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In this study, a high-performance liquid chromatography method was established to simultaneously determine three flavonoids including hesperidin (HES), nobiletin (NOB) and tangeretin (TAN) in 10 batches of Citrus reticulata 'Chachi' planted and collected in Xinhui District, Jiangmen City, Guangdong Province. Moreover, we studied the metabolism and transformation of three flavonoids in liver and intestinal flora in vitro, and sequenced 16S rRNA of bacteria flora samples after incubation. The RP-HPLC system consisted of Alltima C18 column (250 mm × 4.6 mm, 5 μm) and a mobile phase of water (A) - methanol (B). The column temperature was 25 ℃ and the detection wavelength was both 283 nm and 330 nm while the flow rate was 1.0 mL·min-1. The results showed that the retention time of HES, NOB and TAN ranged from 12.313 min to 34.271 min. The content of HES, NOB and TAN in 10 batches of Citrus reticulata 'Chachi' was 26.81-39.80 mg·g-1, 4.06-7.90 mg·g-1 and 1.81-3.93 mg·g-1, respectively. There were differences in the content of flavonoids in different batches and growing areas. The three flavonoids were metabolized in various degrees after incubation of rat and human liver S9, cytosol, microsomes or intestinal flora in vitro, especially HES. The results of 16S rRNA showed that the main flavonoids of Citrus reticulata 'Chachi' could regulate lipid metabolism by regulating intestinal flora related to energy metabolism. This study established a rapid, simple, reproducible and stable quantitative analysis method for detecting the main flavonoids in Citrus reticulata 'Chachi' which evaluated the content of flavonoids from Citrus reticulata 'Chachi' in different growing areas and different storage periods. The intestinal bacteria can metabolize and transform the flavonoids of Citrus reticulata 'Chachi' to varying degrees, which provides a valuable scientific basis for the subsequent study on the material basis of the efficacy of Citrus reticulata 'Chachi' from the perspective of metabolism. Animal experiments were approved by the Medical Ethics Committee of Guangdong Jiangmen Chinese Medicine College (No. 20190419).
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O BJECTIVE To optimize stir-frying with saltwater technology of Citrus reticulata . METHODS Taking the contents of limonin ，nomilin and obacunone ，color difference value and free radical scavenging rate of 1，1-diphenyl-2-trinitrophenyl hydrazine （DPPH） as the indexes ，the entropy weight method was used for comprehensive evaluation. The stir-frying with saltwater technology of C. reticulata was optimized by central composite design-response surface method by using water-salt ratio ，stewing time，frying temperature and frying time as factors. RESULTS The optimal stir-frying with saltwater technology of C. reticulata included water-salt ratio of 8 ∶ 1（mL/g），stewing time of 22 min，frying time of 9 min and frying temperature of 158 ℃. After three times of validation tests ，the average comprehensive score of the optimized technology was 92.35（RSD＝2.19％），and its relative error with the predicted value （93.25）was 1.10％. CONCLUSIONS The optimal stir-frying with saltwater technology is stable and feasible.
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Abstract Essential oils (EO) from aromatic and medicinal plants generally perform a diverse range of biological activities because they have several active constituents that work in different mechanisms of action. EO from Citrus peel have an impressive range of food and medicinal uses, besides other applications. EO from Citrus reticulata, C. sinensis and C. deliciosa were extracted from fruit peel and analyzed by GC-MS. The major constituent of EO under evaluation was limonene, whose concentrations were 98.54%, 91.65% and 91.27% for C. sinensis, C. reticulata and C. deliciosa, respectively. The highest potential of inhibition of mycelial growth was observed when the oil dose was 300 μL. Citrus oils inhibited fungus growth in 82.91% (C. deliciosa), 65.82% (C. sinensis) and 63.46% (C. reticulata). Anti-Sclerotinia sclerotiorum activity of 90% pure limonene and at different doses (20, 50, 100, 200 and 300 μL) was also investigated. This monoterpene showed to be highly active by inhibiting 100% fungus growth even at 200 and 300 μL doses. This is the first report of the in vitro inhibitory effect of natural products from these three Citrus species and its results show that there is good prospect of using them experimentally to control S. sclerotiorum, in both greenhouse and field conditions.
