ABSTRACT
<p><b>OBJECTIVE</b>To investigate the regulatory effect and significance of transcription factor E2F1 on X-ray repair cross2 complementing 1 (XRCC1).</p><p><b>METHODS</b>Saos2 cells were transfected with the E2F1 expression vectors (tet-E2F1) and mutated E2F1 expression vectors (tet-132E). XRCC1 promotor luciferase reporter vector was constructed and transfected into Saos2 cells together with E2F1, E2F2, E2F3 and E2F4 expression vectors at different amount. The cells were collected 36 hours post-transfection for luciferase assays and absorbance was read at 570 nm.</p><p><b>RESULTS</b>Cotransfection of increasing amounts of E2F1 expression vector with the XRCC1 promoter-luciferase reporter caused a dose-dependent increase in luciferase activation. In contrast, DNA binding incompetent E2F1 (132E) could not activate the XRCC1 promoter-luciferase reporter.</p><p><b>CONCLUSION</b>E2F1 could upregulate endogenous XRCC1 expression and stimulate the XRCC1 promoter.</p>
Subject(s)
Humans , Cell Line, Tumor , DNA-Binding Proteins , Genetics , Metabolism , E2F1 Transcription Factor , Genetics , Metabolism , Gene Expression , Genes, Reporter , Promoter Regions, Genetic , Protein Binding , Up-Regulation , X-ray Repair Cross Complementing Protein 1ABSTRACT
<p><b>OBJECTIVE</b>Analyze Naive and Mermory T cell subsets in HIV/AIDS patients and investigate their relationship with disease development.</p><p><b>METHODS</b>T cell subsets from 15 normal control subjects, 79 HIV/ AIDS patients were detected by FCM.</p><p><b>RESULTS</b>With diesase progression, CD4+ Naive cell counts and ratio was both decreased obviously (P < 0.001); CD4+ Tcm cell counts was significantly decreased (P < 0.001), CD4+ Tcm cell ratio was obviously higher (P = 0.002); CD4 TEM cell ratio was obviously increased( P < 0.001); CD8+ T Naive cell counts and ratio was also decreased obviously (P < 0.05); CD8+ T(CM), T(EM), T(EMRA) are not significantly different.</p><p><b>CONCLUSIONS</b>The peripheral lymphocyte subsets in HIV/AIDS patients changed obviously. The counts of naive T cell decreased, while the proportion of memory T cell increased significantly. It will help to understand pathogenesis of HIV.</p>
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Acquired Immunodeficiency Syndrome , Allergy and Immunology , Virology , CD4 Lymphocyte Count , Case-Control Studies , HIV , Allergy and Immunology , T-Lymphocyte Subsets , Allergy and ImmunologyABSTRACT
<p><b>OBJECTIVE</b>To highly express TAT-HBX-EGFP fusion protein and study its distribution in mouse liver.</p><p><b>METHODS</b>TAT-HBX-EGFP recombinant vector was constructed and fusion protein was induced by IPTG and expression in BL21; fusion protein was purified by Ni-NTA argarose, then injected into the peritoneal cavity of the mice. Distribution of fusion protein was observed by immunofluorescence.</p><p><b>RESULTS</b>TAT-HBX-EGFP was highly expression in E. coli; HBX could be induced into mouse liver by TAT.</p><p><b>CONCLUSION</b>HBX protein could be induced into mouse liver by TAT induced peptide.</p>