effect of oral sultamicillin on subgingival microflora

The dark field microscopy was used to study the effect of oral sultamicillin on subgingival microflora. Twelve patients were subjected to scaling for one side and the other side left without scaling. Bacterial samples were obtained from both sides before sultamicillin therapy at zero time and at 2 and 4 weeks after sultamicillin therapy. The bacterial samples, were investigated using dark field microscopy for identification and counting. There was a significant eradication in all bacterial cell count for both sides compared with the initial count. The most significant eradication was in motile rods followed by spirochetes. Microbiologically, sultamicillin therapy can affect the subgingival microflora quantitatively and qualitatively

Therapeutic effect of tinidazole in periodontal disease I- clinical evaluation

Twenty-four patients participated in the study. Every patient received scaling and root planing for one side and the other side was left without scaling to serve as control. All patients were given 1 g of tinidazole for 2 days. The gingival index, plaque index, periodontal index and the periodontal pocket depth were recorded before scaling and at 2, 6, 8 weeks. There was an improvement in the periodontal health status, which may suggest the use of tinidazole as valuable adjunct in the periodontal therapy

Lymphocyte blastogenic response to collagen in periodontal disease is predominantly a B-cell response and dependent upon adherent cells

Peripheral blood lymphocytes patients with periodontal disease undergo blastogenesis with human collagen type I. The cellular characteristics of this response were examined by fractionation of the cell populations and assay of thymidine uptake of responding cells. They were separated on nylon wool columns and monoclonal antibodies [OKT3 and anti-Lew M2] were used to further characterize the cell separation method. B-lymphocytes [OKT3 -] responded at higher levels to collagen than T-lymphocytes [OKT3 +]. Depletion of polystyrene adherent cells reduced the response to a greater extent. Reconstitution with approximately 10% adherent cells gave the maximal lymphoblastogenic response to collagen

Human T-Lymphocyte subpopulations in periapical lesions

Both B-lymphocyte- and T-lymphocyte-mediated immunologic reactions have been implicated in the pathogenesis of human periapical lesions. Thirteen periapical lesions were obtained during periapical surgery, frozen and serially sectioned. Monoclonal antibodies and indirect immunofluorescence techniques were used to compare the distribution of lymphocyte subpopulation in the periapical lesions. The proportions of total T-cells [T11], inducer/helper T-cells [T4+] and cytotoxic suppressor T cells [T8] were determined. No significant statistical difference was found between the number of T helper cells and that of the T cytotoxic cells. The presence of T-cells in periapical lesions indicated that cell-mediated reactions participate in the pathogenesis of these lesions

Therapeutic effects of tinidazole in periodontal disease

Eight patients were participated in this study. Every patient received scaling for one side and the other side left without scaling. Bacterial samples were obtained from both sides before tinidazole therapy [at zero time] and at 2 and 4 weeks after tinidazole therapy. Dark field microscopy was used for bacterial identification and counting. There was a reduction in the total cell count. The counts of spirochetes, rods [motile and non-motile] showed decrease than at the initial counts. The cocci showed a concomitant increase in week 2 and 4 than at week 0. Microbiologically, tinidazole therapy can affect the subgingival microflora quantitatively and qualitatively

Histopathologic investigation of minor salivary glands in iron deficiency anaemia

Labial minor salivary glands were investigated in 17 patients of both sexes suffering from iron deficiency anaemia; and 17 age and sex matched healthy subjects. The most pronounced changes were demonstrated in iron deficiency anaemia; where there was a significant decrease in the size of the maturation compartment cells which was due to reduced cyto-plasmic volume. Increased nucleo-cytoplasmic ratio was also observed. Tubular metaplasia with progressive acinar atrophy was also noted and induced reduction of salivary function. The observable qualitative changes in minor salivary glands could be-postulated as a warning signal for premalignancy in patients with iron deficiency anaemia. The histologic data of minor salivary gland involvement could be used as an aid for diagnosis and prognosis in iron deficient patients