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1.
China Tropical Medicine ; (12): 863-2023.
Article in Chinese | WPRIM | ID: wpr-1005155

ABSTRACT

@#Abstract: Objective To elucidate the potential mechanism of Jindanjiangan Capsule in the treatment of liver fibrosis by network pharmacology and molecular docking. Methods Active ingredients and targets of Jindanjiangan Capsules were searched by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and HERB databases, and the disease targets were screened by DisGeNET and Therapeutic Target Database (TTD) databases. The targets of the active ingredients of Jindanjiangan Capsule were matched with the disease targets, and the common targets were imported into the String database platform to construct a protein-protein interaction network (PPI) network. CytoNCA tool of Cytoscape 3.9.1 software was used for topological analysis to screen key targets. Traditional Chinese Medicine-Key Active IngredientsKey Target Network was constructed by Cytoscape 3.9.1 Software. KEGG enrichment analysis of key targets was performed through the DAVID platform. The molecular docking of active ingredients and targets was performed to verify the above results using LeDock software. Results By screening, 180 potential active ingredients and 1 340 targets of Jindanjiangan Capsule and 1 060 targets of liver fibrosis, and 273 common targets were obtained. 29 key targets related to liver fibrosis were screened out by PPI network interaction, and verified by KEGG analysis and molecular docking. Jindanjiangan capsule acts on key targets such as EGFR, MMP9, PTGS2, ESR1, PIK3CA, F2, PPARG, and PTPN11 through active components such as isovitexin, quercetin 7-O- β -D-glucoside, (3S, 6S) -3- (benzyl) -6- (4-hydroxybenzyl) piperazine-2, 5-quinone, 6-Osyringoyl-8-O-acetylshanzhiside methyl ester, tanshinone II, nortanshinone, capillaris chromone, and etanone. The specific mechanism may be related to HIF-1 signaling pathway, C-type lectin receptor signaling pathway, Toll-like receptor signaling pathway, tumor necrosis factor signaling pathway, relaxin signaling pathway, FoxO signaling pathway and so on. Conclusion Jindanjiangan capsule can effectively treat hepatic fibrosis through multi-component, multi-target and multi-pathway.

2.
Rev. bras. med. esporte ; 29: e2022_0592, 2023. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1423462

ABSTRACT

ABSTRACT Introduction: In track and field events, the force generated by running, jumping, and throwing starts from the lower limbs and is transmitted from bottom to top, forming a complete dynamic chain. Objective: Study the effect of different combined strength training methods on the explosive strength of the lower limbs of track and field athletes. Methods: Twenty members of the track and field team were selected as research subjects and divided into two groups, A and B. Group A performed the maximum resistance training method, and group B performed the submaximal resistance training method and underwent 12 weeks of different combined strength training. Results: After 12 weeks of training, there was a significant difference in the results of the two groups of students in the approach run (male P=0.034, female P=0.021), and the results of experimental group B improved significantly. Conclusion: The maximum resistance training method can most effectively improve the explosive performance of the lower limbs of track and field practitioners. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.


RESUMO Introdução: Em eventos de atletismo, a força gerada pela corrida, salto e arremesso parte dos membros inferiores e é transmitida de baixo para cima, finalmente formando uma cadeia dinâmica completa. Objetivo: Estudar o efeito de diferentes métodos combinados de treinamento de força sobre a força explosiva dos membros inferiores dos esportistas de atletismo. Métodos: Foram selecionados 20 membros da equipe de atletismo como objetos de pesquisa e divididos em dois grupos, A e B. O grupo A realizou o método de treinamento de resistência máxima, e o grupo B realizou o método de treinamento de resistência submáxima, sendo submetidos a 12 semanas de diferentes treinamentos de força combinados. Resultados: Após 12 semanas de treinamento, houve uma diferença significativa nos resultados dos dois grupos de estudantes na corrida de aproximação (masculino P=0,034, feminino P=0,021), e os resultados do grupo experimental B melhoraram significativamente. Conclusão: O método de treinamento de resistência máxima pode melhorar de forma mais eficaz o desempenho explosivo dos membros inferiores dos praticantes de atletismo. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.