Resumo Óleos essenciais de plantas aromáticas e medicinais geralmente exibem uma gama diversificada de atividades biológicas, pois possuem vários constituintes ativos que atuam por meio de vários mecanismos de ação. Os óleos essenciais das cascas de Citrus têm uma variedade impressionante de usos em alimentos, medicamentos entre várias outras aplicações. Os óleos essenciais (OE) de Citrus reticulata, C. sinenses e C. deliciosa foram extraídos das cascas dos frutos e analisados por CG-EM. O limoneno foi o constituinte majoritário encontrado nos óleos essenciais avaliados, nas concentrações de 98,54%, 91,65% e 91,27% para C. sinensis, C. reticulata e C. deliciosa, respectivamente. Os maiores potenciais de inibição do crescimento micelial foi observado na dose de 300 µL dos óleos. Os óleos de Citrus inibiram em 82,91% (C. deliciosa), 65,82% (C. sinensis) e 63,46% (C. reticulata) o crescimento do fungo. A atividade anti-Sclerotinia sclerotiorum do limoneno 90% puro e em diferentes doses (20, 50, 100, 200 e 300 µL) foi também investigada e este monoterpeno demonstrou-se altamente ativo inibindo 100% o crescimento do fungo inclusive nas doses de 200 e 300 µL. Este é o primeiro relato sobre o efeito inibitório in vitro dos óleos essenciais destas três espécies de Citrus e os resultados deste estudo mostram que existe uma boa perspectiva de uso destes produtos naturais experimentalmente para controlar o S. sclerotiorum tanto em condições de estufa como em condições de campo.
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Ascomycota , Oils, Volatile , Citrus , Plant Oils , Limonene , FruitABSTRACT
OBJECTIVE： To optimize and improve the quality standard for Citrus reticulata formula granules. METHODS： Totally 13 batches of C. Reticulata formula granules from 4 different manufacturers were used as trial samples， and qualitative identification of hesperidin and nobiletin in the samples were carried out by TLC according to the method of 2015 edition of Chinese Pharmacopoeia （part Ⅳ）. The quantitative analysis of naringin， hesperidin， hesperetin， nobiletin and tangeretin in C. reticulatae formula granules were conducted by UPLC[The determination was performed on Waters Acquity UPLC BEH C18 column with mobile phase consisted of acetonitrile-0.2％ phosphoric acid aqueous solution （gradient elution）. The detection wavelength was set at 283 nm， and sample size was 3 μL]. RESULTS： The results of TLC showed that in the chromatograms of samples， same color spots were shown in the corresponding positions of the chromatogram of reference substance. The results of UPLC showed， that the linear range of naringin， hesperidin， hesperetin， nobiletin and tangeretin were 0.64-6.44， 15.78-157.80， 0.17-1.66， 2.08-20.85 and 2.04-20.43 μg/mL， respectively （all r≥0.999 2）； the limits of detection were 0.03， 0.33， 0.10， 0.20 and 0.06 μg/mL； the limits of quantitation were 0.07， 1.34， 0.20， 0.60 and 0.22 μg/mL. The average recoveries were 99.4％， 99.6％， 99.7％， 99.7％ and 99.7％ （n＝9）； RSDs of precision （n＝6）， stability （n＝7） and reproducibility （n＝6） tests were all≤2.03％； naringin was detected in only 3 batches of samples from one manufacturer （the content ranged from 0.067 3 to 0.069.6 mg/g）， while the other 4 components were detected in 13 batches of samples （the contents of them ranged 0.646 5-1.728 0， 0.102 6-0.290 5， 0.023 1-0.689 8， 0.018 2-0.270 7 mg/g）. CONCLUSIONS： In this study， the quality standard of C. reticulata formula granules was improved by qualitative and quantitative methods， and the contents of hesperidin， hesperetin， nobiletin and tangeretin were not less than 0.60， 0.10， 0.02 and 0.01 mg/g， respectively.
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Objective To carry out genetic polymorphism analysis of Citrus reticulata Blanco cv. chachiensis Tanaka and its relatives by using SCoT molecular marker method. Methods Five factors of Mg2+, dNTPs, TaqDNA polymerase, primer, and template DNA concentration were used to screen the suitable SCoT-PCR reaction system for C. reticulata and its relatives by the method of orthogonal design. The optimum annealing temperature was screened by gradient temperature, and its polymorphic primers were verified. Results A total of 12 clear and rich bands were finally screened out as the primers of SCoT molecular marker for C. reticulata and its relatives in the optimized PCR reaction system, and the genetic distance and the UPGMA clustering tree of C. reticulata and its relatives were got by NTSYS software analysis. Conclusion The optimized SCoT-PCR reaction system was validated by using three different places of citrus genomic DNA to obtain the polymorphism and the clear amplified bands, which showed that the SCoT molecular marker system of Citrus reticulata is stable and reliable. The results of cluster analysis were able to make a preliminary separation of 13 kinds of materials scientifically and intuitively in molecule level.
