GC-MS analyses of the volatiles of Houttuynia cordata Thunb.

GC-MS is the basis of analysis of plant volatiles. Several protocols employed for the assay have resulted in inconsistent results in the literature. We developed a GC-MS method, which were applied to analyze 25 volatiles [alpha- pinene, camphene, beta-pinene, 2-methyl-2-pentenal, myrcene, [+]-limonene, eucalyptol, trans-2-hexenal, gamma-terpinene, cis- 3-hexeneyl-acetate, 1-hexanol, alpha-pinene oxide, cis-3-hexen-1-ol, trans-2-hexen-1-ol, decanal, linalool, acetyl-borneol, beta- caryophyllene, 2-undecanone, 4-terpineol, borneol, decanol, eugenol, isophytol and phytol] of Houttuynia cordata Thunb. Linear behaviors for all analytes were observed with a linear regression relationship [r[2]>0.9991] at the concentrations tested. Recoveries of the 25 analytes were 98.56-103.77% with RSDs <3.0%. Solution extraction [SE], which involved addition of an internal standard, could avoid errors for factors in sample preparation by steam distillation [SD] and solidphase micro extraction [SPME]. Less sample material [[almost equal to] 0.05g fresh leaves of H. cordata] could be used to determine the contents of 25 analytes by our proposed method and, after collection, did not affect the normal physiological activity or growth of H. cordata. This method can be used to monitor the metabolic accumulation of H. cordata volatiles

Quality evaluation of Houttuynia cordata Thunb. by high performance liquid chromatography with photodiode-array detection [HPLC-DAD]

A new, validated method, developed for the simultaneous determination of 16 phenolics [chlorogenic acid, scopoletin, vitexin, rutin, afzelin, isoquercitrin, narirutin, kaempferitrin, quercitrin, quercetin, kaempferol, chrysosplenol D, vitexicarpin, 5-hydroxy-3,3',4',7-tetramethoxy flavonoids, 5-hydroxy-3,4',6,7-tetramethoxy flavonoids and kaempferol-3,7,4'-trimethyl ether] in Houttuynia cordata Thunb. was successfully applied to 35 batches of samples collected from different regions or at different times and their total antioxidant activities [TAAs] were investigated. The aim was to develop a quality control method to simultaneously determine the major active components in H. cordata. The HPLC-DAD method was performed using a reverse-phase C[18] column with a gradient elution system [acetonitrilemethanol-water] and simultaneous detection at 345 nm. Linear behaviors of method for all the analytes were observed with linear regression relationship [r[2]>0.999] at the concentration ranges investigated. The recoveries of the 16 phenolics ranged from 98.93% to 101.26%. The samples analyzed were differentiated and classified based on the contents of the 16 characteristic compounds and the TAA using hierarchical clustering analysis [HCA] and principal component analysis [PCA]. The results analyzed showed that similar chemical profiles and TAAs were divided into the same group. There was some evidence that active compounds, although they varied significantly, may possess uniform anti-oxidant activities and have potentially synergistic effects

Chemical constituents from Viscum nudum and their accelerating PC12 cell differentiation / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate chemical constituents of the stem of Viscum nudum and their bioacyivity.</p><p><b>METHOD</b>The major chemical constituents were isolated from the AcOEt-solved part of ethanol-extract of the plant by column chromatography and the active screening test in vitro were taken out for looking for compounds to acccelerate PC12 cell differentiation.</p><p><b>RESULT</b>5 compounds were identified as eriodictyol (1), 5, 7-dihydroxy-3', 4'-dimethoxy flavanone (2), oleanolic (3), 5, 7-dihydroxychromone (4) and homeriodictyol (5) by spectral evidences, in which homeriodictyol (5) had acceleration differentiation to PC12 cell.</p><p><b>CONCLUSION</b>All compounds were obtained from this plant for the first time, and bioactive constituent was observed in the AcOEt-solved part.</p>

Study on chemical constituents from Incarvillea arguta and their accelerating PC-12 cell differentiation / 中国中药杂志

<p><b>OBJECTIVE</b>To study chemical constituents of Incarvillea arguta and their accelerating PC-12 cell differentiation.</p><p><b>METHOD</b>The constituents were isolated and repeatedly purified on silica gel column chromatography, and were identified on the basis of physicochemical and spectroscopic analysis. The neurotrophic activity of different portion and all purified compounds from I. arguta was determined on the model of PC-12 cell.</p><p><b>RESULT</b>Five compounds were isolated from BuOH portion of alcohol extraction of I. arguta. Their structures were identified as plantarenaloside (I), 5-hydroxy-4', 6 7-trimethoxy-flavone (II), 4', 5-dihydroxy-6, 7-dimethoxyflavone (III), 4', 5-dihydroxy-7-methoxyflavone (IV), 5-dydroxy-4', 7-dimethoxyflavone (V).</p><p><b>CONCLUSION</b>Compound I is isolated from the plant for the first time and it has neurotrophic activity for PC-12 cell. Compounds II approximately V are isolated from the genus Incarvillea for the first time.</p>