Effect of Guizhi Fuling Capsule on Apoptosis of Myeloma Cells Through Mitochondrial Apoptosis Pathway / 中国结合医学杂志

OBJECTIVE@#To observe the effects of Guizhi Fuling Capsule (GZFLC) on myeloma cells and explore the mechanisms.@*METHODS@#MM1S and RPMI 8226 cells were co-cultured with different concentrations of serum and the cell experiments were divided into negative (10%, 20% and 40%) groups, GZFLC (10%, 20%, and 40%) groups and a control group. Cell counting kit-8 (CCK-8) assays and flow cytometry were used to detect the viability and apoptosis levels of myeloma cells. The effects on mitochondria were examined by reactive oxygen specie (ROS) and tetrechloro-tetraethylbenzimidazol carbocyanine iodide (JC-1) assays. Western blot was used to detect the expression of B cell lymphoma-2 (Bcl-2), Bcl-2-associated X (Bax), cleaved caspase-3, -9, cytochrome C (Cytc) and apoptotic protease-activating factor 1 (Apaf-1). RPMI 8226 cells (2 × 107) were subcutaneously inoculated into 48 nude mice to study the in vivo antitumor effects of GZFLC. The mice were randomly divided into four groups using a completely randomized design, the high-, medium-, or low-dose GZFLC (840, 420, or 210 mg/kg per day, respectively) or an equal volume of distilled water, administered daily for 15 days. The tumor volume changes in and survival times of the mice in the GZFLC-administered groups and a control group were observed. Cytc and Apaf-1 expression levels were detected by immunohistochemistry.@*RESULTS@#GZFLC drug serum decreased the viability and increased the apoptosis of myeloam cells (P<0.05). In addition, this drug increased the ROS levels and decreased the mitochondrial membrane potential (P<0.01). Western blot showed that the Bcl-2/Bax ratios were decreased in the GZFLC drug serum-treated groups, whereas the expression levels of cleaved caspase-3, -9, Cytc and Apaf-1 were increased (all P<0.01). Over time, the myeloma tumor volumes of the mice in the GZFLC-administered groups decreased, and survival time of the mice in the GZFLC-administered groups were longer than that of the mice in the control group. Immunohistochemical analysis of tumor tissues from the mice in the GZFLC-administered groups revealed that the Cytc and Apaf-1 expression levels were increased (P<0.05).@*CONCLUSION@#GZFLC promoted apoptosis of myeloma cells through the mitochondrial apoptosis pathway and significantly reduced the tumor volumes in mice with myeloma, which prolonged the survival times of the mice.

Studies on chemical constituents in Guizhi Fuling Capsule (VII) / 中草药

Objective To study the chemical constituents from the n-butanol extract of Guizhi Fuling Capsule. Methods The chemical constituents were isolated and purified by multiple chromatographic methods. Their structures were identified on the basis of the spectral data and physicochemical properties. Results Five compounds were identified as 3'-O-galloylsucrose (1), 4'-O-galloylsucrose (2), 6'-O-galloylsucrose (3), 1'-O-galloylsucrose (4), and 1,2,3,4,6-pentagalloylglucose (5). Conclusion Compounds 1-5 are isolated from Guizhi Fuling Capsule for the first time, and compound 1 is a new compound. Compounds 1-5 inhibited calcium influx in uterine smooth muscle cells of primary mice.

An UPLC-MS/MS method for simultaneous determination of multiple constituents in Guizhi Fuling capsule with ultrafast positive/negative ionization switching / 中国天然药物

