Correlaton between estrogen receptors and histo-pathological parameters in human breast cancer

A monoclonal antibodies immunoassay technique was used to demonstrate oestrogen receptors in 50 in freshly excised human breast tumours. 48 cases were infiltrating duct carcinoma and 2 cases were infiltrating lobular carcinoma. The relationship between oestrogen receptors, histopathological type, histopathologicalIy and nuclear grades, fibrosis, elastosis and necrosis was evaluated. Oestrogen receptors positivity was demonstrated in 44 out of 50 cases. A significant positive correlation was found between histopathological and nuclear grades and oestrogen receptors content, well differentiated tumours rarely lacked oestrogen receptors. Tumours with marked fibrosis showed less oestrogen receptors content than tumours with mild fibrosis, Elastotic tumours were more oestrogen receptors positive than non elastotic tumours. Those with marked elastosis showed higher oestrogen receptors content than tumours with moderate and mild elastosis. A significant correlation was found between oestrogen receptors content and necrosis, tumours without necrosis showed higher oestrogen content than those with necrosis. The previous results may explain significant higher 2 year recurrent survival in cases with well differentiated tumours, little fibrosis and absent necrosis found in the present study

Immunohistochemical study of epithelial cell markers in inverted papilloma of the nose and paranasal sinuses

The expression of keratin, carcino embryonic antigen [C.E.A] and epithelial membrane antigen [E.M.A] were studied in the epithelial linings of 9 cases of inverted papilloma of the nose and para-nasal sinuses, using an indirect immunoperoxidase method on formaline fixed paraffin embedded sections 8 cases were positive with polyclonal antibodies of keratin specificity. The pattern of expression of C.E.A. and E.M.A. differed it was suprabasal in 7 cases with C.E.A. and basal in 5 cases with [E.M.A.] although these differences may not be of diagnostic significance, the constant expression of keratin may provide a useful marker of inverted papilloma

Histopathological and histochemical, effects of snake venom and antivenom: an experimental study

The work is an attempt towards a better and clear understanding of the effect of snake venom [Ceriastes cerastes] on the different tissue of the experimental of the results occurring after the use of antivenom in the treatment of envenomation was done.The study includes the following groups : Group I: To demonstrate the effect of single different doses [0.5, 1.15 and 2 ug/gm body weight] of Cerastes venom The different organs taken [Liver, Kidneys, lungs and heart] showed marked histopathological and histochemical [alkaline phosphatase] changes which their degree being proportional according to the dose given. On in the other hand, the brain was the least sensitive of all the organs examined.Group II : To demonstrate the effect of repeated small doses [0.5 ug/gm, body weight] of Cerastes cerastes venom every other day for six doses on the different tissues of guineapigs. The same changes with group I were noticed but to a lesser extent. Group III: To demonstrate the effect of polyvalent antivenom after the venom.The toxic-induced changes were markedly reduced. It is to be concluded from this study that single different doses of Cerastes cerastes venom have both histopathological and histochemical effects on different tissue and the degree of these effects being proportional to the dose given. Repeated small doses have a lesser effect. It is recommended that the appropriate antivenom should be given to prevent or reduce the damaging effect of the venom

Pathological changes in products of conception of aborters with deficient immunologic block factor [s]

The study was performed on 20 aborters 13 of which were seronegative in the blocking factor. The pathological changes especially vasculitis, cellular infiliterate of lymphocytes, histiocytes as well as fibrosis of chorionic villi were more frequent and more marked in these seronegative cases than in seropostive ones. The possible role of absence of blocking factor in production of this changes and hence termination of pregnancy was discussed

Histopathology of cervix after injectable contraceptives

This study included 40 women aged between 25- 40 years. 20 of them were injected I.M. with Depoprovera 150mg every 3 months for 5 injections [15 months], and the other 20 were injected with Noresterat 200mg every 2 months for 7 injections [14 months]. Punch biopsy was taken from the cervix before and after the injections to show the histopathology of the cervix. With Depoprovera 3 cases of mild dysplasia, 2 cases of microglandular hyperplasia, and 2 cases of squamous metaplasia. In Noresterat users, one case of mild dysplasia, one case of microglandular hyperplasia, and 2 cases of squamous metaplasia