Postural balance on basketball injuries / Equilíbrio postural sobre as lesões no basquetebol / Equilibrio postural en las lesiones de baloncesto

ABSTRACT Introduction: Contemporary basketball has become more competitive and aggressive in the competition process, increasing the corresponding sport's risks. Objective: Analyze the injury risks arising from basketball and study the effects that postural balance training has on them. Methods: A controlled experiment was conducted, in which the experimental group used balance training and the control group used general physical training. Each training lasted one hour, twice a week, and the experimental period was nine weeks. Results: The total FMS scores of the two groups were improved before and after sports training, and the FMS scores of the control group were raised from 14.71 to 15.15, showing no significant difference (P>0.05). The total FMS score in the experimental group increased from 14.38 to 17.69, much higher than the experimental group. It was found that there was a very significant difference. Conclusion: The method proposed in this paper can effectively increase the postural balance ability of athletes, thus reducing the risk of sports injuries in the development of sports. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMO Introdução: O basquetebol contemporâneo tornou-se mais competitivo e agressivo no processo de competição, aumentando também os riscos esportivos correspondentes. Objetivo: Analisar os riscos de lesão decorrentes do basquetebol e estudar os efeitos que o treinamento de equilíbrio postural tem sobre eles. Métodos: Foi realizado um experimento controlado, no qual o grupo experimental empregou treinamento de equilíbrio e o grupo de controle adotou treinamento físico geral. Cada treinamento durou uma hora, duas vezes por semana, e o período experimental foi de 9 semanas. Resultados: As pontuações totais de FMS dos dois grupos foram aperfeiçoadas antes e depois do treinamento esportivo, e as pontuações de FMS do grupo controle foram elevadas de 14.71 para 15.15, mostrando que não houve diferença significativa (P>0.05). A pontuação total do FMS no grupo experimental aumentou de 14.38 para 17.69, muito superior à do grupo experimental. Constatou-se que houve uma diferença muito significativa. Conclusão: O método proposto neste trabalho pode aumentar efetivamente a capacidade de equilíbrio postural dos atletas, reduzindo assim o risco de lesões esportivas no desenvolvimento do esporte. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: El baloncesto contemporáneo se ha vuelto más competitivo y agresivo en el proceso de competición, aumentando también los correspondientes riesgos deportivos. Objetivo: Analizar los riesgos de lesiones derivados del baloncesto y estudiar los efectos que el entrenamiento del equilibrio postural tiene sobre ellos. Métodos: Se llevó a cabo un experimento controlado, en el que el grupo experimental empleó el entrenamiento del equilibrio y el grupo de control adoptó el entrenamiento físico general. Cada entrenamiento duró una hora, dos veces por semana, y el periodo experimental fue de 9 semanas. Resultados: Las puntuaciones totales de FMS de los dos grupos mejoraron antes y después del entrenamiento deportivo, y las puntuaciones de FMS del grupo de control aumentaron de 14,71 a 15,15, lo que demuestra que no hubo diferencias significativas (P>0,05). La puntuación total de FMS en el grupo experimental aumentó de 14,38 a 17,69, muy superior a la del grupo experimental. Se comprobó que había una diferencia muy significativa. Conclusión: El método propuesto en este trabajo puede aumentar eficazmente la capacidad de equilibrio postural de los atletas, reduciendo así el riesgo de lesiones deportivas en el desarrollo del deporte. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

miR-142-3p alleviates house dust mite induced airway inflammation among children / 中国学校卫生

