Prevalence of Echinococcus infection in small mammals captured from Shiqu County, Sichuan Province from 2015 to 2020 / 中国血吸虫病防治杂志

Objective To investigate the prevalence of Echinococcus infection in small mammals in Shiqu County, Sichuan Province from 2015 to 2020, so as to provide insights into echinococcosis control in Shiqu County. Methods One setting with frequent activity of small mammals was sampled as the survey site from each of 9 townships where human alveolar echinococcosis was hyperendemic, in Shiqu County, Sichuan Province from 2015 to 2020. Two quadrats measuring 50 m × 50 m were assigned in each survey site during the period between July and August from 2015 to 2020 to capture all small mammals in quadrats, and the species of small mammals were identified by morphological characteristics. All captured small mammals were dissected in the field and Echinococcus infection was identified by visual examinations. The affected organs of Echinococcus-infected small mammals were collected, and Echinococcus infection was detected using PCR assay, with Echinococcus species characterized. The prevalence of Echinococcus infection was calculated in small mammals, and the trends in the prevalence of Echinococcus infection were analyzed during the period from 2015 to 2020. In addition, the prevalence of Echinococcus infection was compared in small mammals using visual examinations and PCR assay. Results A total of 2 692 small mammals were captured in the survey sites of Shiqu County from 2015 to 2020, and morphology characterized 1 360 Microtus fuscus (50.52%) and 1 332 Plateau pika (49.48%). The prevalence rates of Echinococcus infection were 35.63%, 19.16%, 21.41%, 8.40%, 7.68% and 4.44% by visual examinations and 18.96%, 5.36%, 5.61%, 4.58%, 3.30% and 0.37% by PCR assay in small mammals in Shiqu County from 2015 to 2020, both showing a tendency towards a decline year by year (χ2 = 215.024 and 117.045, both P values < 0.001). The prevalence of Echinococcus infection was significantly higher in small mammals by visual examinations than by PCR assay during the period from 2015 to 2020 except in 2018 (χ2= 33.597, 21.815, 51.373, 17.268 and 9.537, all P values < 0.01). PCR assay detected a reduction in the prevalence of E. multilocularis infection from 10.21% to 0.37% and a reduction in the prevalence of E. shiquicus infection from 8.75% to 0 in small mammals in Shiqu County from 2015 to 2020, both appearing a tendency towards a decline year by year (χ2 = 117.045 and 43.436, both P values < 0.001). In addition, the prevalence of E. multilocularis and E. shiquicus infections reduced from 15.19% to 0.45% and from 8.23% to 0 in M. fuscus, and the prevalence of E. multilocularis and E. shiquicus infections reduced from 7.76% to 0 and from 9.01% to 0 in P. pika in Shiqu County from 2015 to 2020. Conclusions M. fuscus and P. pika were dominant species of small mammals in Shiqu County, Sichuan Province from 2015 to 2020, and E. multilocularis infection was mainly found in M. fuscus and E. shiquicus infection mainly found in P. pika. The prevalence of Echinococcus infection appeared a tendency towards a decline in both M. fuscus and P. pika year by year during the period from 2015 to 2020.

Correlation between waist circumference and psychological sub health of middle school students in Xinjiang / 中国学校卫生

Objective@#To understand the association between waist circumference with psychological sub health among middle school students in Xinjiang, and to provide evidence for the management of waist circumference and effective prevention of mental health problems.@*Methods@#By using the stratified cluster sampling, 6 191 middle school students aged 13-15 in Urumqi, Karamay, Yining, and Kashgar of Xinjiang were surveyed on demographic information, lifestyle, waist circumference, and psychological sub health symptoms. Chi square test, one way ANOVA, and Logistic regression analysis were used to analyze the association between waist circumference and mental sub health.@*Results@#The detection rate of psychological subhealth among secondary school students in Xinjiang was 21.4%. The detection rates of emotional problems, behavioral problems, and social adjustment difficulties were 28.4 %, 28.1%, and 17.7%, respectively. Significant differences in the detection rate of emotional problems, social adjustment difficulties, and psychological sub health scores were observed among students with different waist circumference levels ( F= 4.03 , 10.90 , 5.81, P <0.05). Logistic regression analysis showed that secondary school students with high waist circumference had a higher risk of social adjustment difficulties ( OR=1.45, 95%CI =1.20-1.75) and psychological sub health problems ( OR=1.46, 95%CI =1.22-1.74) compared to those with normal waist circumference ( P <0.01).@*Conclusion@#Psychological sub health problems of middle school students in Xinjiang are prominent, and the waist circumference is positively related to the psychological sub health problems. Schools should pay attention to the mental health education of middle school students, ensure reasonable diet, sleep and living habits, and promote their physical and mental health development.

