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This study was aimed to investigate the effect of Rheum tanguticum on expression of serum endothelial nitric oxide synthase (eNOS), endothelin (ET) and 6-keto-prostaglandin F1α (6-Keto-PGF1α) in uric acid nephropathy rat model. After 3 weeks of treatment with different extractions of Rheum tanguticum on uric acid nephropathy rat model, levels of serum BUN, Cr and UA were measured by biochemical analyzer. ELISA method was used to measure the expression of serum eNOS, ET and 6-Keto-PGF1α. Meanwhile, pathological changes of renal tissues were observed by light microscope. The results showed that compared with the normal control group, the levels of serum BUN, Cr, UA and renal index were higher in the model group (P< 0.05). Compared with the model group, the concentration of eNOS in the model group was obviously higher in the rhubarb ethanol extraction group, rhubarb water extraction group, and allopurinol group (P< 0.05). Concentration of ET was obviously reduced in the rhubarb water extraction group and allopurinol group (P< 0.05). There was no statistical difference with the rhubarb ethanol extraction group. There was no significant difference in the concentration of 6-Keto-PGF1α among groups. Lots of inflammatory cells and uratic crystallization were observed by light microscope in renal tissues among rats in the model group. Damages of renal tissues in medication groups were alleviated than the model group. It was concluded that Rheum tanguticumcan alleviate the damage of uratic crystallization and inflammatory cell infiltration, increase the concentration of serum eNOS, and decrease the concentration of serum ET. Thus, it can reduce the damage of renal endothelial cells and protect renal functions. The effect of rhubarb ethanol extraction on reducing ET expression was less than the water extraction.
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10.3969/j.issn.1008-9691.2013.03.007
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@#Objective To assess the level of plasma thromboxane B2(TXB2),6-keto-prostaglandin F1α(6-keto-PGF1α) of patients with acute cerebral infarction,so as to know the changes of platelet function on acute cerebral vascular diseases.MethodsThe level of plasma TXB2,6-keto-PGF1α and TXB2/6-keto-PGF1α(T/6-K) of 205 patients with acute cerebral infarction were measured within 24 hours and on the 7th day and 14th day,and were compared with the normal controls(n=40).ResultsThe levels of plasma TXB2,6-keto-PGF1α of patients within 24 hours,on the 7th day and 14th day were significantly higher than those in the normal controls(P<0.01).The level of plasma TXB2 was the highest on the 7th day and decreased on 14th day.The level of plasma 6-keto-PGF1α was the highest on the 14th day.T/6-K was higher than those in the normal controls within 24 hours and on the 7th day;it was highest on the 7th day and decreased on 14th day to level of the normal controls(P>0.05).ConclusionThe determine on levels of plasma TXB2,6-keto-PGF1α has important significance for knowing the changes of platelet function on acute cerebral infarction.The balance between TXB2 and 6-keto-PGF1α is important in keeping the blood smoothly.
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@#Objective To assess the level of plasma thromboxane B2(TXB2),6-keto-prostaglandin F1α(6-keto-PGF1α) of patients with acute cerebral infarction,so as to know the changes of platelet function on acute cerebral vascular diseases.MethodsThe level of plasma TXB2,6-keto-PGF1α and TXB2/6-keto-PGF1α(T/6-K) of 205 patients with acute cerebral infarction were measured within 24 hours and on the 7th day and 14th day,and were compared with the normal controls(n=40).ResultsThe levels of plasma TXB2,6-keto-PGF1α of patients within 24 hours,on the 7th day and 14th day were significantly higher than those in the normal controls(P<0.01).The level of plasma TXB2 was the highest on the 7th day and decreased on 14th day.The level of plasma 6-keto-PGF1α was the highest on the 14th day.T/6-K was higher than those in the normal controls within 24 hours and on the 7th day;it was highest on the 7th day and decreased on 14th day to level of the normal controls(P>0.05).ConclusionThe determine on levels of plasma TXB2,6-keto-PGF1α has important significance for knowing the changes of platelet function on acute cerebral infarction.The balance between TXB2 and 6-keto-PGF1α is important in keeping the blood smoothly.