Objective@#To investigate the role of miR-142-3p in alleviation of house dust mite induced allergic airway inflammation among children, so as to provide insights into unraveling the pathogenesis of allergic airway inflammation.@*Methods@#Serum samples were collected from 15 patients with house dust mite induced allergic asthma and 15 healthy children in Jiangnan University Medical Center from September to November 2022, and serum miR-142-3p expression was quantified using a fluorescent quantitative real time PCR (qPCR) assay. The levels of interleukin 6 (IL 6) and tumor necrosis factor α (TNF α) were measured in the cell culture supernatant using enzyme linked immunosorbent assay (ELISA), and the expression of high mobility group box 1 (HMGB1) was detected at transcriptional and translational lvels using qPCR and Western blotting assays. The negative regulation of the HMGB1 gene by miR 142 3p was identified using a dual luciferase gene reporter assay, and the expression of downstream regulatory proteins was determined in human normal lung epithelial cells (BEAS 2B) cells transfected with miR 142 3p using Western blotting. In addition, female C57BL/6 mice at ages of 6-8 weeks were randomly assigned to the phosphate buffer saline (PBS) group, house dust mite sensitized airway inflammation group and house dust mite sensitized airway inflammation + miR 142 3p intervention group. Mouse airway inflammation was evaluated using hematoxylin eosin staining, and the expression of inflammatory cells and inflammatory cytokines were detected in mouse bronchoalveolar lavage fluid (BALF) using Giemsa staining and ELISA.@*Results@#Lower serum miR-142-3p expression was quantified among children with house dust mite induced allergic asthma than among healthy controls (1.33±0.21 vs. 4.74±0.62, t=5.22, P <0.05). Stimulation with dermatophagoides farinae extract (DFE) resulted in a reduction in miR-142-3p expression in BEAS-2B cells (0.82±0.25), while transfection with miR-142-3p mimics resulted in a rise in miR-142-3p expression in BEAS-2B cells (0.55±0.14)( t=3.31, 3.94, P <0.05). Pre treatment with miR-142-3p reduced the expression of IL 6(2.25±0.46)and TNF α(6.58±1.95) ( t=4.86, 3.38, P <0.05) in BEAS 2B cells stimulated with DFE, and treatment with miR-142-3p mimics resulted in a reduction in TLR4 and NF-κB expression in BEAS-2B cells via negative regulation of the HMGB1 expression. In addition, treatment with miR-142-3p was found to alleviate inflammatory cell infiltration in lung tissues of house dust mite sensitized mice, and results in a reduction in interleukin 4 (IL-4)[(107.60±10.43)pg/mL], interleukin 5 (IL 5)[(95.78±13.14)pg/mL] and HMGB1[(2.52±0.87)pg/mL] expression in BALF ( t=10.32, 7.29, 2.90, P <0.05).@*Conclusion@#miR-142-3p alleviates house dust mite induced allergic airway inflammation among children via negative regulation of the HMGB1/TLR4/NF-κB pathway.

Coverage of enterovirus 71 vaccine among children born between 2018 and 2021 in Ningbo City / 预防医学

Objective@# To investigate the coverage of enterovirus 71 (EV71) vaccine among children born between 2018 and 2021 in Ningbo City, Zhejiang Province, so as to provide insights into formulating EV71 vaccination strategy.@*Methods@#Data pertaining to demography and EV71 vaccination among children born between 2018 and 2021 in Ningbo City were obtained from the Zhejiang Immunization Information Management System, and the coverage of vaccination, full-dose vaccination and timely vaccination of EV71 vaccine were analyzed. @*Results@#Totally 416 176 children were born between 2018 and 2021, including 216 977 boys (52.14%) and 199 199 girls (47.86%). The coverage rates of vaccination, full-dose vaccination and timely vaccination was 61.50%, 58.70% and 32.53%, respectively. The coverage rates of vaccination, full-dose vaccination and timely vaccination among local children (71.43%, 70.04% and 40.24%) were higher than those among migrant children (54.05%, 50.18% and 26.73%; P<0.05). The coverage rates of vaccination, full-dose vaccination and timely vaccination among urban children (67.72%, 65.06% and 40.37%) were higher than those among rural children (54.11%, 51.14% and 23.21%; P<0.05). The coverage rates of vaccination were 54.98%, 61.24%, 65.10% and 66.72%, the coverage rates of full-dose vaccination were 52.16%, 58.72%, 62.44% and 63.39%, and the coverage rates of timely vaccination were 22.47%, 25.86%, 39.14% and 48.03% by the born year from 2018 to 2021, all showing increasing trends year by year (P<0.05). @*Conclusion@#The coverage of vaccination, full-dose vaccination and timely vaccination of EV71 vaccine appears a tendency towards a rise among the 2018-2021 birth cohorts in Ningbo City, while the coverage of timely vaccination need to be improved. Health education for EV71 vaccination should be enhanced for rural and migrant children.

Intention and influencing factors of medical staff in community diabetes management to recommend influenza vaccination / 预防医学

