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1.
Journal of Peking University(Health Sciences) ; (6): 236-243, 2022.
Article in Chinese | WPRIM | ID: wpr-936140

ABSTRACT

OBJECTIVE@#To investigate the effect of 275 nm and 310 nm ultraviolet irradiation on ovariectomized rats' bone metabolism.@*METHODS@#Twenty four 3-month-old female Sprague-Dawley (SD) rat were randomly divided into control group, sham operated group, 275 nm ultraviolet (UV) irradiation group and 310 nm UV irradiation group. Each group contained 6 rats. The rats in the two irradiation groups were treated with bilateral ovariectomy. The rats in sham operated group received sham operation (They were given the same back incision and a bit of par-ovarian fat were removed). Control group received no disposition. About 24 weeks after operation, all the rats received detailed bone mineral density (BMD) detection again. Detection regions include cervical vertebra, lumbar vertebra, proximal femur, mid femur and distal femur. Next, osteopenia rats in 275 nm irradiation group were UV irradiated 275 nm with fixed illumination intensity (15 μW/cm2) everyday for 16 weeks. The osteopenia rats in 310 nm irradiation group were UV irradiated 310 nm with fixed illumination intensity (15 μW/cm2) everyday for 16 weeks. The backs of the rats were shaved regularly as irradiation area (6 cm×8 cm). After 16-week irradiation, all the rats' BMD of cervical vertebra, lumbar vertebra, proximal femur, mid femur and distal femur were measured. At the end of the trial, all the rats' blood specimens were obtained and serum 25(OH)D, procollagen type Ⅰ N-peptide (PINP) and osteocalcin (OC) were measured.@*RESULTS@#Compared with control group [(238.78±26.74) mg/cm3], the BMD of the whole body were significantly lower in 275 nm [(193.34±13.28) mg/cm3] and 310 nm [(191.19±18.48) mg/cm3] irradiation groups (P=0.002, P=0.001). There were no significant difference between sham operated group [(227.20±14.32) mg/cm3] and control group. After 16-week ultraviolet irradiation, the BMD of the whole body were significantly increased in 275 nm [(193.34±13.28) mg/cm3 vs. (221.68±25.52) mg/cm3, P=0.005] and 310 nm groups [(191.19±18.48) mg/cm3 vs. (267.48±20.54) mg/cm3, P < 0.001] after corresponding irradiation. The BMD of the four body regions (lumbar vertebra, proximal femur, mid femur and distal femur) had significantly increased after irradiation in 275 nm irradiation group. For 310 nm irradiation group, the BMD in cervical vertebra, lumbar vertebra, proximal femur, mid femur and distal femur also had increased significantly after 310 nm ultraviolet irradiation. The concentration of serum 25(OH)D and OC was higher in 275 nm irradiation group than in control group [(46.78±5.59) μg/L vs. (21.32±6.65) μg/L, P=0.002;(2.05±0.53) U/L vs. (1.32±0.07) U/L, P=0.022]. Compared with the control, the concentration of serum 25(OH)D [(58.05±12.74) μg/L], OC [(2.04±0.53) U/L] and PINP [(176.16±24.18) U/L] was significantly higher (P < 0.001, P=0.015, P=0.005) in 310 nm irradiation group. However, there were no significantly difference between sham operated group and the control.@*CONCLUSION@#Both 275 nm and 310 nm ultraviolet could improve rats' vitamin D synthesis. Both 275 nm and 310 nm ultraviolet could improve osteopenia rats' bone condition. The irradiation of 310 nm might be more effective on bone condition improvement.


Subject(s)
Animals , Female , Humans , Rats , Bone Density , Bone Diseases, Metabolic/metabolism , Femur/metabolism , Osteocalcin/metabolism , Ovariectomy , Rats, Sprague-Dawley
2.
Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 311-315, 2022.
Article in Chinese | WPRIM | ID: wpr-935801

ABSTRACT

Work-related musculoskeletal disorders (WMSDs) refer to musculoskeletal disorders caused by work or work as the main cause, which are characterized by high prevalence and heavy burden of disease as a global problem. The classification and catalog of occupational diseases is of great significance for guiding the prevention and control of occupational diseases and safeguarding the rights and interests of workers. The types of WMSDs included in the list of occupational diseases vary greatly from country to country, and the regulations on specific pathogenic factors are also inconsistent. By sorting out and analyzing the lists and characteristics of WMSDs at home and abroad, and using the International Statistical Classification of Diseases and Related Health Problems (ICD-10) in occupational health to standardize of WMSDs in various countries, which would lay the foundation for future multi-country WMSDs occupational health registration and disease burden research, and provide a reference for China to revise the WMSDs list.


