ABSTRACT
Murine bone marrow cells were cryopreserved in liquid nitrogen with programmable freezing system as a source of hematopoietic cells to transplant into irradiated recipients for the treatment of hematopoietic failure. The results showed that CFU-GM in graft was slightly affected by freezing with a recovery of 75.7%, but CFU-S which act as major role in hematopoietic recons-titution was not obviously injuried (recovery of 126.7%). In the transplantation study, the frozen-thawed bone marrow cells gained survival rate of 43.6% which was slightly higher than obtained by fresh cells, suggesting that cryopreserved bone marrow cells still have intact hematopoietic reconstructive ability. For the purpose of preventing graft-versus-host disease (GVHD), chalone, an extract from calf spleen, was used in our studies and inhibited the early GVHD effectively.
ABSTRACT
Lymphocyte chalone was partially purified by ultrafiltration and chromatography on Sephadex G75. Its inhibitory effect on proliferation of T, B lymphocyte was demonstrated by [3H]-TdR incorporation method. In mouse bone marrow transplantation model, the treatment with lymphocyte chalone in vivo and in vitro was found to significantly improve the 30 d survival rate and prolong mean survival time, suggesting that it has an inhibitory effect on acute GVHD and is beneficial to H-2 incompatible bone marrow transplantation in mice.