RESUMEN Introducción: En las pruebas de atletismo, la fuerza generada por la carrera, el salto y el lanzamiento parte de las extremidades inferiores y se transmite de abajo a arriba, formando finalmente una cadena dinámica completa. Objetivo: Estudiar el efecto de diferentes métodos combinados de entrenamiento de la fuerza sobre la fuerza explosiva de los miembros inferiores de los atletas de atletismo. Métodos: Se seleccionaron veinte miembros del equipo de atletismo como sujetos de investigación y se dividieron en dos grupos, A y B. El grupo A realizó el método de entrenamiento de resistencia máxima, y el grupo B realizó el método de entrenamiento de resistencia submáxima, y se sometió a 12 semanas de diferentes entrenamientos de fuerza combinados. Resultados: Después de 12 semanas de entrenamiento, hubo una diferencia significativa en los resultados de los dos grupos de estudiantes en la carrera de aproximación (hombre P=0,034, mujer P=0,021), y los resultados del grupo experimental B mejoraron significativamente. Conclusión: El método de entrenamiento de resistencia máxima puede mejorar de forma más eficaz el rendimiento explosivo de los miembros inferiores de los practicantes de atletismo. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

3.
Chinese Journal of School Health ; (12): 399-402, 2019.
Article in Chinese | WPRIM | ID: wpr-819281

ABSTRACT

Objective@#The purpose of this study was to determine the effect of climbing exercise intervention on gross motor function in children aged 8 to 9 years, and to provide a theoretical basis and scientific reference for the application of climbing exercise in the improvement of children health.@*Methods@#Random sampling method was used to select 53 children aged 8-9 from a public school in Haidian District in Beijing, and children were randomly divided into control group (n=30) and exercise group (n=23), children in the exercise group received climbing exercises for 8 weeks . KTK test was used to assess gross motor development.@*Results@#After intervention, the gross motor level of male and female children in Exercise group were significantly higher than before (in male group, before intervention, KTK score:217.5±32.3,after intervention, KTK score:245.1±24.2,P<0.01;in female group, before intervention, KTK score: 219.6±30.3, after intervention, KTK score: 246.9±23.2,P<0.01); while the gross motor level in control group had no significant change compared to the intervention state before(in male group, before intervention, KTK score:219.9±26.8,after intervention, KTK score:224.8±30.2,P>0.05;in female group, before intervention, KTK score: 216.6±18.1,after intervention, KTK score:214.8±28.6,P>0.05). There were no differences of gross motor level between sham and exercise group at baseline (P>0.05). After intervention, except the MS scores (P>0.05), other test program scores were higher than those of control group (in exercise group, WB: 52.1±6.8; HH: 61.7±8.7; JS: 73.7±7.6; in control group, WB: 43.1±9.9; HH: 54.5±15.2; JS: 64.9±6.8) (P<0.01).@*Conclusion@#Our research shows that climbing exercise intervention helps promoting the children’s physical quality, and establishes substantial foundation for the formation and promotion of sports cognition and complex motor skills.

4.
Neuroscience Bulletin ; (6): 67-78, 2019.
Article in English | WPRIM | ID: wpr-775464

ABSTRACT

The rostral ventrolateral medulla (RVLM) is a key region in cardiovascular regulation. It has been demonstrated that cholinergic synaptic transmission in the RVLM is enhanced in hypertensive rats. Angiotensin-converting enzyme 2 (ACE2) in the brain plays beneficial roles in cardiovascular function in hypertension. The purpose of this study was to determine the effect of ACE2 overexpression in the RVLM on cholinergic synaptic transmission in spontaneously hypertensive rats (SHRs). Four weeks after injecting lentiviral particles containing enhanced green fluorescent protein and ACE2 bilaterally into the RVLM, the blood pressure and heart rate were notably decreased. ACE2 overexpression significantly reduced the concentration of acetylcholine in microdialysis fluid from the RVLM and blunted the decrease in blood pressure evoked by bilateral injection of atropine into the RVLM in SHRs. In conclusion, we suggest that ACE2 overexpression in the RVLM attenuates the enhanced cholinergic synaptic transmission in SHRs.