Objeetive@#To understand the intention and influencing factors of medical staff in community diabetes management to recommend influenza vaccination,so as to provide reference for promotion of influenza vaccination recommendation.@*Methods @#The Doctors, nurses and managers from 8 community health service centers（hospitals）in Jiangbei District of Ningbo,who provided diabetes management services,were recruited to collect their demographic features,job satisfaction and intention to recommend influenza vaccination. Logistic regression model was used to analyze the influencing factors for the intention to recommend. @*Results@#Totally 412 questionnaires were distributed and 399（96.84%）valid questionnaires were recovered. There were 178 general practitioners,161 nurses,20 managers and 40 other staffs,accounting for 44.61%,40.35%,5.01% and 10.03%, respectively. Among them,281 had the intention to recommend influenza vaccination,accounting for 70.43%. The results of multivariate logistic regression analysis showed that the medical staff who aged 30 to 39 years（OR=0.332,95%CI：0.118-0.932）,worked for more than 20 years（OR=0.136,95%CI：0.044-0.425）, concerned about medical disputes（OR=0.170,95%CI：0.074-0.392）and negative medical opinion（OR=0.336,95%CI：0.141-0.803）were less likely to recommend influenza vaccination;while the medical staff who were general practitioners（OR=3.664,95%CI：1.513-8.869）,managers（OR=14.695,95%CI：2.494-86.591）,and who were satisfied with professional training opportunities（OR=5.041,95%CI：1.686-15.072）,financial or moral rewards（OR=8.216,95%CI：3.213-21.011）,personal accomplishment（OR=3.955,95%CI：1.517-10.310）and interest in work（OR=6.669,95%CI：2.667-16.679）were more likely to recommend influenza vaccination.@*Conclusion@#Age,post,working age,medical dispute concern,negative medical opinion,professional training opportunities,financial or moral rewards,personal accomplishment and interest in work are associated with the intention to recommend influenza vaccination of the medical staff in community diabetes management.

Expression and biological significance of GABRE in colon cancer: An analysis based on data mining of Oncomine and TCGA databases / 中国肿瘤生物治疗杂志

@#[Abstract] Objective: To explore the expression and biological significance of GABRE gene in colon cancer by mining data in the Oncomine and TCGA databases. Methods: The expression of the GABRE gene in colon cancer tissues and its correlation with the prognosis of patients were analyzed using the Oncomine and TCGA databases. The upstream miRNA targeting GABRE gene was identified using TargetScan, starBase, mirDIP, and miRWalk, and its expression and relationship with prognosis of colon cancer were analyzed. Furthermore, the GABRE co-expression genes were screened using the LinkedOmics database, and the GO enrichment analysis and KEGG pathway analysis were carried out. Results: The results showed that GABRE was highly expressed in colon cancer and indicated a poor prognosis (all P<0.05). The Venn diagram indicated that hsa-miR-370-3p targeted GABRE, and its expression was markedly increased in normal tissues (P<0.01). The expression of GABRE was positively correlated with the expressions of OGT and FAM156A genes, whereas negatively correlated with the expressions of ATP5A1 and MPDU1 genes (all P<0.05). GO biological process function and KEGG pathway enrichment analysis suggested that the GABRE gene may be involved in biological processes including protein dealkylation and regulation of cyclin-dependent protein kinase activity and enriched in taurine metabolism and NF-κB signaling pathway. Conclusions: GABRE gene is highly expressed in patients with colon cancer and indicates a poor prognosis, suggesting that the gene may serve as a potential novel target for the diagnosis and treatment of colon cancer.

Association of circulating long non-coding RNA MALAT1 in diagnosis, disease surveillance, and prognosis of acute ischemic stroke

We aimed to investigate the association of long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (lnc-MALAT1) with acute ischemic stroke (AIS), and its association with disease severity, inflammation, and recurrence-free survival (RFS) in AIS patients. One hundred and twenty AIS patients and 120 controls were recruited. Venous blood samples from AIS patients (within 24 h after symptoms onset) and controls (at entry to study) were collected to detect plasma lnc-MALAT1 expression by real-time quantitative polymerase chain reaction. AIS severity was assessed by the National Institutes of Health Stroke Scale (NIHSS) score. Plasma concentrations of inflammation factors (including C-reactive protein (CRP), tumor necrosis factor α (TNF-α), interleukin (IL)-6, IL-8, IL-10, IL-17, and IL-22) were measured and RFS was calculated. lnc-MALAT1 expression was decreased in AIS patients compared to controls, and it had a close correlation with AIS (AUC=0.791, 95% CI: 0.735-0.846). For disease condition, lnc-MALAT1 expression negatively correlated with NIHSS score and pro-inflammatory factor expression (including CRP, TNF-α, IL-6, IL-8, and IL-22), while it positively correlated with anti-inflammatory factor IL-10 expression. Furthermore, lnc-MALAT1 expression was elevated in AIS patients with diabetes. For prognosis, no statistical correlation of lnc-MALAT1 expression with RFS was found, while a trend for longer RFS was observed in patients with lnc-MALAT1 high expression compared to those with lnc-MALAT1 low expression.

