ABSTRACT
Objective To investigate the expression of programmed death ligand-1(PD-L1) in glioma and its influence to intratumor infiltrating T lymphocyte apoptosis.Methods PD-L1 in 18 cases of normal brain tissues and 80 cases of glioma tissues treated in this hospital during 2007 to 2011 was determined by immunohistochemistry staining,meanwhile the PD-L1 expression in glioma infiltrating T lymphocyte was determined.The correlation analysis was performed.The apoptosis of intratumor infiltrating lymphocytes was examined by TUNEL assay;the peripheral blood activated T lymphocyte apoptosis promoted by PD-L1 was analyzed by flow cytometry.Results No expression of PD-L1 in normal brain tissues was observed,but the positive expression rate in glioma was 42.50 ％ (34/80),which was correlated with pathological grade,necrosis and prognosis(P＜0.05).PD-1 was expressed in part of tumor infiltrating T lymphocytes and glioma tissues,moreover the lymphocyte apoptosis expression in the cases of PD-L1 positive glioma was significantly increased compared with that in the cases of PD-L1 negative glioma.The apoptosis rate of activated T lymphocytes was 42.55 ％ respectively,which was significantly higher than that in the blank group;however,adding anti-PD-1 for blocking the combining of PD-L1 with T lymphocyte,the lymphocytes apoptotic rate in the PD-L1+ anti-PD-1 group was dropped to 26.80％.Conclusion PD-L1has aberrantly high expression in human glioma and is combined with PD-1 to promote the apoptosis of lymphocyte.
ABSTRACT
Objective To explore the effects of poly(ADP-ribose) glycohydrolase (PARG) gene silencing on colon carcinoma lovo cells matrix adhesion,migration and invasion.Methods Lentivirus PARG-shRNA was transfected into colon carcinoma lovo cells and the lovo cell strain with PARG gene silencing perpetually was selected.Western blot was used to test the expression of PARG,poly(ADP-ribose) polymerase(PARP) and NF-κB.The lovo cell matrix adhesion,migration and invasion potencies were observed by cell matrix adhesion,migration and invasion assay.Results The expression of PARP and NF-κB in experiment groups was weaker than that in the control groups (P＜0.05).PARG gene silencing of lovo cells decreased the cell matrix adhesion,migration and invasion potencies.The inhibitory rates of lovo cell matrix adhesion,migration and invasion were 25.22%,38.71%,35.29% respectively.Conclusion PARG gene silencing of lovo cells could inhibite cell matrix adhesion,migration and invasion potencies,which may relate to that PARG gene silencing and decreasing the activity of PARP and NF-κB.PARG probably plays an important role in the infiltration and metastasis of tumor.
ABSTRACT
Objective:Poly(ADP-ribose) glycohydrolase(PARG) plays an important role in the inflammation via regulating Poly(ADP-ribose) polymerase(PARP) and AKT phosphorylation,but it is not clear that how it in tumour.The current study was desiged to study the relationship between PARG,PARP,NF-?B and AKT phosphorylation in colorectal carcinoma cell lines LOVO.Methods:Western blot was used to test the expression of PARG,PARP,NF-?B,AKT and PI-AKT473.Gallotannin(GLTN) was served as PARG inhibitor.Results:The expression of PARG,PARP and NF-?B in gallotannin-treated LOVO was weaker than that in the control groups(gallotannin-untreated groups)(P=0.0068,P