Subject(s)
Animals , Male , Rats , Acetylcholine , Metabolism , Blood Pressure , Physiology , Cardiovascular System , Metabolism , Cholinergic Neurons , Metabolism , Hypertension , Metabolism , Peptidyl-Dipeptidase A , Metabolism , Rats, Inbred SHR , Metabolism
5.
Journal of Prevention and Treatment for Stomatological Diseases ; (12): 227-230, 2018.
Article in Chinese | WPRIM | ID: wpr-778722

ABSTRACT

Objective @#To assess hypoxia-inducible factor 1α (HIF-1α) expression levels in the periodontal tissues of the pressure side during orthodontic tooth movement in rats.@*Methods@#A total of 50 male 6-week-old Sprague Dawley (SD) rats were randomly divided into 10 groups of 5 rats each. The upper left first molar was the experimental tooth and was pulled mesially with an orthodontic force of 10-15 g for 0, 1, 3, 6, or 12 h, or 1, 3, 7, 14, or 21 d. Routine five-micrometer paraffin-embedded tissue sections were processed for HE staining and immunohistochemical staining for HIF-1α. The Image-Pro Plus system was used to quantitatively analyze the stained slices. The expression of HIF-1α in the periodontal tissue of the pressure side changed during the process of orthodontic tooth movement.@*Results@#The expression of HIF-1α increased immediately after loading for 1 h, reached a small peak at 3 h, and then decreased. After 12 h, the expression increased again, reached a peak after 1 d, and then gradually decreased to near the pre-loading level(P < 0.05). @*Conclusion@#There were differences in expression of HIF-1α in different groups in the periodontal tissues of the pressure side during orthodontic tooth movement in rats.

6.
China Journal of Chinese Materia Medica ; (24): 1934-1939, 2018.
Article in Chinese | WPRIM | ID: wpr-690692

ABSTRACT

To systematically evaluate the efficacy and safety of external therapies of traditional Chinese medicine(TCM) combined with sodium hyaluronate(SH) injected in articular cavity therapy on knee osteoarthritis(KOA). The following databases such as CNKI, WanFang, VIP, CBM, PubMed and Medline were researched to collect the randomized controlled trails on external therapies of TCM combined with sodium hyaluronate injected in articular cavity therapy on KOA. The selection of studies, assessment of methodological quality and data extraction were performed independently by two researchers. The methodological quality was assessed by using the Cochrane system evaluation methodology and Meta-analysis were performed by using Cochrane Collaboration's the RevMan 5.3 software. Forteen studies involving 1 449 patients were included. All of the trails were not adequate enough in methodological quality. Meta-analysis indicated that compared with control group, external therapies of TCM combined with sodium hyaluronate injected in articular cavity could raise effectiv rate(<0.000 01) and cure-rate(<0.000 01), improve Lysholm score(=0.003) and reduce VAS score(<0.000 1). But two groups have no difference in Womac score (=0.13).Compared with the treatment with sodium hyaluronate injected in articular cavity, external therapies of TCM combined with sodium hyaluronate injected in articular cavity, a promising treatment options, can be complementary advantages, improve the clinical curativ effect. But it still needs low risk and high quality clinical trials to verify.

7.
Chinese Journal of Cancer Biotherapy ; (6): 469-474, 2018.
Article in Chinese | WPRIM | ID: wpr-821250

ABSTRACT

@#[Abstract] Objective: To explore the mechanism of glucose transport protein-1(Glut-1) promoting the migration of osteosarcoma MG63 cells through Wnt/β-catenin pathway. Methods: RNA interference recombinant adenovirus targeting Glut-1 gene (Ad-Glut-siRNA) and control recombinant adenovirus (Ad-GFP) were constructed and transfected into MG63 cells to silence Glut-1 gene expression. The cell migration ability of Blank group, Ad-AFP group, Ad-Glut-siRNA group and AZD2858 (inhibitor of GSK-3) group were detected by Transwell chamber migration assay. Immunofluorescence assay was used to detect the expression of E-cadherin and vimentin in each group and the nuclear translocation of β-catenin. The expression of MMP-2 and MMP-9 in each group and FZD7, β-catenin, Dsh protein in Blank group, Ad-AFP group, Ad-Glut-siRNA group were detected by Western blotting assay. Results: The migration ability of MG63 cells was significantly decreased (P<0.05) after Glut-1 gene silencing, which was restored afterAZD2858 treatment (P <0.05). Compared with Blank group and Ad-GFP group, the E-cadherin level in MG63 cells in Ad-Glut-siRNA group was significantly increased (P<0.05), while the expressions of vimentin, MMP-2, MMP-9, FZD7, β-catenin and Dsh protein were significantly reduced (all P<0.05). Compared with Ad-Glut-siRNA group, E-cadherin expression of AZD2858 group was significantly reduced, while the expressions of vimentin, MMP-2, MMP-9 were significantly up-regulated (P<0.05). Conclusion: The high expression of Glut-1 gene is closely related to the invasion and metastasis of MG63 cells. The possible mechanism is that the high expression of Glut-1 leads to the activation of Wnt/β-catenin pathway, which leads to the decrease of EMT-related protein E-cadherin, and the increase of vimentin and MMP-2, MMP-9, and further promotes the migration of MG63 cells.

