ABSTRACT
Objective To investigate the effects of β1-adrenergic receptor autoantibodies (β1-AA) on the proliferation of different subtypes of T lymphocytes in patients with heart failure .Methods β1-AA-positive IgG antibodies isolated from patients with heart failure were purified by using affinity chromatog -raphy.CD3 +CD4 +T and CD3 +CD8 +T lymphocytes were sorted by flow cytometry analysis .The prolifera-tion of different subtypes of T lymphocytes was tested by using CCK-8 kit.Tests for lactate dehydrogenase ( LDH) level and cell apoptosis were performed to evaluate T lymphocytes damage .Results The prolifera-tion of activated lymphocytes was inhibited by β1-AA isolated from the serum of patients with heart failure in a concentration dependent manner , but it could be blocked by β1 receptor blocker .The damage of lympho-cytes induced by β1-AA was increased.Moreover, β1-AA promoted the necrosis and apoptosis of CD 3 +CD4 +T and CD3 +CD8 +T lymphocytes and thus inhibited the proliferation of them .Conclusion β1-AA isolated from the serum of patients with heart failure inhibited the proliferation of CD 3 +CD4 +T and CD3 +CD8 +T lymphocytes through increasing the necrosis and apoptosis of them .This study suggests that β1-AA might induce immune disorders in addition to causing pathological changes in heart tissues .
ABSTRACT
To study the effect of genistein on the proliferation of cardiac fibroblasts(CF), CFs were cultured from neonatal rat hearts, DNA synthesis of the cells was determined by incorporation of ［3H］TdR into DNA, the cell cycle was measured by flow cytometric analysis. Genistein(0.5－50 μmol*L-1) attenuated 2.5% fetal calf serum-induced proliferation of CF in concentration-dependent manner. Genistein(50 μmol*L-1) arrested CF cell progression at G2/M phase. The results suggest that genistein be a potential substance for treatment of cardiac fibrosis.
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OBJECTIVE:To establish a method for determination of the contents of chlorogenic acid and baicalin in Shiwuwei Xiaoyanzhike oral liquid .METHODS:HPLC was used,the mobile phase was acetonitrile - acetic acid,the flow rate was 0.8ml/ min and the detecting wavelengths were 326nm and 274nm.RESULTS: The recoveries of chlorogenic acid and baicalin were 96.8% - 104.5% , 94.9% -97.7% and RSD were 1.09% -3.12% , 1.19% -1.76% (n = 3), respectively .CONCLUSION: The method is simple and feasible with a good reproducibility, the RESULTS: can provide a basis for establishing the quality standard of this oral liquid.
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The changes in flow properties of blood draining-from ischemic area and from systemic circulation causad by acute coronary occlusion were studied in 21 anesthe- tized open-chest dogs and the role of cardiac-cardiac sympathetic reflex in these changes was analysed. The main results obtained were as in the following: 1. The obvious hemorhoblogic changes were detected at the early phase of myocardial ischemia and the more significant change in high shear viscosity of whole blood was observed in coronary venous blood draining from ischemic area; 2. Topical application of lidocaine to the ischemic area of the heart virtually abolished the hemorheologie changes in systemic venous blood caused by coronary occlnsion, but had no significant effect on the change in high shear viscosity of blood draining from the ischemic area; 3. Bilateral section of stellate ganglia had no effect on the changes in hemorheologe following coronary occlusion. The results mentioned above suggested that increased activity of cardiac sympathetic nerves which originated from ischemic area had an important role in causing hemorheologic changes following coronary occlusion.
ABSTRACT
Following acute occlusion of the left anterior desending coronary artery, the significant and constant hemorrheologic changes were observed in blood draining from ischemic area. At the 40-min of occlusion, the tow shear viscosity (r=1.15 s~(-1)) of whole blood increased from the preocclusion value of 39?7.9m Pa.s to 58?8.4 mPa.s (P
ABSTRACT
The effects of decreasing .blood viscosity through hemodilution on coronary collateral flow and myocardial injury in the.early phase of myocardial ischomia were studied in 21 open-chest anaesthetized dogs. It was shown that after 30 rain of coromary occlusion the low-shear viscosity of the whole blood (?bl) began to increase gradually, while the collateral flow (CF) decreased concomitantly. At the 75-min postocclusion, ?bl increased by 18.1?3.9%, CF decreased by 24.1 ?4.6%, ST-segment of epicardial electrogram elevated significantly and the ultrastructural changes were characterized by severe mitochondrial damage, autolysis of myofilaments and blood cell obstruction in the micro vessel etc. In the hemodilution group, the chamges i1~ ~qbl and CF of the first 30-min postocclusion were quite the same as that of the control group. Following mild isovolemic hemodilution performed at the 30-min postocclusion, all of the ischemic changes improved significantly. At the 75-min postocclusion, contrary to the control group, ?bl decreased by 25.5?3.9% and CF increased by 54,7?10.8% as compared to the control value, the previously elevated ST segment decreased markedly and the ultrastructural injury alleviated to a great degree as well. The experimental results suggest that the deleterious hemorheologic changes in the early phase of myocardial ischemia is a major factor of aggravating ischemic myocardial injury and the decrese in blood viscosity through hemodilution may have protective effect on ischemic myocardium by increasing the collateral flow.