Study on the correlation between retinal microvascular density and damage to visual field in patients with sellar region tumor / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To evaluate the changes of retinal microvascular density in patients with sellar region tumor, and its correlation with the damage to visual field, and to explore its application value in evaluating optic nerve injury of those patients.METHODS: Cross-sectional study. A total of 157 patients(292 eyes)with sellar region tumor, including 82 cases(152 eyes)of pituitary adenoma and 75 cases(140 eyes)of craniopharyngioma, were selected from neurosurgery department and ophthalmology department of Beijing Tiantan Hospital, Capital Medical University between October 2018 and May 2022. A total of 90 people(180 eyes)during the same period, including the family members of patients, students and staff in Beijing Tiantan Hospital, Capital Medical University were collected as control group. All participants underwent optical coherence tomography angiography(OCTA)examination. The changes of retinal microvascular density and its correlation with visual field parameters were compared between the two groups.RESULTS: In patients with sellar region tumor, the radial peripapillary capillary(RPC)and superficial retinal capillary plexus(SRCP)density were significantly lower than that in the control group [50.81%(46.49%, 53.49%)vs. 52.78%(50.73%, 54.51%)and 50.57%(48.13%, 52.73%)vs. 51.63%(49.78%, 53.02%), all P<0.05]. The RPC density in the craniopharyngioma group was lower than that in the pituitary adenoma group [49.71%(44.33%, 53.14%)vs. 51.37%(47.42%, 53.95%), P<0.05]. The MD, PSD and VFI of the sellar region tumor group were -4.33(-12.22, -1.85)dB, 3.37(1.91, 8.82)dB and 92%(65%, 97%)respectively. RPC density of patients with sellar region tumor was positively correlated with MD and VFI, and was negatively correlated with PSD. The SRCP density of each quadrant was positively correlated with MD, and was positively correlated with VFI except Para-T and it was negatively correlated with PSD(all P<0.05).CONCLUSION: Retinal microvascular changes were present in patients with sellar region tumor. Lower vessel density indicates more severe damage to visual field. In the clinic, visual field examinations combined with OCTA were helpful to find the optic nerve injury of patients.

Optimization of the preparation process of ethanol extracts from Centipeda minima and study on its anti-inflammatory activity of different extraction sites / 中国药房

OBJECTIVE To optimize the preparation technology of ethanol extracts from Centipeda minima， and investigate the anti-inflammatory activities of different extraction sites. METHODS Single factor test and response surface methodology were adopted to investigate the effects of ethanol volume fraction， extraction time， solid-liquid ratio and extraction times on the heating reflux extraction technology of total triterpenoids ethanol extract using the extraction rate of total triterpenoids of C. minima as indexes， optimize the extraction technology and carry out validation. Using dexamethasone as positive control drug， the effects of different extraction sites of C. minima （petroleum ether part， ethyl acetate part， n-butanol part， water part） on nitric oxide （NO） production in mononuclear macrophage RAW 264.7 cells of mice induced by lipopolysaccharide （LPS） were compared； the half inhibitory concentration （IC50） was calculated. RESULTS The optimal extraction technology of total triterpenoids ethanol extracts of C. minima was as follows： ethanol volume fraction of 70%， solid-liquid ratio of 1∶40 （g/mL）， extraction time of 2.0 h and extraction times of 3 times. The 3 times of validation tests showed that average extraction rate of total triterpenoids of C. minima was 1.134%， relative error of which with the predicted value was 0.02%. The petroleum ether part and ethyl acetate part of C. minima could inhibit the generation of NO in RAW 264.7 cells induced by lipopolysaccharide to different degrees. IC50 values of NO production were 2.44 μg/mL and 2.22 μg/mL， respectively， and both of them were lower than those of positive control drug dexamethasone （7.65 μg/mL）. CONCLUSIONS The optimized preparation process of ethanol extracts from C. minima is stability and feasibility. The petroleum ether part and ethyl acetate part have obvious anti-inflammatory effects.

Natural reversal of mandibular condylar cartilage histological damage caused by chronic unpredictable moderate stress in rats / 口腔疾病防治

Objective @#To investigate the histological damage recovery of temporomandibular joint condylar cartilage caused by chronic unpredictable moderate stress, aiming to provide an experimental basis for the prevention and treatment of temporomandibular disorder.@*Methods @#This animal experiment was approved by the Laboratory Animal Ethical Inspection, School of Stomatology, The Fourth Military Medical University (No. 2020081). 60 male SD rats were randomly divided into control group, stress group, and 2-, 4- and 8-week post-stress recovery groups. Rats were subjected to chronic unpredictable moderate stress (CUMS) for 8 weeks including damp sawdust for 24 hours, tilted cage for 12 hours, noise for 4 hours, light/dark cycle reversal, water immersion, tail clamp, and restraint stress. The serum assessment, behavioral tests, histological and ultrastructural observation were performed 2-, 4- and 8-weeks after stress factors were removed. Serum levels of adrenocorticotropic hormone (ACTH) and corticosterone (CORT) were determined with ELISA. The sucrose preference test (SPT) and the forced swim test (FST) were used to assess the depressive-like behavior. The expression level of interleukin-1α (IL-1α) and matrix metalloproteinases-3 (MMP-3) were determined by Immunohistochemistry and Western blot.@*Results @#At the end of 8 weeks of CUMS, the serum levels of CORT and ACTH were significantly higher in stress group compared with control group (P<0.01). The sucrose preference decreased significantly and the immobility time increased significantly in the stressed rats compared with those in the control group, indicating a successful establishment of CUMS. The condylar cartilage showed significant degenerative changes, with disorganized collagen fibers and reduced proteoglycan synthesis on the cartilage surface. IL-1α and MMP-3 were expressed in the intracellular and extracellular matrix of the condylar cartilage, and their expression levels were increased (P<0.01). After 2 weeks of stress removal, the serum levels of CORT and ACTH were decreased but higher than control group (P<0.01), and behavioral changes were still different from the control group (P<0.01); the loosened collagen fibers could still be seen on the surface of condylar cartilage, and some free cell areas were visible within the proliferative layer; additionally, IL-1α and MMP-3 expression in the condyle was reduced in all layers of cartilage when compared with the stress group, but was still higher than in the control group (P<0.01). After 4 weeks of stress removal, the serum levels of CORT and ACTH changes returned to normal levels and behavioral changes were still different from control group (P<0.05); a few collagen fibers could be seen on the surface of the condylar cartilage and the expressions of IL-1α and MMP-3 decreased significantly compared with the stress group (P<0.01), with the similar level of IL-1α (P>0.05) and higher expression of MMP-3 comparing with the control group (P<0.01). After 8 weeks of stress removal, behavioral changes returned to normal levels, with no statistically significant differences compared with the control group (P>0.05). The condylar collagen fibers increased and showed a corrugated pattern, and no serious subchondral bone damage as well as irreversible damage occurred. Both of the expression levels of IL-1α and MMP-3 approached those of the control group after 8 weeks of stress removal (P>0.05). @*Conclusion@# The behavioral changes and condylar cartilage damage caused by CUMS could be self-repaired. The decline in IL-1α and MMP-3 expression may be one of the intrinsic mechanisms of this self-repair process.