Guizhi Fuling capsule (GFC), a traditional Chinese medicine (TCM) with effects of promoting blood circulation and dissipating blood stasis, has been widely used in the clinic. Because of the complex matrix and various chemical structure types, quality control of GFC remains great challenge. In the present study, an ultra performance liquid chromatography hybrid triple-quadrupole mass spectrometry (UPLC-QQQ MS) method with ultrafast positive/negative ionization switching was developed for simultaneous determination of 18 bioactive components in GFC, including methyl gallate, ethyl gallate, oxypaeoniflorin, benzoic acid, albiflorin, paeonolide, paeoniflorin, 1, 2, 3, 4, 6-pentagalloylglucose, mudanpioside C, benzoyloxypaeoniflorin, benzoylpaeoniflorin, pachymic acid, amygdalin, cinnamaldehyde, paeonol, cinnamic acid, 4-hydroxybenzoic acid, and gallic acid. Separation was performed on an Agilent Zorbax Extend-C18 column (2.1 mm × 50 mm, 1.8 μm), using a gradient elution with acetonitrile and water containing 0.1% formic acid. Cholic acid was selected as the internal standard. This newly developed method was fully validated for linearity, precision, accuracy, and stability, and then applied to quality assessment of GFC. Finally, the batch-to-batch reproducibility of GFC samples was evaluated by the cosine ration and Euclidean distance method, which showed high quality consistency. The results demonstrated that the developed method pro vided a reasonable and powerful manner for quality control of GFC.

An UPLC-MS/MS method for simultaneous determination of multiple constituents in Guizhi Fuling capsule with ultrafast positive/negative ionization switching / 中国天然药物

Guizhi Fuling capsule (GFC), a traditional Chinese medicine (TCM) with effects of promoting blood circulation and dissipating blood stasis, has been widely used in the clinic. Because of the complex matrix and various chemical structure types, quality control of GFC remains great challenge. In the present study, an ultra performance liquid chromatography hybrid triple-quadrupole mass spectrometry (UPLC-QQQ MS) method with ultrafast positive/negative ionization switching was developed for simultaneous determination of 18 bioactive components in GFC, including methyl gallate, ethyl gallate, oxypaeoniflorin, benzoic acid, albiflorin, paeonolide, paeoniflorin, 1, 2, 3, 4, 6-pentagalloylglucose, mudanpioside C, benzoyloxypaeoniflorin, benzoylpaeoniflorin, pachymic acid, amygdalin, cinnamaldehyde, paeonol, cinnamic acid, 4-hydroxybenzoic acid, and gallic acid. Separation was performed on an Agilent Zorbax Extend-C18 column (2.1 mm × 50 mm, 1.8 μm), using a gradient elution with acetonitrile and water containing 0.1% formic acid. Cholic acid was selected as the internal standard. This newly developed method was fully validated for linearity, precision, accuracy, and stability, and then applied to quality assessment of GFC. Finally, the batch-to-batch reproducibility of GFC samples was evaluated by the cosine ration and Euclidean distance method, which showed high quality consistency. The results demonstrated that the developed method pro vided a reasonable and powerful manner for quality control of GFC.

Guizhi Fuling capsule / pill treatment for chronic pelvic inflammatory disease：a systematic review of randomized clinical trials / 中国中药杂志

This systematic review aims to systematically evaluate the efficacy and safety of Guizhi Fuling Capsule/Pill on the treatment of chronic pelvic inflammatory disease. We searched CNKI datebases,WanFang,SinoMed,PubMed,Embase,The Cochrane Library from the inception to February 2017,to collect randomized controlled trials（RCTs）of Guizhi Fuling Capsule/Pill in treating chronic pelvic inflammation disease.Two reviewers independently screened literature,extracted date and assessed the risk of bias of included studies.Then,the risk assessment of included references was evaluated according to criteria recommended by Cochrane Handbook 5.3.A total of 30 RCTs involving 3 586 patients were finally included.30 studies reported the clinical efficacy,the result of meta-analysis showed that：compared with the western medicine group,Guizhi Fuling (capsule,pill) combined with western medicine could significantly improve the clinical efficacy [RR=1.20,95%CI（1.16,1.23）];3 studies reported recurrence rate,the result of meta-analysis showed that：compared with the western medicine,Guizhi Fuling (capsule,pill) combined with western medicine could decrease the recurrence rate [RR=0.33,95%CI（0.18,0.62）]; Their secondary indicators mainly included hs-CRP, plasma viscosity ratio and tumor necrosis factor and fibrin,the result of meta-analysis showed that： Guizhi Fuling (capsule,pill) combined with western medicine was better than western medicine in terms of anti-inflammatory and improving blood circulation.17 studies reported adverse reactions,most of the adverse events were the irritation of gastrointestinal tract. The result showed that： compared with the western medicine group, the incidence of adverse reactions in Guizhi Fuling (capsule,pill) combined with western medicine group is low.Guizhi Fuling（capsule,pill） did not increase the adverse reactions. The available evidence suggests that： Guizhi Fuling（capsule, pill） compared with the western medicine group, Guizhi Fuling (capsule,pill) combined with western medicine was more effective than the western medicine group in terms of clinical efficacy, recurrence rate, anti-inflammatory and plasma viscosity.Due to the limited quality and quantity of included studies,more high quality RCTs are needed to verify the above conciusion.