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Objective To study the chemical constituents from Pericarpium Citri Reticulatae (Citrus reticulata). Methods The chemical constituents were isolated and purified by silica gel column, Sephadex LH-20, and ODS column chromatography. The structures were identified by spectral data. Results Nineteen compounds were isolated and identified as 4′,5,6,7-tetramethoxyflavone (1), 3,3′,4′,5,6,7,8-heptmethoxyflavone (2), sinensetin (3), 5-O-demethylnobiletin (4), tangeretin (5), nobiletin (6), apigenin (7), 5-O-desmethyltangeretin (8), 5,7-dihydroxy-3,3′,4′,6-tetramethoxyflavone (9), pachypodol (10), 4′,5,6,7-tetramethoxyflavanone (11), 3′,4′,5,7,8-pentamethoxyflavanone (12), agestricin C (13), scoparone (14), isoscopoletin (15), hesperidin (16), didymin (17), methylhesperidine (18), and naringin (19). Conclusion Compounds 9-15 are obtained from this plant for the first time.
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Objective To investigate the effects of different extracts of pericarpium citri reticulatae (PCR) and pogostemon cablin benth (PCB) on the contraction of gastrointestinal smooth muscle and the level of gastrointestinal hormones in rat model of gastrointestinal motility disorder. Methods Seventy Wistar rats were randomly divided into groups of control, model, water extractive of PCR, hesperidin, water extractive of PCB, water extractive of PCB plus volatile oil and patchouli alcohol, ten rats in each group. Except the control group, the rest groups were established gastrointestinal motor disorder model via limb ischemia-reperfusion (LIR). After modeling rats of groups were intervened with corresponding extracts. The effects of different extracts on contraction amplitude of corpora ventriculi and small intestine smooth muscle were observed. The levels of gastrointestinal hormones including motilin (MOT), gastrin (GAS), cholecystokinin (CCK) and somatostatin (SS) were detected by radioimmunoassay. Results The contraction amplitudes of corpora ventriculi and small intestine smooth muscle were decreased (P<0.05), the serum level of GAS and plasma level of MOT were also significantly decreased, while CCK and SS levels in the gastric antrum were significantly increased in model group than those of the control group (P < 0.05). Water extractive of PCR, hesperidin, water extractive of PCB and water extractive of PCB +patchouli oil can increase the contraction amplitudes of corpora ventriculi and small intestine smooth muscle, increase the serum level of GAS and reduce levels of CCK and SS in the gastric antrum (P<0.05), whereas showed no influence in the plasma level of MOT (P>0.05)]. Compared with model group, patchouli alcohol showed no influence in the contraction of gastrointestinal smooth muscle and levels of MOT, GAS, CCK and SS (P>0.05). In the aspect of regulating the contraction of gastrointestinal smooth muscle and the level of GAS, CCK and SS, the pharmacological effect of PCR water extract was better than that of hesperidin (P<0.05), while water extractive of PCB+volatile oil was better than that of water extractive of PCB (P < 0.05). Conclusion The active ingredients of PCR and PCB have variant regulative effects on the contraction of gastrointestinal smooth muscle and the serum level of GAS, CCK and SS in the gastric antrum in rat model of gastrointestinal motility disorder.
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Abstract Essential oils (EO) from aromatic and medicinal plants generally perform a diverse range of biological activities because they have several active constituents that work in different mechanisms of action. EO from Citrus peel have an impressive range of food and medicinal uses, besides other applications. EO from Citrus reticulata, C. sinensis and C. deliciosa were extracted from fruit peel and analyzed by GC-MS. The major constituent of EO under evaluation was limonene, whose concentrations were 98.54%, 91.65% and 91.27% for C. sinensis, C. reticulata and C. deliciosa, respectively. The highest potential of inhibition of mycelial growth was observed when the oil dose was 300 L. Citrus oils inhibited fungus growth in 82.91% (C. deliciosa), 65.82% (C. sinensis) and 63.46% (C. reticulata). Anti-Sclerotinia sclerotiorum activity of 90% pure limonene and at different doses (20, 50, 100, 200 and 300 L) was also investigated. This monoterpene showed to be highly active by inhibiting 100% fungus growth even at 200 and 300 L doses. This is the first report of the in vitro inhibitory effect of natural products from these three Citrus species and its results show that there is good prospect of using them experimentally to control S. sclerotiorum, in both greenhouse and field conditions.