Situation and prevention strategies of sports injuries in community home sports under the background of covid-19 / A situação e estratégias de prevenção de lesões esportivas em esportes domésticos comunitários sob o pano de fundo da covid-19 / La situación y las estrategias de prevención y tratamiento de las lesiones deportivas en el hogar en la comunidad en el contexto del covid - 19

ABSTRACT Introduction: In the context of COVID-19, many physical activities have been restricted to the home environment, including traditional community sports. The situation of bodily injuries and prevention strategies during this period urges investigation and adaptation for a preventive approach. Objective: Explore the current scenario by devising means of prevention for sports injuries caused in the home environment. Methods: A total of 60 people were selected as experimental volunteers for home sports injury analysis, 30 in the experimental group and 30 in the control group, the cases were studied, and hypotheses were raised corroborating a preventive system of the cataloged home sports injuries, as well as the mitigation of their intercurrences. Results: In the experimental group, the flexion amplitude increased from 74.55±5.30 to 95.19±6.54; the extension amplitude increased from −3.88±3.29 to 1.57±2.17; the walking index evolved from 22.61±7.00 to 28.53±5.74; the stair activity index increased from 18.70±6.79 to 18.70±6.79. The same data measured in the control group showed no significant changes. Conclusion: Prevention of sports injuries during the current pandemic begins with the care of the immune system; care of the home environment was also described, as well as validation of effective treatment for timely rehabilitation of home sports injuries. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: No contexto da COVID-19, muitas atividades físicas restringiram-se ao ambiente doméstico, incluindo os esportes tradicionalmente comunitários. A situação das lesões corporais e as estratégias de prevenção durante este período urgem investigações e adaptações para uma abordagem preventiva. Objetivo: Explorar o cenário atual elaborando meios de prevenção para as lesões esportivas ocasionadas em ambiente domiciliar. Métodos: Um total de 60 pessoas foram selecionadas como voluntários experimentais para análise de lesão esportiva domiciliar, 30 no grupo experimental e 30 no grupo de controle, estudou-se os casos e foram levantadas hipóteses corroborando para um sistema preventivo das lesões esportivas domiciliares catalogadas, bem como a atenuação de suas intercorrências. Resultados: No grupo experimental, a amplitude de flexão aumentou de 74,55±5,30 para 95,19±6,54; a amplitude de extensão aumentou de −3,88±3,29 para 1,57±2,17; o índice de caminhada evoluiu de 22,61±7,00 para 28,53±5,74; o índice de atividade em escadas aumentou de 18,70±6,79 para 18,70±6,79. Os mesmos dados aferidos no grupo controle não apresentaram alterações significativas. Conclusão: A prevenção de lesões esportivas durante a pandemia atual inicia-se no cuidado do sistema imunológico, cuidados com o ambiente doméstico também foram descritos, bem como a validação de tratamento eficaz para a reabilitação das lesões esportivas domésticas em tempo hábil. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: En el contexto del COVID-19, muchas actividades físicas se han restringido al ámbito doméstico, incluidos los deportes comunitarios tradicionales. La situación de las lesiones corporales y las estrategias de prevención durante este período urgen investigaciones y adaptaciones para un abordaje preventivo. Objetivo: Explorar el escenario actual ideando medios de prevención para las lesiones deportivas causadas en el entorno doméstico. Métodos: Un total de 60 personas fueron seleccionadas como voluntarios experimentales para el análisis de las lesiones deportivas domiciliarias, 30 en el grupo experimental y 30 en el grupo control, se estudiaron los casos y se plantearon hipótesis corroborando para un sistema preventivo de las lesiones deportivas domiciliarias catalogadas, así como la mitigación de sus intercurrencias. Resultados: En el grupo experimental, la amplitud de flexión aumentó de 74,55±5,30 a 95,19±6,54; la amplitud de extensión aumentó de −3,88±3,29 a 1,57±2,17; el índice de marcha evolucionó de 22,61±7,00 a 28,53±5,74; el índice de actividad en escaleras aumentó de 18,70±6,79 a 18,70±6,79. Los mismos datos medidos en el grupo de control no mostraron cambios significativos. Conclusión: La prevención de las lesiones deportivas durante la pandemia actual comienza en el cuidado del sistema inmunológico, también se describió el cuidado del entorno del hogar, así como la validación de un tratamiento eficaz para la rehabilitación de las lesiones deportivas en el hogar de manera oportuna. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Impacts of physical coordination training on speed in short track speed skating / Impactos do treinamento de coordenação física sobre a velocidade na patinação de velocidade em pista curta / Efectos del entrenamiento de la coordinación física sobre la velocidad en el patinaje de velocidad en pista corta