Optimization of Flash Extraction Technology for Guizhi Fuling Capsule by Box-Behnken Design-response Surface Method / 中国药房

OBJECTIVE:To optimize the flash extraction technology for Guizhi fuling capsule. METHODS:Using blade speed,liquid-solid ratio,extraction time as investigation factors,using the comprehensive evaluation values consisting of transfer rates of gallic acid,paeoniflorin,benzoic acid,cinnamic acid,benzoyl paeoniflorin,amygdalin as investigation index,single fac-tor test was combined with Box-Behnken design-response surface method to optimize the flash extraction technology for Guizhi ful-ing capsule. Verification test was conducted and compared with conventional decoction extraction method(extracting twice,totally 240 min). RESULTS:The optimal flash extraction technology for Guizhi fuling capsule was using water as extraction solvent with blade speed of 5600 r/min and liquid-solid ratio of 10.5:1,extracting for 60 s. The average comprehensive evaluation value of veri-fication test was 85.40%(n=3),with relative error of 0.15% to the predicted value(85.55%),higher than decoction extraction method(72.65%). CONCLUSIONS:Optimized flash extraction technology for Guizhi fuling capsule is efficient and rapid.

Effect of Guizhi Fuling capsule and its extracts on human breast cancer cells proliferation / 中国药理学与毒理学杂志

OBJECTIVE To evaluate the effect of Guizhi Fuling Capsule active pharmaceutical ingredient (API) and its fractions on human breast cancer cells proliferation by high-throughput screening assay. METHODS The crude fractions were obtained from the extraction and elution of the API of Guizhi Fuling Capsule, and 929 standard fractions were obtained by the optimal separation conditions. Sulforhodamine B (SRB) method was used to evaluate the effects of the Guizhi Fuling capsule API and 929 kinds of fractions on the proliferation of human breast cancer cells MCF- 7 and MDA- MB- 231. RESULTS The Guizhi Fuling capsule API had a strong ability to inhibit the proliferation of MCF-7 cells at high concentration and the ability to inhibit MDA-MB-231 cells' proliferate at low concentration follow?ing 72 h treatment;some samples of 929 fractions (5 μg·mL-1) was found to have a breast cancer cell growth inhibition rate above 50%, without toxicity on HUVECs proliferation. CONCLUSION The API of Guizhi Fuling capsule had significant cytotoxicity effects on these two human breast cancer cells, with significant concentration- and time-dependent manner.

Effect of Guizhi Fuling capsule combined with blood activating and stasis removing therapy on uterine fibroids / 中国生化药物杂志

Objective To investigate the clinical effect of Guizhi Fuling Capsule Combined with activating blood circulation to remove stasis to treat uterine fibroids.Methods 84 patients with uterine fibroids from June 2015 to October 2016 in our hospital were randomly selected and divided into experimental group and control group, 42 cases in each group.The control group was treated with Guizhi Fuling Capsule,and the experimental group was treated with blood activating and Stasis Removing Therapy on this basis of treatment.The patients were followed up for three months, and the clinical treatment effects of the two groups were compared and analyzed.Results The total effective rate of the experimental group was 97.6%,which was significantly better than the control group(76.1%), the difference was statistically significant(P<0.05);through the change of single symptom in two groups, it was found that there was no significant difference between the experimental group and the control group;The improvement rate of anemia, vaginal bleeding, abdominal pain, dysmenorrhea, menorrhagia in the experimental group was significantly higher than the control group, the difference between the two groups was statistically significant(P<0.05);The average volume of uterine fibroids in the experimental group was significantly lower than that in the control group, the difference was statistically significant(P<0.05).Conclusion Guizhi Fuling Capsule Combined with promoting blood circulation and removing blood stasis therapy has obvious effect and less adverse reaction.It can be used and popularized in clinic.