Review of Eerdun-Wurile clinical research / Монголын эм зүй, эм судлал

Objective@#To summarize the clinical application of Zhenbao pills (Eerdun-Wurile) in recent years, so as to clarify its clinical efficiency and provide an effective basis for future research.@*Methods@#The clinical application of Zhenbao pills (Eerdun-Wurile) was classified and summarized by consulting the relevant literature.@*Results@#Mongolian medicine Eerdun-Wurile consists of many active ingredients such as <i>Sandalwood Padauk</i>, pearl, <i>Myristica fragrans, Calculus bovis</i>, Bufallo horn concentrated powder, <i>Gardenia jasminoides Ellis, Glycyrrhiza uralensis Fisch, Euphorbia humifusa, Catsia tora Linn, Chingma Abutilon Seed</i>, Resina Liquidambaris, <i>Carthamus tinctorius L, Eugenia caryophyllata Thunb, Cardamoj amomum, Semen Nigellae, Cuminum cyminum L, Piper longum Linn, Lygodium japonicum</i>, crab, medicine terminalia fruit, <i>MeLia toosendan Sieb.etZucc</i>, musk, white sandalwood, <i>Cinnamomum cassia Presl, Aucklandia lappa Decne, Inula helenium L, Amomum tsao-ko Crevostet Lemaire etc</i>. Mongolian medicine Eerdun-Wurile used for treat cardiovascular and cerebrovascular diseases, cerebral infarction, cerebral infarction and ischemic stroke, rheumatic heart disease, coronary heart disease, angina pectoris, rheumatism, kind rheumatism, cervical spondylosis, arthritis, insomnia, high blood pressure, epilepsy, skin diseases, diabetes, eye diseases.@*Conclusion@#Mongolian Medicine Eerdun-Wurile has remarkable curative effect on cardiovascu- lar and cerebrovascular diseases, brain infarction, cerebral infarction, ischemic stroke, diabetic diseases, rheumatism, bone diseases and so on. In this paper, the curative effect of this drug was summarized to provide reference for its clinical application.

Clinical analysis of Salvia ligustrazine injection on non-arteriti anterior ischemic optic neuropathy / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To study the effect and mechanism of salvia ligustrazine on non-arteriti anterior ischemic optic neuropathy.<p>METHODS: A total of 60 cases of non-arteriti anterior ischemic optic neuropathy were randomly divided into two groups. The control group(<i>n</i>=30 cases)was treated with oral prednisone acetate tablets and injection of compound anisodine. The experimental group(<i>n</i>=30 cases)was the same to the control group and added intravenous salvia ligustrazine, the period of treatment was 14d. The levels of IL-1β and TNF-α were detected by ELISA, and the protein expression levels of Bcl-2 and Caspase-3 were detected by Western blot. <p>RESULTS: The best corrected visual acuity was improved in both groups. The plasma levels of IL-1β and TNF-α were significantly lowed in the experimental group than the control group, and the difference between the two groups was statistically significant(<i>P</i><0.05). The protein expression of Bcl-2 and Caspase-3 in the two groups were different from those before treatment(<i>P</i><0.05), and the difference between the two groups was statistically significant(<i>P</i><0.05). <p>CONCLUSION: Salvia ligustrazine promotes the recovery of visual function by reducing the level of inflammation and inhibiting cell apoptosis in body.

Evolution and transition of source of Patrinia Herba and Patrinia Radix / 中国中药杂志

Both Patrinia Herba and Patrinia Radix are traditional Chinese herbal medicines. The herbal source and medicinal part of them are confusing in the herbal medicine market of China. To explore the evolution and transition of the herbal source and medicinal part of Patrinia Herba and Patrinia Radix, this paper systematically summarizes the record of the herbal source and medicinal part of them in ancient classics of herbal medicine in China. According to the findings, before Ming Dynasty, Patrinia Herba originated from the radix of the plants with yellow flowers of Patrinia. In Ming and Qing Dynasty, Patrinia Herba originates from the whole plant (including the radix)of the plant with white flowers of Patrinia. In Ming Dynasty, Patrinia Radix, stemming from the radix of the plants with yellow flowers of Patrinia, started to be used as a traditional Chinese herbal medicine, which had the same herbal source with that of Patrinia Herba before Ming Dynasty. Therefore, Patrinia Herba and Patrinia Radix can be seen as the same traditional Chinese herbal medicine, and the genuine of Patrinia Herba should be the radix and the whole herba of P. scabiosaefolia and P. heterophylla.