Resumo Óleos essenciais de plantas aromáticas e medicinais geralmente exibem uma gama diversificada de atividades biológicas, pois possuem vários constituintes ativos que atuam por meio de vários mecanismos de ação. Os óleos essenciais das cascas de Citrus têm uma variedade impressionante de usos em alimentos, medicamentos entre várias outras aplicações. Os óleos essenciais (OE) de Citrus reticulata, C. sinenses e C. deliciosa foram extraídos das cascas dos frutos e analisados por CG-EM. O limoneno foi o constituinte majoritário encontrado nos óleos essenciais avaliados, nas concentrações de 98,54%, 91,65% e 91,27% para C. sinensis, C. reticulata e C. deliciosa, respectivamente. Os maiores potenciais de inibição do crescimento micelial foi observado na dose de 300 µL dos óleos. Os óleos de Citrus inibiram em 82,91% (C. deliciosa), 65,82% (C. sinensis) e 63,46% (C. reticulata) o crescimento do fungo. A atividade anti-Sclerotinia sclerotiorum do limoneno 90% puro e em diferentes doses (20, 50, 100, 200 e 300 µL) foi também investigada e este monoterpeno demonstrou-se altamente ativo inibindo 100% o crescimento do fungo inclusive nas doses de 200 e 300 µL. Este é o primeiro relato sobre o efeito inibitório in vitro dos óleos essenciais destas três espécies de Citrus e os resultados deste estudo mostram que existe uma boa perspectiva de uso destes produtos naturais experimentalmente para controlar o S. sclerotiorum tanto em condições de estufa como em condições de campo.
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Objective: To investigate the correlation of water and different volume fractions of ethanol boiling point and saturated steam pressure when using the vacuum extraction technology, and to compare the impact on saturation steam pressure of the addition of different medicine materials (Andrographis paniculata, Carthamus tinctorius, Angelica dahurica, Uncaria rhynchophylla, Scutellaria baicalensis, Salvia miltiorrhiza, Citrus reticulata) or the addition of materials with different particle sizes. Methods: To obtain the theoretical value and measured value of saturated steam pressure under different boiling points by the combined method of theoretical calculation, experiment measurement, and statistical analysis Results: The regression equation for the saturated steam pressure (P) with the volume fraction of ethanol solvent (V) and the boiling point (T) was P = 76.467 1+0.035 2 T2+1.201 0 TV-30.749 4 V2-3.123 9 T-14.966 7 V. The results showed that measured values of the saturated steam pressure of pure solvent, water and different concentration of ethanol, was less than the theoretical value. After adding herbs, the measured value of saturated steam pressure was higher than the pure solvent. The saturated steam pressure would reduce exactly as the extraction temperature reaches a certain temperature after the herbs were added. Conclusion: The addition and crush of herbs have influence on the saturated vapor pressure of solvent with small range.
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Objective: To establish the qualitative analysis model for Guang Citrus Reticulata Pericarpium (GCRP) using near infrared spectroscopy, so as to establish a rapid method to identify GCRP. Methods: After collecting the near-infrared spectra of GCRP and Chuan Citrus Reticulata Pericarpium (CCRP), standard orthogonal variable transformation (SNV) was used as pretreatment and cluster analysis method was used to establish identification models. The model validation and external validation were made, and a GCRP analysis model was established using near-infrared spectroscopy technology. Results: In wavelength range of 4000-10000 cm-1, GCRP was able to be distinguished. The accuracy rate of internal validation was 100% and the accuracy rate of external validation was 90.91%. Conclusion: It is feasible to identify GCRP samples by near infrared spectroscopy technique.