ABSTRACT Introduction Although the development time of short-track speed skating in China is relatively short, with the joint efforts of many athletes and coaches, the special technical level can be comparable with that of European and American countries. Objective Verify the impacts of physical coordination training on speed in short track speed skating. Methods Athletes from the provincial skating team were randomly selected and distributed into control and experimental groups to apply the training method on physical coordination in short track speed skating. Results The average body fat rate of the skaters in the control group was 15.24%, the average body fat rate in the control group was 15.20%, and the T value of the experimental group and control group was 1.00, P = 0.374 < 0.05. Conclusion Because they are special equipment, the turn resistance training device and the air resistance parachute played an important role in improving the special fitness of Chinese short track speed skaters. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução Embora o tempo de desenvolvimento da patinação em pista curta na China seja relativamente curto, com os esforços conjuntos de muitos atletas e treinadores, o nível técnico especial pode ser comparável com o dos países europeus e americanos. Objetivo Verificar os impactos do treinamento de coordenação física sobre a velocidade na patinação de velocidade em pista curta. Métodos Atletas da equipe de patinação provincial foram aleatoriamente selecionados e distribuídos em grupos controle e experimental para aplicar o método de treinamento na coordenação física de patinação de velocidade em pista curta. Resultados A taxa média de gordura corporal dos patinadores no grupo controle foi 15,24%, a taxa média de gordura corporal no grupo de controle foi 15,20%, e o valor de T do grupo experimental e do grupo de controle foi 1,00, P = 0,374 < 0,05. Conclusão Por serem equipamentos especiais, o dispositivo de treinamento de resistência à curva e o paraquedas de resistência ao ar desempenharam um papel importante na melhoria da aptidão física especial dos patinadores chineses de velocidade em pista curta. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción Aunque el tiempo de desarrollo del patinaje de velocidad en pista corta en China es relativamente corto, con el esfuerzo conjunto de muchos atletas y entrenadores, el nivel técnico especial puede ser comparable al de los países europeos y americanos. Objetivo Verificar los impactos del entrenamiento de la coordinación física sobre la velocidad en el patinaje de velocidad en pista corta. Métodos Se seleccionaron aleatoriamente atletas del equipo provincial de patinaje y se distribuyeron en grupos de control y experimentales para aplicar el método de entrenamiento de la coordinación física en el patinaje de velocidad en pista corta. Resultados El índice medio de grasa corporal de los patinadores del grupo de control fue del 15,24%, el índice medio de grasa corporal del grupo de control fue del 15,20%, y el valor T del grupo experimental y del grupo de control fue de 1,00, P = 0,374 < 0,05. Conclusión Debido a que son equipos especiales, el dispositivo de entrenamiento de resistencia en curva y el paracaídas de resistencia al aire desempeñaron un papel importante en la mejora de la aptitud física especial de los patinadores de velocidad de pista corta chinos. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Improving explosive body capacity in female short track speed skaters / Melhoria da capacidade explosiva corporal em patinadoras de velocidade em pista curta / Mejora de la capacidad explosiva corporal en patinadoras de velocidad en pista corta

