ABSTRACT
Objective@#The appropriate function of the hypothalamic-pituitary-gonadal axis is essential for maintaining proper reproductive function. In female mammals, the hypothalamic-pituitary-gonadal axis regulates reproductive changes that take place in the estrus cycle and are necessary for successful reproduction. This study was conducted to investigate the effect of thymectomy on the estrus cycle in neonatally thymectomized guinea pigs. @*Methods@#In this study, 12 female guinea pigs, six thymectomized and six sham-operated, were studied. The effects of neonatal thymectomy at 5–7 days of age on parameters of the reproductive axis were examined in female guinea pigs. Gonadotropin and 17β-estradiol levels were assessed at regular intervals (days 0, 3, 6, 9, 12, and 15) of the estrus cycle, and the time of vaginal opening in the thymectomized and shamoperated guinea pigs was determined. @*Results@#Significant reductions in gonadotropins and 17β-estradiol levels during estrus cycle were found in neonatally thymectomized female guinea pigs compared to sham-operated guinea pigs. @*Conclusion@#The results of this study underscore the importance of the thymus in the neonatal period for normal female reproductive function.
ABSTRACT
Introduction: The presence of the thymus gland during early life is essential for the normal development ofimmune system and as well as proper maturation of the hypothalamic – pituitary- gonadal axis. This study wasto investigate the influence of thymus gland on male reproductive function.Aim: The aim of this study to investigate the effect of thymectomy on Morphometric changes of gonads inneonatal, prepubertal and pubertal thymectamized male guinea pigs.Material and Methods: In this study, a total of thirty male guinea pigs, thymectamized (n-6) and sham-operated(n-4) were studied in each group. The comparison of diameter of seminiferous tubules, luminal diameter andepithelial thickness in the thymectamized and sham operated male guinea pigs.Results: No Significant changes in the morphometry of seminiferous tubules of testis in thymectamized maleguinea pigs compared to the sham-operated guinea pigs in all age groups. These results suggest that thymectomyhad no detrimental effects on reproductive system in males at any intervals.
ABSTRACT
The Development of an analytical method for the determination of 24 multiclass pesticides in mango at the ≤10 ng g-1 level. The method involves extraction of 10 g of homogenized mango samples (2 g of Sodium chloride- +8g of Magnesium sulfate) with 10 mL of Acetonitrile; Clean up by Dispersive solid phase extraction with a combination of primary secondary amine (PSA), graphitized carbon black (GCB) and anhydrous Magnesium sulfate and final estimation by LC-MS/MS with multiple reaction monitoring (MRM) mode. The mean recoveries were in the range 80-120%. The method quantifies over a linear dynamic range of 10-100 μg/kg. The methodology has been proven to be highly efficient and robust and thus the method is suitable for monitoring the Maximum Residue Limits (MRL) compliance of a wide range of pesticides combinations.