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Objective: To cloning limonoid-UDP-glucosyl transferase (LGT) gene in Citrus reticulata, analyze the bioinformations of LGT, and compare the expression to provide the foundation for the composite and regulation mechanism of the active ingredient in Citri Reticulatae Semen. Methods: LGT gene was cloned the protein characteristics were analyzed using bioinformatics and constructing phylogenetic tree. The expression of LGT in the different parts was analyzed using real time-PCR. Results: A 1 530 bp LGT sequence in in C. reticulata, was obtained, which had a 1509 bp ORF and could encode 502 amino acids. And protein analysis and forecast of its secondary and tertiary structures were carried out. Gene expression analysis revealed the expression of citrus LGT was the lowest in semen, and was followed by skin, and the highest expression of fresh. Conclusion: LGT gene of C. reticulata, and is successfully cloned, analyzed, and expressed a basis for the study of matter in the synthesis and regulation of limonoids is provided.
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O objetivo do trabalho foi avaliar o efeito de diferentes concentrações de fécula de mandioca na vida útil pós-colheita de frutos 'Mexerica Poncã' durante o armazenamento em temperatura ambiente em substituição ao filme de policloreto de vinila (PVC). Os frutos foram imersos em suspensões aquosas de fécula de mandioca a 0; 1; 2 e 3% (m:v) acrescidas de 0,5mL L-1 de óleo mineral Assist (Bayer Cropscience, da empresa Hoechst Schering AgrEvo UK Ltd. - Inglaterra) ou recobertos com PVC de 14µm de espessura, e armazenados a temperatura ambiente. As amostragens foram realizadas no tempo zero (início do experimento) e a cada dois dias, por oito dias. Foram avaliadas a perda de massa fresca, rendimento de suco, sólidos solúveis (SS), acidez titulável (AT), relação SS/AT e teor de ácido ascórbico. A perda de massa fresca aumentou durante o armazenamento, sendo mais acentuada na dose de 2%. A redução no teor de ácido ascórbico foi maior nos frutos recobertos com filme de PVC. A concentração de fécula de mandioca a 1% foi a que proporcionou melhores resultados quanto à manutenção da qualidade físico-química de frutos de 'Mexerica Poncã' durante oito dias de armazenamento.
The objective of this study was to evaluate the effect of different concentrations of cassava starch in postharvest life of fruits 'Tangerine Poncã' during storage at room temperature instead the film of polyvinyl chloride (PVC). The fruits were immersed in aqueous suspensions of cassava starch at 0, 1, 2 and 3% (m/v) plus 0.5mL L-1 a mineral oil Assist (Bayer CropScience, company Hoechst Schering AgrEvo UK Ltd. - Inglaterra) and the film of polyvinyl chloride (PVC) of 14µm thick, and stored at room temperature. The samples were collected at time zero (day of the assembly of the experiment) and then every two days. We evaluated the weight loss, juice yield, soluble solids (SS), titratable acidity (TA), SS / TA and ascorbic acid. The weight loss increased during storage, being more pronounced at a dose of 2%. The reduction in ascorbic acid content was higher in fruits covered with PVC film. The concentration of cassava starch to 1% was the highest values for the maintenance of physical and chemical quality of fruits of 'Mexerica Poncã' during eight days of storage.
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Objective: To investigate the antimicrobial activity of volatile constituents of fruit peels of Citrus reticulata Blanco on clinically isolated pathogenic microorganisms. Methods: Extraction of volatile oil was carried out by Clevenger’s apparatus. Volatile chemical components were measured by GC-MS. Antimicrobial activity was carried by Agar well diffusion assay with reference to standard fluconazole and tetracycline. Results: The chemical composition of volatile oil of the fruit peels of Citrus reticulata Blanco (Rutaceae) of Delhi Region was composed mainly monoterpenes (99.1 %) constituting l-limonene (92.4 %), γ-terpene (2.6 %) andβ-phellandrene (1.8 %). The volatile oil showed antibacterial and antifungal activities against the clinically isolated pathogenic microbial strains Escherichia coli, Staphylococcus aureus, Aspergillus flavus, Aspergillus niger, Aspergillus fumigatus and Candida albicans under in vitro condition. Conclusions: The potential antimicrobial activity of volatile oil present in fruit peels of C. reticulata can be useful for treatment of skin disorder and/or in aroma. Therapy, it can be incorporated into cosmetic formulations.