ABSTRACT Introduction The peculiar characteristics of short track speed skating should be integrated into the psychology of competitions; it is considered that elite athletes engaged in this particular sport should have a healthy psychic condition. Objective Investigate the explosive power of female speed skaters in short track speed skating. Methods 10 key athletes from the national short track speed skating team were selected, and explosive power was tested by T-test, hexagonal test, and pro sensitivity test. Data analysis was performed using an independent sample t-test, differences in the results of related test indicators between groups were analyzed, and repeated measures analysis of variance was used. Results During the explosive kick phase, knee extension speed increased linearly from 210°/S to 600°/S, and hip extension speed increased linearly from 200°/S to 400°/S. Conclusion The development of muscle group strength and explosive power during training often shows differences in the degree of contraction, which is related to the arrangement of training methods. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução As características peculiares da patinação de velocidade em pista curta devem ser integradas na psicologia das competições; considera-se que os atletas de elite engajados nesta modalidade especial devem ter boa condição psíquica. Objetivo Investigar o poder explosivo das patinadoras de patinação de velocidade em pista curta. Métodos 10 atletas-chave da equipe nacional de patinação de velocidade em pista curta foram selecionadas, o poder explosivo foi testado por teste T, teste hexagonal e teste de sensibilidade pro. A análise dos dados foi realizada utilizando um teste t de amostra independente, as diferenças nos resultados dos indicadores de teste relacionados entre os grupos foram analisadas, e foi utilizada a análise de medidas repetidas de variação. Resultados Durante a fase de pontapé explosivo, a velocidade da extensão do joelho aumentou linearmente de 210°/S para 600°/S, e a velocidade da extensão do quadril aumentou linearmente de 200°/S para 400°/S. Conclusão O desenvolvimento da força do grupo muscular e do poder explosivo durante o treinamento muitas vezes apresenta diferenças no grau de contração, o que está relacionado com a disposição dos métodos de treinamento. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción Las características peculiares del patinaje de velocidad en pista corta deben integrarse en la psicología de las competiciones; se considera que los atletas de élite que se dedican a esta modalidad especial deben tener una buena condición psíquica. Objetivo Investigar la potencia explosiva de las patinadoras de velocidad en pista corta. Métodos Se seleccionaron 10 atletas clave del equipo nacional de patinaje de velocidad en pista corta, se probó la potencia explosiva mediante la prueba T, la prueba hexagonal y la prueba de sensibilidad pro. El análisis de los datos se realizó mediante una prueba t de muestras independientes, se analizaron las diferencias en los resultados de los indicadores de las pruebas relacionadas entre los grupos y se utilizó el análisis de varianza de medidas repetidas. Resultados Durante la fase de patada explosiva, la velocidad de extensión de la rodilla aumentó linealmente de 210°/S a 600°/S, y la velocidad de extensión de la cadera aumentó linealmente de 200°/S a 400°/S. Conclusión El desarrollo de la fuerza y la potencia explosiva de los grupos musculares durante el entrenamiento suele mostrar diferencias en el grado de contracción, lo que está relacionado con la disposición de los métodos de entrenamiento. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways

Abstract Objective Abnormal complement activation is associated with periodontitis. W54011 is a novel non-peptide C5aR antagonist (C5aRA) that exhibits favorable anti-inflammatory effects in various inflammatory models. However, whether W54011 inhibits periodontitis has not yet been fully elucidated. To address this, we have investigated the probable anti-inflammatory mechanism of W54011 in LPS-treated inflammation in human gingival fibroblasts (HGFs). Methodology HGFs were isolated from healthy gingival tissue samples using the tissue block method and were identified with immunofluorescence staining. The CCK8 assay and reverse transcription-PCR (RT-PCR) were used to select the optimal induction conditions for Lipopolysaccharide (LPS) and C5aRA (according to supplementary data S1, S2 and S3). The levels of inflammatory cytokines, C5aR, and the activation of NF-κB/MAPK signaling pathways were determined by RT-quantitative PCR (RT-qPCR) and Western blotting. Results Immunofluorescence results showed that vimentin and FSP-1 were positive in HGFs and Keratin was negative in HGFs. Immunofluorescence staining demonstrated that C5aRA inhibited LPS-stimulated nuclear translocation of p-p65. RT-qPCR and Western blotting showed that C5aRA reduced the expression of IL-1β, IL-6, TNF-α, C5aR, p-p65, p-IκBα, p-JNK, p-c-JUN, and TLR4 in LPS-induced HGFs. Conclusion These findings suggested that C5aRA attenuated the release of inflammatory cytokines in LPS-induced HGFs by blocking the activation of the NF-κB and MAPK signaling pathways.

Long-term effects of antipsychotics on mortality in patients with schizophrenia: a systematic review and meta-analysis

Objective: To gather current evidence on the impact of antipsychotics on long-term mortality in patients with schizophrenia. Methods: We systematically searched for articles in Embase, PubMed, and PsycINFO reporting the long-term mortality (follow-up > 1 year) of patients with schizophrenia who were using any antipsychotics. We then conducted multiple meta-analyses to determine differences in long-term mortality between different types of antipsychotics. Results: We identified 45 articles that provided unadjusted long-term mortality rates, including 46,171 deaths during 2,394,911 person-years. The pooled mortality rate was 9.9 (95%CI = 7.4-12.7) per 1,000 person-years. The unadjusted crude mortality rate of antipsychotic drug users was lower than that of non-users (risk ratio [RR] = 0.546, 95%CI = 0.480-0.621), first-generation antipsychotics caused higher all-cause mortality than second-generation antipsychotics (RR = 1.485, 95%CI = 1.361-1.620), and polypharmacy had better effects than monotherapy on long-term mortality (RR = 0.796, 95%CI = 0.689-0.921). As for the causes of death, heart disease and cardiovascular disease ranked highest among cause-specific mortality (5.6 per 1,000 person-years). Conclusion: Since antipsychotics had a beneficial effect on long-term mortality in schizophrenia, greater precaution should be taken with patients who do not take them. However, since disease severity, comorbidities, and other confounding factors cannot be fully controlled, further research and verification are needed.

