ABSTRACT
OBJECTIVE:To build up a method for determining the concentration of zaleplon in human plasma by HPLC. METHODS:The Kromasil-C 8 column was used and mobile phase consisted of methanol-water(65∶35);the flow rate was1.0ml/min,fluorescence excitation wave-length was233nm;fluorescence emission wave-length was460nm.RESULTS:The calibration curve revealed linearity in the concentration range of0.42～73.92ng/ml(r=0.9999)with a regressive equation of Y=1.870?147.The average recovery was99.47%.The with-day and between-day RSDs were4.9%and4.73%respec?tively.CONCLUSION:The method is accurate,reliable and highly sensitive so it can be used in study of pharmacokinetics of zaleplon.
ABSTRACT
OBJECTIVE:Dual-wavelength spectrophotometry was developed for the determination of ciprofloxacin lactate in its eye drops.METHODS:Detection was performed at wavelength 237nm and 279nm with 0.1mol/L HCl as diluent.RESU_LTS:The assay was linear for ciprofloxacin lactate in the concentration range of 3～9?g/ml,r=0.9 998;The mean recovery was 101.6%,RSD was 2.16%.CONCLUSION:This method is simple,quick,accurate and suitable for the determination of ciprofloxacin lactate in its eye drops.