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O florescimento e a produção de tangerinas são influenciados pela safra anterior. Assim, a inibição do florescimento excessivo pode evitar uma frutificação elevada e a exaustão das reservas da planta, contribuindo para uma produção mais uniforme ao longo dos anos. O objetivo deste trabalho foi avaliar o efeito da aplicação de giberelina na redução do florescimento em tangerinas 'Poncã' e na produção de frutos em duas safras consecutivas. Os resultados demonstram que a aplicação de ácido giberélico reduziu a emissão de flores e aumentou a porcentagem de pegamento de frutos em relação ao ano de florescimento excessivo. A aplicação de giberelinas cerca de 90-150 dias antes do pleno florescimento pode contribuir para redução do efeito da alternância de produção.
The florescence and the production of tangerines are influenced by the last production. So, the inhibition of an excessive florescence can avoid a great production and the exhaustion of the plant reserves, contributing to a more uniform production every year. The objective of this work was to evaluate the effect of the reduction of the florescence in Ponkan mandarin trees upon the production of fruits in two consecutive productions. The results demonstrated that the gibberellic acid application reduced the flower production and increased the percentage of fruit production in relation to the year with an excessive flowering. The application of the gibberellic acid around 90-150 days before the full flowering period can contribute to the reduction of the effect of the production alternance.
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As características ambientais e a exposição das plantas e frutas à insolação podem influenciar no crescimento das plantas e qualidade da produção. O trabalho foi realizado em um pomar comercial, com o objetivo de avaliar as características físico-químicas em relação à disposição na copa de frutas de tangerineira 'Ponkan', submetidas ao raleio químico. Foram avaliados quatro tratamentos, dispostos em esquema fatorial 2 x 2, no delineamento de blocos casualizados, com oito repetições. Os fatores foram as concentrações de Ethephon, 0 e 600 mg L-1 e dois quadrantes de localização das frutas na copa, leste e oeste. As frutas de tangerineira 'Ponkan', localizadas no quadrante oeste da copa, apresentaram tamanho e teor de sólidos solúveis maiores e menor acidez, quando comparadas com aquelas do quadrante leste. A aplicação de Ethephon para promover o raleio químico melhorou a qualidade das frutas de tangerineira 'Ponkan' em todas as posições analisadas na copa.
Plant growth and production quality can be influenced by environmental characteristics and sunlight exposure of plants and fruits. The objective of this study was to evaluate physico-chemical characteristics in relation to canopy distribution of 'Ponkan' mandarin fruits submitted to chemical thinning in a commercial orchard. Four treatments were tested, set in 2 x 2 factorial scheme with eight randomized block replications. The factors were Ethephon concentrations (0 and 600 mg L-1 and two quadrants of canopy fruit distribution (east and west). The 'Ponkan' mandarin fruits located in the west quadrant presented greater size and solid soluble content and lesser acidity, as compared to those in the east quadrant. Chemical thinning with Ethephon application improved the quality of the 'Ponkan' mandarin fruits in all the canopy positions analyzed.
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A 'Ponkan' (Citrus reticulata Blanco) é uma das tangerinas mais produzidas no Brasil e muita apreciada pelo consumidor brasileiro. Tendo em vista a sua alta aceitação, há preocupação em se produzir frutos de boa qualidade. Entre os fatores que interferem na qualidade dos frutos podemos citar, como exemplo, a radiação solar. Objetivou-se, neste trabalho, avaliar as características físicas e químicas de frutos de tangerina 'Ponkan', cultivados no oeste do Paraná e colhidos em dois quadrantes da planta, um de maior incidência solar (leste-oeste) e outro de menor incidência (norte-sul). Para as análises físicas determinou-se: o peso do fruto, da casca e dos gomos, diâmetro transversal e longitudinal. Nas análises químicas foram avaliados: o pH, teor de sólidos solúveis totais (SST), acidez total titulável (ATT), relação SST/ATT e teor de ácido ascórbico. Pôde-se verificar pelos resultados obtidos que os frutos desenvolvidos no quadrante leste-oeste das plantas, onde ocorre maior incidência solar, apresentaram maior peso (212,86 g) e diâmetro longitudinal (74,42 mm) quando comparados com frutos do quadrante de menor incidência solar (180,30 g e 67,75 mm, respectivamente). Porém, nas características químicas não observaram diferenças que pudessem interferir no sabor dos frutos.