Meta-Analysis and Systematic Review of the Relationship between Cervical Lesions, HPV and Vaginal Community State Type

Background: Chronic HPV infection is a precursor of cervical cancer, which is largely caused by dysregulation of vaginal flora and other factors like abnormal H2O2, neuraminidase and insufficient vaginal hygiene. The relationship between HPV-induced cancer and vaginal microbiota is involved in the viral chronicity and also influences the disease prognosis. A meta-analysis system was used to evaluate the relationship between cervical lesions, HPV and vaginal microenvironment. Methods: PubMed, Web of Science, Cochrane and Embase databases were searched for relevant literature published from 2016 to December 2020. According to the inclusion and exclusion criteria, literature screening, data extraction and quality evaluation were carried out, and stata16 statistical software was used for Meta-analysis and systematic evaluation. Results: The overall relative risk of CST in 95% CI: 0.76-1.4, LSIL group compared with normal cytology group was 0.81. The overall relative risk of CST in the HSIL group and cervical cancer group was 0.77 and 1.26, respectively. It was found that there was publication bias in the HPV positive group (p-value of Begg and Egger were 0.067 and 0.247) and cervical cancer group (p-value of Begg and Egger were 0.677 and 0.457 respectively). There was a significant difference in CST III between HPV positive group and the LSIL group. Conclusion: Cervical lesions and HPV are related to the increase of vaginal microbial diversity, and HPV and LSIL groups are related to CST III, while HSIL and cervical cancer groups are related to CSTIV, which has a certain guiding significance for early clinical diagnosis, but further large-scale studies are needed to confirm our findings.

Effects of Buyang Huanwu Decoction on renal oxidative stress and NF-κB pathway in diabetic nephropathy rats / 国际中医中药杂志

Objective:To observe the effect of Buyang Huanwu Decoction on renal oxidative stress and NF-κB pathway in diabetic nephropathy rats.Methods:Sixty SD rats were randomly divided into normal group, model group, low, medium, and high dose of Buyang Huanwu Decoction groups and metformin group, with 10 rats in each group. The normal group was given ordinary diet every day, and the other groups were given high-fat and high-sugar diet for 6 weeks, then intraperitoneally injected 35 mg/kg STZ to establish diabetic nephropathy model. After successful modeling, the rats were orally injected with 7.42, 14.84 and 29.68 g/kg of low-dose, medium-dose and high-dose Buyang Huanwu Decoction and metformin group was orally given metformin solution 0.2 g/kg. After 6 weeks of feeding, the body weight, 24h urinary protein, serum creatinine and urea nitrogen were detected. The levels of GSH-Px, SOD, CAT and MDA in rats' kidney of each group were detected. The protein expression levels of NF-κBp65, p-NF-κBp65, TNF-α and IL-6 in rats' kidney in each group were detected by Western Blot.Results:Compared with model group, the body weight of the low, medium and high dose of Buyang Huanwu Decoction groups were increased ( P<0.01), the content of 24 h urinary protein, serum creatinine and urea nitrogen were decreased ( P<0.05 or P<0.01),the content of GSH-Px , SOD, CAT were significantly increased ( P<0.05 or P<0.01), the content of MDA was significantly decreased ( P<0.05 or P<0.01), the expression of NF-κB p65 (0.53±0.07, 0.46±0.09, 0.42±0.10 vs. 0.67±0.13), p-NF-κB p65 (0.51±0.12, 0.43±0.06, 0.34±0.17 vs. 0.59±0.07), TNF-α (1.21±0.08, 1.17±0.04, 1.05±0.22 vs. 1.43±0.21), IL-6 (0.92±0.04, 0.89±0.25, 0.73±0.09 vs. 1.06±0.08) were significantly decreased ( P<0.05 or P<0.01). Conclusion:Buyang Huanwu Decoction can improve oxidative stress state and inflammatory damage in renal tissue. The mechanism may be related to the inhibition of NF-κB signaling pathway.

Progress of microbiota-gut-brain axis in the pathogenesis of white matter injury in preterm infants / 国际儿科学杂志

White matter injury in preterm infants is a unique form of brain injury.It is one of the most common cause of chronic neurological diseases such as cerebral palsy and various neurobehavioral disorders.Perinatal infection, inflammation and hypoxic ischemia are related to the pathophysiology of white matter injury in preterm infants.Signals released by the gut microbiota, such as microbial metabolites, can modulate inflammatory and immune responses characterized by microglial activation, and ultimately it affects the differentiation of premyelin oligodendrocytes and causes white matter injury in preterm infants.Recently more attention has been paid to the role of gut microbiota in the pathogenesis of white matter injury in preterm infants.This article reviews the progress of "microbiota-gut-brain axis" involved in the pathogenesis of white matter injury in preterm infants, so as to provide reference for further prevention and treatment of white matter injury.