Subject(s)
Humans , Musculoskeletal Diseases/prevention & control , Occupational Diseases/prevention & control , Prevalence , Risk Factors , Surveys and Questionnaires
3.
Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 24-27, 2022.
Article in Chinese | WPRIM | ID: wpr-935736

ABSTRACT

Objective: To investigate the effect of pesticides and herbicides on lipid metabolism. Methods: In November 2020, Based on the data of the national health and Nutrition Survey (NHANES) (2011-2014) , select the population aged 20~65 who have demographic information, pesticide use and data of four lipid metabolism indicators [total cholesterol (TC) , triglyceride (TG) , high density lipoprotein cholesterol (HDLC) and low density lipoprotein cholesterol (LDLC) ] (n=3039) . The subjects were divided into insecticide group (320 people) and non insecticide group (2719) according to the use of insecticides, and herbicide group (156 people) and non herbicide group according to the use of herbicides. Results: Among the 3039 subjects, the males and female were 1509 (49.7%) and 1530 (50.3%) respectively. The males age was (39.7±12.0) years and the females age was (40.2±12.0) years The concentration of HDLC in the NHANES (55.4±15.0) mg/dl was lower than that of (58.2±14.2) mg/dL in the non herbicide group (P<0.05) (b=-0.044, P<0.05) . The results showed that the use of herbicides was related to the decrease of HDLC and the increase of LDLC and LDLC/HDLC in female population (b=-0.050, 0.062, 0.067, all P<0.05) . Conclusion: Herbicide exposure can cause the change of lipid metabolism, and the effect on female population is more obvious.


Subject(s)
Adult , Female , Humans , Male , Middle Aged , Young Adult , Cholesterol, HDL , Cholesterol, LDL , Lipid Metabolism , Nutrition Surveys , Pesticides
4.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 95-100, 2021.
Article in Chinese | WPRIM | ID: wpr-906086

ABSTRACT

Objective:To investigate the effect of licochalcone A (LCA) on apoptosis in human breast cancer MDA-MB-231 cells, and to explore its possible mechanism. Method:MDA-MB-231 cells were treated with LCA of different concentrations, and<italic> </italic>cell counting kit-8 (CCK-8) assay was used to detect the cell viability. The cells were treated with LCA (10, 20, and 40 μmol·L<sup>-1</sup>) for 24 h, and apoptosis was detected by Annexin V staining with fluorescein isothiocyanate (FITC) and propidium iodide (PI) (Annexin V-FITC/PI). The level of intracellular reactive oxygen species (ROS) was detected by 2′,7′-dichlorodihydrofluorescein diacetate (DCFA-DA) fluorescent probe. Mitochondrial membrane potential (MMP) was detected by 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethyl-imidacarbocyanine (JC-1) fluorescence probe. Western blot was used to detect the expression of cell apoptosis-related proteins, such as B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax), and endoplasmic reticulum (ER) stress-related proteins, such as C/EBP homologous protein (CHOP), activating transcription factor 4 (ATF4), protein kinase R-like ER kinase (PERK), p-PERK, eukaryotic translation initiation factor 2 alpha (eIF2<italic>α</italic>), and p-eIF2<italic>α</italic>. Result:With the increase in the drug concentration (starting from 5 μmol·L<sup>-1</sup>), the cell viability decreased (<italic>P<</italic>0.05) with IC<sub>50 </sub>of 19.05 μmol·L<sup>-1</sup> as compared with the normal group. Additionally, the apoptosis rates of the LCA groups (10, 20, 40 μmol·L<sup>-1</sup>) significantly increased (<italic>P</italic><0.05), which reached 30.2% (<italic>P</italic><0.05) at LCA concentration of 40 μmol·L<sup>-1</sup>. LCA (10, 20, and 40 μmol·L<sup>-1</sup>) decreased the expression of Bcl-2 (<italic>P<</italic>0.05) and increased Bax expression (<italic>P<</italic>0.05) in a dose-dependent manner. Besides, the intracellular ROS level was elevated (<italic>P<</italic>0.05) and mitochondrial MMP was reduced (<italic>P<</italic>0.05) after LCA (10, 20, and 40 μmol·L<sup>-1</sup>) treatment in a dose-dependent manner, leading to mitochondrial dysfunction. LCA (10, 20, and 40 μmol·L<sup>-1</sup>) induced ER stress to up-regulate the expression of CHOP, ATF4, p-PERK, and p-eIF2<italic>α</italic> (<italic>P<</italic>0.05) in a dose-dependent manner. Conclusion:LCA can induce MDA-MB-231 cell apoptosis by increasing intracellular ROS level and reducing MMP to trigger mitochondrial dysfunction and ER stress.