Clinical Observation of Guizhi Fuling Capsule Combined with Leuprolide Acetate in the Treatment of Endo-metriosis after Laparoscopicsurgery / 中国药房

OBJECTIVE:To observe the efficacy and safety of Guizhi fuling capsule combined with leuprolide acetate in the treatment of endometriosis (EMS) after laparoscopicsurgery. METHODS:87 EMS patients were randomly divided into control group(44 cases)and observation group(43 cases). Control group received EMS resection under laparoscope,3.75 mg Leuprolide acetate for injection was given in the first day of postoperative first menstruation by intramuscular injection,once a day. Observa-tion group additionally received 0.93 g Guizhi fuling capsule,3 times a day. The treatment course for both groups was 6 months. Clinical efficacy,estradiol(E2),follicle stimulating hormone(FSH),luteinizing hormone(LH)and prolactin(PRL)levels before and after treatment,recurrence after 6 months and the incidence of adverse reactions in 2 groups were observed. RESULTS:The to-tal effective rate in observation group was significantly higher than control group,recurrence rate was significantly lower than con-trol group,the differences were statistically significant (P0.05);after treatment,E2,FSH,LH and PRL in 2 groups were significantly lower than be-fore,and E2,FSH and LH in observation group were lower than control group,the differences were statistically significant (P0.05). And there was no significant difference in the inci-dence of adverse reactions in 2 groups (P>0.05). CONCLUSIONS:After laparoscope resection,Guizhi fuling capsule combined with leuprolide acetate can effectively improve efficacy,reduce sex hormone level and recurrence rate,and do not increase the inci-dence of adverse reactions in the treatment of EMS.

Studies on chemical constituents in Guizhi Fuling Capsule (VI) / 中草药

Objective: To study the chemical constituents from the ethyl acetate and n-butanol extract of Guizhi Fuling Capsule. Methods: The chemical constituents were isolated and purified by multiple chromatographic methods. Their structures were identified on the basis of the spectral data and physicochemical properties. Results: Ten compounds were identified as 4-hydroxybenzoic acid (1), trans-O-methoxy cinnamic acid (2), trans-cinnamic acid (3), 4-hydroxycinnamic acid (4), 2,5-dihydroxy-4-methylacetophenone (5), ethyl gallate (6), vanillic acid (7), protocatechuic acid (8), affinoside (9), and benzyl-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside (10). Conclusion: Compounds 1-10 are isolated from this plant for the first time.

Molecular mechanism of Guizhi Fuling Capsule for treatment of dysmenorrhea, pelvic inflammatory disease, and hysteromyoma via network pharmacology / 中草药

Objective: To investigate the molecular maechanism and potential active constituents population of Guizhi Fuling Capsule (GFC) for the treatment of dysmenorrhea, pelvic inflammatory disease (PID), and hysteromyoma. Methods: One handred and thirty target proteins related with dysmenorrhea, PID, and hysteromyoma were selected through mining literature, retrieving in DrugBank and TTD database, the main active constituents and potential target proteins from GFC were computed and analyzed by DOVIS2.0 and Cytoscape 3.0. The potential target proteins were then projected into the KEGG databases to illustrate the molecular mechanism of GFC. Results: The results of data analysis showed that the 115 active molecules with stronger interaction with protein were distributed in Cinnamomi Ramulus and Poria. The High network degree and betweenness of molecules were found to be pentacyclic triterpenes and steroids by further analysis of network characteristics. The most of the potential target proteins (78.57%) interacted wth the compounds in GFC were from 15 biological pathways closely related with dysmenorrhea, PID, and hysteromyoma in KEGG database, which was involved in cell proliferation, angiogenesis, coagulation, dysregulation of inflammatory process, uterine contractions, and release of estrogen or progesterone in uterus. As well as the synthesis or release of inflammatory factors such as prostaglandin and the regulation of calcium channels and so on. Conclusion: GFC has the function by the interaction of pentacyclic triterpenes and steroids with multi target proteins, such as arachidonic acid metabolism, calcium signaling pathway, GnRH signaling pathway, complement and coagulation cascades, and progesterone-mediated oocyte maturation, to alleviate the pain of dysmenorrhea and PID, or improve the quality of life for the patients with hysteromyoma through inhibiting uterine contractions, improving microcirculation, and reducing the release of estrogen or promegestone and inflammatory response (such as PGE2, PGF2α, and leukotriene B4).

