ABSTRACT
PURPOSE: CD5+ B (B1) cell is a subpopulation of B cells and CD5+ B cells constitute a large fraction of B cells in neonates. CD5 B cells are closely related with autoimmune diseases but the roles and functions in neonates are still unknown. The quantitative changes of CD5+ B cells in neonatal infections were examined to investigate the involvement of CDS+ B cells in neonatal immune reaction to general immunologic stimuli such as infections. Methods: Ten normal neonates and eight neonates with acute febrile diseases were studied. Venous blood was drawn and mononuclear cells were separated by Ficoll-Hypaque. Half was double-stained with FITC-conjugated anti-CD5 and PE-conjugated anti-CD19, and another half with FITC-conjugated anti-CD4 and PE-conjugated anti-CD8. Stained samples were analyzed using fluorescent-activated cell sortor. ...continue...
Subject(s)
Humans , Infant, Newborn , Autoimmune Diseases , B-LymphocytesABSTRACT
We investigated the changes of CD5+ B cells in the peripheral blood of 20 Kawasaki disease (KD) patients. The percentage of CD5+ B cells in the total lymphocytes and in the total B cells significantly decreased during the acute phase of KD(p< 0.01), compared to that in the age-matched normal control subjects. After intravenous immunoglobulin(IVIG) treatment, the percentage of CD5+ B cells increased, but was still lower than that in the normal controls(p< 0.01). During the convalescent phase of the disease, the percentage of CD5+ B cells was restored to the normal levels. The levels of CD5+ B cell percentage in the total B cells of the patients with acute febrile disease showed similar levels to age-matched normal controls. The decreased CD5+ B cells in the patients with KD provides an additional abnormal immunological finding during the acute phase of the disease.
Subject(s)
Child , Female , Humans , Infant , Male , Acute Disease , CD5 Antigens/analysis , B-Lymphocytes/immunology , Mucocutaneous Lymph Node Syndrome/immunology , Reference ValuesABSTRACT
The effect of intravenous immune globulin (IVIG) on the lymphocyte phenotypes in acute Kawasaki disease (KD) was studied in a random trial of IVIG-and-aspirin versus aspirin-alone. Before therapy, patients in each treatment group had an increased percentage of B cells, and a decreased percentage of T cells, CD4 T cells, CD8 T cells and CD5+ B cells. There was no significant difference in immunologic parameters between the two groups measured before therapy. Patients treated with IVIG-and-aspirin had by the fourth day developed a highly-significant increase in T cells, CD4 T cells and CD8 T cells and a decrease in B cells. Despite the decrease of B cells, there were significant increases in CD5+ B cells in both treatment groups. However, the degree of increase in the IVIG-and-aspirin treated group was significantly more noticeable than that in the aspirin-alone treated group. These findings indicate that treatment with IVIG restores the T- and B- cell abnormalities, especially CD5+ B-cell abnormalities found in patients with acute KD.
Subject(s)
Child, Preschool , Female , Humans , Infant , Male , Immunoglobulins, Intravenous/therapeutic use , Immunophenotyping , Lymphocyte Subsets/immunology , Mucocutaneous Lymph Node Syndrome/immunologyABSTRACT
The effect of intravenous immune globulin (IVIG) on the lymphocyte phenotypes in acute Kawasaki disease (KD) was studied in a random trial of IVIG-and-aspirin versus aspirin-alone. Before therapy, patients in each treatment group had an increased percentage of B cells, and a decreased percentage of T cells, CD4 T cells, CD8 T cells and CD5+ B cells. There was no significant difference in immunologic parameters between the two groups measured before therapy. Patients treated with IVIG-and-aspirin had by the fourth day developed a highly-significant increase in T cells, CD4 T cells and CD8 T cells and a decrease in B cells. Despite the decrease of B cells, there were significant increases in CD5+ B cells in both treatment groups. However, the degree of increase in the IVIG-and-aspirin treated group was significantly more noticeable than that in the aspirin-alone treated group. These findings indicate that treatment with IVIG restores the T- and B- cell abnormalities, especially CD5+ B-cell abnormalities found in patients with acute KD.