5.
Journal of Forensic Medicine ; (6): 615-618, 2017.
Article in Chinese | WPRIM | ID: wpr-692372

ABSTRACT

Objective To explore the application value of InnoTyper? 21 kit in forensic practice. Methods Samples of hair shafts and saliva were collected from 8 unrelated individuals. Template DNA was ex-tracted by AutoMate ExpressTM forensic DNA automatic extraction system. DNA was amplified by Inno-Typer? 21 kit and AmpFeSTRTM IdentifilerTM Plus kit, respectively, and then the results were compared. Results After the amplification by InnoTyper ? 21 kit, complete specific genotyping could be detected from the saliva samples, and the peak value of genotyping profiles of hair shafts without sheath cells was 57-1219 RFU. Allelic gene deletion could be found sometimes. When amplified by AmpFeSTRTM IdentifilerTM Plus kit, complete specific genotyping could be detected from the saliva samples , and the specific fragment was not detected in hair shafts without sheath cells. Conclusion The InnoTyper? 21 kit has certain application value in the cases of hair shafts without sheath cells.

6.
Biomedical and Environmental Sciences ; (12): 678-682, 2016.
Article in English | WPRIM | ID: wpr-296553

ABSTRACT

This study proposes a new framework as job burden-capital model for comprehensive assessment on occupational stress. 1618 valid samples were recruited from electronic manufacturing service industry in Hunan province by self-rated questionnaires after written consent. Structural equation model analysis was employed to verify the model by the data collected. The final fitting model has satisfactory fitting goodness (CMIN/DF=5.78, AGFI=0.937, NNFI=0.960, IFI=0.968, RMSEA=0.054). Both of the measurement model and structural model have acceptable path loadings. Job burden and capital could either directly affect occupational outcomes or indirectly influence them through personality. Multi-group structural equation model analyses indicate general applicability of the model to the basic features of such population. The job burden-capital model opens up new horizons for more in-depth study on occupational stress.


Subject(s)
Adult , Female , Humans , Male , Exploratory Behavior , Models, Theoretical , Occupations , Economics , Stress, Psychological , Economics , Psychology , Surveys and Questionnaires
7.
Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 48-51, 2013.
Article in Chinese | WPRIM | ID: wpr-242714

ABSTRACT

<p><b>OBJECTIVE</b>To explore the effects of mechanical irritation to the skin, eyes, and upper respiratory tract in workers exposed to rock wool.</p><p><b>METHODS</b>The gravimetric concentration of total dust and number concentration of fibre in the air of the rock wool workplace were determined . 109 rock wool manufacturing and processing workers were taken as the exposed group, 104 workers from an air separation plant, a papermaking plant and cover making plant were served as control group. All subjects accepted the questionnaire interview and clinical examination of the skin, eyes, nose and the pharynx.</p><p><b>RESULTS</b>10.1% of the exposed group had the irritant contact dermatitis, which showed linear trend with the current gravimetric concentration of total dust (P < 0.05) but no association with the exposure age (P > 0.05), and the detection rate of conjunctivitis of the exposed group (12.8%) was significantly higher than the control group (2.8%) (P < 0.05). The percentages of shin and eye itching symptoms of the exposed group (54.1% and 42.9%) were significantly higher than the control group (11.5% and 26.5%) (P < 0.05), but the differences among/between the exposed subgroups with different exposure level and different exposure age were not statistically significant (P > 0.05). The differences of the detection rate of chronic rhinitis and chronic pharyngitis and the percentages of the symptoms of nose and pharynx between the exposed group and control group were not statistically significant (P > 0.05), except the rhinorrhea symptom.</p><p><b>CONCLUSIONS</b>Occupational exposure to rock wool had some degree of the mechanical irritation effects on the skin and eyes. The current exposure level of total dust should be emphatically controlled.</p>