Effect ofGuizhi-Fulingcapsule on expression of AngⅡ, eNOS in rats with UUO-induced tubulointerstitial fibrosis / 国际中医中药杂志

Objectives To observe the effect ofGuizhi-Fuling capsule on the expression of AngⅡand eNOS.Methods 72 male Sprague-Dawley(SD) rats, aged three months, were randomly divided into three groups: a sham-operated group(n=24),a model group (n=24) and a treatment group (n=24). Renal tubulointerstitial fibrosis model was established via unilateral ureteral obstruction(UUO). Rats in the treatment group were treated withGuizhi-Fulingcapsule (125mg/kg),and the same volume of normal saline were given to the rats in the sham -operated group and the model group at the same time. Rats were put to death at day 7, 14, 21 Immunohisto-chemistry staining was performed to investigate both the expression of AngⅡ, eNOS in TIF. Results 1 Renal interstitium injury was seen in the model group and the treatment group rats at day 7. The degree of renal interstitium fibrosis in rats from the treatment group was lighter than that of the model group at day 7, 14, 21 (P< 0.01).2 At day 7,14 and 21, the expression of AngⅡin rats from the model group and the treatment group were higher than that of the sham-operated group (P<0.01), but those of the treatment group was lower than those of the model group (P< 0.01). The expression of AngⅡ in rats from the model and the treatment groups were increasing,the highest in the 21th day (P<0.01).3 At day 7, 14 and 21, the expression of eNOS in rats from the model group and the treatment group were lower than that of the sham-operated group  (P<0.01).ConclusionsGuizhi-Fuling capsule can alleviate the lesion of renal tissue in obstructive kidney and delay the development of TIF.

Clinical Observation of Guizhi Fuling Capsule Combined with Mifepristone in Treatment of Uterine Fibroids / 中国药房

OBJECTIVE:To observe the efficacy and safety of Guizhi fuling capsule combined with mifepristone in treatment of uterine fibroids. METHODS:116 patients with uterine fibroids were randomly divided into control group and observation group. Control group was orally given Mifepristone tablet 25 mg,2 h before meal in the first day of menstrual period,once a day,for con-tinuous 10 d;observation group was additionally given Guizhi fuling capsule 3 pills in non-menstrual period,3 times a day. 3 months was regarded as a treatment course,it lasted 2 courses. Clinical efficacy,and E2,FSH,SHBG,uterine volume,menstrual blood volume,uterine fibroid volume and incidence of adverse reactions in 2 groups before and after treatment were observed. RE-SULTS:Total effective rate in observation group was significantly higher than control group,the difference was statistically signifi-cant(P0.05). CONCLU-SIONS:The clinical efficacy of Guizhi fuling capsule combined with mifepristone in treatment of uterine fibroids is more signifi-cant than mifepristone alone,with good safety.