'Ponkan' is one of the most produced tangerines in Brazil and very appreciated by Brazilian consumers. Having in view its acceptance, there is a concern in producing fruits of high quality. Among the factors that interfere with the quality of the fruits, we can mention, as an example, the solar radiation. This work had the aim of evaluating the physical and chemical characteristics of 'Ponkan' tangerine (Citrus reticulata Blanco), cultivated in the west of Paraná and harvested in two quadrants of the plant, one of higher solar incidence (east-west) and the other of lower incidence (north-south). The physical analyses were the weight of the fruit, peel, and segment, as well the transverse and longitudinal diameter. In the chemical assay we evaluated the pH, content of total soluble solids (TSS), total titratable acidity (TTA), TSS/TTA ratio and content of ascorbic acid. It was verified by the results obtained, that the fruits that grew at the quadrant east-west of the plants, where a higher solar incidence occurs, presented higher weight (212.86 g) and longitudinal diameter (74.42 mm) when compared to fruits of the quadrant of lower solar incidence (180.30 g and 67.75 mm, respectively). However, regarding the chemical characteristics, no difference was observed which could interfere with the flavor of the fruits.
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Este trabalho foi realizado com o objetivo de avaliar o efeito do ethephon sobre o raleio químico das frutas de tangerineira "Ponkan". O ethephon foi aplicado nas tangerineiras em dois estádios de desenvolvimento das frutas: um e dois cm de diâmetro, sendo testadas quatro concentrações: 0,0; 100; 200 e 400mg L-1. Foram utilizadas três plantas úteis por parcela, com quatro repetições, perfazendo um total de 96 plantas. A qualidade das frutas foi avaliada a prtir das características: massa fresca da fruta, diâmetro transversal e longitudinal da fruta, número de gomos, número de sementes, sólidos solúveis (°Brix), pH do suco, acidez titulável, relação sólidos solúveis/acidez (ratio) e produção. A aplicação de ethephon na concentração de 400mg L-1, no estádio de desenvolvimento de dois cm para o raleio químico da tangerineira "Ponkan" proporciona o melhor resultado com maior massa fresca das frutas, quando comparado com o resultado da não aplicação do produto.
The aim of this research was to evaluate the ethephon effect on chemical thinning of the mandarin fruits 'Ponkan'. The ethephon was applied in the plants of mandarin in two development stages of the fruits: one and two cm of diameter, being tested four concentrations, 0,0; 100; 200 and 400mg L-1. Three experimental units with four repetitions were used, in a total of 96 plants. The quality of the fruits was evaluated with the characteristics of average weight of the fruit, transversal and longitudinal diameter of the fruit, corners and seed numbers, soluble solids (°Brix); pH of the juice, titratable acidity, ratio between °Brix and acidity (ratio) and production. The ethephon application for the chemical thinning of the mandarin 'Ponkan' at 400mg L-1 concentration in the development stage of two cm provide the best result with increase in weight of the fruits when compared to the non-chemical application.
ABSTRACT
Objetivou esta pesquisa testar o efeito da poda de topo no rebaixamento da copa e poda da saia, na recuperação da tangerineira 'Ponkan' com 12 anos de idade, altura de 4 metros, espaçadas de 6 x 4 m e enxertadas sobre limoeiro 'Cravo'. O experimento foi conduzido na Fazenda Vito Crincoli localizada no município de Perdões, MG. O delineamento experimental utilizado foi em blocos ao acaso, em esquema fatorial 4 x 2, sendo poda do topo (sem poda, poda a 3,0; 2,5 e 2,0m) e poda da saia (sem e com a poda) com quatro repetições. A parcela útil foi constituída de três plantas. As podas drásticas de topo prejudicaram a primeira produção, contudo a partir do segundo ano após a poda, as plantas apresentaram boa recuperação. Esse comportamento foi confirmado na terceira colheita, quando os diferentes tipos de podas do topo não se diferenciaram na produtividade, sendo que o tratamento com poda da saia foi superior ao sem poda.
This research aimed to test top pruning effect on the lowering of plant canopy, pruning the lower canopy on the recover of 12 years old 'Ponkan' tangerine tree, 4 meters height, 6 x 4 spaced and grafted on 'Cravo' lemon rootstock. This experiment was carried out at Vito Crincoli's Farm in Perdões, MG. It was carried out under randomized plots in a factorial scheme of 4x2, top pruning (without pruning, pruning at 3.0; 2.5 and 2.0 m) and skirt pruning (with and without pruning) with 4 replications. Plot size was composed by three plants. Heavy pruning of the top canopy lowered the first tree yield, however, and in the begining of the second year, the plants showed a very good recover. The same behavior was seem for the third harvest when several kinds of top pruning did not differ in the yield. Skirt pruning treatment was superior to that without any pruning.
