ABSTRACT
Teucrium polium [lamiaceae] is a natural source containing numerous antioxidant compounds and T. polium had advised for treatment of wide range of diseases including gout, inflammation and diabetes. In this Study, inhibitory effect of extracts, the most important antioxidant contents of T. polium and correlation between them had determined. Flowering branches of T. polium were collected from nine regions in north-eastern provinces of Iran and dried and powdered afterwards. The inhibitory effects of the plant extract on xanthine oxidase were assayed in vitro. Total Phenol and anthocyanin and soluble sugar content of each extract were matured. Then, their correlations with the inhibitory effects on xanthine oxidase were also determined. All these measurements were repeated three times and variance analysis was used for comparing means. All extract in different habitat exhibited a good inhibition effects on xanthine oxidase activity, the concentration of 0.3 mg/ml of samples were inhibited the enzyme from 11.44 to 91.45%. The highest inhibitory effect on xanthine oxidase was found in Ramian by 91.45%. Anthocyanin content in Golestan's samples [Tilabad] was remarkably more than other samples, 4.26 mg/g DW. Razari khorasan's samples [Garmab] had the highest of total phenol and soluble sugar contents, 28.11 and 6.84 mg/g DW respectively. These results suggest that T. polium extract in different regions is a rich source of antioxidant and has inhibitors effect on xanthine oxidase. Golestan samples had the highest inhibitory effect on the xanthine oxidase activity and are recommended for pharmacological studies
ABSTRACT
It is well known that the modification of oligosaccharide moieties of the cell surface glycoproteins modulates the adhesion and metastatic potential of several cancerous cell lines. Based on this knowledge, the anti-metastatic property of Daphne mucronata crude extract and one of its newly characterized active component, Gnidilatimonoein, were evaluated in wehi-164 cells by measuring their adhesion to fibronectin coated plates, relative to Castanospermine and Tunicamycin treated cells.Twenty four hours after treatment of the cells with the plant crude extract [equivalent to 0.54 mg of the plant leaves powder/ml], Gnidilatimonoein [0.94 nM], Castanospermine [2.6 micro M] and/or Tunicamycin [2.4 micro M], their attachment to fibronectin-coated wells were depressed, by 24%, 30%, 26% and 58%, respectively. This data may classify the new anticancer compound, Gnidilatimonoein, as a strong glycosylation inhibitor
Subject(s)
Plants, Medicinal , Tunicamycin , Glycosylation , Neoplasm Metastasis , Cell AdhesionABSTRACT
The adhesive interaction between tumor cells and the host cells or the extracellular matrix plays a crucial role in tumor metastasis. To evaluate the mediation of cell adhesion by Daphne mucronata, an anti-cancer medicinal plant in Iranian folk medicine, the adhesion of thrombin activated human platelets to the cultured monocytes or HL-60 cells was investigated under the effect of the plant extract [0.54 mg/ml] or one of its purified components [gnidilatimonoein, 0.94 micro M]. Treatment of the platelets with the plant extract or the active component, for various time intervals, followed by their activation by thrombin resulted in 80-90% reduction in the number of monocytes with more than 10 attached platelets. Similarly, under almost all identical conditions, the adhesion of the activated platelets to HL-60 cells was decreased by 90%. The adhesion of thrombin activated human platelets to the plant treated HL-60 cells was also reduced significantly [by 95%]. These data clearly indicate that Daphne mucronata is capable of mediating tumor metastasis through effecting cell adhesion properties of the cells