ABSTRACT
Los flavivirus transmitidos por mosquitos son una amenaza actual y emergente en todo el mundo. Dentro de este género, el virus Encefalitis San Luis (VESL) causa una forma severa de enfermedad neuroinvasiva donde la respuesta inmune es un componente crucial de la defensa del huésped. En este trabajo se investigó la interacción entre VESL y células de la inmunidad innata, en un modelo de infección in vitro de monocitos humanos (células U937) con cepas de distinta virulencia y condiciones epidemiológicas de aislamiento (CbaAr-4005 y 78V-6507). Se evaluó la capacidad de infectar y replicar del virus, como también el efecto citopático y la cinética de viabilidad de monocitos durante la infección. Los resultados demostraron la susceptibilidad de los monocitos a la infección, replicación y muerte por ambas cepas virales. Sin embargo, se hallaron diferencias significativas entre ellas. La cepa epidémica y de mayor virulencia CbaAr-4005 registró una tasa de infección y replicación superior a la de la cepa endémica y de menor virulencia 78V-6507. Se comprobó también que el VESL indujo la muerte de monocitos humanos, dependiendo del tiempo post-infección (pi) y de la cepa. Así, CbaAr-4005 provocó a partir del día 3 pi el doble de mortalidad celular que 78V-6507. Además, en los monocitos infectados se observaron alteraciones de parámetros morfológicos que podrían relacionarse con el tipo de mecanismo de muerte celular asociado a la infección por VESL.
Mosquitoes borne Flavivirus infections are an actual and emergent worldwide threat to human health. Within this genus, Saint Louis Encephalitis Virus (SLEV) causes a severe neuroinvasive disease where immune response is crucial for host survival. In this study the interaction between SLEV and innate immune cells was evaluated. An in vitro infection model with human monocytes (U937 cells) and strains with variations in virulence and isolation conditions (CbaAr-4005 and 78V-6507) were used. Infection capacity, replication capacity, cytopathic effect and monocyte viability kinetics were measured. The results showed susceptibility to infection and replication to both strains. However, significant differences were found among them. CbaAr-4005, the epidemic and more virulent strain, showed higher infection and replication ratios compared to 78V-6507. SLEV infection that induces cell death of human monocytes was also found in a post-infection time and in a strain dependent manner. Since day 3 post-infection, twice the mortality in CbaAr-4005 infected cells was observed. Furthermore, infected monocytes showed alterations in morphologic parameters that could be related with apoptosis mechanisms associated to SLEV infections.
Os Flavivírus transmitidos por mosquitos são uma ameaça atual e emergente no mundo todo. Nesse gênero, o vírus Encefalite Saint Louis (VESL) causa uma forma grave de doença neuroinvasiva onde a resposta imune é um componente crucial da defesa do hospedeiro. Neste trabalho nos investigamos a interação entre VESL e células de imunidade inata em um modelo de infecção in vitro de monócitos humanos (células U937) com estirpe de diferentes virulências e condições epidemiológicas de isolamento (CbaAr-4005 e 78V-6507). Foi avaliada a capacidade do vírus de infectar e replicar , assim como o efeito citopático e a viabilidade cinética dos monócitos durante a infecção. Os resultados demonstraram a suscetibilidade dos monócitos à infecção, replicação e morte por ambas as estirpes virais. No entanto, foram detectadas diferenças significativas entre eles. A estirpe epidémica e de maior virulenta CbaAr-4005 teve uma maior taxa de infecção e replicação do que a estirpe endémica e menos virulenta 78V-6507. Foi comprovado também que o VESL induziu a morte de monócitos humanos, dependendo do tempo pós-infecção (pi) e da estirpe. Assim, a CbaAr-4005 causou a partir do dia 3 pi o dobro da mortalidade celular o que a 78V- 6507. Além disso, alterações nos parâmetros morfológicos foram observadas nos monócitos infectados que poderiam estar relacionadas ao tipo de mecanismo de morte celular associado à infecção pelo VESL.
