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OBJECTIVE To establi sh the fingerprint of Cnidium monnieri and a method for the content determination of 4 kinds of coumarins. METHODS Ultra-high performance liquid chromatography （UPLC） method was adopted to establish the fingerprints of 21 batches of C. monnieri ； their similarities were evaluated with Similarity Evaluation System of TCM Chromatographic Fingerprint （2012 edition）；common peaks were identification by comparison with reference substance. Using 10 common peak areas as variables ，cluster analysis was performed for 21 batches of C. monnieri by the method of between groups. The relative correction factors of xanthotoxin ，bergapten and imperatorin were calculated by the same UPLC method with osthole as the internal reference. The contents of them were calculated by quantitative analysis of multi-components by single marker （QAMS），and compared with the results of external standard method. RESULTS Totally 10 common peaks were identified in the fingerprints of 21 batches of C. monnieri ；the similarities ranged from 0.997 to 1.000. Peak 4 was identified as xanthotoxin ，peak 8 as bergapten ，peak 9 as imperatorin and peak 10 as osthole. A total of 21 batches of samples were divided into 3 categories，of which S 7 was clustered into one category ，S14 was clustered into one category ，and the other 19 batches were clustered into one category. The relative deviations of the contents of xanthotoxin ，bergapten and imperatorin determined by QAMS and external standard method were in the range of 0.88％ -1.07％ ，2.22％ -2.29％ ，0.67％ -2.93％ ，respectively. CONCLUSIONS UPLC fingerprint of C. monnieri is successfully established ，and QAMS method for content determination of 4 coumarins is also established.
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OBJECTIVE:To formulate the quality standard of Cnidium monnieri liniment (called "liniment" for short)preliminarily. METHODS:The property of liniment was observed. TLC was used for qualitative identification of osthole in liniment;relative density,alcohol content and pH value were also determined. The osthole and imperatorin in liniment were analyzed qualitatively by HPLC. RESULTS:3 batch of samples were reddish brown liquid and fragrant smelling. In TLC of test sample,the same color fluorescent spots were found in corresponding position as chromatogram of control sample. The linear range of osthole and imperatorin were 0.101 2-0.910 8(r=0.999 6)and 0.006 2-0.124 4 mg/L(r=0.999 6). RSDs of precision tests were 1.38% and 0.79%(n=6). RSDs of stability tests were 0.33% and 0.41%(n=6). RSDs of reproducibility tests were 0.83% and 1.98%(n=6),respectively. Average recoveries rate were 98.73%(RSD=1.29%,n=6)and 99.25%(RSD=1.22%,n=6).Results of content determination showed that the content of osthole and imperatorin were 2.20-2.35 mg/mL and 0.310-0.340 mg/mL,respectively. CONCLUSIONS:Established method is simple,rapid,accurate and can be used for quality control of C. monnieri liniment.
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Objective To identify the coumarins constituents in Cnidium monnieri and classify ten samples into three groups and this helpful chemical information could be used for the further pharmacological and clinical study on C. monnieri. Methods Qualitative analysis of coumarins in C. monnieri was detected by UHPLC-ESI-Q-TOF/MS. Quadrupole TOF/MS in either full scan mode or extracted ion mode was used for the qualitative analysis of the constituents. Relative peak area of each component was used for the hierarchical cluster analysis. Results According to UHPLC-ESI-QTOF-MS data, chemical structures of 28 coumarins in the fruits of C. monnieri were identified, including 19 simple coumarins, seven linear coumarins, and two angular coumarins. Among these constituents, ten coumarins were firstly identified in C. monnieri. In addition, hierarchical cluster analysis suggested that C. monnieri from different regions could be classified into four groups, and this clustering was correlated to the distribution significantly, Xuzhou could be regarded as the genuine producing area. Conclusion UHPLC-ESI-Q-TOF-MS is a viable method for qualitative analysis and quality evaluation of coumarins from the fruit of C. monnieri. Coumarins in C. monnieri exists intra-species variance, which indicates significant meaning for the quality control and choice of famous region drug for C. monnieri in the clinic medication.