8.
Int. braz. j. urol ; 43(5): 849-856, Sept.-Oct. 2017. tab, graf
Article in English | LILACS | ID: biblio-892898

ABSTRACT

ABSTRACT Objectives: To evaluate the prognosis of non-metastatic T3a renal cell carcinoma (RCC) with partial nephrectomy (PN). Patients and Methods: We retrospectively evaluated 125 patients with non-metastatic T3a RCC. Patients undergoing PN and radical nephrectomy (RN) were strictly matched by clinic-pathologic characteristics. Log-rank test and Cox regression model were used for univariate and multivariate analysis. Results: 18 pair patients were matched and the median follow-up was 35.5 (10-86) months. PN patients had a higher postoperative eGFR than RN patients (P=0.034). Cancer-specific survival (CSS) and recurrence-free survival (RFS) did not differ between two groups (P=0.305 and P=0.524). On multivariate analysis, CSS decreased with positive surgical margin and anemia (both P <0.01) and RFS decreased with Furhman grade, positive surgical margin, and anemia (all P<0.01). Conclusions: For patients with non-metastatic pT3a RCC, PN may be a possible option for similar oncology outcomes and better renal function.


Subject(s)
Humans , Male , Female , Carcinoma, Renal Cell/surgery , Kidney Neoplasms/surgery , Nephrectomy/methods , Prognosis , Carcinoma, Renal Cell/pathology , Retrospective Studies , Treatment Outcome , Disease-Free Survival , Kidney Neoplasms/pathology , Middle Aged
9.
International Eye Science ; (12): 2351-2353, 2017.
Article in Chinese | WPRIM | ID: wpr-669372

ABSTRACT

·AIM:To investigate the therapeutic effect of endoscopic dacryocystorhinostomy in the treatment of acute dacryocystitis.·METHODS: Totally 55 patients with acute dacryocystitis was divided into two groups according to the clinical manifestations:the group of redness and swelling and the group of abscess rupture. The bleeding and pain after operation were observed. Operation effect and skin scar of lacrimal sac area was observed and compared between two groups.·RESULTS: The patients were followed up from 3mo to 2a, and effective rates of the two groups of patients were compared, which showed no statistically significant difference ( x2 = 0. 307, P> 0. 05 ). Surgery before the abscess ruptured had the minimal impact on the appearance. The appearance impact of the group of abscess rupture was higher than that of the group of redness, and there are statistically significant difference (x2=12. 44, P<0. 05).·CONCLUSION:Endonasal endoscopic dacryocystorhinostomy is an effective method on the treatment of acute dacryocystitis. Early surgery has the advantages of quick recovery, scarlessness and high efficiency. Therefore, it worthy of clinical promotion.

10.
Bol. latinoam. Caribe plantas med. aromát ; 14(6): 462-469, Nov. 2015. tab
Article in English | LILACS | ID: biblio-907508

ABSTRACT

In the present study, the repellent activities of the leaf and/or stem crude extracts of Glycosmis lucida Wall. ex Huang, G. craibii var. glabra, G. craibii Tanaka, G. oligantha Huang, G. pentaphylla (Retz) Correa. and G. esquirolii (Levl.) Tanaka were analyzed by using assays on petri dishes against Tribolium castaneum and Liposcelis bostrychophila. The leaf and stem extracts of G. lucida, G. craibii var. glabra, G. craibii Tanaka, G. oligantha and G. esquirolii possessed significant repellent activities against T. castaneum, the same level repellent with the positive control, DEET. However, the extracts of G. pentaphylla, no repellency but some insect attractant was observed. Moreover, they also showed repellent activities against L. bostrychophila. These results indicate that extracts from G. lucida and G. oligantha leaf could be a source of novel repellent against insects.