Subject(s)
Humans , Male , Female , Virulence , Flavivirus Infections , U937 Cells , Encephalitis , Encephalitis Virus, St. Louis , Encephalitis Viruses/growth & development , Flavivirus , Patient Isolation , Viruses , In Vitro Techniques , Kinetics , Cells , Disease , Incidence , Causality , Mortality , Apoptosis , CulicidaeABSTRACT
Nuestro objetivo fue conocer la presencia de anticuerpos neutralizantes contra el virus de la encefalitis de San Luis (St. Louis encephalitis virus [SLEV]) y el virus del Nilo Occidental (West Nile virus [WNV]) en sueros de aves silvestres y domésticas de la Ciudad Autónoma de Buenos Aires, Argentina. Desde octubre del 2012 hasta abril del 2013 se colectaron 180 muestras que fueron procesadas por la técnica de microneutralización. El 7,2% de las aves muestreadas resultaron seropositivas para SLEV, mientras que no se detectaron aves seropositivas para WNV
Our goal was to determine the presence of neutralizing antibodies against St. Louis encephalitis virus (SLEV) and West Nile virus (WNV) in sera of wild and domestic birds from Buenos Aires City, Argentina. From October 2012 to April 2013, 180 samples were collected and processed by the microneutralization technique. A 7.2 % of the sampled birds were seropositive for SLEV, while no seropositive birds for WNV were detected
Subject(s)
Animals , West Nile virus/isolation & purification , Birds/microbiology , Encephalitis Virus, St. Louis/isolation & purification , Antibodies, Neutralizing/analysis , Serologic Tests/methodsABSTRACT
St. Louis encephalitis virus (SLEV) and West Nile virus (WNV) present ecological and antigenic similarities and are responsible for serious human diseases. In addition, WNV is a significant pathogen in terms of equine health. The purpose of our study was to analyse the seroprevalence of SLEV and WNV in equine sera collected in Santa Fe Province, Argentina. The seroprevalence determined using the plaque reduction neutralisation test was 12.2% for SLEV, 16.2% for WNV and 48.6% for a combination of both viruses. These results provide evidence of the co-circulation of SLEV and WNV in equines in Santa Fe.
Subject(s)
Animals , Encephalitis Virus, St. Louis/isolation & purification , Encephalitis, St. Louis/veterinary , Horse Diseases/virology , Horses/virology , West Nile Fever/veterinary , West Nile virus/isolation & purification , Argentina/epidemiology , Encephalitis Virus, St. Louis/immunology , Encephalitis, St. Louis/diagnosis , Encephalitis, St. Louis/epidemiology , Encephalitis, St. Louis/virology , Horse Diseases/diagnosis , Horse Diseases/epidemiology , Seroepidemiologic Studies , West Nile Fever/diagnosis , West Nile Fever/epidemiology , West Nile Fever/virology , West Nile virus/immunologyABSTRACT
Rio Negro virus (RNV) (Venezuelan equine encephalitis subtype VI) circulates only in Argentina; in northern provinces, isolates have been obtained from mosquitoes and rodents since 1980 and have been associated with acute febrile illness in humans. However, no studies of RNV have been performed in the central area of the country. We carried out molecular and serological detection of RNV in Córdoba, a province of the central part of the country, in mosquitoes and humans, respectively. One mosquito pool tested positive for alphavirus RNA by reverse transcriptase-nested polymerase chain reaction (RT-nested PCR). Subsequent sequencing determined that this alphavirus grouped with RNV. Serological studies detected antibodies to RNV in one human serum sample, which was obtained during the same period that RNV was detected using the aforementioned molecular methods. This is the first report of RNV circulation in the central area of Argentina, indicating an expansion of its original distribution. These results highlight the importance of strengthening surveillance procedures in endemic areas, as well as in new regions where RNV may emerge.
Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Animals , Child , Child, Preschool , Humans , Infant , Infant, Newborn , Middle Aged , Young Adult , Culicidae/virology , Encephalitis Virus, Venezuelan Equine/genetics , Encephalomyelitis, Venezuelan Equine/virology , Insect Vectors/virology , Antibodies, Viral/blood , Argentina/epidemiology , Encephalitis Virus, Venezuelan Equine/immunology , Encephalitis Virus, Venezuelan Equine/isolation & purification , Encephalomyelitis, Venezuelan Equine/diagnosis , Encephalomyelitis, Venezuelan Equine/epidemiology , Polymerase Chain Reaction , Reverse Transcriptase Polymerase Chain Reaction , RNA, Viral/analysisABSTRACT
Una virus (UNAV), Togaviridae family, is widely distributed in South America, where infections have been detected in mosquitoes and vertebrate hosts (humans, birds and horses). We analyzed human sera from Córdoba inhabitants aged 44 to 89 years and using a neutralization test, we found a prevalence of UNAV antibodies of 3.8 percent (3/79). The low titers detected suggest past infections probably acquired in rural areas of the Province of Córdoba (central Argentina). None sera were found positive for MAYV neutralizing antibodies. This is the first report of human infections by UNAV in Argentina
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Antibodies, Viral , Togaviridae , Togaviridae Infections , Aged, 80 and over , Argentina , Neutralization Tests , Seroepidemiologic StudiesABSTRACT
To determine hepatitis C virus (HCV) genotypes circulating in the central region of Argentina, 96consecutive anti-HCV positive subjects were studied. The presence of HCV RNA was detected in 60samples by RT-nested PCR of the 5Æ noncoding region (5Æ NCR). Genotyping was performed by restriction fragmentlength polymorphism analysis of 5Æ NCR region combined with PCR using type-specific primers of the core region.The groups of individuals in this study included hemophilia and hemodialysis patients, injecting drug users, screened blood donors, and patients with acute or chronic liver disease, all from Córdoba, Argentina. Overall, genotype 2 was the most prevalent (55.0%), followed by genotypes 1 (38.3 %), and 3 (5.0%). Within genotype 1, subtype 1b was the most prevalent. An unexpected high prevalence of genotype 2 (61.9%) was found among patients with acute or chronic HCV infection (without known risk factors). These figures differ from other cohorts from East-Argentina where genotype 1 has been found as the most prevalent. This indicates that regional differences of genotype distribution might exist between Central and East Argentina.
Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Hepacivirus , Hepatitis C , Hepatitis C Antibodies , Argentina , DNA, Viral , Genotype , Hepacivirus , Hepatitis C , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , PrevalenceABSTRACT
A serological screening was performed in 615 individuals aged 0-87 years, living in the city of Cordoba, Argentina to study the relationship between antibody prevalence for the SLE virus and age. A 13.98 percent prevalence of neutralizing antibodies was obtained and its relation to age was significantly high (p = 0.045). The highest seroprevalence was noted on individuals over 60 years old (>20 percent), whereas no subject under 10 was seropositive for this virus. Our results confirm that the agent is endemic in this area and neurological pathology studies should be performed on those individuals aged 60 since they represent the most susceptible group to SLE virus
Subject(s)
Humans , Male , Female , Infant, Newborn , Infant , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Antibodies, Viral , Encephalitis Virus, St. Louis , Encephalitis, St. Louis , Age Factors , Aged, 80 and over , Argentina , Logistic Models , Mass Screening , Neutralization Tests , Prevalence , Seroepidemiologic StudiesABSTRACT
An in house indirect immmunofluorescence assay ( IFA ) in relation to neutralization (NT) reference test, was assessed as a fast and cheap method to carry out serological surveys for St. Louis Encephalitis virus (SLE). Sera obtained from 213 blood donors were analyzed by both tests. The prevalence of seropositivity obtained with IFA was lower than (30.98 percent) that observed on NT (41.78 percent). The relative specificity rate of IFA was 96.77 percent whereas its relative sensitivity rate was 69.66 percent. Kappa index showed a good correlation between both tests. The results indicate that neutralization assay is still the serological test with the highest sensitivity and specificity relative rates for detecting antibodies against SLE virus. Nevertheless, the IFA could be useful as an alternative test in order to learn the circulation of the Flavivirus genus in a certain area
Subject(s)
Humans , Antibodies, Viral , Encephalitis Virus, St. Louis , Antibodies, Viral , Fluorescent Antibody Technique, Indirect , Neutralization Tests , Sensitivity and SpecificityABSTRACT
El objetivo del presente trabajo fue conocer la prevalencia de anticuerpos para el virus encefalitis San Luis (ESL) en sueros humanos del Laboratorio de Vigilancia Epidemiológica del departamento Formosa (provincia de Formosa, Argentina), extraídos en los años 1995 y 1997, se emplearon las pruebas de inhibición de la hemaglutinación (IH) y de neutralización (NT). El tamizaje realizado mediante la prueba de NT mostró una prevalencia del 21 por ciento (60/284) y 32 por ciento (50/157) en las muestras obtenidas en los años 1995 y 1997 respectivamente. El 14 por ciento de los sueros del año 1995 presentaron títulos de anticuerpos neutralizantes (NT) bajos (1/20 y 1/40) mientras que en el año 1997 el 19 por ciento de los sueros tenían títulos de anticuerpos NT iguales o mayores a 1/80. En la titulación simultánea de anticuerpos por NT e IH se observaron sueros con títulos bajos de anticuerpos NT (1/20-1/40) y negativos en IH, mientras que otros poseían títulos altos por ambas pruebas. Esta relación entre los títulos de anticuerpos indica la presencia de infecciones pasadas y recientes y la circulación continua de este virus. Por otra parte la prevalencia de anticuerpos NT en la población estudiada se incrementó significativamente en 2 años (p < 0.0075), lo que confirma la endemicidad de este agente en la zona y muestra la necesidad de realizar estudios de las enfermedades febriles de etiología viral no confirmadas, a fin de conocer su importancia como patógeno humano en nuestro país.