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Objective: To study the chemical constituents from the fruits of Cnidium monnieri and their effects on proliferation of UMR106 cells. Methods: The constituents were separated by column chromatography, and their structures were elucidated by spectroscopic data analyses. The proliferation of all isolated compounds on osteoblast-like UMR106 cells was determined. Results: Nine compounds were isolated and identified as 5,7-dihydroxy-6,8-dimethoxy-2-methyl-4H-chromen-4-one (1), 5-hydroxy-2- hydroxymethyl-4H-chromen-4-one (2), (+)-marmesin (3), bergaptol (4), isobergapten (5), 7-hydroxy-8-(2',3'-dihydroxy-3'-methyl-butyl)-coumarin (6), murraol (7), coniferyl aldehyde (8), and m-hydroxybenzoic acid (9). Compounds 1, 3, and 7 showed the significant proliferative activities on UMR106 cells lines at the concentration of 1 × 10-10 mol/L and the proliferative ratios were 31.55%, 32.39% and 30.87%. Conclusion: Compounds 1-6 are isolated from the specie of Cnidium Cusson for the first time. Compounds 1, 3, and 7 increase UMR106 cells proliferation to some extent.
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Objective:To study the quality standard for Fujie lotion. Methods:The qualitative identification of Sophora alopecu-roides L, Cnidium monnieri ( L. ) Cuss and borneol was detected by TLC. The quantitative determination of matrine was detected by HPLC. Results:The identification by TLC was highly specific, and the content determination method was accurate and repeatable. The linear range of matrine was 0. 013 0-1. 30 mg·ml-1, and the average recovery was 97. 1% with RSD of 1. 7%(n=6). Conclu-sion:The standard can effectively control the quality of Fujie lotion.
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Objective: To study the chomenes from the fruit of Cnidium monnieri and their effects on proliferation of UMR106 cells. Methods: The constituents were separated by column chromatography, and their structures were elucidated by spectroscopic data analyses. The effects of all isolated compounds on proliferation of osteoblast-like UMR106 cells were determined. Results: Ten compounds were isolated and identified as cnidimoside A (1), cnidimol B (2), peucenin (3), 5,7-dihydroxychromone (4), 5-O-methylvisamminol (5), 4'-O-β-D-glucosyl-5-O-methylvisamminol (6), hamaudol (7), 2,5-dimethyl-7-hydroxychromone (8), cimifugin (9), and 5-hydroxy-chromone-7-O-β-D-glucoside (10). Compounds 1,5,9, and 10 showed the significant proliferative activities against UMR106 cells lines at the concentration of 0.10 nmol/L and the proliferative ratios were 30.23%, 31.56%, 35.29%, and 33.36%. Conclusion: Compounds 3-10 are isolated from species of genus Cnidium Cuss. for the first time. Compounds 1,5,9, and 10 (0.10 nmol/L) could increase the proliferation of UMR106 cells to some extent.
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Objective:To investigate the liposoluble constituents in fruit of Cnidium monnieri. Methods:The liposoluble constitu-ents in fruit of C. monnieri were extracted by Soxet extraction. The extracted liposoluble constituents were methyl esterified and then analyzed by GC-MS for the first time. Results:Thirty-nine compounds (85. 33%) were identified from the liposoluble constituents in fruit of C. monnieri. Phytol (15. 98%), hexadecanoic acid (12. 73%), 9,12,15-octadecatrienoic acid (11. 02%), 9-octadecenoic acid (6. 33%) and 9,12-octadecadienoic acid (4. 77%) were the main constituents of the liposoluble constituents in fruit of C. mon-nieri. Conclusion:Phytol, hexadecanoic acid, 9,12,15-octadecatrienoic acid and 9-octadecenoic acid are the active ingredients in fruit of C. monnieri.
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The aim of this study was to investigate the inhibitory effect of Cnidium monnieri fruit (CM) extracts on pulmonary inflammation induced in mice by cigarette smoke condensate (CSC) and lipopolysaccharide (LPS). Pulmonary inflammation was induced by intratracheal instillation of LPS and CSC five times within 12 days. CM extract was administered orally at a dose of 50 or 200 mg·kg(-1). The number of inflammatory cells in the bronchoalveolar lavage fluid was counted using a fluorescence activated cell sorter. Inflammatory mediator levels were determined by enzyme-linked immunosorbent assay. The administration of LPS and CSC exacerbated airway hyper-responsiveness (AHR) and induced an accumulation of inflammatory cells and mediators, and led to histological changes. However, these responses are modulated by treatment with CM, and the treatment with CM extract produces similar or more extensive results than the treatment with cyclosporin A (CSA). CM extract may have an inhibitory effect on pulmonary inflammation related with chronic obstructive pulmonary disease.
