ABSTRACT
Objective To evaluate the clinical value of detection of urinary interleukin-8（IL-8） in patients with lupus nephritis （LN）.Methods Urinary levels of IL-8 were determined by using sandwich ELISA in 38 LN patients.Results In the LN patients during the stage of normal renal function and diminished renal reserve stage， urinary levels of IL-8 were significantly higher than those in healthy controls（P＜0.001），and urinary levels of IL-8 in the patients during diminished renal reserve stage were significantly higher than those in the patients during normal renal function stage（P＜0.01）.High IL-8 levels in LN patients were associated with high levels of erythrocyte sedimentation rate and lower levels of C3 complement components. Urinary levels of IL-8 in LN patients with microscopic leukocyturia were significantly higher than those without microscopic leukocyturia. In LN patients treated with cyclophosphamide （CTX） and prednisone， urinary IL-8 levels of post-treatment were significantly lower than those of pre-treatment（P＜0.01）. Conclusions Urinary levels of IL-8 may be a useful indicator for monitoring disease activity and degree of renal damage in LN. The mechanism of noninfective leukocyturia may be associated with the elevation of urinary IL-8 levels in LN. CTX and prednisone may have a significant effect on inhibiting the production of IL-8 in the immunocytes and renal intrinsic cells.
ABSTRACT
Objective:To investigate role of interleukin 6(IL 6) in the pathogenesis of lupus nephritis(LN) and effect of cyclophosphamide pulse therapy on production of IL 6.Methods:Serum IL 6 levels of pre and post therapy were determined by using sandwich ELISA in LN patients.Results:In the active LN patients,serum levels of IL 6 were significantly higher than those in inactive LN patients and healthy controls(268.26?79 64 ng/L vs 62.40?45 23 ng/L,268 26?79 64 ng/L vs 50.36?42 75 ng/L,P16 g/L were significantly higher than those in other active LN patients (329.58?94.21 ng/L vs 233.22?43.56 ng/L,P