En el presente estudio, las actividades repelentes de la hoja y/o tronco de los extractos crudos de Glycosmis lucida Wall. ex Huang, G. craibii var. glabra, G. craibii Tanaka, G. oligantha Huang, G. pentaphylla (Retz) Correa y G. esquirolii (Levl.) Tanaka se analizaron mediante el uso de ensayos en placas de Petri contra Tribolium castaneum y Liposcelis bostrychophila. Los extractos de las hojas y tallo de G. lucida, G. craibii var. glabra, G. craibii Tanaka, G. oligantha y G. esquirolii poseían actividades repelentes significativas contra T. castaneum, el mismo nivel repelente del control positivo, el DEET. Sin embargo, los extractos de G. pentaphylla, no se observó la repelencia pero sí actividad atrayente de insectos. Por otra parte, también se mostraron las actividades repelentes contra L. bostrychophila. Estos resultados indican que los extractos de hojas de G. lucida y G. oligantha podrían ser una fuente de repelente contra los insectos.


Subject(s)
Insecta , Insect Repellents/pharmacology , Plant Extracts/pharmacology , Plant Leaves/chemistry , Rutaceae/chemistry , Tribolium
11.
Indian J Pathol Microbiol ; 2013 Jul-Sept 56 (3): 300-302
Article in English | IMSEAR | ID: sea-155892

ABSTRACT

Extra-uterine placental site trophoblastic tumor (PSTT) is extremely rare. To our knowledge, PSTT that occurs in the pelvic wall has not yet been reported. A 29-year-old woman presented with amenorrhea and irregular vaginal bleeding of 1 month. A solid tumor mass was detected by ultrasonography in the right pelvic wall. The tumor was comprised of large, polygonal tumor cells, with brisk mitosis and obvious vascular invasion. Immunohistochemical staining demonstrated that tumor cells were positive for human placental lactogen, CD146, cytokeratin, placental alkaline phosphatase, human chorionic gonadotropin were positive, the Ki-67 proliferative index was about 80%. The pathological diagnosis was PSTT. After the operation, the patient was treated with six cycles of etoposide, methotrexate, actinomycin, cyclophosphamide, and vincristine. The patient was followed for 18 months without recurrence. The report shows that extra-uterine PSTT is extremely rare and may have a good prognosis; surgical resection and adjuvant chemotherapy are good options. However, further experience to diagnose and cure this rare tumor is warranted.

12.
Chinese Journal of Hematology ; (12): 594-598, 2010.
Article in Chinese | WPRIM | ID: wpr-353610

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the correlation between the platelet GP specific antibodies/HLA antibodies and platelet transfusion refractoriness (PTR).</p><p><b>METHODS</b>Sixty-five patients with PTR were selected in this study and were genotyped for HLA-A and HLA-B as well as HPA systems by standard PCR-SSP assays. The platelet GP specific antibodies and HLA antibodies in serum and platelet elution were tested with a solid phase ELISA.</p><p><b>RESULTS</b>The HLA-A/B antigens and the frequencies of HPA-1, 2, 4, 5, 6, 9, 15 antigens in PTR patients had no difference from those in healthy donors. The freguencies of HPA-3a and 3b were 0.65 and 0.35, respectively. There was statistical difference between the 65 PTR patients and the healthy donors in HPA-3 freguencies (P < 0.05). Twenty-four patients (36.9 %) only expressed HLA antibodies, and 14 (21.5%) expressed HLA and platelet GP specific antibodies. The highest expression of anti-HLA-A/B specific antibodies was -A*9(46.2 %)/-B*40(33.6%), respectively. In serum, GPIIb/IIIa was expressed (26.2%), followed by GPIa/IIa (21.5 %). In platelet elution, GPIIb/IIIa was expressed of 41.5% and GPIb/IX 41.5%. Pedigree study was carried out for 2 patients. The results showed that the platelet GP specific antibody/HLA antibody developed in PTR patients was highly related to the mismatch with the platelet antigen/HLA antigen in their parents.</p><p><b>CONCLUSION</b>The expressions of the HLA and platelet GP specific antibodies are the most important reason in PTR, it's meaningful to explore the correlation between PTR and HLA and HLA-A/B antigen in guiding platelet transfusion.</p>