Clinical Observation of Guizhi Fuling Capsule Combined with Tamoxifen Citrate Tablet in the Treatment of Breast Hyperplasia / 中国药房

OBJECTIVE:To observe the clinical efficacy and safety of Guizhi fuling capsule combined with Tamoxifen citrate tablet in the treatment of breast hyperplasia. METHODS:216 female patients with breast hyperplasia were randomly divided into control group and observation group. Control group was orally treated with Tamoxifen citrate tablet 10 mg,bid. Observation group was additionally orally treated with Guizhi fuling capsule 3 granules,tid. The treatment course was 3 months. Drug withdrawal was conducted in menstrual period. The clinic data was observed,including clinical efficacy,the maximum mass diameter and breast pain scores before and after treatment,and incidence of adverse reactions. RESULTS:The total effective rate in observation group was significantly higher than control group,the difference was statistically significant(P0.05). CONCLUSIONS:Guizhi fuling capsule combined with Tamoxifen citrate tablet has more significant efficacy than only Tamoxifen citrate tablet in the treatment of breast hyperplasia,with similar safety.

Directional separation of active ingredients of dehydrotumulosic acid from Guizhi Fuling Capsule by molecular imprinting technique / 中草药

To establish a method for directional separation of dehydrotumulosic acid from the extracts of Guizhi Fuling Capsule with molecular imprinting technique (MIT). Molecular imprinting polymer (MIP) was prepared by sol-gel process with dehydrotumulosic acid as molecular template to study the absorption property. The dehydrotumulosic acid was achieved from Guizhi Fuling Capsule by one-step separation with polymer as filler. The structure of dehydrotumulosic acid was identified on the basis of the spectral data and physicochemical property. The maximum binding capacity (Qmax) of MIP was 9.10 mg/g measured by Scatchard equation and the purity of dehydrotumulosic acid was 90.76% by HPLC. The established MIT for the directional separation of dehydrotumulosic acid from Guizhi Fuling Capsule is simple and benefit to reducing the solvent use during the separation process, which could offer a novel method for the separation and purification of dehydrotumulosic acid.

Studies on chemical constituents in Guizhi Fuling Capsule (V) / 中草药

To investigate the chemical constituents from the ethyl acetate and n-butanol extact of Guizhi Fuling Capsule. The compounds were isolated by chromatography on silica gel, Sephdex LH-20 columns, and prep-HPLC. The chemical structures were identified by NMR methods, respectively. Eleven compounds were isolated from the ethyl acetate extact. They were identified as benzoylpaeoniflorin (1), albiflorin R1 (2), paeonidanin A (3), 4-methylbenzoylpaeoniflorin (4), paeonidanin B (5), 4-O-methylgalloylpaeoniflorin (6), and paeoniflorin B (7). Four compounds were isolated from the n-butanol extact and were identified as isomaltopaeoniflorin (8), paeoniflorin (9), oxypaeoniflorin (10), and albiflorin (11). Compounds 1-8 are isolated from Guizhi Fuling Capsula for the first time, and compounds 1-11 belong to the category of peaoniflorin.

Studies on chemical constituents of Guizhi Fuling Capsule (IV) / 中草药

Objective: To investigate the chemical constituents from the petroleum ether and ethyl acetate extract of Guizhi Fuling Capsule (GFC). Methods: The compounds were separated with chromatography on silica gel and polydextran gel columns, and prep-HPLC, and their chemical structures were identified by NMR and MS spectral methods, respectively. Results: Eight compounds were isolated from the petroleun ether extract and were identified as coumarin (1), 7-hydroxycoumarin (2), cinnamic alcohol (3), ergosterol (4), ergosta-4, 6, 8 (14), 22-tetraen-3-one (5), β-amyrin acetate (6), campesterol (7), and α-amyrin acetate (8). Thirteen compounds were isolated from the ethyl acetate extract and were identified as apingenin (9), kaemferol (10), quercetin (11), kaempferol-3-O-glucopyranoside (12), catechin (13), epicatechin (14), syringic acid (15), hyperin (16), eicosanoids (17), syringaresinol (18), mudanpioside A (19), mudanpioside C (20), and galloylpaeoniflorin (21). Conclusion: Compounds 1-21 are isolated from GFC for the first time.