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Objective To investigate the protective effect of prostaglandin E1 (PGE1) on platelet during cardiopulmonary bypass (CPB).Methods A total of 24 patients undergoing cardiac valve replacement were randomly divided into the experimental group (n=12) and the control group (n=12).The levels of thromboxane B2 (TXB2) and 6-keto-prostaglandin F1?(6-K-PGF1?) in serum were measured at different intervals during and after CPB. The ultrastructure of platelets was also observed by transmission electron microscope.Results The levels of TXB2 and 6-K-PGF1? during CPB were significantly higher than those before CPB in the both groups(P
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This study was performed to investigate effect of dried powders and ethanol extracts of garlic flesh and peel on lipid metabolism and antithrombogenic capacity in 16-month-old rats. Forty Sprague-Dawley male rats weighing 618.1 +/- 6.5 g were blocked into five groups according to body weight and raised for 3 months with control and experimental diets containing 5% (w/w) of dried powders of garlic flesh or peel, or ethanol extracts from equal amount of each dried powder and control diet. Plasma and liver total lipids, triglyceride and total cholesterol, and plasma HDL-cholesterol, thromboxane B2 (TXB2), 6-keto- prostaglandin F1alpha (6-keto-PGF1alpha) concentrations were measured. Total, insoluble and soluble dietary fibers contents were highest in peel powder followed by fresh powder, and those in ethanol extracts of flesh and peel, especially soluble, very low. Plasma and liver total lipids, triglyceride, and total cholesterol concentrations were lower in all the garlic experimental groups compared to Especially, flesh and peel powder lowered plasma total lipids, triglyceride and total cholesterol concentrations markedly, and flesh powder and flesh ethanol extract lowered liver total lipids, triglyceride and total cholesterol concentration remarkably. Plasma TXB2 concentrations in garlic experimental groups were lower than that of control group, and 6-keto-PGF1alpha concentrations. In garlic experimental groups were higher than that of control group. Flesh ethanol extract group showed the lowest TXB2 and the highest 6-keto-PGF1alpha concentrations among experimental groups, so TXB2/6-keto-PGF1alpha ratio in flesh ethanol extract group was significantly lower than that of control group. Moreover, clotting time was significantly increased in flesh ethanol extract group as compared to control group. In conclusion, intakes of dried powders and ethanol extracts of garlic flesh and peel were effective in lowering lipid levels of liver and plasma. And also flesh ethanol extract diet was most effective in antithrombogenic activity among garlic experimental groups as TXB2/6-keto- PGF1alpha ratio in flesh ethanol extract group was significantly lower and clotting time was significantly increased in this group as compared to control group.
Subject(s)
Animals , Humans , Infant , Male , Rats , Body Weight , Cholesterol , Diet , Dietary Fiber , Ethanol , Garlic , Lipid Metabolism , Liver , Plasma , Powders , Rats, Sprague-Dawley , Thromboxane B2 , TriglyceridesABSTRACT
Objective To investigate the clinical significance of changes in vascular endothelial cells during the development of glucocorticoid-induced avascular necrosis of femoral head(GANFH),and the preventive and therapeutic effects of "Cugusu Keli",a mixture of traditional Chinese medicines,on GANFH in rabbit.Methods Forty-eight New Zealand white rabbits were randomly assigned to four groups,12 in each,as control group,model group,therapy group and prevention group.The contents of plasma von Willebrand's factor(vWF),6-keto-prostaglandin F1?(6-keto-PGF1?)and the activity of plasminogen activator inhibitor 1(PAI-1)were determined at the 2nd,4th,6th,8th and 12th week after treatment.Meanwhile,rabbits were sacrificed at the 4th,8th and 12th week,and both femoral heads of all rabbits were obtained for histopathological examination.Results Compared with control group,the level of vWF and activity of PAI-1 in model group were elevated since the 4th and the 2nd week,while the level of 6-keto-PGF1? increased since the 4th week(P0.05).Compared with the model group,the level of vWF and activity of PAI-1 in therapy group was lowered,while the level of 6-keto-PGF1? increased(P
