm for terfenadine and bencyclane, respectively, or the peak-trough amplitude at 360-325 nm for terfenadine. Beer's law is obeyed over the concetration range 4-20 micro g ml[-1] of the investigated drugs. The proposed method is simple and sensitive. Reproducible, accurate and reliable results were obtained. The results were statistically compared with those of the standard methods. Terfenadine, alpha-[4-[1,1-dimethylethyl] phenyl]-4- [hydroxydiphenylmethyl] -1- piperidinebutanol, is a selective histamine H[1]-receptor antagonist used to relieve the symptoms of allergy. Bencyclane, N,N-dimethyl -3- [[1-[phenylmethyl]-cycloheptyl] oxy]-1-propanamine, is vasodilator which has been given in the treatment of peripheral and cerebral vascular disorders. Terfenadine and bencyclane are weakly UV absorbing compounds. Their [E 1%] ranged from 14.3-14.5 [1]. Therefore, the application of simple spectrophotometric methods is subjected to serious positive system error. To overcome this problem, many techniques have been reported [2-8]. Picric acid has been proved to be valuable reagent for the determination of some basic compounds [9,10]. Terfenadine and bencyclane have been also determined spectrophotometrically [11-15], coulometrically [16], GLC [17-20], HPLC [21,22] and radioimmunoassay [23.25]. This paper describes the application of the seta-acceptor picric acid [26] to the first derivative spectrophotometric determination of terfenadine and bencyclane as n-electron donors, in pure samples and in dosage forms