Clinical application of new mandibular molar intruding appliance / 口腔疾病防治

Objective@# To evaluate the effects and clinical application of molar intrusion with a new mandibular molar intrusion appliance.@*Methods @#7 patients who needed mandibular molars intrusion to correct open-bite were selected. To evaluate effects of molar intrusion, clinical examination, dental model analysis and aphalometric analysis by Winceph 7.0 software were used before and after intrusion treatment, SPSS 17.0 software was used for data analysis. @*Results @#All the mandibular molars were intruded successfully in an average time of 4.0 months. Cephalometric analysis before and after treatment showed that the average amount of mandibular first molars intrusion were (1.92 ± 0.44) mm．The vertical distance to the reference plane (MP plane) per and post treatment was statistically significant (P < 0.05). The average amount of maxillary first molar intrusion was (0.36 ± 0.25) mm, with no significant difference in vertical distances to reference plane (PP plane) (P > 0.05). No signs of root resorption was obsersed in radiograph of the intruded mandibular molars.@*Conclusion @#The new mandibular molar intrusion appliance has a remarkable clinical effect in correcting anterior open bite without obvious periodontal tissue damage and root resorption. teeth intrusion with the new appliance has a significant effect without apparent destruction of the periodontal tissue and root resorption.

The effect of unilateral Forsus appliance in the treatment of ClassⅡmalocclusion subdivision / 口腔疾病防治

Objective @# To assess the result of treatment in ClassⅡmalocclusion subdivision with unilateral Forsus appliance. @*Methods @#23 patients with ClassⅡmalocclusion subdivision were selected, who were treated with Straight wire fixed appliance in combination with unilateral Forsus appliances while another 27 patients were untreated as control group. Lateral cephalographs were taken before and after the comprehensive treatment, and the indicators of dental, skeletal, and soft tissue profile were measured.@*Results @#The Forsus appliance can correct ClassⅡmalocclusion subdivision through distalizing the upper teeth and moving the lower teeth mesially. Midline can be corrected at the same time. There was a statistically significant difference in the amount of tooth movement (P < 0.05). @*Conclusion @#Forsus appliance is an effective device for treating ClassⅡmalocclusion subdivision, which can induce significant dental and soft tissue changes.

Early diagnosis of sub-clinical stage of diabetic retinopathy / 国际眼科杂志(Guoji Yanke Zazhi)

AlM:To evaluate the early diagnosis of sub-clinic stage of diabetic retinopathy. METHODS: This was cross sectional study, multifocal retina electroretinogram ( mf-ERG ) , contrast sensitivity ( CS) and central retinal artery color Doppler examination were recorded from 30 cases ( 30 eyes ) matched control subjects, 35 cases (35 eyes) with type 2 diabetes mellitus (DM) without diabetic retinopathy ( NDR) and 38 cases ( 38 eyes ) with non-prolifera tive diabetic retinopathy ( NPDR) . One-way ANOVA and SNK-q test were used for data analysis. RESULTS: P1 response density of NDR patients were found decrease, N1 implicit time were delayed. Which were related with the degree of retinopathy (P0. 05), The differences between normal group, NDR group and NPDR group were found statistically significant (P CONCLUSlON: mf-ERG and CS are sensitive indexes for early evaluation of visual function in patients with diabetes mellitus, with development of the disease, CRA blood flow also appears to decline.

Regular intermittent bolus provides similar incidence of maternal fever compared with continuous infusion during epidural labor analgesia

To compare the effects of regular intermittent bolus versus continuous infusion for epidural labor analgesia on maternal temperature and serum interleukin-6 [IL-6] level. This randomized trial was performed in Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu Province, China between October 2012 and February 2014. Either regular intermittent bolus [RIB, n=66] or continuous infusion [CI, n=66] was used for epidural labor analgesia. A bolus dose [10 ml of 0.08% ropivacaine + 0.4 microg·ml[-1] sufentanil] was manually administrated once an hour in the RIB group, whereas the same solution was continuously infused at a constant rate of 10 ml·h[-1] in the CI group. Maternal tympanic temperature and serum IL-6 level were measured hourly from baseline to one hour post partum. The incidences of fever [>/= 38°C] were calculated. The incidence of maternal fever was similar between the 2 groups. There was a rising trend in mean temperature over time in both groups, but no statistical difference was detected between the groups at respective time points; maternal serum IL-6 showed similar changes. Compared with continuous infusion, regular intermittent bolus presents with the same incidence of maternal fever for epidural labor analgesia. Interleukin-6 elevation could be involved in mean maternal temperature increase

Influence of the peritoneal lavage with bupivacaine on the survival and resistance of colonic anastomoses performed under fecal peritonitis in rats