ABSTRACT
Conduziu-se este trabalho para avaliar o efeito de duas temperaturas na qualidade de Tangerina 'Poncã', minimamente processada, durante 12 dias. O processamento mínimo consistiu de lavagem, sanificação, retirada das cascas das frutas e embalagem. Os frutos foram refrigerados a 0ºC e 10ºC. Foram avaliados, a cada três dias, as seguintes variáveis: concentração de O2 e CO2, perda de massa, valor L*, a* e b*, pH, acidez titulável, sólidos solúveis, rendimento do suco, vitamina C e beta-caroteno. Concluiu-se que, as temperaturas de 0ºC e 10ºC, não tiveram influência distinta sobre a cor, teores de vitamina C e beta-caroteno e sólidos solúveis, contudo influenciaram o rendimento do suco, que foi melhor nas frutas armazenadas a 10ºC. A interação temperatura e tempo afetaram a perda de massa e o pH, observando-se que, quanto maior a temperatura e o tempo de armazenamento, maiores são as perdas sofridas pelos frutos minimamente processados.
The objective of this work was to evaluate the effect of two temperatures on the quality of fresh-cut Tangerine 'Poncã' during 12 days. The minimal processing consisted of washing, sanitization, the peelling of the fruit and packing. The fruit were cooled at 0ºC and 10ºC. The following variable were evaluated every 3 days: concentration of O2 and CO2, mass loss, L *, a* and b * values, pH, titratable acidity, soluble solids, juiciness, vitamin C and beta-carotene. It was concluded that of temperature at 0ºC and 10ºC did not have different influence on color, vitamin C, beta-carotene and soluble solids, however held the juiciness what was the best in the fruit stored 10ºC. The interaction temperature and time affected the mass loss and pH, observing that the bigger temperature and the time of storage, the bigger are the losses suffered for the fresh-cut fruits.
ABSTRACT
Com o presente trabalho, objetivou-se analisar o comportamento da Tangerina 'Poncã' minimamente processada armazenada a 5°C. O experimento foi executado no Laboratório de Pós-Colheita de Frutas e Hortaliças do Departamento de Ciência dos Alimentos da Universidade Federal de Lavras MG. As frutas foram lavadas com detergente neutro e sanificadas com hipoclorito de sódio a 200 ppm por 15 minutos, descascadas, embaladas e resfriadas imediatamente a 5°C. Foi utilizado um delineamento inteiramente casualizado simples, com 3 repetições e 3 frutas por parcela experimental. A influência de 5 níveis do fator tempo (0, 3, 6, 9 e 12 dias) foi avaliada. A qualidade das frutas foi analisada a partir das seguintes variáveis: perda de massa, rendimento em suco, concentração de O2 e CO2 no interior das embalagens, valores L*, a* e b*, pH, acidez titulável, sólidos solúveis, beta-caroteno e vitamina C. A temperatura de 5°C foi adequada no armazenamento de Tangerina 'Poncã' minimamente processada, baseado nas pequenas modificações na cor, acidez titulável, sólidos solúveis, perda de massa e rendimento do suco, embora, perdas nos teores de vitamina C (34,07 por cento) e beta-caroteno (42,75 por cento) tenham sido observadas.
The objective of the work was to analyze the behavior of the fresh-cut 'Poncã' Tangerine stored 5°C. The experiment was carried out in the Postharvest Fruit and Vegetables Laboratory of the Food Science Department / Federal University of Lavras - Brazil. The fruit were washed with neutral detergent and sanitized with sodium hipochlorite 200ppm for 15 minutes, peeled, packed and immediately cooled at 5°C. It was used a fully randomly design, with 3 replications and 3 fruits per plote. The influence of 5 levels of the factor time (0, 3, 6, 9 and 12 days) was evaluated. The quality of the fruit was analyzed based in the following variable: mass loss, juiciness, concentration of O2 and CO2 into the packages, L *, a* and b * values, pH, titratable acidity, soluble solids, beta-carotene and vitamin C content. The temperature of 5°C was indicated to the storage of fresh-cut 'Poncã' tangerine based in minor changes in color, titratable acidity, soluble solids, mass loss and juiciness although losses in vitamin C (65,93 percent) and beta-carotene (42,75 percent) have been observed.