ABSTRACT
Objective: To investigate the chemical constituents from the pericarps of Aquilaria yunnanensis. Methods: The chemical constituents were separated and purified by silica gel, Sephadex LH-20 column chromatography, and semi-preparative HPLC. The structures of isolated compounds were identified by physicochemical properties and spectroscopic data. Results: Thirteen compounds were isolated from the ethyl acetate layer of 95% EtOH extract of the pericarps of A. yunnanensis, and identified as trans-linalool-3,6-oxide-7-O-β-D-(6'-O-acetyl)-glucoside (1), phenethyl-8-O-β-D-(6'-O-acetyl)-glucoside (2), mangiferin (3), iriflophenone-3,5-C-β-D-diglucoside (4), kaempferol-3-O-β-D-glucoside (5), luteolin-7-O-β-D-glucoside (6), isorhamnetin-3-O-β-D- glucoside (7), kaempferol-3-O-β-D-(6″-p-coumaroyl)-β-D-glucoside (8), geraniol-1-O-β-D-glucoside (9), 3-[2-formyl-5- (hydroxymethyl)-1H-pyrrol-1-yl] pentanedioic acid (10), cannabisin D (11), icariside D2 (12), and coniferin (13). Conclusion: Compound 1 is a new compound and compound 2 is a new natural product. Compounds 7, 9-13 were obtained from the Aquilaria genus for the first time. All compounds were firstly isolated from A. yunnanensis.
ABSTRACT
Objective: To study the chemical constituents from the stems of Clausena lansium. Methods: The chemical constituents were separated and purified by macroporous resin, silica gel, ODS column chromatography, Sephadex LH-20, and preparative HPLC. Their structures were determined by the analyses of ultraviolet spectrum, mass spectrum, and nuclear magnetic resonance spectroscopy. Results: Fifteen compounds were isolated from the n-BuOH fractions of 95% ethanol extract from the stems of C. lansium, and their structures were identified as 1, 1', 1″, 1‴, 1″″-tricontane lactam (1), 4-hydroxy-2, 6-dimethoxyphenyl 6'-O-syringoyl-β-D- glucopyranoside (2), 4-hydroxy-2, 6-dimethoxyphenyl 6'-O-vanilloyl-β-D-glucopyranoside (3), 4-hydroxymethyl-2-methoxyphenyl- 6'-O-syringoyl-β-D-glucopyranoside (4), 4-hydroxy-2-methoxyphenyl-6-O-syringoyl-β-D-glucopyranoside (5), syingin (6), coniferin (7), 3, 4, 5-trimethoxyphenyl-O-β-D-glucopyranoside (8), tinotuberide (9), trans-isoconiferin (10), phenethyl-O-β-D-glucopuranoside (11), araliopsine (12), geibalansine (13), integriquinolone (14), and γ-fagarine (15). Conclusion: Compounds 1-14 are isolated from this plant for the first time, compounds 1-11 are isolated from the plants of genus Clausena L. for the first time, and compound 1 is a new natural product.
ABSTRACT
Objective: To isolate phenyl propanoides from the barks of Syringa dilate, identify their structures, and determine the contents by reliable HPLC. Methods: Phenyl propanoides were extracted with methanol, and isolated by silica gel chromatography. The structures were elucidated by spectroscopic analysis including NMR, MS, UV, and IR. Shimpack CLC C18 (150 mm × 6 mm, 5 μm) column chromatography was employed with gradient elute of 1% acetic acid and methanol, and the column temperature was set at 30℃. The flow rate was 1.0 mL/min and the detection wavelength was set at 267 nm. Results: Two phenyl propanoides were isolated from the barks of S. dilata grown in DPR of Korea and identified as syringin (1) and coniferin (2). The linear range of syringin was 0.2-6.0 mg/mL, the correlation co-efficient was 0.999 8, and the average recoveries were 99.8%, 100.6%, and 100.9%. The results showed that the content in the barks was 8 folds of inner parts. There was no difference in locations and the content of syringin increased in winter, while decreased in summer. Conclusion: The elucidation of phenyl propanoides existence in S. dilata could be a fundamental for the search of new herbal materials. The method is simple, rapid, and reliable. It can be used to determine the content of syringin in herbal medicines.
ABSTRACT
OBJECTIVE: To study the chemical constituents of Ginkgo biloba kernel. METHODS: The chemical constituents were isolated by silica gel chromatography, and their structures were elucidated by physical-chemical and spectroscopic data. RESULTS: Nineteen compounds were isolated and their structures were identified as hexacosanoic acid (I), palmitic acid (II), ginnol(III), β-sitosterol (IV), 2, 3-dihydroxypropyl hexadecoate (V), ursolic acid(VI), sciadopitysin (VII), ginkgetin (VIII), isoginkgetin (IX), daucosterol(X), ginkdolide A (XI), ginkdolide B (XII), ginkdolide C (XIII), uracil(XIV), coniferin (XV), liquirtin (XVI), adenosine (VII), D-glucose (XVIII) and sucrose (XIX). CONCLUSION: Compounds VI, XV-XVII are obtained from this plant for the first time, compounds I-V, VII-X and XIV are obtained from Ginkgo biloba kernel for the first time. Copyright 2012 by the Chinese Pharmaceutical Association.