Exon 35 skipping of FBN1 caused by c.4336G>A mutation may lead to Marfan syndrome / 中华检验医学杂志

Objective:To investigate the genetic etiology of a Marfan syndrome pedigree, and the impact of c.4336G>A variant on the splicing process of FBN1 gene.Methods:The proband was admitted to the Department of Cardiovascular Surgery of Xijing Hospital due to thoracic aortic aneurysm and dissection in August 2019. Multiplex PCR and next generation sequencing technology were used to detect 15 genes associated with hereditary aortic diseases in the proband. Then the pathogenic sites were further verified by Sanger sequencing, and above examinations were also performed among the family members of the proband. The effect of the mutation on mRNA splicing was predicted by splicing prediction software. RNAs from peripheral blood cells of the proband and the healthy person were extracted, and the effect of the mutation on mRNA splicing was verified by reverse transcription PCR and Sanger sequencing. The pathogenicity was analyzed by the recommendations from the American College of Medical Genetics (ACMG).Results:The gene panel detected a missense mutation of FBN1 gene (c.4336G>A) in the proband. Sanger sequencing results were consistent with that of panel. Sanger sequencing results showed that 4 family members were carriers of the same variant, and 3 out of the 4 family members presented signs of thoracic aortic aneurysm and dissection. The dbscSNV_ada_score and dbscSNV_rf_score software predicted that this mutation would lead to the occurrence of abnormal splicing of mRNA. The skipping of exon 35 was verified in the subsequent examinations by reverse transcription PCR and Sanger sequencing. The variant was classified as"pathogenic"according to ACMG guideline.Conclusion:FBN1 c.4336G>A mutation can cause the skipping of exon 35, and this might be the genetic mechanistic of severe cardiovascular abnormalities observed in this Marfan syndrome pedigree.

Hereditary haemochromatosis presenting as diabetes and complicated with abnormal liver function, hypogonadism, osteoporosis: a case report / 中华内分泌代谢杂志

Hereditary hemochromatosis(HH) is relatively rare in the Chinese population, and the disease can involve multiple systems. It is easy to be missed and misdiagnosed due to nonspecific clinical manifestations. We report on a case with diabetes as the first diagnosis and being confirmed HH later. In addition to abnormal liver function, this patient also developed a variety of endocrine and metabolic diseases such as hypogonadism and osteoporosis. Included with this case report is a literature based discussion of clinical features, management of HH along with its relationship with endocrine dysfunction to improve disease understanding.

Research progress on the role and intervention strategies of NRP1 in colorectal cancer / 药学学报

Colorectal cancer (CRC) is one of the most common malignant tumors in the world, and its incidence and mortality are among the top three of all malignant tumors. In recent years, CRC is becoming more common in younger patients. Currently, surgery is the main or first treatment of early stage CRC, however, up to 50% patients have recurrence and metastasis post-surgery. While chemotherapy and radiotherapy are often used as adjuvant treatment after surgery or as main treatment options for late stage CRC, they usually induce severe adverse effects. Safe and effective treatments for CRC are still lacking. Therefore, it is essential to discover new therapies for CRC. Neuropilin 1 (NRP1), as a transmembrane glycoprotein, is reported to highly express in CRC, and its overexpression is demonstrated to be closely related to the occurrence and development of CRC. NRP1 is involved in angiogenesis, tumor growth, autophagy, and lipid metabolism, which is expected to be a potential new target for the treatment of CRC. This paper reviews the role of NRP1 in CRC, including its molecular structure, expression in CRC, as well as its connection with autophagy and metabolism. The regulatory factors of NRP1 in CRC were introduced, including vascular endothelial growth factor (VEGF), semaphorin 3A (SEMA3A), transforming growth factor-β (TGF-β), etc. The potential intervention strategies of CRC targeting NRP1 were summarized in order to provide reference for the diagnosis and prevention of CRC.

Sedentary behavior among children and adolescents with intellectual disabilities in Jinan City / 中国学校卫生

Objective@#To understand the sedentary behavior level of children and adolescents with intellectual disabilities in Jinan City, and to provide a reference basis for developing health behavior intervention strategies.@*Methods@#By used the method of cluster random sampling,the Children s Leisure Activities Study Survey was used to investigate the sedentary behavior level of 285 children and adolescents with intellectual disabilities aged 6-18 years from 7 special education schools in Jinan City.@*Results@#The sedentary behavior time during the whole week among children and adolescents with intellectual disabilities in Jinan City was 394.46 min/d, including 378.00 min/d on weekdays(Monday to Friday) and 388.80 min/d on weekends (Saturday and Sunday), the difference was statistically significant ( Z =-2.19, P <0.05). 80.4%(229) of children and adolescents with intellectual disabilities had sedentary behavior time of more than 2 h/d. The sedentary behavior time per day during the whole week among children and adolescents with intellectual disabilities was negatively correlated with the amount of time spent in moderate vigorous physical activity among them ( r =-0.16, P <0.05).@*Conclusion@#Excessive sedentary behavior has become a growing public health concern among children and adolescents with intellectual disabilities,which warrants targeted healthy behavior intervention.

The function of auditory cortex in the elderly using functional near-infrared spectroscopy technology / 中华耳鼻咽喉头颈外科杂志