Subject(s)
Adult , Humans , Male , Middle Aged , Asbestos , Case-Control Studies , Conjunctivitis , Epidemiology , Dermatitis, Irritant , Epidemiology , Dust , Occupational Exposure , Physical Stimulation , Workplace
8.
Journal of Forensic Medicine ; (6): 209-211, 2013.
Article in Chinese | WPRIM | ID: wpr-983824

ABSTRACT

OBJECTIVE@#To establish a new method for RNA and DNA co-extraction from the same sample by TRIzol reagent.@*METHODS@#After the aqueous phase which contained total RNA was removed by traditional TRIzol method, the values of pH of the interphase phase and organic phase were adjusted. The DNA was precipitated with ethanol and purified with DNA IQ system. The purified DNA was measured in quality and quantity. As the template, it was amplified and typed by PCR-STR. The data was compared with that extracted by traditional TRIzol method.@*RESULTS@#The DNA extracted by this modified method showed a better result of quality and quantity than that by traditional TRIzol method and a good STR typing.@*CONCLUSION@#The modified TRIzol method is advisable and reliable to simultaneously extract both DNA and RNA from the same sample. It could be used for individual identification and paternity testing to satisfy the need of forensic science.


Subject(s)
Humans , Blood Chemical Analysis/methods , DNA/isolation & purification , DNA Fingerprinting , Forensic Medicine , Guanidines/chemistry , Hydrogen-Ion Concentration , Phenols/chemistry , Polymerase Chain Reaction , RNA/isolation & purification , Reagent Kits, Diagnostic
9.
Chinese Journal of Oncology ; (12): 937-939, 2012.
Article in Chinese | WPRIM | ID: wpr-284254

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of isolated limb hyperthermic perfusion chemotherapy for melanoma of the extremities.</p><p><b>METHODS</b>Limb isolated hyperthermic perfusion chemotherapy was performed in 41 patients with malignant melanoma of the extremities, and then the primary lesions in 24 patients were removed at 14 - 21 days after chemotherapy. Tumor necrosis was examined by pathology.</p><p><b>RESULTS</b>Among the 41 patients, 40 cases were followed up for 6-113 months, and one was lost. There was no local recurrence in those patients. 29 cases were followed up for more than 3 years, and 26 of them were surviving. Forteen cases were followed up for more than five years, among them 9 cases were surviving. The 3-year and 5-year survival rates of the whole group were 95.0% and 70.0%, respectively. The average reduction of the tumor volume was 55.6% after perfusion. The pathological examination showed that tumor necrosis was 90% - 100% (complete response) in 21 cases (87.5%) and 60% - 89% (partial response) in 3 cases (12.5%).</p><p><b>CONCLUSIONS</b>The isolated limb hyperthermic perfusion chemotherapy is an effective treatment of limb malignant melanoma. It can significantly reduce the local recurrence rate, and improve the 5-year survival rate, prognosis and the quality of life of the patients.</p>


Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Chemotherapy, Cancer, Regional Perfusion , Methods , Cisplatin , Extremities , Follow-Up Studies , Hyperthermia, Induced , Melanoma , Drug Therapy , Quality of Life , Remission Induction , Soft Tissue Neoplasms , Drug Therapy , Survival Rate
10.
Chinese Journal of Hepatology ; (12): 348-352, 2012.
Article in Chinese | WPRIM | ID: wpr-262004