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ObjectiveTo investigate the correlations between Herpes simplex virus (HSV) infection and acute myocardial infarction(AMI).MethodsSpecific antibodies and DNA to HSV-1 were measured in 51 patients with AMI(AMI group),42 patients with old myocardial infarction (OMI group) and 31 normal controls (NC group) who had no angiographic evidence of coronary artery disease.Moreover,the changes of and relationships between fibrinogen(Fg),6-Keto-prostaglandin-F 1? (6-Keto-PGF 1? ),Thromboxane B 2 (TXB 2 ),P-selection(GMP 140 ) and HSV-1 infection were determined in the three groups.ResultsThe prevalence of HSV-1IgG antibody was associated with AMI(OR4.266).Adjustment for the major cardiovascular risk factors didn't attenuate this correlation(OR3.321).Those who were HSV-1 positive in the AMI group had significantly higher levels of Fg,TXB 2 and GMP 140 and a significantly lower level of 6-Keto-PGF 1? than those who were HSV-1 negative in the same group.Correlation analysis revealed that the average concentration of IgG antibody to HSV-1 was positively associated with the levels of Fg,TXB 2 and GMP 140 but negatively associated with the level of 6-Keto-PGF 1? in the HSV-1 positive patients of the AMI group.ConclusionThere is a significant correlation between HSV-1 infection and AMI.HSV-1 infection is associated with Fg,TXB 2 ,6-Keto-PGF 1? and GMP 140 .
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[Objective] To observe the therapeutic effect of pressing- moxibustion on Baihui (DU20) point combined with electroacupuncture (EA) on cervical spondylosis (CS) of vertebral artery type. [Methods] A single - blind random - controll trial was carried out in 34 cases of CS of vertebral artery type. The cases were randomized into two groups: the treatment group (Group A, n = 17) treated with pressing- moxibustion on Baihui point and EA on bilateral Fengchi (GB20) point and Jiaji (EX - B2) point of impaired vertebrae and the control group (Gropup B, n = 17) with EA alone. Plasma contents of thromboxane 82 (TXB2), 6-keto-prostaglandin Fl alpha (6-keto-PGF1?) and their ratio were measured before and after treatment. [Results] In the treatment group, 9 were cured, 6 markedly effective, 2 effective and 0 ineffective and 4,6,5 and 2 in the control group respectively; the markedly effective rate was 88.2% which was superior to that (58.8%) in the control group (P
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Objective To investigate the characteristics of renal hemodynamic and the esoteric prostacyclin(PGI2),thromboxane A2(TXA2)level in children with early Henoch-Schonlein purpura(HSP),and study the function of TXB_2/6-Keto-prostaglandin F(6-Keto-PGF_(1?))(T/K)numerus in early changes of kidney injury.Methods Children involved in the experiment were dicided into 3 groups.Thirty-one patients with HSP,divided into 2 groups according to routine urianlysis:children with HSP without renal damage group(n=16)and Henoch-Schonlein purpura nephritis(HSPN)group(n=15).Control group with 16 healthy children,their age and sex match with the other 2 groups.The urine of all children,including the children in control group,was sampled in 24 hours.The urinary production of the samples were kept in the freezer at-20 ℃.The radioimmunoassay was applied to determine the 6-Keto-PGF_(1?),TXB_2 quantitatively,and calculate the number of T/K.In the early morning the children accept the Doppler arteria renalis sonography with an empty stomach to determine the Vmax of the period of contraction of the arteria renalis the Vmin of diastolic phase and the resistent index(RI).SPSS 13.0 software was used to analyze the data.Results 1.The renal hemodynamic indicated a change of high velocity and resistance,the masculine rate(83.9%)was ob-viously higher than that in routine urinalysis(48.4%)(?2=5.79 P0.05).The RI in the former group(0.798?0.165)was much higher than that in the other one(0.637?0.116)(t=4.02 P
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Acute ligation of the bilateral common carotid artery and left vetebro-ar-tery resulted in an experimental incomplete cerebral ischemia in rabbits. It was consideredthat such experimental model could be used for studying the pathogenesis of brainischemia and the effect of experimtal therapy. 654-2 (anisodamine) was given at 30 and 60 min. after ischemia. The results indi-cated that: (1) The reducing rate of the cerebral blood flow in 654--2 group after ischemiawas much lower than that in control group (P