Subject(s)
Humans , Antigens, Human Platelet , Allergy and Immunology , Blood Platelets , Isoantibodies , Allergy and Immunology , Platelet Transfusion , Thrombocytopenia
13.
Journal of Experimental Hematology ; (6): 1032-1035, 2009.
Article in Chinese | WPRIM | ID: wpr-343356

ABSTRACT

In order to investigate the expression of the anti-platelet glycoprotein specific antibodies and anti-HLA antibodies in idiopathic thrombocytopenic purpura (ITP), 45 patients with ITP were selected in this study. An easy PCR-SSP assay was used to detect single-nucleotide polymorphisms or deletion in HPA and HLA systems. The anti-platelet glycoprotein specific antibodies and anti-HLA antibodies in plasma or platelet eluate were tested with a solid phase ELISA. The results indicated that the anti-platelet glycoprotein specific antibodies were detected in plasma or platelet eluate of 45 patients, among which anti-GPIIb/IIIa/and anti-GpIb/IX were most common. Both the anti-platelet glycoprotein specific antibodies and anti-HLA antibodies were found in plasma of 11 patients. Pedigree investigation in 2 patients (case 37 and case 40) was carried out, the results showed that anti-platelet glycoprotein specific antibodies and anti-HLA antibodies detected in 2 patients closely related to incompatibility with platelet antigens and HLA antigens in parents. In conclusion, the results suggested that detection of the anti-platelet glycoprotein specific antibodies and anti-HLA antibodies in plasma or platelet eluate in combination with investigation of clinical manifestation of patients is important for diagnosis of idiopathic thrombocytopenic purpura.


Subject(s)
Adolescent , Adult , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Middle Aged , Young Adult , Antibodies, Anti-Idiotypic , Blood , Antigens, Human Platelet , Allergy and Immunology , HLA Antigens , Allergy and Immunology , Platelet Glycoprotein GPIIb-IIIa Complex , Allergy and Immunology , Platelet Glycoprotein GPIb-IX Complex , Allergy and Immunology , Platelet Membrane Glycoproteins , Allergy and Immunology , Purpura, Thrombocytopenic, Idiopathic , Blood , Allergy and Immunology
14.
Journal of Experimental Hematology ; (6): 639-644, 2008.
Article in Chinese | WPRIM | ID: wpr-267920

ABSTRACT

This study was aimed to investigate the characteristics of human bone marrow mesenchymal stem cells during ex-vivo expansion, MSCs were isolated from human bone marrow. At each passage, the characteristics of proliferation kinetics, osteogenic and adipogenic differentiation potential were analyzed, and cell morphology, surface markers were investigated as well. The karyotype analysis was done in different passage cells. The infection HIV, HCV, HBV and TP were detected by ELISA. Mycoplasma contamination in vitro was detected by PCR method. HLA-SBT was used to reanalyze the results of HLA antigens and alleles. STR genetic loci were detected by PCR in the MSC1, MSC2, MSC3 and MSC4. The results indicated that the proliferative ability and osteogenic potential decreased with the increase of passage number during culture expansion. The multiple differentiation potential of MSCs was maintained during their life span. Karyotype analysis showed that MSCs from 4 groups before passage 8 were normal. The expression of CD29, CD44, CD105, CD166 and CD73 were positive. The expression of CD14, CD34, CD45, CD80, CD86 were all negative. SBT was used to identify HLA-A, B, Cw, DRB1, DRPB1, DQ alleles in the MSC1, MSC2, MSC3, MSC4. The genetype of STR in the MSC1, MSC2, MSC3, MSC4 was different. MSC 3 was examined by TP-ELISA to confirm the infectious disease of TP. MSC2 was contaminated by mycoplasma at passage 5. It is concluded that culture expansion causes MSCs to gradually lose their stem cell properties. During ex-vivo expansion of MSCs, the osteogenic differentiation potential is decreased. MSCs before passage 8 can be a valuable subject for basic research and clinical application.


Subject(s)
Adult , Female , Humans , Male , Adipogenesis , Bone Marrow Cells , Cell Biology , Cell Differentiation , Physiology , Cells, Cultured , Karyotyping , Mesenchymal Stem Cells , Cell Biology , Osteogenesis
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