ABSTRACT
OBJECTIVE:To establish rapid method for content determination of cryptotanshinone in Salvia miltiorrhiza. METHODS:The content of cryptotanshinone in sample was determined by HPLC(as reference value). AOTF-NIDRS combined with PLS was used to establish quantitative correction model for the content of cryptotanshinone in S. miltiorrhiza. According to the results of content determination of cryptotanshinone in samples,35 samples of medicinal material were collected. First-order derivative combined with smoothing filter coefficient method was used to pretreat spectrum,and optimal band range for content determination of cryptotanshinone in sample ranged 1 250-2 150 nm. RESULTS:Methodology validation of content determination of cryptotanshinone in sample was in line with the requirements. Correction mean square deviation of quantitative correction model of cryptotanshinone was 0.014 6,and predicted mean square deviation was 0.022 3,coefficient of association was 0.976 6. The internal verification deviation was 2.41% and the external verification deviation was 4.06%. CONCLUSIONS:This method is rapid,accurate,simple and pollution-free.It can be used for rapid content determination of cryptotanshinone in S.miltiorrhiza.
ABSTRACT
Objective To evaluate the clinical value of detection of urinary interleukin-8（IL-8） in patients with lupus nephritis （LN）.Methods Urinary levels of IL-8 were determined by using sandwich ELISA in 38 LN patients.Results In the LN patients during the stage of normal renal function and diminished renal reserve stage， urinary levels of IL-8 were significantly higher than those in healthy controls（P＜0.001），and urinary levels of IL-8 in the patients during diminished renal reserve stage were significantly higher than those in the patients during normal renal function stage（P＜0.01）.High IL-8 levels in LN patients were associated with high levels of erythrocyte sedimentation rate and lower levels of C3 complement components. Urinary levels of IL-8 in LN patients with microscopic leukocyturia were significantly higher than those without microscopic leukocyturia. In LN patients treated with cyclophosphamide （CTX） and prednisone， urinary IL-8 levels of post-treatment were significantly lower than those of pre-treatment（P＜0.01）. Conclusions Urinary levels of IL-8 may be a useful indicator for monitoring disease activity and degree of renal damage in LN. The mechanism of noninfective leukocyturia may be associated with the elevation of urinary IL-8 levels in LN. CTX and prednisone may have a significant effect on inhibiting the production of IL-8 in the immunocytes and renal intrinsic cells.
ABSTRACT
Objective:To investigate role of interleukin 6(IL 6) in the pathogenesis of lupus nephritis(LN) and effect of cyclophosphamide pulse therapy on production of IL 6.Methods:Serum IL 6 levels of pre and post therapy were determined by using sandwich ELISA in LN patients.Results:In the active LN patients,serum levels of IL 6 were significantly higher than those in inactive LN patients and healthy controls(268.26?79 64 ng/L vs 62.40?45 23 ng/L,268 26?79 64 ng/L vs 50.36?42 75 ng/L,P16 g/L were significantly higher than those in other active LN patients (329.58?94.21 ng/L vs 233.22?43.56 ng/L,P