ABSTRACT
<p><b>OBJEVTIVE</b>To investigate the expression of transient receptor potential lvanilloidreceptor 4 (TRPV4) protein in pemphigus vulgaris (PV), bullous pemphigoid (BP), dermatitis herpetiformis (DH), and epidermolysis bullosa acquisita (EBA), and explore the role of TRPV4 in the pathogenesis of these diseases.</p><p><b>METHODS</b>TRPV4 protein in normal skin tissues and lesions of PV, BP, DH, and EBA were detected with immunohistochemistry.</p><p><b>RESULTS</b>The positivity rate of TRPV4 protein expression was 61.90% in PV, 81.81% in BP, 72.22% in DH, and 68.42% in EBA. TRPV4-positive rates in these lesions were significantly lower than the rate in normal skin tissues (93.33%) and also differed significantly among these lesions (PV<EBA<DH<BP).</p><p><b>CONCLUSIN</b>Low TRPV4 expressions may affect the formation and reconstitution of skin connection. TRPV4 may play an role in the occurrence and development of autoimmune bullous skin disorders.</p>
Subject(s)
Humans , Dermatitis Herpetiformis , Metabolism , Diagnosis, Differential , Epidermolysis Bullosa Acquisita , Metabolism , Pemphigoid, Bullous , Metabolism , Pemphigus , Metabolism , Skin , Pathology , TRPV Cation Channels , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of topical treatment with adenovirus-mediated promyelocytic leukemia gene (PML) gene in a psoriasis-like mouse model.</p><p><b>METHODS</b>The effect of adenovirus-mediated PML gene on the granular layer of mouse tail scale epidermis and epithelial mitosis were observed on longitudinal histological sections prepared from the tail skin and vaginal epithelium of the mice.</p><p><b>RESULTS</b>Adenovirus-mediated PML gene significantly inhibited mitosis of mouse vaginal epithelial cells and promoted the formation of granular layer in mouse tail scale epidermis.</p><p><b>CONCLUSION</b>The therapeutic effect of PML gene in the psoriasis-like mouse model may be associated with increased granular cells and suppressed epidemic cell proliferation.</p>
Subject(s)
Animals , Female , Male , Mice , Adenoviridae , Genetics , Administration, Topical , Cell Proliferation , Disease Models, Animal , Epithelial Cells , Cell Biology , Genetic Vectors , Mice, Inbred Strains , Mitosis , Nuclear Proteins , Genetics , Promyelocytic Leukemia Protein , Psoriasis , Therapeutics , Skin , Cell Biology , Transcription Factors , Genetics , Tumor Suppressor Proteins , Genetics , Vagina , Cell BiologyABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of promyelocytic leukaemia (PML) protein of PML protein in Bowen's disease (BD), skin squamous cell carcinoma (SCC) and basal cell carcinoma (BCC) and explore the role of PML in the pathogenesis of these diseases.</p><p><b>METHODS</b>PML protein in normal skin tissues and lesions of Bowen's disease, SCC and BCC were detected with immunohistochemistry.</p><p><b>RESULTS</b>Normal skin tissues did not express PML protein. In BCC, PML showed rather low expressions in the skin lesions (8.69% in cell nuclei and 4.35% in cytoplasm). The lesions in BD and SCC (grade I and II) showed obvious overexpression of PML protein in the cell nuclei and cytoplasm, and its expression in the cell nuclei of these lesions was significantly higher than that in grade III-IV SCC.</p><p><b>CONCLUSION</b>PML protein may play an important role in the early stage of SCC, and its overexpression may contribute to the carcinogenesis and metastasis of SCC.</p>