PURPOSE: To evaluate the effects of peritoneal lavage with bupivacaine on survival and initial resistance of anastomosis on distal colon, performed under peritonitis or not. METHODS: Forty rats, weighing from 300 to 350g (321.29±11.31g), were randomly divided in four groups and underwent laparotomy and anastomosis on the distal colon six hours after induction of peritonitis by intraperitoneal injection of autologous fecal material or not. Group 1: No peritonitis and lavage with 3ml NS; Group 2: No peritonitis and lavage with 8 mg.kg-1 (± 0.5 mL) of 0.5% bupivacaine added to 2.5 mL of NS; Group 3: Peritonitis and lavage with 3ml NS; Group 4: Peritonitis and lavage with 8 mg.kg-1 (± 0.5 mL) of 0.5% bupivacaine added to 2.5 mL of NS. Necropsies were performed on the animals that died and the time of death was recorded. Surviving animals were submitted to euthanasia on the fifth post-operative day and Total Energy of Rupture biomechanical test (TER) was applied. RESULTS: Group 4 showed survival increase compared to Group 3, without statistical significance. Group 3 presented the smallest average TER, with statistical significance. CONCLUSION: Peritonitis increased mortality and influenced negatively the resistance of colic anastomosis in rats. Peritoneal lavage with bupivacaine increased anastomotic resistance.

Analysis of biomechanical parameters extracted from anorectal manometry of fecally-continent and incontinent patients / Análise de parâmetros biomecânicos extraídos da manometria anorretal de pacientes continentes e com incontinência fecal / Análisis de parámetros biomecánicos extraídos de la manometría recto-anal de pacientes con continencia e incontinencia fecal

OBJECTIVE: to evaluate the average resulting area from the Pressure x Time curves resulting from the manometric anorectal exam and compare it with the support capacity of voluntary squeeze. Materials and METHODS: the data set was represented by data from 11 exams from continent patients and eight exams from patients with grade III fecal incontinence. The manometric curves were delineated, and the areas and support capacity of voluntary squeeze calculated, by means of the R computer language and the algorithm developed. RESULTS: the resulting averages for support capacity of voluntary squeeze in continent patients and patients with grade III fecal incontinence were 33.07 seconds and 30.76 seconds (p>0.05) and the averages for area were 2362.04 mmHg x second and 947.92 mmHg x second (p<0.05), respectively. CONCLUSION: the average resulting area is able to differentiate continent patients from incontinent and is shown to be a possible parameter in the analysis of biomechanical behavior related to the mechanisms of anorectal continence.

OBJETIVO: avaliar a área média resultante, proveniente das curvas pressão versus tempo, pertencentes ao exame manometria anorretal e confrontá-la com a capacidade de sustentação. MATERIAIS E MÉTODOS: a casuística foi representada por dados de 11 exames de pacientes continentes e oito exames de pacientes com incontinência fecal grau III. Por meio da linguagem computacional R e do algoritmo desenvolvido foram delineadas as curvas manométricas e calculadas as áreas e capacidades de sustentação. RESULTADOS: as médias resultantes da capacidade de sustentação de pacientes continentes e com incontinência fecal grau III foram 33,07 segundos e 30,76 segundos (p>0,05) e as da área, 2362,04 mmHg x segundo e 947,92 mmHg x segundo (p<0,05), respectivamente. CONCLUSÃO: a área média resultante foi capaz de diferenciar os pacientes continentes dos incontinentes e demonstra ser um possível parâmetro na análise do comportamento biomecânico, relacionado aos mecanismos de continência anorretal.

OBJETIVO: evaluar el área media resultante proveniente de las curvas Presión versus Tiempo pertenecientes al examen manometría rectal y afrontarla con la capacidad de sustentación. Materiales y MÉTODOS: la casuística fue representada por datos de 11 exámenes de pacientes moderados y ocho exámenes de pacientes con incontinencia fecal grado III. Por medio del lenguaje computacional R y del algoritmo desarrollado fueron delineadas las curvas manométricas y calculadas las áreas y capacidades de sustentación. RESULTADOS: los medios resultantes de la capacidad de sustentación de pacientes moderados y con incontinencia fecal grado III fueron 33,07 segundos y 30,76 segundos (p>0,05) y las de la área, 2362,04 mmHg x según y 947,92 mmHg x según (p<0,05), respectivamente. CONCLUSIÓN: el área media resultante fue capaz de diferenciar los pacientes continentes de los incontinentes y demuestra ser un posible parámetro en el análisis del comportamiento biomecánico relacionado a los mecanismos de continencia rectal.