ABSTRACT

<p><b>UNLABELLED</b>To analyse the live pathology characteristics in mild ALT-elevated (1 x ULN less than ALT less than 2 x ULN ) HBeAg-positive and HBeAg-negative chronic hepatitis B (CHB) patients, and to explore the influence of the age and HBV DNA level to liver pathology in different HBeAg status patients.</p><p><b>METHODS</b>All the patients who met the inclusion criteria form "eleventh five-year plan" National Science and Technology Major Project, the treatment program of integrative traditional and western medicine for CHB were enrolled in this study between October 2009 and March 2011 .B type ultrasound-guided liver biopsy was carried out in all patients and hepatitis B surface antigen (HBsAg) , HBeAg titer as well as HBV DNA level were detected at the same time. Hepatic tissue inflammation and fibrosis degree of patients according to HBeAg-positive and negative, age ( more than or equal to 40 years and less than 40 years), HBV DNA level (more than or equal to 10


Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Alanine Transaminase , Blood , Hepatitis B e Antigens , Blood , Hepatitis B, Chronic , Blood , Pathology , Liver , Pathology
11.
Chinese Journal of Integrated Traditional and Western Medicine ; (12): 499-503, 2012.
Article in Chinese | WPRIM | ID: wpr-252481

ABSTRACT

<p><b>OBJECTIVE</b>To study the effects of Lingmao Recipe (LR) on starvation-induced autophagy in HepG 2.2.15 cell.</p><p><b>METHODS</b>Fifteen SD rats were selected to prepare LR drug serum. The Earle's balanced salt solution (EBSS) group, the EBSS + vehicle serum group, the EBSS + 5 times LR serum group, the EBSS + 10 times LR serum group, and the DMEM group were set up, 3 samples in each group. The HepG2.2.15 cells were cultured for 1, 2, 4, and 6 h respectively. The pEGFP-N1-LC3B eukaryotic expression vector was constructed and transfected with HepG2. 2.15 cell. The GFP-LC3B morphological changes were observed under fluorescent microscope. The ratio of the dotty GFP-LC3B number and the GFP-LC3B transfected HepG2.2.15 number was calculated. The intracytoplasmic autophagosome changes were observed using electronic transmission electron. The microtubule-associated protein 1 light chain 3 beta II/I was detected in HepG2.2.15 of each group using Western blot.</p><p><b>RESULTS</b>Two h after culture, when compared with the EBSS + vehicle serum group, GFP-LC3B changing from diffused distribution to dotted distribution was obviously inhibited in the EBSS +5 times LR serum group and the EBSS +10 times LR serum group (P<0.01). The electronic transmission electron showed that the formation of autophagosome was inhibited in the EBSS +5 times LR serum group and the EBSS +10 times LR serum group. Results of Western blot showed that microtubule-associated protein 1 light chain 3 beta II/I obviously decreased more in the EBSS +10 times LR serum group than in the EBSS +vehicle serum group (P<0.01).</p><p><b>CONCLUSION</b>LR could inhibit starvation-induced autophagy in HepG 2.2.15.</p>


Subject(s)
Animals , Humans , Male , Rats , Autophagy , Culture Media , Drugs, Chinese Herbal , Pharmacology , Hep G2 Cells , Rats, Sprague-Dawley , Serum , Transfection
12.
Journal of Forensic Medicine ; (6): 359-361, 2012.
Article in Chinese | WPRIM | ID: wpr-983762

ABSTRACT

OBJECTIVE@#To explore the tissue-specific gene expressions of the peripheral blood and the menstrual blood, and to search some specific factors to establish an effective method for identifying the peripheral blood and the menstrual blood.@*METHODS@#The specific products of the peripheral blood and the menstrual blood were detected by RT-PCR and separated by electrophoretic technology.@*RESULTS@#Beta-spectrin (SPTB) as one specific marker of peripheral blood and 18S rRNA as a kind of the housekeeping gene were expressed in both the peripheral blood and the menstrual blood. However, matrix metalloproteinase 7 (MMP7) as one specific marker of menstrual blood and human beta defensin 1 (HBD1) as one specific marker of vaginal discharge were only found in the menstrual blood.@*CONCLUSION@#There are differences of specific gene expressions between the peripheral blood and the menstrual blood. They could be accurately distinguished from each other by using the combination of fluorescence technology and RT-PCR to detect the specific identification of mRNA.


Subject(s)
Female , Humans , Biomarkers , Blood/metabolism , Gene Expression , Gene Expression Profiling , Matrix Metalloproteinase 7/genetics , Menstruation/genetics , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain Reaction , beta-Defensins
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