Objective: Functional near-infrared spectroscopy (fNIRS) was used to study the effect of aging on the neuroimaging characteristics of cerebral cortex in the process of speech perception. Method: Thirty-four adults with normal hearing were recruited from March 2021 to June 2021, including 17 in the young group, with 6 males, 11 females, age (32.1±5.0) years, age range 20-39 years. and 17 in the elderly group, with 6 males, 11 females, age (63.2±2.8) years, age range 60-70 years. The test material was the sentence table of the Mandarin Hearing Test in Noise (MHINT). The task state block experiment design was adopted, and the temporal lobe, Broca's area, Wernicke's area, motor cortex were used as regions of interest. Objective brain imaging technology (fNIRS) combined with subjective psychophysical testing method was used to analyze the activation area and degree of cerebral cortex related to auditory speech perception in the elderly and young people under different listening conditions (quiet, signal-to-noise ratio of 10 dB, 5 dB, 0 dB, -5 dB). SPSS 23 software was used for statistical analysis. Result: The activation area and degree of activation in the elderly group were lower than those in the young group under each task condition; The number of activation channels in the young group were significantly more than those in the old group, and the number of activation channels in the left hemisphere were more than those in the right hemisphere, but there was no difference in the number of activation channels. There were more channels affected by age in the left hemisphere than in the right hemisphere. The activation degree of the young group when the signal-to-noise ratio was 0 dB was significantly higher than that of other signal-to-noise ratio conditions (P<0.05), but there was no significant difference in the old group under the five conditions (P>0.05). The speech recognition score of the young group was higher than that of the old group under all conditions. When the quiet and signal-to-noise ratio was 10 dB, the correct score of the two groups was equal or close to 100%. With the gradual decrease of signal-to-noise ratio, there was a significant difference between the two groups when the signal-to-noise ratio was 5 dB. The speech recognition accuracy of the young group decreased significantly when the signal-to-noise ratio was 0 dB, while that of the old group decreased significantly when the signal-to-noise ratio was 5 dB. Conclusions: With the increase of age, the speech perception in noisy environment and the activity of cerebral cortex gradually deteriorate, and the speech dominance hemisphere (left hemisphere) will be significantly affected by aging. The overall activation area and activation degree of the elderly under different speech tasks are lower than those of the young.

Value of HBsAg level in predicting liver inflammation in patients with HBeAg-positive chronic hepatitis B virus infection and normal alanine aminotransferase / 临床肝胆病杂志

Objective To investigate the onset of liver inflammation and related predictive factors in patients with HBeAg-positive chronic hepatitis B virus (HBV) infection who have normal alanine aminotransferase (ALT) and a high viral load. Methods A retrospective analysis was performed for the clinical data of 183 patients with HBeAg-positive chronic HBV infection who had normal ALT and a high viral load and were treated from October 2008 to May 2015, and according to the results of liver biopsy, they were divided into hepatitis group and non- hepatitis group. The t -test or Mann-Whitney U testwas used for comparison of normally distributed continuous data between groups, the chi-square test was used for comparison of categorical data. The predictive factors were analyzed by univariate binary logistic regression, the multivariate binary logistic regression was carried out by stepback method, and the cut-off values were analyzed by receiver operating characteristic curve (ROC) and Jordan index. Results There were 37 patients (20.2%) in the hepatitis group and 146 patients (79.8%) in the non-hepatitis group. Compared with the non-hepatitis group, the hepatitis group had a significantly lower proportion of male patients (45.9% vs 68.5%, χ 2 =6.508, P =0.011), a significantly higher level of aspartate aminotransferase [24 (21.25~35.55) U/L vs 21.2 (18.08~ 24.65) U/L, Z =-3.344, P =0.001], and a significantly lower log(HBsAg) value [4.4(4.28~4.49) vs 4.46(4.4~4.74), Z =-2.184, P =0.029]. Log(HBsAg) value was a predictive factor for hepatitis (odds ratio=0.077, P =0.017), and the cutoff value of HBsAg was 33884.4I U/mL. Conclusion Among the patients with HBeAg-positive chronic HBV infection who have normal ALT and a high viral load, 20.2% have liver inflammation, and HBsAg may be a predictive factor for liver inflammation.

Association between parents health literacy and health risk behaviors of middle school students in northeast Jiangxi Province / 中国学校卫生

Objective@#To explore the association between parents health literacy and health risk behaviors of middle school students, so as to provide theoretical basis for parental health literacy improvement and adolescent health risk behaviors prevention.@*Methods@#A questionnaire survey was conducted among 1 993 middle school students and their parents in northeast Jiangxi Province by multistage stratified random sampling during Jun. to Sept. of 2020. Spearman correlation and generalized linear model were used to explore association between parents health literacy with 5 types of unhealthy behaviors.@*Results@#Generalized linear model analysis showed that after adjusting household economic status and other covariates, parents health literacy was associated with adolescent health risk behaviors, and the scientific health view ( β =-0.05, 95% CI =-0.09--0.01), health information literacy ( β = -0.06 , 95% CI =-0.08--0.05) were associated with substance abuse and violence among middle school students; health information literacy ( β =-0.03, 95% CI =-0.04--0.01) was associated with adolescent healthy eating behavior; health information literacy ( β =-0.03, 95% CI =-0.05--0.02) was associated with breakfast and exercise in middle school students; chronic disease literacy ( β =0.05, 95% CI =0.00-0.09), health information literacy ( β =-0.02, 95% CI =-0.03--0.00) was associated with screen related behavior of middle school students; scientific health view ( β =-0.05, 95% CI =-0.09--0.01), basic medical literacy ( β =-0.08, 95% CI =-0.13--0.04), health information literacy ( β =0.02, 95% CI =0.00-0.03) were associated with middle school students traffic safety behavior( P <0.05). @*Conclusion@#There is a certain cross sectional correlation between parents health literacy and adolescent health risk behaviors. It is necessary to clarify the direction and intensity of the correlation in the cohort study.