Prototype of a computer system for managing data and video colonoscopy exams

OBJECTIVE: Develop a prototype using computer resources to optimize the management process of clinical information and video colonoscopy exams. MATERIALS AND METHODS: Through meetings with medical and computer experts, the following requirements were defined: management of information about medical professionals, patients and exams; video and image captured by video colonoscopes during the exam, and the availability of these videos and images on the Web for further analysis. The technologies used were Java, Flex, JBoss, Red5, JBoss SEAM, MySQL and Flamingo. RESULTS AND DISCUSSION: The prototype contributed to the area of colonocospy by providing resources to maintain the patients' history, tests and images from video colonoscopies. The web-based application allows greater flexibility to physicians and specialists. The resources for remote analysis of data and tests can help doctors and patients in the examination and diagnosis. CONCLUSION: The implemented prototype has contributed to improve colonoscopy-related processes. Future activities include the prototype deployment in the Service of Coloproctology and the utilization of this model to allow real-time monitoring of these exams and knowledge extraction from such structured database using artificial intelligence. (AU)

OBJETIVO: Desenvolver um protótipo por meio de recursos computacionais para a otimização de processos de gerenciamento de informações clínicas e de exames de videocolonoscopia. MATERIAIS E MÉTODOS: Por meio de reuniões com especialistas médicos e computacionais, definiram-se os seguintes requisitos: gestão de informações sobre profissionais médicos, pacientes e exames complementares; aquisição dos vídeos e captura de imagens a partir do videocolonoscópio durante a realização desse exame, e a disponibilidade por meio da Web para análise posterior dessas imagens. As tecnologias aplicadas foram: Java, Flex, JBOSS, Red5, JBOSS SEAM, MySQL e Flamingo. RESULTADOS E DISCUSSÃO: O protótipo contribuiu para a área de colonocospia disponibilizando recursos para manutenção de histórico de pacientes, exames e imagens. O acesso à aplicação, por meio de browser, permite maior flexibilidade aos médicos e especialistas. Os recursos para análise remota de dados e exames podem auxiliar médicos e pacientes na realização de exames e diagnósticos. CONCLUSÃO: O protótipo implementado contribuiu para melhoria de processos relacionados a exames de videocolonoscopia. Trabalhos futuros incluem implantação do protótipo no serviço de coloproctologia, bem como a extensão do modelo para o acompanhamento dos exames em tempo real e extração de conhecimento dessa base de dados estruturada por meio de inteligência artificial. (AU)

Effect of down-regulation of histone deacetylase 2 protein expression on cell proliferation and cell cycle in cervical carcinoma / 中华病理学杂志

<p><b>OBJECTIVE</b>To study the effect of down-regulation of histone deacetylase 2 (HDAC2) expression on cell proliferation and cell cycle in cervical carcinoma cell lines HeLa.</p><p><b>METHODS</b>HDAC2 siRNA and control siRNA were transfected to HeLa cells. CCK-8 and flow cytometry were used to analyze the changes of cell proliferation and cell cycle, respectively. Western blot was employed to detect the changes of cell proliferation and cell cycle-related proteins.</p><p><b>RESULTS</b>HDAC2 siRNA significantly down-regulated the expression of HDAC2 protein in HeLa cells, resulting in marked inhibition of cell proliferation. In addition, the percentage of cells in G(0)/G(1) phase in HDAC2 siRNA group (63.3% ± 2.0%) was significantly higher than that in untreated group (29.3% ± 1.7%) or control siRNA group (29.4% ± 1.7%), F = 354.181, P = 0.000. Furthermore, Western blot demonstrated that down-regulation of HDAC2 expression decreased the expression of cyclin D1, cyclin E and CDK2 proteins but increased the expression of p21 protein.</p><p><b>CONCLUSIONS</b>Down-regulation of HDAC2 expression mediates proliferation inhibition and cell cycle arrest. It is associated with decrease in cyclin D1, cyclin E and CDK2 protein expression and increase in p21 protein expression.</p>

A cross-sectional study about the effects of residential exposure to power transmission line on human nervous system symptoms and hematology / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To investigate the possible effects on nervous system and health condition under the exposure to electromagnetic field.</p><p><b>METHODS</b>Take the resident around the power transmission line as the objects and were divided into 3 groups by the distance from the power transmission line 20 m, 100 m and 500 m, respectively. Some living conditions and health conditions were recorded by face-to-face the questionnaire survey, and Hematological indices of each groups were examined including IgG, IgM, leukocyte formulae, erythrocyte, hemoglobin and platelet.</p><p><b>RESULTS</b>There was no significant difference in each group, according exposure of daily life, such as drinking and smoking (P > 0.05). Compared with the each distance groups, it was presented significant difference between the distance from the power transmission line and the incidence of headache or dizziness, insomnia and easy weary and so on (P < 0.05). In hematology aspect, with the horizontal distance from the power transmission line decreasing, PLT level of residents was reductive and the difference was statistically significant (P < 0.001), whereas leukocyte formulae, erythrocyte, hemoglobin, IgG and IgM had no significant difference among each group (P < 0.05).</p><p><b>CONCLUSION</b>Closely exposure to electromagnetic field may induce headache and so on and decrease the level of PLT.</p>