Experimental study on anti-atherosclerosis effects of Guizhi Fuling Capsule / 国际中医中药杂志

Objective To observe the anti-atherosclerosis effects of Guizhi Fuling Capsule. Methods 50 healthy SD male rats were randomly divided into 2 groups: normal group (n=12) and AS model group(n=38). The model of experimental AS rats was established by feeding high cholesterol diet and intraperitoneal injecting VitD3. The normal control group was fed by ordinary diet. AS model group was further divided into 3 groups randomly: AS group(n=12), control group (n= 12), and therapy group(n= 12). The therapy group was given Guizhi Fuling Capsule by intragastric administration.The control group was given simvastatin and captopril by intragastric administration. 0.9% NaCl was given to the AS group instead of Guizhi Fuling Capsule. The rats were killed at the 16th weekend, and their aorta arch and breast aorta were observed.Blood fat and serum levels of Endothelin-1 (ET-1), Nitric oxide (NO), high-sensitivity C-reactive protein (hs-CRP) were detected respectively. Immunohistochemistry technology was used for observing the expression of intercellular adhesion molecule- 1 (ICAM- 1 ) in breast aorta. Results The values of ET- 1, NO, hs-CRP and the amount of ICAM- 1 were (142.61 ±25.67)ng/ml, (66.05 ± 8.63) μmol/L, (2.86±0.40)mg/L, (0.29±0.05) in the normal group; (182.38±22.96)ng/ml, (28.70±4.49)μmol/L, (5.60±0.49)mg/L, (0.58±0.06)in the AS group; (154.37±21.11)ng/ml, (45.88±11.36)μmol/L, (3.66±0.34)mg/L, (0.37±0.04)in the control group and (152.13±23.23)ng/ml, (57.67±8.96)μmol/L, (3.70±0.40)mg/L, (0.36±0.04) in the therapy group respectively. The levels of ET-1, NO, hs-CRP and the amount of ICAM-1 have significant difference between the AS group and the normal group(P=2.47E-4, 6.02E-19, 1.11E-12, 2.51E-18). There was also significant difference between the control group and the AS group(P=0.005, 1.59E-14, 1.87E-5, 3.87E-14). The level of NO in the therapy group was higher than that in the control group (P=0.002). The levels of ET-1, hs-CRP and the amount of ICAM-1 did not have significant difference between the therapy group and the control group(P=0.81, 0.81, 0.48) Conclusion Guizhi Fuling Capsule has effects of Anti-atherosclerosis on rats with AS.

Clinical Observation on Termination of Pregnancy Effects of 75 Patients Applied with Guizhi Fuling Capsule Combined with Mifepristone / 国际中医中药杂志

Objective To observe the effects of termination of pregnancy with Guizlfi Fuling Capsule plus Mifepristone.Methods 75 early pregnant women were randomly recruited into a treatment group and a control group.Women in the control group were applied with Mifepristone plus Misoprostoi.Form the 3rd day,women in the treatmcnt group were applied wilh Guizhi Fuling Capsule(3 times daily,and 3 granules for each time)for successive 7 days oil the basis of medicines used in the control group.Compared and analyzed the therapeutic effects of both groups.Results The average time for ovum evacuation in the control group and the tretment group was(6±1.8)h and(3±2.6)h separately.Besides both bleeding volume and bleeding time in the Izeatment group were lower than the eonlxol group.Conelusion Guizhi Fuling Capsule plus Mifeoristolle therapy will shorten ovum evacuation time and decrease bleeding volume.

Mifepristone Combined with TCD(Traditional Chinese Drug) Treat Tubal Pregnancy / 浙江中医药大学学报

[Objective]To observe the cure effect of Mifepristone(RU486) joined with TCD on tubal pregnancy. [Method] Randomly divide the said 134 patients into 2 groups, the control group 66 cases take MTX 50mg/㎡ for one-time muscular injection; the study group 68 Mifepristone 100mg/time, 2times/d, for successive 3 days, meanwhile added with TCD. Periodically measure both groups’ ?-HCG, test the mass reduction with B ultrasonic exam. [Result] For the study group, the cure rate was 91.2%; 83.3% for the control one, without statistical meaning; the ?-HCG negative rate and mass reduction rate were better in study group than the control one, with marked difference; moreover, the study group had less side effect, less in-hospital time. So, Mifepristone combined with TCD is an effective method for preserved treatment of tubal pregnancy.