Subject(s)
Animals , Female , Anti-Inflammatory Agents , Pharmacology , Therapeutic Uses , Bronchoalveolar Lavage Fluid , Cnidium , Fruit , Lipopolysaccharides , Mice, Inbred BALB C , Phytotherapy , Plant Extracts , Pharmacology , Therapeutic Uses , Pneumonia , Drug Therapy , Pulmonary Disease, Chronic Obstructive , Drug Therapy , Pathology , Smoke , Smoking , Tobacco ProductsABSTRACT
OBJECTIVE:To analyze the chemical components of the essential oil from Cnidium monnieri from Anhui versus Henan by GC-MS.METHODS:The essential oil was extracted from Cnidium monnieri by steam distillation;the chemical components of the essential oil were detected by GC-MS;the relative contents of the chemical components were computed using area normalization method.Peaks were separated by capillary GC-MS and their corresponding compounds were identified.RESULTS:36 chief components from Cnidium monnieri from Anhui and 45 from that from Henan were identified.28 components were noted in both.There was great difference between the two Cnidium monnieri.CONCLUSION:This study serves as a scientific basis for the further exploitation and utilization of Cnidium monnieri.
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Object To isolate the active compounds on reversing multidrug resistance (MDR) of tumor cell from the ethanol extract in the seeds of Cnidium monnieri (L ) Cuss Methods The fractionation directed by bioactivity was carried out with silica gel chromatography and RP HPLC Results Three active coumarins were obtained: imperatorin (Ⅰ), edultin (Ⅱ) and 3′ isobutyryloxy O acetyl columbionetin (Ⅲ) Their sturctures were identified by spectroscopic analysis Conclusion These three compounds have a medium reversing MDR of KBV200 in vitro
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Object To estabish a method for the quantitative analysis of osthol in LOTIO FRUCTUS CNIDII COMPOSITA by RP HPLC Methods Megestrol acetate was used as the internal standard Results Chromatographic separation of osthol and megestrol acetate was accomplished within 12 min on Hypersil ODS 2(200 mm? 4 0 mm, 5 ?m) column and acetonitrile 0 01 mol/L sodium dihydrogen phosphate (48∶52) as mobile phase The flow rate was 1 2 mL/min and the detection wavelength was 320 nm A good linearity was obtained in the range of 0 123 8～2 970 ?g/mL (r=0 999 6) for osthol and the average method recovery was (98 4?0 90) % with RSD=0 91% Conclusion The method was simple, rapid, accurate, reliable, and suitable for the quality control of LOTIO FRUCTUS CNIDII COMPOSITA
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AIM: To set up a rapid analysis and characterization method for coumarins in extract of Cnidium monnieri. METHODS: The sample was analyzed by ultra-performance liquid chromatography with a reversed phase C_(18) column using a binary eluent of acetonitrile and water(with 0.1% formic acid) under gradient conditions.The ~+ ions of coumarins in Cnidium monnieri extract were observed by positive ion electrospray time of flight mass spectrometry (ESI-TOF-MS) online detection,and the fragmentation behavior obtained by ESI-TOF-MS/MS,then coumarins was identified through accurate molecular weight and molecular formula and fragmentation information combined with literature review. RESULTS: All components could be separated rapidly within 10 min,and five coumarins-xanthotoxol,bergapten,xanthotoxin,imperatorin and osthol could be primary identified in Cnidium monnieri extract. CONCLUSION: This method is effective for identification of coumarins in extract of Cnidium monnieri.
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AIM: To use secondary regression coherent design to explore the extraction parameters of fruits of Cnidiunm by supercritical CO_2 extraction. METHODS: Extraction pressure,extraction temperature,isolation pressure,isolation temperature Ⅰ and Ⅱ were viewed as factors. RESULTS: The best technological conditions were: extractive kettle pressure was 20 MPa,extractive kettle temperature was at 40(?C);separate kettle Ⅰ pressure was 15 MPa,separate kettle Ⅰ temperature was at 65(?C);separate kettle Ⅱ pressure was 5 MPa,separate ketle Ⅱ temperature was at 38(?C);CO_2 flow rate was 18 L/H,extractive time was 80 min. CONCLUSION: Under these conditions,the separate rate of the extractives from fruits of Cnidium Monnieri(L.) Cuss.could be(24.03%).Using secondary regression coherent design could naturally react the influence of the rule on the every factor to the goal function.