Prototype system to manage data on coloproctology surgery

OBJECTIVE: To develop a prototype system to manage data on coloproctology surgery, aiming at Data Quality (DQ) and the adoption of a DQ monitoring process, which is nonexistent in most biomedical systems. METHODS: The construction of the prototype was separated into five steps: analysis of an existing system (legacy), the analysis of requirements and specifications for the new prototype, the development of the model, definition of technologies and the development of a prototype. RESULTS: The analysis of the legacy system revealed several limitations and inconsistencies, which can result in problems concerning the DQ. Therefore, actions to prevent these problems are already being executed at the step of developing the prototype, such as the creation of interactive and more elaborate interfaces, the use of validation mechanisms on data fields and the proposal of a process to monitor inconsistencies and incompleteness in patients' data. CONCLUSION: The adoption of DQ mechanisms on system development results in building a reliable and consistent database, to assist tasks such as management, scientific research and future intelligent data analysis methods. Future work includes subjective evaluations of DQ indicating the adequacy of the prototype for the users' needs. (AU)

OBJETIVO: Desenvolvimento de um protótipo para gerenciamento de dados de cirurgia coloproctológica, visando à Qualidade de Dados (QD), e a adoção de um processo de monitoramento da QD, inexistente na maioria dos sistemas biomédicos. MÉTODOS: A construção do protótipo foi dividida em cinco etapas: análise de um sistema existente (legado), levantamento dos requisitos para o novo protótipo, elaboração de modelos, definição das tecnologias e desenvolvimento do protótipo. RESULTADOS: A análise do sistema legado revelou diversas limitações e inconsistências que podem resultar em problemas de QD. Sendo assim, medidas para prevenir esses problemas estão sendo adotadas, já na etapa do desenvolvimento do protótipo, como a criação de interfaces mais elaboradas e interativas, a utilização de mecanismos de validação dos campos de dados e a proposta de um processo para monitoramento das inconsistências e incompletudes dos dados dos pacientes. CONCLUSÃO: A adoção de medidas de QD no desenvolvimento de sistemas resulta na construção de uma base de dados confiável e consistente, contribuindo com as tarefas de gerenciamento, pesquisas científicas e futuras aplicações de métodos de análise inteligente de dados. Trabalhos futuros incluem avaliações subjetivas de QD que indiquem o nível de adequação do protótipo às necessidades dos usuários. (AU)

Protótipo de um sistema de gerenciamento de protocolos de câncer colorretal / Management system prototype of colorectal cancer protocols

OBJETIVO: desenvolvimento de um protótipo de sistema de cadastro e controle de protocolos de Câncer Colorretal, com a finalidade de armazenar um conjunto abrangente de dados de forma estruturada para posterior aplicação de métodos de análise inteligente de dados. MATERIAL E MÉTODO: o protótipo foi construído em cinco etapas: análise do domínio de Câncer Colorretal, definição de requisitos básicos (operacionais e informacionais), projeto do sistema, construção do sistema usando tecnologias open source e avaliação do sistema com o apoio de especialistas. RESULTADOS E DISCUSSÃO: durante o desenvolvimento, o protótipo foi avaliado por especialistas quanto à estrutura do conjunto de dados, à coerência de organização das informações, à validade das funcionalidades implementadas e ao atendimento aos requisitos básicos definidos. CONCLUSÃO: de acordo com os especialistas usuários, o desenvolvimento do protótipo completou a primeira etapa do projeto de desenvolvimento de um sistema em nível de 100 por cento de satisfação e, atualmente, está sendo aplicado em cadastros de dados reais. Na próxima etapa será consolidado e implantado o sistema completo, atendendo a requisitos de segurança com suporte a multiusuários.

OBJECTIVE: to develop a prototype of a Colorectal Cancer records management system, aiming to store an embracing structured dataset for later application of intelligent data analysis methods. MATERIAL AND METHOD: the prototype was developed in five stages: Colorectal Cancer domain analysis, definition of basic requirements (operational and informational), system design, system construction using open source technologies and system evaluation supported by domain experts. RESULTS AND DISCUSSION: during development, the prototype dataset structure, coherence of information structure, validity of functions implemented and the attendance to the basic requirements define, were evaluated by domain experts. CONCLUSION: according to the experts evaluation, the prototype development completed the first stage of the project of developing the system with a 100 percent satisfaction rate and is now being used into recording real data. In the next stage it will be finished and deployed the complete system, attending to security requirements and multiuser support.